DK582481A - Chimaerisk polypeptid,fremgangsmaade til dets fremstilling,kloningsmedium og mikrobielle transformanter til anvendelse ved fremgangsmaaden samt fremgangsmaade til fremstilling af humant insulin - Google Patents

Chimaerisk polypeptid,fremgangsmaade til dets fremstilling,kloningsmedium og mikrobielle transformanter til anvendelse ved fremgangsmaaden samt fremgangsmaade til fremstilling af humant insulin

Info

Publication number
DK582481A
DK582481A DK582481A DK582481A DK582481A DK 582481 A DK582481 A DK 582481A DK 582481 A DK582481 A DK 582481A DK 582481 A DK582481 A DK 582481A DK 582481 A DK582481 A DK 582481A
Authority
DK
Denmark
Prior art keywords
procedure
manufacturing
transformers
microbial
human insulin
Prior art date
Application number
DK582481A
Other languages
Danish (da)
English (en)
Inventor
D V Goeddel
K Itakura
D G Kleid
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of DK582481A publication Critical patent/DK582481A/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
DK582481A 1981-01-02 1981-12-30 Chimaerisk polypeptid,fremgangsmaade til dets fremstilling,kloningsmedium og mikrobielle transformanter til anvendelse ved fremgangsmaaden samt fremgangsmaade til fremstilling af humant insulin DK582481A (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US22204481A 1981-01-02 1981-01-02

Publications (1)

Publication Number Publication Date
DK582481A true DK582481A (da) 1982-08-13

Family

ID=22830549

Family Applications (1)

Application Number Title Priority Date Filing Date
DK582481A DK582481A (da) 1981-01-02 1981-12-30 Chimaerisk polypeptid,fremgangsmaade til dets fremstilling,kloningsmedium og mikrobielle transformanter til anvendelse ved fremgangsmaaden samt fremgangsmaade til fremstilling af humant insulin

Country Status (12)

Country Link
EP (1) EP0055945A3 (https=)
JP (1) JPS57163352A (https=)
KR (1) KR830007828A (https=)
AU (1) AU7914581A (https=)
DK (1) DK582481A (https=)
ES (1) ES8401777A1 (https=)
FI (1) FI814172L (https=)
GR (1) GR78327B (https=)
IL (1) IL64634A (https=)
NZ (1) NZ199391A (https=)
PH (1) PH20660A (https=)
ZA (1) ZA818953B (https=)

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US4758512A (en) * 1984-03-06 1988-07-19 President And Fellows Of Harvard College Hosts and methods for producing recombinant products in high yields
DK58285D0 (da) * 1984-05-30 1985-02-08 Novo Industri As Peptider samt fremstilling og anvendelse deraf
DK129385A (da) * 1985-03-22 1986-09-23 Novo Industri As Peptider og fremstilling deraf
DK437786D0 (da) * 1986-09-12 1986-09-12 Nordisk Gentofte Insulinprecursorer
DE3716722A1 (de) * 1987-05-19 1988-12-01 Hoechst Ag Gentechnologisches verfahren zur herstellung von angiogeninen
US6875589B1 (en) 1988-06-23 2005-04-05 Hoechst Aktiengesellschaft Mini-proinsulin, its preparation and use
US5716927A (en) * 1988-12-23 1998-02-10 Novo Nordisk A/S Insulin analogs having a modified B-chain
US5514646A (en) * 1989-02-09 1996-05-07 Chance; Ronald E. Insulin analogs modified at position 29 of the B chain
CA2051375C (en) * 1989-05-04 2000-09-12 Dennis J. Henner Process and compositions for the isolation of human relaxin
US5126249A (en) * 1989-05-09 1992-06-30 Eli Lilly And Company Enzymatic removal of a protein amino-terminal sequence
ATE148710T1 (de) 1992-12-02 1997-02-15 Hoechst Ag Verfahren zur gewinnung von proinsulin mit korrekt verbundenen cystinbrücken
US6001604A (en) * 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
KR0150565B1 (ko) * 1995-02-15 1998-08-17 김정재 유전자 조환에 의한 사람 인슐린 전구체의 제조 및 이를 이용한 인슐린의 제조방법
US6068993A (en) * 1997-07-02 2000-05-30 Biobras Sa Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
DE19735711C2 (de) * 1997-08-18 2001-04-26 Aventis Pharma Gmbh Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken
KR100253916B1 (ko) * 1997-12-29 2000-05-01 김충환 사람 인슐린 전구체의 제조방법
DE69840035D1 (de) 1998-03-31 2008-10-30 Tonghua Gantech Biotechnology Chimäres protein welches eine intramolekulare chap zur insulinproduktion
DE19838097A1 (de) 1998-08-24 2000-03-02 Hoechst Marion Roussel De Gmbh Verfahren zur chromatographischen Reinigung von Insulinen
RU2144957C1 (ru) * 1999-02-26 2000-01-27 Институт биоорганической химии им.М.М.Шемякина и Ю.А.Овчинникова РАН РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pPINS07, КОДИРУЮЩАЯ ГИБРИДНЫЙ ПОЛИПЕПТИД, СОДЕРЖАЩИЙ ПРОИНСУЛИН ЧЕЛОВЕКА, И ШТАММ БАКТЕРИЙ Escherichia coli - ПРОДУЦЕНТ ГИБРИДНОГО ПОЛИПЕПТИДА, СОДЕРЖАЩЕГО ПРОИНСУЛИН ЧЕЛОВЕКА
JP3406244B2 (ja) 1999-04-30 2003-05-12 伊藤ハム株式会社 新規な融合蛋白質からの組み換えインスリンの製造方法
US6521738B2 (en) 1999-12-29 2003-02-18 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogs
US6777207B2 (en) 1999-12-29 2004-08-17 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
US7378390B2 (en) 1999-12-29 2008-05-27 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
JP4795599B2 (ja) * 1999-12-29 2011-10-19 ノボ ノルディスク アクティーゼルスカブ 酵母において改良された発酵収率を有するインスリン前駆体及びインスリン前駆体類似体の製造方法
DE60120372T2 (de) 2000-03-24 2007-07-05 Genentech Inc., San Francisco Verwendung von insulin zur behandlung von knorpelkrankheiten
US6821755B2 (en) * 2000-07-27 2004-11-23 Boehringer Ingelheim International Gmbh Preparation of a recombinant protein in a prokaryotic host cell
AR025646A1 (es) 2000-09-13 2002-12-04 Beta Lab Sa Cepa de levaduras metilotroficas recombinantes productoras de un precursor de insulina, construcciones de adn y metodo para obtener la cepa.
KR100449454B1 (ko) * 2000-10-02 2004-09-21 이현철 단일사슬 인슐린 유도체의 유전자를 포함하는 당뇨병치료용 벡터
WO2002079251A2 (en) * 2001-04-02 2002-10-10 Novo Nordisk A/S Method for making human insulin precursors
US7105314B2 (en) 2001-04-02 2006-09-12 Novo Nordisk A/S Method for making human insulin precursors
CA2514416C (en) 2003-01-31 2012-08-14 Akzo Nobel N.V. Method for protein isolation in anoxic conditions
MX2008000985A (es) 2005-07-21 2008-04-07 Abbott Lab Expresion de gen multiple que incluye construcciones y metodos sorf (cuadro de lectura abierta simple) con poliproteinas, pro-proteinas y proteolisis.
AU2006291780B2 (en) 2005-09-14 2011-12-08 F. Hoffmann-La Roche Ag Cleavage of precursors of insulins by a variant of trypsin
RU2524150C2 (ru) 2006-09-22 2014-07-27 Ново Нордиск А/С Аналоги инсулина, устойчивые к протеазам
CN101173006B (zh) * 2006-10-30 2011-12-21 江苏万邦生化医药股份有限公司 一种重组人胰岛素的制备方法
US10100098B2 (en) 2006-12-13 2018-10-16 Stelis Biopharma Private Limited Insulin production methods and proinsulin constructs
US7790677B2 (en) 2006-12-13 2010-09-07 Elona Biotechnologies Insulin production methods and pro-insulin constructs
EP2201120B1 (en) 2007-09-21 2013-12-25 Cadila Healthcare Limited Gcsf fusion protein systems suitable for high expression of peptides
KR101775000B1 (ko) 2009-06-26 2017-09-04 노보 노르디스크 에이/에스 인슐린, 니코틴아미드 및 아미노산을 포함하는 제제
WO2012080320A1 (en) 2010-12-14 2012-06-21 Novo Nordisk A/S Fast-acting insulin in combination with long-acting insulin
KR20140030125A (ko) 2010-12-14 2014-03-11 노보 노르디스크 에이/에스 인슐린, 니코틴아미드 및 아미노산을 포함하는 제제
WO2012152439A1 (en) 2011-05-10 2012-11-15 Glucometrix Ag Comprehensive buffer system for renaturing human proinsulin or proinsulin derivatives
WO2013186138A1 (en) 2012-06-14 2013-12-19 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and arginine
JP2016183118A (ja) * 2015-03-25 2016-10-20 東ソー株式会社 増殖因子前駆体のリフォールディング方法
EP3344651B1 (en) 2015-09-02 2022-03-02 Merck Sharp & Dohme Corp. A process for obtaining insulin with correctly formed disulfide bonds
KR20190036956A (ko) 2017-09-28 2019-04-05 한미약품 주식회사 지속형 단쇄 인슐린 아날로그 및 이의 결합체
EP3845240B1 (en) 2018-09-12 2024-11-06 Amphastar Nanjing Pharmaceuticals, Inc. Pro-insulin aspart structure and method for preparing insulin aspart
CN118754967A (zh) 2019-12-30 2024-10-11 甘李药业股份有限公司 胰岛素衍生物
EP4086277A4 (en) 2019-12-30 2024-02-14 Gan & Lee Pharmaceuticals Co., Ltd. Long-acting glp-1 compound
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Also Published As

Publication number Publication date
FI814172A7 (fi) 1982-07-03
EP0055945A3 (en) 1982-09-15
JPS57163352A (en) 1982-10-07
NZ199391A (en) 1985-12-13
PH20660A (en) 1987-03-23
IL64634A (en) 1986-03-31
FI814172L (fi) 1982-07-03
GR78327B (https=) 1984-09-26
AU7914581A (en) 1982-07-08
KR830007828A (ko) 1983-11-07
IL64634A0 (en) 1982-03-31
ES508446A0 (es) 1984-01-01
ES8401777A1 (es) 1984-01-01
EP0055945A2 (en) 1982-07-14
ZA818953B (en) 1983-02-23

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