DK595286A - Fremgangsmaade til fremstilling af thrombininhibitorer - Google Patents

Fremgangsmaade til fremstilling af thrombininhibitorer

Info

Publication number
DK595286A
DK595286A DK595286A DK595286A DK595286A DK 595286 A DK595286 A DK 595286A DK 595286 A DK595286 A DK 595286A DK 595286 A DK595286 A DK 595286A DK 595286 A DK595286 A DK 595286A
Authority
DK
Denmark
Prior art keywords
manufacturing
thrombin inhibitors
eukaryotic host
hybrid vectors
desulphatohirudin
Prior art date
Application number
DK595286A
Other languages
English (en)
Other versions
DK595286D0 (da
DK175492B1 (da
Inventor
Bernd Meyhack
Walter Maerki
Jutta Heim
Original Assignee
Ciba Geigy Ag
Christensen Plantorgan Werk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB858530631A external-priority patent/GB8530631D0/en
Application filed by Ciba Geigy Ag, Christensen Plantorgan Werk filed Critical Ciba Geigy Ag
Publication of DK595286D0 publication Critical patent/DK595286D0/da
Publication of DK595286A publication Critical patent/DK595286A/da
Application granted granted Critical
Publication of DK175492B1 publication Critical patent/DK175492B1/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
DK198605952A 1985-12-12 1986-12-11 Fremstilling af thrombininhibitorer DK175492B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB858530631A GB8530631D0 (en) 1985-12-12 1985-12-12 Thrombin inhibitors
GB8530631 1986-05-29
GB868613088A GB8613088D0 (en) 1985-12-12 1986-05-29 Thrombin inhibitors
GB8613088 1986-05-29

Publications (3)

Publication Number Publication Date
DK595286D0 DK595286D0 (da) 1986-12-11
DK595286A true DK595286A (da) 1987-06-13
DK175492B1 DK175492B1 (da) 2004-11-08

Family

ID=26290108

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198605952A DK175492B1 (da) 1985-12-12 1986-12-11 Fremstilling af thrombininhibitorer

Country Status (17)

Country Link
EP (1) EP0225633B1 (da)
JP (2) JPH0650990B2 (da)
AT (1) ATE79403T1 (da)
AU (1) AU615413B2 (da)
CA (1) CA1341496C (da)
DE (1) DE3686399T2 (da)
DK (1) DK175492B1 (da)
ES (1) ES2051688T3 (da)
FI (1) FI94773C (da)
GR (1) GR3006248T3 (da)
HU (1) HU209146B (da)
IE (1) IE57859B1 (da)
IL (1) IL80932A (da)
MY (1) MY101203A (da)
NO (1) NO178035C (da)
PH (1) PH31277A (da)
PT (1) PT83898B (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE64956T1 (de) * 1984-06-14 1991-07-15 Ciba Geigy Ag Verfahren zur herstellung von thrombininhibitoren.
MC1834A1 (fr) * 1986-07-10 1988-06-03 Transgene Sa Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
GB8620926D0 (en) * 1986-08-29 1986-10-08 Delta Biotechnology Ltd Yeast promoter
US6005071A (en) * 1987-01-23 1999-12-21 Merrell Pharmaceuticals Inc. Anticoagulant peptides
CA1341032C (en) * 1987-01-23 2000-06-20 John L. Krstenansky Anticoagulant peptides
US5789540A (en) * 1987-01-23 1998-08-04 Merrell Pharmaceuticals Inc. Anticoagulant peptides
US5256559A (en) * 1988-03-04 1993-10-26 Biogen, Inc. Methods and compositions for inhibiting platelet aggregation
GB8809860D0 (en) * 1988-04-26 1988-06-02 Ciba Geigy Ag Process for production of polypeptides
GB8907110D0 (en) * 1988-05-04 1989-05-10 Ciba Geigy Ag Improvements in the production of polypeptides
US5268296A (en) * 1988-06-11 1993-12-07 Ciba-Geigy Corporation DNA vector and recombinant host cell for production of hirullin P6 and P18
US5167960A (en) * 1988-08-03 1992-12-01 New England Deaconess Hospital Corporation Hirudin-coated biocompatible substance
US5112615A (en) * 1988-08-03 1992-05-12 New England Deaconess Hospital Corporation Soluble hirudin conjugates
GB8826428D0 (en) * 1988-11-11 1988-12-14 Biopharm Ltd Antithrombin
FR2646437B1 (fr) * 1989-04-28 1991-08-30 Transgene Sa Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
US5179196A (en) * 1989-05-04 1993-01-12 Sri International Purification of proteins employing ctap-iii fusions
DE4009268A1 (de) * 1990-03-22 1991-09-26 Consortium Elektrochem Ind Sekretion von hirudinderivaten
US6514730B1 (en) 1991-03-21 2003-02-04 Consortium für elektrochemische Industrie GmbH Secretion of hirudin derivatives
ES2152943T3 (es) * 1992-09-04 2001-02-16 Novartis Ag Procedimiento para la produccion de inhibidores de proteasas.
US5981227A (en) 1995-02-09 1999-11-09 Movartis Ag Process for the production of proteins
US7795205B2 (en) 2004-04-12 2010-09-14 Canyon Pharmaceuticals, Inc. Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
JP7284955B2 (ja) * 2020-07-20 2023-06-01 フォーデイズ株式会社 神経幹細胞の増殖促進剤及びそれを用いた神経幹細胞の増殖促進方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1984004330A1 (en) * 1983-04-22 1984-11-08 Amgen Secretion of exogenous polypeptides from yeast
DE3486452T2 (de) * 1983-10-03 1997-11-06 Chiron Corp Superoxiddismutase und deren Expression in Mikroorganismen
PH22337A (en) * 1983-11-21 1988-08-12 Ciba Geigy Ag Synthesis of fibrinolytic agents by yeast
DE3342139A1 (de) * 1983-11-22 1985-05-30 Ciba-Geigy Ag, Basel Desulfatohirudine, verfahren zu ihrer herstellung und pharmazeutische mittel
CA1341414C (fr) * 1984-03-27 2002-12-31 Paul Tolstoshev Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
ATE64956T1 (de) * 1984-06-14 1991-07-15 Ciba Geigy Ag Verfahren zur herstellung von thrombininhibitoren.
FR2568891B1 (fr) * 1984-08-09 1988-05-27 Transgene Sa Procede de preparation d'une souche, notamment de levure, transformee par un vecteur d'expression, qui peut etre cultivee sur un milieu complet sans pression de selection et souche ainsi obtenue
DE3429430A1 (de) * 1984-08-10 1986-02-20 Hoechst Ag, 6230 Frankfurt Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
FR2593518B1 (fr) * 1985-05-02 1989-09-08 Transgene Sa Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
GB8521496D0 (en) * 1985-08-29 1985-10-02 Ciba Geigy Ag Repressible yeast promoters
MC1834A1 (fr) * 1986-07-10 1988-06-03 Transgene Sa Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation

Also Published As

Publication number Publication date
ATE79403T1 (de) 1992-08-15
NO865007D0 (no) 1986-12-11
CA1341496C (en) 2006-02-14
DK595286D0 (da) 1986-12-11
FI94773B (fi) 1995-07-14
GR3006248T3 (da) 1993-06-21
NO865007L (no) 1987-06-15
IE863244L (en) 1987-06-12
NO178035B (no) 1995-10-02
IL80932A0 (en) 1987-03-31
DK175492B1 (da) 2004-11-08
AU6650486A (en) 1987-06-18
EP0225633B1 (en) 1992-08-12
IE57859B1 (en) 1993-04-21
JPH0787791B2 (ja) 1995-09-27
IL80932A (en) 1998-02-22
FI865003A0 (fi) 1986-12-08
JPS62208296A (ja) 1987-09-12
MY101203A (en) 1991-08-17
AU615413B2 (en) 1991-10-03
JPH0650990B2 (ja) 1994-07-06
PH31277A (en) 1998-07-06
DE3686399T2 (de) 1993-01-14
NO178035C (no) 1996-01-10
PT83898A (en) 1987-01-01
DE3686399D1 (de) 1992-09-17
FI94773C (fi) 1995-10-25
FI865003A (fi) 1987-06-13
HUT42523A (en) 1987-07-28
EP0225633A2 (en) 1987-06-16
ES2051688T3 (es) 1994-07-01
EP0225633A3 (en) 1988-11-30
HU209146B (en) 1994-03-28
PT83898B (pt) 1989-06-30
JPH06197766A (ja) 1994-07-19

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