DK3690060T3 - Method for identification and relative quantification of nucleic acid sequence expression, splice variant, translocation, copy number, or methylation changes using combined nuclease, ligation, and polymerase reactions with carryover prevention - Google Patents

Method for identification and relative quantification of nucleic acid sequence expression, splice variant, translocation, copy number, or methylation changes using combined nuclease, ligation, and polymerase reactions with carryover prevention Download PDF

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Publication number
DK3690060T3
DK3690060T3 DK20150354.7T DK20150354T DK3690060T3 DK 3690060 T3 DK3690060 T3 DK 3690060T3 DK 20150354 T DK20150354 T DK 20150354T DK 3690060 T3 DK3690060 T3 DK 3690060T3
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translocation
ligation
car
identification
nucleic acid
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DK20150354.7T
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English (en)
Inventor
Francis Barany
John William Efcavitch
Rueda Cristian Ruiz
Jianmin Huang
Philip B Feinberg
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Univ Cornell
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/331—Methylation site specific nuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/118—Prognosis of disease development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
DK20150354.7T 2014-10-08 2015-10-08 Method for identification and relative quantification of nucleic acid sequence expression, splice variant, translocation, copy number, or methylation changes using combined nuclease, ligation, and polymerase reactions with carryover prevention DK3690060T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462061376P 2014-10-08 2014-10-08
US201562103894P 2015-01-15 2015-01-15
EP15849301.5A EP3204520B1 (en) 2014-10-08 2015-10-08 Method for identification and quantification of nucleic acid expression, splice variant, translocation, copy number, or methylation changes

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DK3690060T3 true DK3690060T3 (da) 2022-01-17

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DK15849301.5T DK3204520T3 (da) 2014-10-08 2015-10-08 Fremgangsmåde til identificering og kvantificering af nukleinsyreekspression, splejsningsvariant, translokation, kopiantal eller methyleringsændringer
DK20150354.7T DK3690060T3 (da) 2014-10-08 2015-10-08 Method for identification and relative quantification of nucleic acid sequence expression, splice variant, translocation, copy number, or methylation changes using combined nuclease, ligation, and polymerase reactions with carryover prevention

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DK15849301.5T DK3204520T3 (da) 2014-10-08 2015-10-08 Fremgangsmåde til identificering og kvantificering af nukleinsyreekspression, splejsningsvariant, translokation, kopiantal eller methyleringsændringer

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US (2) US10344321B2 (da)
EP (3) EP3690060B1 (da)
JP (3) JP6871160B2 (da)
CN (2) CN113481281A (da)
AU (2) AU2015330844B2 (da)
BR (1) BR112017007033B1 (da)
CA (2) CA3187713A1 (da)
DK (2) DK3204520T3 (da)
ES (2) ES2784342T3 (da)
IL (3) IL296204B1 (da)
PT (2) PT3204520T (da)
SG (2) SG10202008961UA (da)
WO (1) WO2016057832A2 (da)

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Also Published As

Publication number Publication date
EP3204520B1 (en) 2020-02-12
BR112017007033B1 (pt) 2023-10-31
CA2963687A1 (en) 2016-04-14
US20180265917A1 (en) 2018-09-20
IL251513A0 (en) 2017-05-29
AU2015330844A1 (en) 2017-04-27
EP3204520A4 (en) 2018-03-07
CN113481281A (zh) 2021-10-08
IL296204A (en) 2022-11-01
SG10202008961UA (en) 2020-10-29
JP2021106608A (ja) 2021-07-29
IL286496A (en) 2021-10-31
EP3690060B1 (en) 2021-12-08
CN107002145A (zh) 2017-08-01
EP3690060A1 (en) 2020-08-05
ES2904909T3 (es) 2022-04-06
US11466311B2 (en) 2022-10-11
WO2016057832A3 (en) 2016-06-16
US10344321B2 (en) 2019-07-09
CA3187713A1 (en) 2016-04-14
AU2022200229A1 (en) 2022-02-10
DK3204520T3 (da) 2020-04-20
ES2784342T3 (es) 2020-09-24
PT3690060T (pt) 2022-01-27
JP2023040174A (ja) 2023-03-22
BR112017007033A2 (pt) 2017-12-12
JP7209762B2 (ja) 2023-01-20
JP6871160B2 (ja) 2021-05-12
US20190316187A1 (en) 2019-10-17
CA2963687C (en) 2023-03-28
JP2017533697A (ja) 2017-11-16
EP3204520A2 (en) 2017-08-16
IL296204B1 (en) 2024-04-01
CN107002145B (zh) 2021-07-30
WO2016057832A2 (en) 2016-04-14
AU2015330844B2 (en) 2022-02-03
PT3204520T (pt) 2020-04-16
EP4026914A1 (en) 2022-07-13
IL251513B (en) 2021-10-31
SG11201702831XA (en) 2017-05-30

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