DK3279310T3 - Celledyrkningsapparat og fremgangsmåde til celledyrkning - Google Patents

Celledyrkningsapparat og fremgangsmåde til celledyrkning Download PDF

Info

Publication number
DK3279310T3
DK3279310T3 DK16772117.4T DK16772117T DK3279310T3 DK 3279310 T3 DK3279310 T3 DK 3279310T3 DK 16772117 T DK16772117 T DK 16772117T DK 3279310 T3 DK3279310 T3 DK 3279310T3
Authority
DK
Denmark
Prior art keywords
cell
culture method
cultivation apparatus
cell culture
cell cultivation
Prior art date
Application number
DK16772117.4T
Other languages
English (en)
Inventor
Shinji Sugiura
Taku Satoh
Toshiyuki Kanamori
Kazumi SHIN
Original Assignee
Aist
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aist filed Critical Aist
Application granted granted Critical
Publication of DK3279310T3 publication Critical patent/DK3279310T3/da

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/12—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by pressure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/005—Incubators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/14—Pressurized fluid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0874—Three dimensional network
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/088—Channel loops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break
DK16772117.4T 2015-04-03 2016-03-08 Celledyrkningsapparat og fremgangsmåde til celledyrkning DK3279310T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2015077196 2015-04-03
PCT/JP2016/057107 WO2016158233A1 (ja) 2015-04-03 2016-03-08 細胞培養装置および細胞培養方法

Publications (1)

Publication Number Publication Date
DK3279310T3 true DK3279310T3 (da) 2021-08-02

Family

ID=57004982

Family Applications (1)

Application Number Title Priority Date Filing Date
DK16772117.4T DK3279310T3 (da) 2015-04-03 2016-03-08 Celledyrkningsapparat og fremgangsmåde til celledyrkning

Country Status (7)

Country Link
US (1) US11141708B2 (da)
EP (1) EP3279310B1 (da)
JP (1) JP6423082B2 (da)
KR (1) KR102021102B1 (da)
CN (1) CN107532131B (da)
DK (1) DK3279310T3 (da)
WO (1) WO2016158233A1 (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6823793B2 (ja) * 2016-03-08 2021-02-03 国立研究開発法人産業技術総合研究所 細胞培養装置および細胞培養方法
WO2017154880A1 (ja) * 2016-03-08 2017-09-14 国立研究開発法人産業技術総合研究所 細胞培養装置および細胞培養方法
US11261414B2 (en) * 2016-11-14 2022-03-01 Arizona Board Of Regents On Behalf Of The University Of Arizona Apparatus for reconfiguration of components in a microphysiological system
JP7001286B2 (ja) * 2017-09-13 2022-02-21 国立研究開発法人産業技術総合研究所 細胞培養装置および細胞培養方法
US11584907B2 (en) 2017-09-13 2023-02-21 National Institute Of Advanced Industrial Science And Technology Cell culture apparatus and cell culture method
WO2020037194A1 (en) * 2018-08-17 2020-02-20 Sierra Biosystems, Inc. Row-independent oligonucleotide synthesis
CN109055204B (zh) * 2018-10-19 2024-03-26 杭州捷诺飞生物科技股份有限公司 药物筛选用器官芯片
JP7246041B2 (ja) 2018-11-07 2023-03-27 ウシオ電機株式会社 細胞培養チップ及びその製造方法
JP2020110117A (ja) * 2019-01-15 2020-07-27 株式会社島津製作所 細胞処理容器及び細胞処理装置
JP7300099B2 (ja) * 2019-02-22 2023-06-29 ウシオ電機株式会社 細胞培養チップ
US20210062130A1 (en) * 2019-08-27 2021-03-04 Battelle Memorial Institute Biological culture unit
WO2021084736A1 (ja) * 2019-11-01 2021-05-06 株式会社島津製作所 細胞培養方法および細胞培養装置
WO2021084737A1 (ja) * 2019-11-01 2021-05-06 株式会社島津製作所 細胞培養装置
NL2024202B1 (en) * 2019-11-08 2021-07-20 Mimetas B V Microfluidic cell culture system
KR102370142B1 (ko) * 2020-03-23 2022-03-04 프레스티지바이오로직스 주식회사 항체 의약품 제조를 위한 배양 및 정제 공정의 하이브리드 시스템
US20230357745A1 (en) * 2020-09-23 2023-11-09 University Of Yamanashi Medical device for culturing biological sample and embedding biological sample in embedding material, kit, and culturing and embedding method
CN114015568B (zh) * 2021-10-08 2024-02-23 北京龙迈达斯科技开发有限公司 一种类器官芯片及其制备方法
GB202205709D0 (en) * 2022-04-19 2022-06-01 Keratify Ltd Biological culture unit
LU502219B1 (en) * 2022-06-03 2023-12-04 Cytena Bioprocess Solutions Co Ltd Liquid handling device for processing liquid samples
JP2024018571A (ja) * 2022-07-29 2024-02-08 国立研究開発法人産業技術総合研究所 細胞培養装置
JP2024034473A (ja) * 2022-08-31 2024-03-13 国立研究開発法人産業技術総合研究所 細胞培養装置及びその製造方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
KR20010089295A (ko) 1998-10-13 2001-09-29 마이클 알. 맥닐리 수동 유체 동역학에 의한 유체회로 및 유체회로내에서의방법
DK1392814T3 (da) * 2001-04-25 2007-09-24 Cornell Res Foundation Inc Indretninger og fremgangsmåder til farmakokinetisk baseret cellekultursystem
US6682703B2 (en) * 2001-09-05 2004-01-27 Irm, Llc Parallel reaction devices
DE10302720A1 (de) 2003-01-23 2004-08-05 Steag Microparts Gmbh Mikrofluidischer Schalter zum Anhalten des Flüssigkeitsstroms während eines Zeitintervalls
WO2007098027A2 (en) 2006-02-17 2007-08-30 Bioprocessors Corp. Microreactor with auxiliary fluid motion control
US20080153152A1 (en) * 2006-11-22 2008-06-26 Akira Wakabayashi Microfluidic chip
JP4852399B2 (ja) 2006-11-22 2012-01-11 富士フイルム株式会社 二液合流装置
EP2040073A1 (en) * 2007-09-20 2009-03-25 Iline Microsystems, S.L. Microfluidic device and method for fluid clotting time determination
SG193829A1 (en) * 2008-06-04 2013-10-30 Tissuse Gmbh Organ-on-a-chip-device
JP5374446B2 (ja) * 2010-06-08 2013-12-25 積水化学工業株式会社 微量液滴秤取構造、マイクロ流体デバイス及び微量液滴秤取方法
WO2013086329A1 (en) 2011-12-08 2013-06-13 Research Triangle Institute Human emulated response with microfluidic enhanced systems
WO2013086486A1 (en) 2011-12-09 2013-06-13 President And Fellows Of Harvard College Integrated human organ-on-chip microphysiological systems
KR101395203B1 (ko) 2012-07-10 2014-05-16 서울대학교산학협력단 미소 유체 세포배양장치와 그 제조방법, 그리고 그 세포배양장치를 이용한 세포 배양방법
KR101404657B1 (ko) 2013-04-26 2014-06-09 주식회사 바이오록스 진공, 상압 및 공압을 제공하는 랩온어칩 구동용 매니폴드 패키지
JP6230103B2 (ja) 2013-10-08 2017-11-15 国立研究開発法人産業技術総合研究所 細胞培養装置および細胞培養方法

Also Published As

Publication number Publication date
EP3279310A4 (en) 2018-12-05
WO2016158233A1 (ja) 2016-10-06
JP6423082B2 (ja) 2018-11-14
CN107532131B (zh) 2021-11-02
US11141708B2 (en) 2021-10-12
JPWO2016158233A1 (ja) 2018-02-01
CN107532131A (zh) 2018-01-02
EP3279310A1 (en) 2018-02-07
KR102021102B1 (ko) 2019-09-11
EP3279310B1 (en) 2021-05-05
US20180085726A1 (en) 2018-03-29
KR20170121293A (ko) 2017-11-01

Similar Documents

Publication Publication Date Title
DK3279310T3 (da) Celledyrkningsapparat og fremgangsmåde til celledyrkning
LU92752B1 (en) Cell culture apparatus and culture methods using same
DK3460042T3 (da) Celledyrkningsmedium til dyrkning af et organoid, dyrkningsfremgnagsmåde og organoid
DK3149025T3 (da) Cellepenetrerende peptider og fremgangsmåder til frembringelse og anvendelse deraf
DK3215601T3 (da) Fremgangsmåder til transduktion og celleforarbejdning
DK3298148T3 (da) Fremgangsmåde og konstrukter til målrettet nukleinsyreredigering i planter
EP3292750A4 (en) Sapling cultivation apparatus and sapling cultivation method
DK3122870T3 (da) Fremgangsmåder og genetiske systemer til celleteknik
DK3339479T3 (da) Elektrolyseindretning og elektrolysefremgangsmåde
DK3009510T3 (da) Fremgangsmåde og apparat til hurtig nukleinsyre-ekstraktion
DK3386550T3 (da) Fremgangsmåder til fremstilling og anvendelse af guide nukleinsyrer
SG11201705853VA (en) Cell culture device and cell culture method
ES2966757T3 (es) Métodos de reprogramación mejorados y plataformas de cultivo celular
DK3368663T3 (da) Virusfri cellelinjer og fremgangsmåder til opnåelse deraf
SG11201609916RA (en) Culture method and cell mass
DK3177876T3 (da) Fremgangsmåde og indretning til affugtning
EP3196292A4 (en) Cell culture apparatus and method
DK3240892T5 (da) Rpe-cellepopulationer og fremgangsmåder til dannelse deraf
EP3130220A4 (en) Hydroponic culturing apparatus and hydroponic culturing method
DK3253863T3 (da) Bioreaktorsystem til celledyrkning
EP3115450A4 (en) Cell culture method and cell culture device
DK3102232T3 (da) Fremgangsmåder og sammensætninger til behandling af abnorm cellevækst
DK3347310T3 (da) Afsaltningsprocesser og fremgangsmåder til gødningsproduktion
GB201717096D0 (en) Perfusion culture apparatus and perfusion culture method
DK3332940T3 (da) Silikoneekstruderingsanlæg og fremgangsmåde til silikoneekstrudering