DK3684930T3 - Modificering af ikke-kodende plante-RNA-molekylers specificitet for inaktivering af genekspression - Google Patents

Modificering af ikke-kodende plante-RNA-molekylers specificitet for inaktivering af genekspression Download PDF

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Publication number
DK3684930T3
DK3684930T3 DK18786034.1T DK18786034T DK3684930T3 DK 3684930 T3 DK3684930 T3 DK 3684930T3 DK 18786034 T DK18786034 T DK 18786034T DK 3684930 T3 DK3684930 T3 DK 3684930T3
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Denmark
Prior art keywords
inactivation
specificity
modification
gene expression
rna molecules
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DK18786034.1T
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Danish (da)
English (en)
Inventor
Eyal Maori
Yaron Galanty
Cristina Pignocchi
Garcia Angela Chaparro
Ofir Meir
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Tropic Biosciences Uk Ltd
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Priority claimed from GBGB1715116.8A external-priority patent/GB201715116D0/en
Priority claimed from GBGB1715113.5A external-priority patent/GB201715113D0/en
Priority claimed from GBGB1719516.5A external-priority patent/GB201719516D0/en
Application filed by Tropic Biosciences Uk Ltd filed Critical Tropic Biosciences Uk Ltd
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    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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DK18786034.1T 2017-09-19 2018-09-18 Modificering af ikke-kodende plante-RNA-molekylers specificitet for inaktivering af genekspression DK3684930T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB1715116.8A GB201715116D0 (en) 2017-09-19 2017-09-19 Modifying the specificity of non-coding RNA molecules for silencing of target RNA in eukaryotic cells
GBGB1715113.5A GB201715113D0 (en) 2017-09-19 2017-09-19 Modifying the specificity of plant non-coding RNA molecules for silencing of target RNA
GBGB1719516.5A GB201719516D0 (en) 2017-11-23 2017-11-23 Modifying the specificity of plant non-coding RNA molecules for silencing of target RNA
PCT/IB2018/057160 WO2019058255A1 (en) 2017-09-19 2018-09-18 MODIFICATION OF THE SPECIFICITY OF VEGETABLE NON-CODANT RNA MOLECULES FOR THE SILENCING OF GENE EXPRESSION

Publications (1)

Publication Number Publication Date
DK3684930T3 true DK3684930T3 (da) 2022-06-07

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DK18786034.1T DK3684930T3 (da) 2017-09-19 2018-09-18 Modificering af ikke-kodende plante-RNA-molekylers specificitet for inaktivering af genekspression

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Country Link
US (5) US20200270604A1 (enExample)
EP (3) EP3684930B1 (enExample)
JP (3) JP7422069B2 (enExample)
KR (4) KR20200051808A (enExample)
CN (4) CN111448318B (enExample)
AU (5) AU2018336128B2 (enExample)
BR (2) BR112020005416A2 (enExample)
CA (2) CA3074948A1 (enExample)
DK (1) DK3684930T3 (enExample)
ES (1) ES2915576T3 (enExample)
IL (3) IL313387A (enExample)
SG (2) SG11202002481RA (enExample)
WO (2) WO2019058253A1 (enExample)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202002481RA (en) 2017-09-19 2020-04-29 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells
EP3794130A4 (en) 2018-05-16 2022-07-27 Synthego Corporation METHODS AND SYSTEMS FOR DESIGNING AND USING GUIDE RNA
CN110857438B (zh) * 2018-08-20 2022-05-17 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 一种高效产生siRNA的烟草花叶病毒基因片段、弱毒疫苗、制备方法及其应用
WO2020178099A1 (en) 2019-03-01 2020-09-10 Syngenta Crop Protection Ag Suppression of target gene expression through genome editing of native mirnas
GB201903519D0 (en) * 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest
GB201903521D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd No title
GB201903520D0 (en) * 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing genes in eukaryotic cells
CN110551719B (zh) * 2019-07-30 2023-06-02 中山大学 长链非编码rna基因alex1及其在提高水稻白叶枯病抗性中的应用
CN111508558B (zh) * 2020-03-23 2021-12-14 广州赛业百沐生物科技有限公司 一种基于CRISPR-Cas9技术设计点突变模型的方法及系统
CN111676220B (zh) * 2020-05-21 2022-03-29 扬州大学 杨树长链非编码RNA lnc11及其应用
WO2022006310A2 (en) * 2020-06-30 2022-01-06 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions for genome editing and methods of use thereof
CN114073760B (zh) * 2020-08-21 2024-02-13 北京大学 Rdr蛋白在肿瘤治疗中的应用
US20240000970A1 (en) * 2020-12-01 2024-01-04 Emendobio Inc. Differential knockout of a heterozygous allele of lrrk2
CN112359049B (zh) * 2020-12-10 2022-01-28 昆明理工大学 一种岷江百合几丁质酶基因LrCHI2及其应用
CN112795568B (zh) * 2021-01-06 2023-07-18 河南农业大学 一种控制玉米雌穗穗粗杂种优势的非编码基因的应用及其前体序列
GB202103256D0 (en) 2021-03-09 2021-04-21 Tropic Biosciences Uk Ltd Method for silencing genes
CN114134155B (zh) * 2021-06-29 2023-09-12 中国农业科学院油料作物研究所 一种mlo基因突变体及其制备方法与应用
CN113444727B (zh) * 2021-06-30 2022-06-21 中国热带农业科学院热带生物技术研究所 一种LncRNA及其应用
US20240287535A1 (en) 2021-07-02 2024-08-29 Tropic Biosciences UK Limited Delay or prevention of browning in banana fruit
KR102573952B1 (ko) * 2021-08-09 2023-09-01 경상국립대학교산학협력단 E2와 이의 상동체 동시 타겟 유전자교정 시스템 및 이의 용도
KR102573947B1 (ko) * 2021-08-09 2023-09-01 경상국립대학교산학협력단 콩 유전자교정 효율 증대를 위한 유전자교정 시스템 및 이의 용도
KR102573948B1 (ko) * 2021-08-09 2023-09-01 경상국립대학교산학협력단 Mips1과 이의 상동체 동시 타겟 유전자교정 시스템 및 이의 용도
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CN115725656B (zh) * 2021-09-01 2026-01-16 联邦生物科技(珠海横琴)有限公司 一种以细胞质rna病毒为载体递送干扰rna的方法
GB202112865D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to black sigatoka disease in banana
GB202112866D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to fusarium wilt in a banana
CN114807128B (zh) * 2022-03-24 2023-09-01 四川农业大学 一种lncRNA-BTRL及其应用
CN114794029B (zh) * 2022-06-09 2023-02-28 上海大学 一种计数秀丽隐杆线虫子宫内发育胚胎数目的方法
CN116064644B (zh) * 2022-10-10 2023-10-10 中国农业科学院生物技术研究所 SbAGO1b蛋白及其编码基因在调控植物抗虫性中的应用
CN116103334A (zh) * 2022-11-16 2023-05-12 西北农林科技大学 一种猕猴桃抗溃疡病基因功能筛选专用瞬时转化体系
CN115976050B (zh) * 2022-11-16 2024-06-18 西北农林科技大学 猕猴桃感病基因及其适用的vigs沉默体系构建
CN120898000A (zh) 2023-01-13 2025-11-04 拉维洛克治疗有限责任公司 新的多核苷酸、细胞和方法
GB202305021D0 (en) 2023-04-04 2023-05-17 Tropic Biosciences Uk Ltd Methods for generating breaks in a genome
CN120283056A (zh) * 2023-10-30 2025-07-08 上海交通大学 一种靶向抑制基因表达的方法及应用
CN118581143B (zh) * 2024-08-02 2024-10-18 内蒙古农业大学 一个长链非编码RNA基因StlncFAD-AS在马铃薯抗病育种中的应用

Family Cites Families (117)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
NL154598B (nl) 1970-11-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking.
NL154599B (nl) 1970-12-28 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking.
US3901654A (en) 1971-06-21 1975-08-26 Biological Developments Receptor assays of biologically active compounds employing biologically specific receptors
US3853987A (en) 1971-09-01 1974-12-10 W Dreyer Immunological reagent and radioimmuno assay
US3867517A (en) 1971-12-21 1975-02-18 Abbott Lab Direct radioimmunoassay for antigens and their antibodies
NL171930C (nl) 1972-05-11 1983-06-01 Akzo Nv Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen.
US3850578A (en) 1973-03-12 1974-11-26 H Mcconnell Process for assaying for biologically active molecules
US3935074A (en) 1973-12-17 1976-01-27 Syva Company Antibody steric hindrance immunoassay with two antibodies
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
US4034074A (en) 1974-09-19 1977-07-05 The Board Of Trustees Of Leland Stanford Junior University Universal reagent 2-site immunoradiometric assay using labelled anti (IgG)
US3984533A (en) 1975-11-13 1976-10-05 General Electric Company Electrophoretic method of detecting antigen-antibody reaction
US4098876A (en) 1976-10-26 1978-07-04 Corning Glass Works Reverse sandwich immunoassay
US4879219A (en) 1980-09-19 1989-11-07 General Hospital Corporation Immunoassay utilizing monoclonal high affinity IgM antibodies
CA1192510A (en) 1981-05-27 1985-08-27 Lawrence E. Pelcher Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom
JPS6054684A (ja) 1983-09-05 1985-03-29 Teijin Ltd 新規ｄνａ及びハイブリツドｄνａ
US5011771A (en) 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
US4666828A (en) 1984-08-15 1987-05-19 The General Hospital Corporation Test for Huntington's disease
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
CA1288073C (en) 1985-03-07 1991-08-27 Paul G. Ahlquist Rna transformation vector
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4801531A (en) 1985-04-17 1989-01-31 Biotechnology Research Partners, Ltd. Apo AI/CIII genomic polymorphisms predictive of atherosclerosis
ATE141646T1 (de) 1986-04-09 1996-09-15 Genzyme Corp Genetisch transformierte tiere, die ein gewünschtes protein in milch absondern
GB8608850D0 (en) 1986-04-11 1986-05-14 Diatech Ltd Packaging system
JPS6314693A (ja) 1986-07-04 1988-01-21 Sumitomo Chem Co Ltd 植物ウイルスｒｎａベクタ−
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
DE3784860D1 (de) 1986-12-05 1993-04-22 Ciba Geigy Ag Verbessertes verfahren zur transformation von pflanzlichen protoplasten.
ATE108828T1 (de) 1987-02-09 1994-08-15 Lubrizol Genetics Inc Hybrides rns-virus.
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US5693507A (en) 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5272057A (en) 1988-10-14 1993-12-21 Georgetown University Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase
US5302523A (en) 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5192659A (en) 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
US5484956A (en) 1990-01-22 1996-01-16 Dekalb Genetics Corporation Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin
JP3209744B2 (ja) 1990-01-22 2001-09-17 デカルブ・ジェネティクス・コーポレーション 結実能力のある遺伝子変換コーン
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
US5486359A (en) 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
US5384253A (en) 1990-12-28 1995-01-24 Dekalb Genetics Corporation Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes
DE69334225D1 (de) 1992-07-07 2008-07-31 Japan Tobacco Inc Verfahren zur transformation einer monokotyledon pflanze
US5281521A (en) 1992-07-20 1994-01-25 The Trustees Of The University Of Pennsylvania Modified avidin-biotin technique
WO1994002620A2 (en) 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
US6329191B1 (en) 1993-08-30 2001-12-11 Hawaii Biotechnology Group, Inc. DNA encoding recombinant coffee bean alpha-galactosidase
US5998193A (en) 1994-06-24 1999-12-07 Gene Shears Pty., Ltd. Ribozymes with optimized hybridizing arms, stems, and loops, tRNA embedded ribozymes and compositions thereof
US5693512A (en) 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
US6075184A (en) 1996-03-26 2000-06-13 University Of Hawaii Purified proteins, recombinant DNA sequences and processes for producing caffeine free beverages
US5886164A (en) 1996-04-15 1999-03-23 Zeneca Limited DNA encoding enzymes related to ethylene biosynthesis and ripening from banana
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
WO1998042848A1 (en) 1997-03-24 1998-10-01 University Of Hawaii Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages
AU6324798A (en) 1998-02-13 1998-09-08 University Of Hawaii Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages
CA2359868A1 (en) 1999-01-14 2000-07-20 Monsanto Company Soybean transformation method
EP1020519A1 (en) 1999-01-15 2000-07-19 Introgene B.V. Minor histocompatibility antigens and their use in the diagnosis and treatment of tumors
JP3520328B2 (ja) 2000-10-06 2004-04-19 奈良先端科学技術大学院大学長 コーヒー属植物のテオブロミン合成酵素ポリペプチド及び当該ポリペプチドをコードする遺伝子
ATE318319T1 (de) 2001-10-10 2006-03-15 Nestle Sa Kaffeepflanze mit verringerter alpha-d- galaktosidase-aktivität
JP2004049022A (ja) 2002-07-16 2004-02-19 Nara Institute Of Science & Technology 遺伝子組換えによるカフェインレスコーヒー植物の製造方法
US9567591B2 (en) 2003-05-15 2017-02-14 Mello Biotechnology, Inc. Generation of human embryonic stem-like cells using intronic RNA
WO2005086896A2 (en) * 2004-03-10 2005-09-22 Ulrich Thomann Delivery vectors for short interfering rna, micro-rna and antisense rna
WO2005111211A2 (en) 2004-05-14 2005-11-24 Rosetta Genomics Ltd. Micronas and uses thereof
WO2006040763A2 (en) 2004-10-12 2006-04-20 Technion Research & Development Foundation Ltd. Isolated primate embryonic cells and methods of generating and using same
CA2625928A1 (en) 2005-10-14 2007-04-26 James Gerard Mccarthy Nucleic acids and proteins associated with galactomannan synthesis in coffee
EP2650366B1 (en) 2005-10-18 2017-03-22 Precision Biosciences Rationally designed meganucleases with altered sequence specificity and DNA-binding affinity
EP1945765A2 (en) 2005-10-28 2008-07-23 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Structures of active guide rna molecules and method of selection
WO2008148223A1 (en) 2007-06-07 2008-12-11 Agriculture And Agri-Food Canada Nanocarrier based plant transfection and transduction
BRPI0817911B8 (pt) 2007-10-05 2022-06-28 Dow Agrosciences Llc Processos para transferência de substâncias moleculares para dentro de células vegetais e processo para expressão de um gene
US8212019B2 (en) * 2008-07-30 2012-07-03 University Of Massachusetts Nucleic acid silencing sequences
US20100293671A1 (en) 2009-05-13 2010-11-18 National Taiwan University Composition and Method for Prolonging the Shelf Life of Banana by Using Interfering RNA
EP2292176B1 (en) 2009-09-07 2019-01-09 Nobel Biocare Services AG Implantation set
CA2819423C (en) * 2010-12-02 2020-12-22 Keygene N.V. Targeted alteration of dna
CA2899650A1 (en) 2012-02-29 2013-09-06 Benitec Biopharma Limited Pain treatment
WO2013184768A1 (en) 2012-06-05 2013-12-12 University Of Georgia Research Foundation, Inc. Compositions and methods of gene silencing in plants
AR092482A1 (es) 2012-09-07 2015-04-22 Dow Agrosciences Llc Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas
US20150291967A1 (en) 2012-10-31 2015-10-15 Luc Mathis Coupling herbicide resistance with targeted insertion of transgenes in plants
EP2931026B1 (en) 2012-11-21 2025-05-07 Nunhems B.V. Solanum lycopersicum plants having non-transgenic alterations in the acs2 gene
JP2016507514A (ja) 2013-01-08 2016-03-10 ベニテック バイオファーマ リミテッド 加齢黄斑変性の処置
US20150056629A1 (en) 2013-04-14 2015-02-26 Katriona Guthrie-Honea Compositions, systems, and methods for detecting a DNA sequence
WO2014186686A2 (en) 2013-05-17 2014-11-20 Two Blades Foundation Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases
ES2670531T3 (es) 2013-05-29 2018-05-30 Cellectis S.A. Un método para producir una escisión de ADN precisa utilizando la actividad nickasa de Cas9
WO2014194190A1 (en) 2013-05-30 2014-12-04 The Penn State Research Foundation Gene targeting and genetic modification of plants via rna-guided genome editing
EP3725885A1 (en) 2013-06-17 2020-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof
EP3178928B1 (en) 2013-08-07 2020-06-10 Regeneron Pharmaceuticals, Inc. Pint knockout mouse exhibiting a premature aging-associated phenotype
CA3221516A1 (en) 2013-08-22 2015-02-26 E. I. Du Pont De Nemours And Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
RU2016128077A (ru) * 2013-12-12 2018-12-06 Те Брод Инститьют Инк. Доставка, применение и применения в терапии систем и композиций crispr-cas для лечения обусловленных hbv и вирусных заболеваний и нарушений
BR102013032317B1 (pt) 2013-12-16 2022-04-19 Embrapa - Empresa Brasileira De Pesquisa Agropecuária Método, kit e oligonucleotídeos para identificação de plantas com teor de cafeína reduzido, método de gerar plantas com teor de cafeína reduzido, planta com teor reduzido de cafeína
EP4063503A1 (en) 2014-02-11 2022-09-28 The Regents of the University of Colorado, a body corporate Crispr enabled multiplexed genome engineering
CA2956545A1 (en) 2014-08-04 2016-02-11 Fred Hutchinson Cancer Research Center T cell immunotherapy specific for wt-1
US20160076093A1 (en) 2014-08-04 2016-03-17 University Of Washington Multiplex homology-directed repair
US20170354745A1 (en) 2014-10-09 2017-12-14 The Regents Of The University Of California Targeted disruption of a csf1-dap12 pathway member gene for the treatment of neuropathic pain
WO2016089433A1 (en) 2014-12-03 2016-06-09 Agilent Technologies, Inc. Guide rna with chemical modifications
US20170327834A1 (en) * 2014-12-15 2017-11-16 Syngenta Participations Ag Pesticidal microrna carriers and use thereof
CA2972454C (en) 2014-12-31 2024-09-10 Phykion Inc. Compositions and methods for high efficiency in in vivo genome editing
US20180073035A1 (en) 2015-01-19 2018-03-15 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences A method for precise modification of plant via transient gene expression
EP4223786A3 (en) 2015-03-06 2023-08-16 Public University Corporation Yokohama City University Novel anti-pad4 antibodies
EP3303585A4 (en) 2015-06-03 2018-10-31 Board of Regents of the University of Nebraska Dna editing using single-stranded dna
IL293323B2 (en) 2015-06-18 2024-01-01 Massachusetts Inst Technology Crispr enzyme mutations reducing off-target effects
CN106701752B (zh) 2015-09-01 2019-07-12 清华大学 人工合成的microRNA簇及其构建方法与所用合成microRNA单元
US12049625B2 (en) 2016-01-14 2024-07-30 The Brigham And Women's Hospital, Inc. Genome editing for treating glioblastoma
CN105647962A (zh) 2016-02-15 2016-06-08 浙江大学 运用CRISPR-Cas9系统敲除水稻MIRNA393b茎环序列的基因编辑方法
CN105821075B (zh) 2016-04-22 2017-09-12 湖南农业大学 一种茶树咖啡因合成酶CRISPR/Cas9基因组编辑载体的构建方法
CN106367435B (zh) 2016-09-07 2019-11-08 电子科技大学 一种水稻miRNA定向敲除的方法
AU2018225825A1 (en) 2017-02-22 2019-09-12 Université de Montréal Novel minor histocompatibility antigens and uses thereof
WO2018178987A1 (en) 2017-03-30 2018-10-04 Ramot At Tel-Aviv University Ltd. Methods of treating alzheimer's disease
EP3601579B1 (en) 2017-03-31 2023-10-04 Pioneer Hi-Bred International, Inc. Expression modulating elements and use thereof
GB201708662D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing shelf-life of banana
WO2018220582A1 (en) 2017-05-31 2018-12-06 Tropic Biosciences UK Limited Methods of selecting cells comprising genome editing events
GB201708665D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing extractability of solids from coffee beans
CA3064601A1 (en) 2017-06-26 2019-01-03 President And Fellows Of Harvard College Crispr/cas-adenine deaminase based compositions, systems, and methods for targeted nucleic acid editing
SG11202002481RA (en) 2017-09-19 2020-04-29 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells
GB201807192D0 (en) 2018-05-01 2018-06-13 Tropic Biosciences Uk Ltd Compositions and methods for reducing caffeine content in coffee beans
WO2020178099A1 (en) 2019-03-01 2020-09-10 Syngenta Crop Protection Ag Suppression of target gene expression through genome editing of native mirnas
GB201903519D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest
GB201903521D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd No title
GB201903520D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing genes in eukaryotic cells
GB202103256D0 (en) 2021-03-09 2021-04-21 Tropic Biosciences Uk Ltd Method for silencing genes

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