AU2018336128B2 - Modifying the specificity of plant non-coding RNA molecules for silencing gene expression - Google Patents
Modifying the specificity of plant non-coding RNA molecules for silencing gene expression Download PDFInfo
- Publication number
- AU2018336128B2 AU2018336128B2 AU2018336128A AU2018336128A AU2018336128B2 AU 2018336128 B2 AU2018336128 B2 AU 2018336128B2 AU 2018336128 A AU2018336128 A AU 2018336128A AU 2018336128 A AU2018336128 A AU 2018336128A AU 2018336128 B2 AU2018336128 B2 AU 2018336128B2
- Authority
- AU
- Australia
- Prior art keywords
- rna
- plant
- gene
- target
- molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8275—Glyphosate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/113—Antisense targeting other non-coding nucleic acids, e.g. antagomirs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
- C12N2310/141—MicroRNAs, miRNAs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Physiology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2023202078A AU2023202078B2 (en) | 2017-09-19 | 2023-04-04 | Modifying the specificity of plant non-coding RNA molecules for silencing gene expression |
| AU2026200565A AU2026200565A1 (en) | 2017-09-19 | 2026-01-28 | Modifying the specificity of plant non-coding RNA molecules for silencing gene expression |
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1715116.8A GB201715116D0 (en) | 2017-09-19 | 2017-09-19 | Modifying the specificity of non-coding RNA molecules for silencing of target RNA in eukaryotic cells |
| GBGB1715113.5A GB201715113D0 (en) | 2017-09-19 | 2017-09-19 | Modifying the specificity of plant non-coding RNA molecules for silencing of target RNA |
| GB1715113.5 | 2017-09-19 | ||
| GB1715116.8 | 2017-09-19 | ||
| GB1719516.5 | 2017-11-23 | ||
| GBGB1719516.5A GB201719516D0 (en) | 2017-11-23 | 2017-11-23 | Modifying the specificity of plant non-coding RNA molecules for silencing of target RNA |
| PCT/IB2018/057160 WO2019058255A1 (en) | 2017-09-19 | 2018-09-18 | MODIFICATION OF THE SPECIFICITY OF VEGETABLE NON-CODANT RNA MOLECULES FOR THE SILENCING OF GENE EXPRESSION |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2023202078A Division AU2023202078B2 (en) | 2017-09-19 | 2023-04-04 | Modifying the specificity of plant non-coding RNA molecules for silencing gene expression |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2018336128A1 AU2018336128A1 (en) | 2020-04-30 |
| AU2018336128B2 true AU2018336128B2 (en) | 2023-01-05 |
Family
ID=63840882
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2018336128A Active AU2018336128B2 (en) | 2017-09-19 | 2018-09-18 | Modifying the specificity of plant non-coding RNA molecules for silencing gene expression |
| AU2018336126A Active AU2018336126B2 (en) | 2017-09-19 | 2018-09-18 | Modifying the specificity of non-coding RNA molecules for silencing gene expression in eukaryotic cells |
| AU2023202078A Active AU2023202078B2 (en) | 2017-09-19 | 2023-04-04 | Modifying the specificity of plant non-coding RNA molecules for silencing gene expression |
| AU2025202319A Pending AU2025202319A1 (en) | 2017-09-19 | 2025-04-01 | Modifying the specificity of non-coding RNA molecules for silencing gene expression in eukaryotic cells |
| AU2026200565A Pending AU2026200565A1 (en) | 2017-09-19 | 2026-01-28 | Modifying the specificity of plant non-coding RNA molecules for silencing gene expression |
Family Applications After (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2018336126A Active AU2018336126B2 (en) | 2017-09-19 | 2018-09-18 | Modifying the specificity of non-coding RNA molecules for silencing gene expression in eukaryotic cells |
| AU2023202078A Active AU2023202078B2 (en) | 2017-09-19 | 2023-04-04 | Modifying the specificity of plant non-coding RNA molecules for silencing gene expression |
| AU2025202319A Pending AU2025202319A1 (en) | 2017-09-19 | 2025-04-01 | Modifying the specificity of non-coding RNA molecules for silencing gene expression in eukaryotic cells |
| AU2026200565A Pending AU2026200565A1 (en) | 2017-09-19 | 2026-01-28 | Modifying the specificity of plant non-coding RNA molecules for silencing gene expression |
Country Status (13)
| Country | Link |
|---|---|
| US (5) | US20200270604A1 (enExample) |
| EP (3) | EP3684930B1 (enExample) |
| JP (3) | JP7422069B2 (enExample) |
| KR (4) | KR20200051808A (enExample) |
| CN (4) | CN111448318B (enExample) |
| AU (5) | AU2018336128B2 (enExample) |
| BR (2) | BR112020005416A2 (enExample) |
| CA (2) | CA3074948A1 (enExample) |
| DK (1) | DK3684930T3 (enExample) |
| ES (1) | ES2915576T3 (enExample) |
| IL (3) | IL313387A (enExample) |
| SG (2) | SG11202002481RA (enExample) |
| WO (2) | WO2019058253A1 (enExample) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG11202002481RA (en) | 2017-09-19 | 2020-04-29 | Tropic Biosciences Uk Ltd | Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells |
| EP3794130A4 (en) | 2018-05-16 | 2022-07-27 | Synthego Corporation | METHODS AND SYSTEMS FOR DESIGNING AND USING GUIDE RNA |
| CN110857438B (zh) * | 2018-08-20 | 2022-05-17 | 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 | 一种高效产生siRNA的烟草花叶病毒基因片段、弱毒疫苗、制备方法及其应用 |
| WO2020178099A1 (en) | 2019-03-01 | 2020-09-10 | Syngenta Crop Protection Ag | Suppression of target gene expression through genome editing of native mirnas |
| GB201903519D0 (en) * | 2019-03-14 | 2019-05-01 | Tropic Biosciences Uk Ltd | Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest |
| GB201903521D0 (en) | 2019-03-14 | 2019-05-01 | Tropic Biosciences Uk Ltd | No title |
| GB201903520D0 (en) * | 2019-03-14 | 2019-05-01 | Tropic Biosciences Uk Ltd | Modifying the specificity of non-coding rna molecules for silencing genes in eukaryotic cells |
| CN110551719B (zh) * | 2019-07-30 | 2023-06-02 | 中山大学 | 长链非编码rna基因alex1及其在提高水稻白叶枯病抗性中的应用 |
| CN111508558B (zh) * | 2020-03-23 | 2021-12-14 | 广州赛业百沐生物科技有限公司 | 一种基于CRISPR-Cas9技术设计点突变模型的方法及系统 |
| CN111676220B (zh) * | 2020-05-21 | 2022-03-29 | 扬州大学 | 杨树长链非编码RNA lnc11及其应用 |
| WO2022006310A2 (en) * | 2020-06-30 | 2022-01-06 | Arizona Board Of Regents On Behalf Of The University Of Arizona | Compositions for genome editing and methods of use thereof |
| CN114073760B (zh) * | 2020-08-21 | 2024-02-13 | 北京大学 | Rdr蛋白在肿瘤治疗中的应用 |
| US20240000970A1 (en) * | 2020-12-01 | 2024-01-04 | Emendobio Inc. | Differential knockout of a heterozygous allele of lrrk2 |
| CN112359049B (zh) * | 2020-12-10 | 2022-01-28 | 昆明理工大学 | 一种岷江百合几丁质酶基因LrCHI2及其应用 |
| CN112795568B (zh) * | 2021-01-06 | 2023-07-18 | 河南农业大学 | 一种控制玉米雌穗穗粗杂种优势的非编码基因的应用及其前体序列 |
| GB202103256D0 (en) | 2021-03-09 | 2021-04-21 | Tropic Biosciences Uk Ltd | Method for silencing genes |
| CN114134155B (zh) * | 2021-06-29 | 2023-09-12 | 中国农业科学院油料作物研究所 | 一种mlo基因突变体及其制备方法与应用 |
| CN113444727B (zh) * | 2021-06-30 | 2022-06-21 | 中国热带农业科学院热带生物技术研究所 | 一种LncRNA及其应用 |
| US20240287535A1 (en) | 2021-07-02 | 2024-08-29 | Tropic Biosciences UK Limited | Delay or prevention of browning in banana fruit |
| KR102573952B1 (ko) * | 2021-08-09 | 2023-09-01 | 경상국립대학교산학협력단 | E2와 이의 상동체 동시 타겟 유전자교정 시스템 및 이의 용도 |
| KR102573947B1 (ko) * | 2021-08-09 | 2023-09-01 | 경상국립대학교산학협력단 | 콩 유전자교정 효율 증대를 위한 유전자교정 시스템 및 이의 용도 |
| KR102573948B1 (ko) * | 2021-08-09 | 2023-09-01 | 경상국립대학교산학협력단 | Mips1과 이의 상동체 동시 타겟 유전자교정 시스템 및 이의 용도 |
| KR102574819B1 (ko) * | 2021-08-09 | 2023-09-04 | 경상국립대학교산학협력단 | P34와 이의 상동체 동시 타겟 유전자교정 시스템 및 이의 용도 |
| KR102584891B1 (ko) * | 2021-08-09 | 2023-10-04 | 경상국립대학교산학협력단 | GmIPK1 유전자교정 시스템 및 이의 용도 |
| CN115725656B (zh) * | 2021-09-01 | 2026-01-16 | 联邦生物科技(珠海横琴)有限公司 | 一种以细胞质rna病毒为载体递送干扰rna的方法 |
| GB202112865D0 (en) | 2021-09-09 | 2021-10-27 | Tropic Biosciences Uk Ltd | Resistance to black sigatoka disease in banana |
| GB202112866D0 (en) | 2021-09-09 | 2021-10-27 | Tropic Biosciences Uk Ltd | Resistance to fusarium wilt in a banana |
| CN114807128B (zh) * | 2022-03-24 | 2023-09-01 | 四川农业大学 | 一种lncRNA-BTRL及其应用 |
| CN114794029B (zh) * | 2022-06-09 | 2023-02-28 | 上海大学 | 一种计数秀丽隐杆线虫子宫内发育胚胎数目的方法 |
| CN116064644B (zh) * | 2022-10-10 | 2023-10-10 | 中国农业科学院生物技术研究所 | SbAGO1b蛋白及其编码基因在调控植物抗虫性中的应用 |
| CN116103334A (zh) * | 2022-11-16 | 2023-05-12 | 西北农林科技大学 | 一种猕猴桃抗溃疡病基因功能筛选专用瞬时转化体系 |
| CN115976050B (zh) * | 2022-11-16 | 2024-06-18 | 西北农林科技大学 | 猕猴桃感病基因及其适用的vigs沉默体系构建 |
| CN120898000A (zh) | 2023-01-13 | 2025-11-04 | 拉维洛克治疗有限责任公司 | 新的多核苷酸、细胞和方法 |
| GB202305021D0 (en) | 2023-04-04 | 2023-05-17 | Tropic Biosciences Uk Ltd | Methods for generating breaks in a genome |
| CN120283056A (zh) * | 2023-10-30 | 2025-07-08 | 上海交通大学 | 一种靶向抑制基因表达的方法及应用 |
| CN118581143B (zh) * | 2024-08-02 | 2024-10-18 | 内蒙古农业大学 | 一个长链非编码RNA基因StlncFAD-AS在马铃薯抗病育种中的应用 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050203047A1 (en) * | 2004-03-10 | 2005-09-15 | Ulrich Thomann | Delivery vectors for short interfering RNA, micro-RNA and antisense RNA |
Family Cites Families (116)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL154600B (nl) | 1971-02-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen. |
| NL154598B (nl) | 1970-11-10 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van laagmoleculire verbindingen en van eiwitten die deze verbindingen specifiek kunnen binden, alsmede testverpakking. |
| NL154599B (nl) | 1970-12-28 | 1977-09-15 | Organon Nv | Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen, alsmede testverpakking. |
| US3901654A (en) | 1971-06-21 | 1975-08-26 | Biological Developments | Receptor assays of biologically active compounds employing biologically specific receptors |
| US3853987A (en) | 1971-09-01 | 1974-12-10 | W Dreyer | Immunological reagent and radioimmuno assay |
| US3867517A (en) | 1971-12-21 | 1975-02-18 | Abbott Lab | Direct radioimmunoassay for antigens and their antibodies |
| NL171930C (nl) | 1972-05-11 | 1983-06-01 | Akzo Nv | Werkwijze voor het aantonen en bepalen van haptenen, alsmede testverpakkingen. |
| US3850578A (en) | 1973-03-12 | 1974-11-26 | H Mcconnell | Process for assaying for biologically active molecules |
| US3935074A (en) | 1973-12-17 | 1976-01-27 | Syva Company | Antibody steric hindrance immunoassay with two antibodies |
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4034074A (en) | 1974-09-19 | 1977-07-05 | The Board Of Trustees Of Leland Stanford Junior University | Universal reagent 2-site immunoradiometric assay using labelled anti (IgG) |
| US3984533A (en) | 1975-11-13 | 1976-10-05 | General Electric Company | Electrophoretic method of detecting antigen-antibody reaction |
| US4098876A (en) | 1976-10-26 | 1978-07-04 | Corning Glass Works | Reverse sandwich immunoassay |
| US4879219A (en) | 1980-09-19 | 1989-11-07 | General Hospital Corporation | Immunoassay utilizing monoclonal high affinity IgM antibodies |
| CA1192510A (en) | 1981-05-27 | 1985-08-27 | Lawrence E. Pelcher | Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom |
| JPS6054684A (ja) | 1983-09-05 | 1985-03-29 | Teijin Ltd | 新規dνa及びハイブリツドdνa |
| US5011771A (en) | 1984-04-12 | 1991-04-30 | The General Hospital Corporation | Multiepitopic immunometric assay |
| US4666828A (en) | 1984-08-15 | 1987-05-19 | The General Hospital Corporation | Test for Huntington's disease |
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| CA1288073C (en) | 1985-03-07 | 1991-08-27 | Paul G. Ahlquist | Rna transformation vector |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4801531A (en) | 1985-04-17 | 1989-01-31 | Biotechnology Research Partners, Ltd. | Apo AI/CIII genomic polymorphisms predictive of atherosclerosis |
| ATE141646T1 (de) | 1986-04-09 | 1996-09-15 | Genzyme Corp | Genetisch transformierte tiere, die ein gewünschtes protein in milch absondern |
| GB8608850D0 (en) | 1986-04-11 | 1986-05-14 | Diatech Ltd | Packaging system |
| JPS6314693A (ja) | 1986-07-04 | 1988-01-21 | Sumitomo Chem Co Ltd | 植物ウイルスrnaベクタ− |
| US5015580A (en) | 1987-07-29 | 1991-05-14 | Agracetus | Particle-mediated transformation of soybean plants and lines |
| DE3784860D1 (de) | 1986-12-05 | 1993-04-22 | Ciba Geigy Ag | Verbessertes verfahren zur transformation von pflanzlichen protoplasten. |
| ATE108828T1 (de) | 1987-02-09 | 1994-08-15 | Lubrizol Genetics Inc | Hybrides rns-virus. |
| US4873316A (en) | 1987-06-23 | 1989-10-10 | Biogen, Inc. | Isolation of exogenous recombinant proteins from the milk of transgenic mammals |
| US5316931A (en) | 1988-02-26 | 1994-05-31 | Biosource Genetics Corp. | Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes |
| US5416011A (en) | 1988-07-22 | 1995-05-16 | Monsanto Company | Method for soybean transformation and regeneration |
| US5693507A (en) | 1988-09-26 | 1997-12-02 | Auburn University | Genetic engineering of plant chloroplasts |
| US5272057A (en) | 1988-10-14 | 1993-12-21 | Georgetown University | Method of detecting a predisposition to cancer by the use of restriction fragment length polymorphism of the gene for human poly (ADP-ribose) polymerase |
| US5302523A (en) | 1989-06-21 | 1994-04-12 | Zeneca Limited | Transformation of plant cells |
| US5550318A (en) | 1990-04-17 | 1996-08-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US7705215B1 (en) | 1990-04-17 | 2010-04-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US5464764A (en) | 1989-08-22 | 1995-11-07 | University Of Utah Research Foundation | Positive-negative selection methods and vectors |
| US5192659A (en) | 1989-08-25 | 1993-03-09 | Genetype Ag | Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes |
| US5484956A (en) | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
| JP3209744B2 (ja) | 1990-01-22 | 2001-09-17 | デカルブ・ジェネティクス・コーポレーション | 結実能力のある遺伝子変換コーン |
| US6403865B1 (en) | 1990-08-24 | 2002-06-11 | Syngenta Investment Corp. | Method of producing transgenic maize using direct transformation of commercially important genotypes |
| US5486359A (en) | 1990-11-16 | 1996-01-23 | Osiris Therapeutics, Inc. | Human mesenchymal stem cells |
| US5384253A (en) | 1990-12-28 | 1995-01-24 | Dekalb Genetics Corporation | Genetic transformation of maize cells by electroporation of cells pretreated with pectin degrading enzymes |
| DE69334225D1 (de) | 1992-07-07 | 2008-07-31 | Japan Tobacco Inc | Verfahren zur transformation einer monokotyledon pflanze |
| US5281521A (en) | 1992-07-20 | 1994-01-25 | The Trustees Of The University Of Pennsylvania | Modified avidin-biotin technique |
| WO1994002620A2 (en) | 1992-07-27 | 1994-02-03 | Pioneer Hi-Bred International, Inc. | An improved method of agrobacterium-mediated transformation of cultured soybean cells |
| US6329191B1 (en) | 1993-08-30 | 2001-12-11 | Hawaii Biotechnology Group, Inc. | DNA encoding recombinant coffee bean alpha-galactosidase |
| US5998193A (en) | 1994-06-24 | 1999-12-07 | Gene Shears Pty., Ltd. | Ribozymes with optimized hybridizing arms, stems, and loops, tRNA embedded ribozymes and compositions thereof |
| US5693512A (en) | 1996-03-01 | 1997-12-02 | The Ohio State Research Foundation | Method for transforming plant tissue by sonication |
| US6075184A (en) | 1996-03-26 | 2000-06-13 | University Of Hawaii | Purified proteins, recombinant DNA sequences and processes for producing caffeine free beverages |
| US5886164A (en) | 1996-04-15 | 1999-03-23 | Zeneca Limited | DNA encoding enzymes related to ethylene biosynthesis and ripening from banana |
| US5981840A (en) | 1997-01-24 | 1999-11-09 | Pioneer Hi-Bred International, Inc. | Methods for agrobacterium-mediated transformation |
| WO1998042848A1 (en) | 1997-03-24 | 1998-10-01 | University Of Hawaii | Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages |
| AU6324798A (en) | 1998-02-13 | 1998-09-08 | University Of Hawaii | Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages |
| CA2359868A1 (en) | 1999-01-14 | 2000-07-20 | Monsanto Company | Soybean transformation method |
| EP1020519A1 (en) | 1999-01-15 | 2000-07-19 | Introgene B.V. | Minor histocompatibility antigens and their use in the diagnosis and treatment of tumors |
| JP3520328B2 (ja) | 2000-10-06 | 2004-04-19 | 奈良先端科学技術大学院大学長 | コーヒー属植物のテオブロミン合成酵素ポリペプチド及び当該ポリペプチドをコードする遺伝子 |
| ATE318319T1 (de) | 2001-10-10 | 2006-03-15 | Nestle Sa | Kaffeepflanze mit verringerter alpha-d- galaktosidase-aktivität |
| JP2004049022A (ja) | 2002-07-16 | 2004-02-19 | Nara Institute Of Science & Technology | 遺伝子組換えによるカフェインレスコーヒー植物の製造方法 |
| US9567591B2 (en) | 2003-05-15 | 2017-02-14 | Mello Biotechnology, Inc. | Generation of human embryonic stem-like cells using intronic RNA |
| WO2005111211A2 (en) | 2004-05-14 | 2005-11-24 | Rosetta Genomics Ltd. | Micronas and uses thereof |
| WO2006040763A2 (en) | 2004-10-12 | 2006-04-20 | Technion Research & Development Foundation Ltd. | Isolated primate embryonic cells and methods of generating and using same |
| CA2625928A1 (en) | 2005-10-14 | 2007-04-26 | James Gerard Mccarthy | Nucleic acids and proteins associated with galactomannan synthesis in coffee |
| EP2650366B1 (en) | 2005-10-18 | 2017-03-22 | Precision Biosciences | Rationally designed meganucleases with altered sequence specificity and DNA-binding affinity |
| EP1945765A2 (en) | 2005-10-28 | 2008-07-23 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Structures of active guide rna molecules and method of selection |
| WO2008148223A1 (en) | 2007-06-07 | 2008-12-11 | Agriculture And Agri-Food Canada | Nanocarrier based plant transfection and transduction |
| BRPI0817911B8 (pt) | 2007-10-05 | 2022-06-28 | Dow Agrosciences Llc | Processos para transferência de substâncias moleculares para dentro de células vegetais e processo para expressão de um gene |
| US8212019B2 (en) * | 2008-07-30 | 2012-07-03 | University Of Massachusetts | Nucleic acid silencing sequences |
| US20100293671A1 (en) | 2009-05-13 | 2010-11-18 | National Taiwan University | Composition and Method for Prolonging the Shelf Life of Banana by Using Interfering RNA |
| EP2292176B1 (en) | 2009-09-07 | 2019-01-09 | Nobel Biocare Services AG | Implantation set |
| CA2819423C (en) * | 2010-12-02 | 2020-12-22 | Keygene N.V. | Targeted alteration of dna |
| CA2899650A1 (en) | 2012-02-29 | 2013-09-06 | Benitec Biopharma Limited | Pain treatment |
| WO2013184768A1 (en) | 2012-06-05 | 2013-12-12 | University Of Georgia Research Foundation, Inc. | Compositions and methods of gene silencing in plants |
| AR092482A1 (es) | 2012-09-07 | 2015-04-22 | Dow Agrosciences Llc | Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas |
| US20150291967A1 (en) | 2012-10-31 | 2015-10-15 | Luc Mathis | Coupling herbicide resistance with targeted insertion of transgenes in plants |
| EP2931026B1 (en) | 2012-11-21 | 2025-05-07 | Nunhems B.V. | Solanum lycopersicum plants having non-transgenic alterations in the acs2 gene |
| JP2016507514A (ja) | 2013-01-08 | 2016-03-10 | ベニテック バイオファーマ リミテッド | 加齢黄斑変性の処置 |
| US20150056629A1 (en) | 2013-04-14 | 2015-02-26 | Katriona Guthrie-Honea | Compositions, systems, and methods for detecting a DNA sequence |
| WO2014186686A2 (en) | 2013-05-17 | 2014-11-20 | Two Blades Foundation | Targeted mutagenesis and genome engineering in plants using rna-guided cas nucleases |
| ES2670531T3 (es) | 2013-05-29 | 2018-05-30 | Cellectis S.A. | Un método para producir una escisión de ADN precisa utilizando la actividad nickasa de Cas9 |
| WO2014194190A1 (en) | 2013-05-30 | 2014-12-04 | The Penn State Research Foundation | Gene targeting and genetic modification of plants via rna-guided genome editing |
| EP3725885A1 (en) | 2013-06-17 | 2020-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof |
| EP3178928B1 (en) | 2013-08-07 | 2020-06-10 | Regeneron Pharmaceuticals, Inc. | Pint knockout mouse exhibiting a premature aging-associated phenotype |
| CA3221516A1 (en) | 2013-08-22 | 2015-02-26 | E. I. Du Pont De Nemours And Company | Plant genome modification using guide rna/cas endonuclease systems and methods of use |
| RU2016128077A (ru) * | 2013-12-12 | 2018-12-06 | Те Брод Инститьют Инк. | Доставка, применение и применения в терапии систем и композиций crispr-cas для лечения обусловленных hbv и вирусных заболеваний и нарушений |
| BR102013032317B1 (pt) | 2013-12-16 | 2022-04-19 | Embrapa - Empresa Brasileira De Pesquisa Agropecuária | Método, kit e oligonucleotídeos para identificação de plantas com teor de cafeína reduzido, método de gerar plantas com teor de cafeína reduzido, planta com teor reduzido de cafeína |
| EP4063503A1 (en) | 2014-02-11 | 2022-09-28 | The Regents of the University of Colorado, a body corporate | Crispr enabled multiplexed genome engineering |
| CA2956545A1 (en) | 2014-08-04 | 2016-02-11 | Fred Hutchinson Cancer Research Center | T cell immunotherapy specific for wt-1 |
| US20160076093A1 (en) | 2014-08-04 | 2016-03-17 | University Of Washington | Multiplex homology-directed repair |
| US20170354745A1 (en) | 2014-10-09 | 2017-12-14 | The Regents Of The University Of California | Targeted disruption of a csf1-dap12 pathway member gene for the treatment of neuropathic pain |
| WO2016089433A1 (en) | 2014-12-03 | 2016-06-09 | Agilent Technologies, Inc. | Guide rna with chemical modifications |
| US20170327834A1 (en) * | 2014-12-15 | 2017-11-16 | Syngenta Participations Ag | Pesticidal microrna carriers and use thereof |
| CA2972454C (en) | 2014-12-31 | 2024-09-10 | Phykion Inc. | Compositions and methods for high efficiency in in vivo genome editing |
| US20180073035A1 (en) | 2015-01-19 | 2018-03-15 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences | A method for precise modification of plant via transient gene expression |
| EP4223786A3 (en) | 2015-03-06 | 2023-08-16 | Public University Corporation Yokohama City University | Novel anti-pad4 antibodies |
| EP3303585A4 (en) | 2015-06-03 | 2018-10-31 | Board of Regents of the University of Nebraska | Dna editing using single-stranded dna |
| IL293323B2 (en) | 2015-06-18 | 2024-01-01 | Massachusetts Inst Technology | Crispr enzyme mutations reducing off-target effects |
| CN106701752B (zh) | 2015-09-01 | 2019-07-12 | 清华大学 | 人工合成的microRNA簇及其构建方法与所用合成microRNA单元 |
| US12049625B2 (en) | 2016-01-14 | 2024-07-30 | The Brigham And Women's Hospital, Inc. | Genome editing for treating glioblastoma |
| CN105647962A (zh) | 2016-02-15 | 2016-06-08 | 浙江大学 | 运用CRISPR-Cas9系统敲除水稻MIRNA393b茎环序列的基因编辑方法 |
| CN105821075B (zh) | 2016-04-22 | 2017-09-12 | 湖南农业大学 | 一种茶树咖啡因合成酶CRISPR/Cas9基因组编辑载体的构建方法 |
| CN106367435B (zh) | 2016-09-07 | 2019-11-08 | 电子科技大学 | 一种水稻miRNA定向敲除的方法 |
| AU2018225825A1 (en) | 2017-02-22 | 2019-09-12 | Université de Montréal | Novel minor histocompatibility antigens and uses thereof |
| WO2018178987A1 (en) | 2017-03-30 | 2018-10-04 | Ramot At Tel-Aviv University Ltd. | Methods of treating alzheimer's disease |
| EP3601579B1 (en) | 2017-03-31 | 2023-10-04 | Pioneer Hi-Bred International, Inc. | Expression modulating elements and use thereof |
| GB201708662D0 (en) | 2017-05-31 | 2017-07-12 | Tropic Biosciences Uk Ltd | Compositions and methods for increasing shelf-life of banana |
| WO2018220582A1 (en) | 2017-05-31 | 2018-12-06 | Tropic Biosciences UK Limited | Methods of selecting cells comprising genome editing events |
| GB201708665D0 (en) | 2017-05-31 | 2017-07-12 | Tropic Biosciences Uk Ltd | Compositions and methods for increasing extractability of solids from coffee beans |
| CA3064601A1 (en) | 2017-06-26 | 2019-01-03 | President And Fellows Of Harvard College | Crispr/cas-adenine deaminase based compositions, systems, and methods for targeted nucleic acid editing |
| SG11202002481RA (en) | 2017-09-19 | 2020-04-29 | Tropic Biosciences Uk Ltd | Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells |
| GB201807192D0 (en) | 2018-05-01 | 2018-06-13 | Tropic Biosciences Uk Ltd | Compositions and methods for reducing caffeine content in coffee beans |
| WO2020178099A1 (en) | 2019-03-01 | 2020-09-10 | Syngenta Crop Protection Ag | Suppression of target gene expression through genome editing of native mirnas |
| GB201903519D0 (en) | 2019-03-14 | 2019-05-01 | Tropic Biosciences Uk Ltd | Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest |
| GB201903521D0 (en) | 2019-03-14 | 2019-05-01 | Tropic Biosciences Uk Ltd | No title |
| GB201903520D0 (en) | 2019-03-14 | 2019-05-01 | Tropic Biosciences Uk Ltd | Modifying the specificity of non-coding rna molecules for silencing genes in eukaryotic cells |
| GB202103256D0 (en) | 2021-03-09 | 2021-04-21 | Tropic Biosciences Uk Ltd | Method for silencing genes |
-
2018
- 2018-09-18 SG SG11202002481RA patent/SG11202002481RA/en unknown
- 2018-09-18 EP EP18786034.1A patent/EP3684930B1/en active Active
- 2018-09-18 SG SG11202002479XA patent/SG11202002479XA/en unknown
- 2018-09-18 WO PCT/IB2018/057143 patent/WO2019058253A1/en not_active Ceased
- 2018-09-18 IL IL313387A patent/IL313387A/en unknown
- 2018-09-18 CN CN201880074921.9A patent/CN111448318B/zh active Active
- 2018-09-18 DK DK18786034.1T patent/DK3684930T3/da active
- 2018-09-18 JP JP2020515764A patent/JP7422069B2/ja active Active
- 2018-09-18 BR BR112020005416-0A patent/BR112020005416A2/pt unknown
- 2018-09-18 IL IL273460A patent/IL273460B2/en unknown
- 2018-09-18 AU AU2018336128A patent/AU2018336128B2/en active Active
- 2018-09-18 ES ES18786034T patent/ES2915576T3/es active Active
- 2018-09-18 KR KR1020207011255A patent/KR20200051808A/ko active Pending
- 2018-09-18 BR BR112020005444-5A patent/BR112020005444A2/pt unknown
- 2018-09-18 CA CA3074948A patent/CA3074948A1/en active Pending
- 2018-09-18 CN CN202510041501.2A patent/CN120193018A/zh active Pending
- 2018-09-18 JP JP2020515770A patent/JP7462553B2/ja active Active
- 2018-09-18 US US16/648,779 patent/US20200270604A1/en active Pending
- 2018-09-18 KR KR1020237008634A patent/KR102871442B1/ko active Active
- 2018-09-18 EP EP18786033.3A patent/EP3684929A1/en active Pending
- 2018-09-18 AU AU2018336126A patent/AU2018336126B2/en active Active
- 2018-09-18 WO PCT/IB2018/057160 patent/WO2019058255A1/en not_active Ceased
- 2018-09-18 CA CA3074946A patent/CA3074946A1/en active Pending
- 2018-09-18 US US16/648,748 patent/US11555199B2/en active Active
- 2018-09-18 KR KR1020207011282A patent/KR102510576B1/ko active Active
- 2018-09-18 KR KR1020257033737A patent/KR20250161608A/ko active Pending
- 2018-09-18 CN CN202510041499.9A patent/CN120210261A/zh active Pending
- 2018-09-18 EP EP22170070.1A patent/EP4170029A3/en active Pending
- 2018-09-18 CN CN201880073961.1A patent/CN111373044B/zh active Active
-
2020
- 2020-03-19 IL IL273461A patent/IL273461A/en unknown
-
2023
- 2023-01-10 US US18/152,718 patent/US12331295B2/en active Active
- 2023-01-10 US US18/152,722 patent/US12275939B2/en active Active
- 2023-04-04 AU AU2023202078A patent/AU2023202078B2/en active Active
- 2023-08-25 JP JP2023137627A patent/JP2023179432A/ja active Pending
-
2025
- 2025-04-01 AU AU2025202319A patent/AU2025202319A1/en active Pending
- 2025-05-16 US US19/210,663 patent/US20260009031A1/en active Pending
-
2026
- 2026-01-28 AU AU2026200565A patent/AU2026200565A1/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050203047A1 (en) * | 2004-03-10 | 2005-09-15 | Ulrich Thomann | Delivery vectors for short interfering RNA, micro-RNA and antisense RNA |
Non-Patent Citations (1)
| Title |
|---|
| JIANPING ZHOU ET AL: FRONTIERS IN PLANT SCIENCE, 2017 page 1598, <URL:https://www.frontiersin.org/articles/10.3389/fpls.2017.01598/full> DOI: 10.3389/fpls.2017.01598 * |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12331295B2 (en) | Modifying the specificity of plant non-coding RNA molecules for silencing gene expression | |
| AU2020237667B2 (en) | Introducing silencing activity to dysfunctional RNA molecules and modifying their specificity against a gene of interest | |
| AU2020236753B2 (en) | Production of dsRNA in plant cells for pest protection via gene silencing | |
| US20240309401A1 (en) | Method for silencing genes | |
| HK40028645B (en) | Modifying the specificity of plant non-coding rna molecules for silencing gene expression |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) |