DK2880440T3 - Bestemmelse af køn, levedygtighed og/eller udviklingstrin for fjerkræsembryoer in ovo - Google Patents
Bestemmelse af køn, levedygtighed og/eller udviklingstrin for fjerkræsembryoer in ovo Download PDFInfo
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- DK2880440T3 DK2880440T3 DK13747889.7T DK13747889T DK2880440T3 DK 2880440 T3 DK2880440 T3 DK 2880440T3 DK 13747889 T DK13747889 T DK 13747889T DK 2880440 T3 DK2880440 T3 DK 2880440T3
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Claims (13)
1. Fremgangsmåde til ikke-destruktionsbestemmelse af køn og/eller udviklingstrin for et fjerkræsembryo i et æg, hvilken fremgangsmåde omfatter (a) detektering af mindst en første udviklingsmarkør for bestemmelsen af udviklingstrin valgt fra betain, aspartat, arginin, glutamat, glutamin og prolin og/eller mindst en første udviklingsmarkør for kønsbestemmelsen valgt fra glucose, cholin og/eller valin i et ægs amnionvæske på et tidspunkt fra starten af udrugningen af ægget indtil udklækningen; (b) måling af mængden af den mindst første detekterede udviklingsmarkørforbindelse til bestemmelse af udviklingstrinnet og/eller kønnet; og (c) sammenligning af mængden med en baseline etableret for hankøn og hunkøn, og/eller udviklingstrin for embryoet, for at bestemme, om embryoet er hankøn eller hunkøn og/eller embryoets udviklingstrin.
2. Fremgangsmåde ifølge krav 1, hvor (c) indbefatter en univariat eller multivariat analyse af de målte metabolitter og en bestemmelse af associationen af et fjerkræsembryo med en bestemt population, der omfatter et hovedkomponentanalyseprogram og/eller et partielt mindste kvadraters regressionsanalyseprogram.
3. Fremgangsmåde ifølge krav 1 eller krav 2, hvor mindst en anden markør detekteres i trin (a), og hvor den mindst første og anden markør analyseres og sammenlignes med baseline og med hinanden for at fastslå et udviklingsmarkørforhold.
4. Fremgangsmåde ifølge krav 3, hvor fjerkræsarten er Gallus gallus domesticus, og hvor en første eller yderligere udviklingsmarkør er glucose i absolut mængde for et hunkønsembryo i intervallet fra 30 pM/ml til 70 μΜ/ml og for et hankønsembryo på fra 1 μΜ/ml op til 30 pM/ml.
5. Fremgangsmåde ifølge krav 4, hvor en første eller yderligere udviklingsmarkør er cholin i en absolut mængde for et hunkønsembryo i intervallet fra 110 μΜ/ml til 130 μΜ/ml og for et hankønsembryo på fra 90 μΜ/ml op til men ikke inklusive 110 μΜ/ml.
6. Fremgangsmåde ifølge krav 4 eller 5, hvor en første eller yderligere udviklingsmarkør er valin i en absolut mængde for et hunkønsembryo i intervallet fra 110 μΜ/ml til 130 μΜ/ml og for et hankønsembryo på fra 90 μΜ/ml op til men ikke inklusive 110 μΜ/ml.
7. Fremgangsmåde ifølge krav 3 til 6, hvor mindst en første og en anden udviklingsmarkør detekteres og analyseres, og hvor de absolutte mængder og forholdet mellem den mindst første og den anden markør anvendes til kønsbestemmelse.
8. Fremgangsmåde ifølge krav 7, hvor analysen udføres med en fremgangsmåde med magnetisk billeddannelse, der indbefatter nukleære resonansfremgangsmåder; ved en spektral resonansfremgangsmåde, der indbefatter infrarød eller Raman-spektroskopi; og/eller en analytisk fremgangsmåde, såsom GC eller HPLC koblet med egnede detektorer, fluorescensspektroskopi og/eller et enzymbundet immunsorbent assay.
9. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, der endvidere omfatter mindst én af; (i) bestemmelse af, om et embryo i et æg er hankøn eller hunkøn og separation af en flerhed af hankønsæg fra en flerhed af hunkønsæg for at danne en overvejende hankøns- eller overvejende hunkønsægselektion; (ii) bestemmelse af, om et embryo i et æg er hankøn eller hunkøn og separation af en flerhed af hankønsæg fra en flerhed af hunkønsæg for at danne en overvejende hankøns- eller overvejende hunkønsægselektion, og udsættelse af hunkøns- eller hankønsægselektioneme for en udrugnings- og udklækningsproces for at danne en overvejende hunkøns- eller hankønskyllingepopulation.
10. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 9, der omfatter (iii) bestemmelse af embryoets udviklingstrin og tiden indtil sandsynlig udklækning, og separation af æg i flerheder af æg med tilsvarende eller ens udviklingstrin.
11. Fremgangsmåde ifølge krav 10, der endvidere omfatter udsættelse af ægget for en udrugnings- og udklækningsproces i overensstemmelse med den forventede udklækningsdato for at danne en kyllingepopulation af overvejende samme udviklingstrin.
12. Fremgangsmåde ifølge krav 9 eller 10, der endvidere omfatter (vi) injektion af et virus eller virus-lignende materiale i hvert æg identificeret som indeholdende et levende embryo og hankøn eller hunkøn og/eller af et bestemt udviklingstrin, og (vii) isolering af den opnåede vaccine fra de udrugede æg.
13. Fremgangsmåde ifølge krav 10, hvor fjerkræsarten er Gallus gallus domesticus, og hvor udviklingsmarkøreme er valgt fra mindst én af: trimethylglycin, aspartat og/eller asparagin, glutamat og/eller glutamin og/eller prolin.
Applications Claiming Priority (4)
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| US201261677227P | 2012-07-30 | 2012-07-30 | |
| NL2009256A NL2009256C2 (en) | 2012-07-30 | 2012-07-30 | Age determination of avian embryos in ovo. |
| NL2009255A NL2009255C2 (en) | 2012-07-30 | 2012-07-30 | Gender determination of avian embryos in ovo. |
| PCT/NL2013/050569 WO2014021715A2 (en) | 2012-07-30 | 2013-07-30 | Gender, viability and/or developmental stage determination of avian embryos in ovo |
Publications (1)
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| DK2880440T3 true DK2880440T3 (da) | 2019-02-25 |
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| DE102014010150A1 (de) | 2014-07-04 | 2016-01-07 | Technische Universität Dresden | Verfahren und Vorrichtung zur Raman-spektroskopischen in-ovo Geschlechtsbestimmung von befruchteten und bebrüteten Vogeleiern |
| FR3023617B1 (fr) | 2014-07-11 | 2018-06-15 | Tronico | Dispositif non invasif de determination de la fertilite et/ou du sexe d’un oeuf, et procede correspondant. |
| WO2016020838A1 (en) | 2014-08-04 | 2016-02-11 | Gencell Biosystems Ltd. | Genomic analysis device and methods of use |
| ES2687143T3 (es) * | 2015-07-29 | 2018-10-23 | Agri Advanced Technologies Gmbh | Procedimiento y dispositivo para la incorporación de una abertura en la cáscara calcárea en la zona del polo romo de huevos de ave incubados con embrión |
| WO2017094015A1 (en) * | 2015-12-03 | 2017-06-08 | Eggxyt Ltd | Methods for gender determination of avian embryos in unhatched eggs and means thereof |
| DE102016004051B3 (de) | 2016-04-04 | 2017-07-27 | Technische Universität Dresden | Verfahren und Vorrichtung zur optischen in-ovo Geschlechtsbestimmung von befruchteten und bebrüteten Vogeleiern |
| EP3460478A4 (en) * | 2016-05-17 | 2020-01-08 | Osaka University | METHOD AND SYSTEM FOR BLOOD SAMPLING FOR DETERMINING DIABETES |
| KR102530639B1 (ko) * | 2016-05-24 | 2023-05-09 | 인 오보 홀딩 비.브이. | 난 내 가금류 성별의 비파괴적인 판단 방법 및 시스템 |
| DE102016011348A1 (de) | 2016-09-16 | 2018-03-22 | Technische Universität Dresden | Verfahren zur Klassifizierung von Spektren von Objekten mit komplexem Informationsgehalt |
| US11766029B2 (en) * | 2017-07-06 | 2023-09-26 | Seleggt Gmbh | Method for producing chicken including determining the gender of chicken embryos |
| JP6930727B2 (ja) * | 2017-07-20 | 2021-09-01 | 株式会社ナベル | 入卵前の種卵の性別指向選抜装置 |
| FR3071701B1 (fr) * | 2017-10-03 | 2023-05-12 | Nectra | Dispositif pour injection in-ovo |
| EP3483619A1 (en) * | 2017-11-13 | 2019-05-15 | Technische Universität München | Automated noninvasive determining the sex of an embryo of and the fertility of a bird's egg |
| CN108802002B (zh) * | 2018-05-08 | 2020-11-17 | 华南农业大学 | 一种快速无损鉴别解除滞育的蚕卵拉曼光谱模型构建方法 |
| NL2021022B1 (en) * | 2018-05-30 | 2019-12-10 | Seleggt Gmbh | Method for determining the gender of a chicken embryo |
| CN109618981B (zh) * | 2019-01-25 | 2021-07-27 | 天津大学 | 一种基于吸收光谱频率特征的鸡胚存活状态的检测方法 |
| KR20210137499A (ko) * | 2019-03-05 | 2021-11-17 | 더 스테이트 오브 이스라엘, 미니스트리 오브 애그리컬처 & 루럴 디벨로프먼트, 애그리컬처럴 리서치 오거니제이션, (에이.알.오.), 볼카니 센터 | 게놈-편집 조류 |
| NL2023176B1 (en) | 2019-05-22 | 2020-12-01 | In Ovo B V | Composition comprising eggs and use of such composition |
| US11774454B2 (en) | 2019-08-25 | 2023-10-03 | The Board Of Trustees Of The University Of Illinois | Non-invasive analysis of embryo metabolites |
| AU2021320661A1 (en) | 2020-08-03 | 2023-03-30 | Emgenisys, Inc. | Embryo evaluation based on real-time video |
| DE102021127696B3 (de) | 2021-10-25 | 2023-03-09 | Technische Universität Dresden, Körperschaft des öffentlichen Rechts | Verfahren und Anordnung zur in-ovo Geschlechtsbestimmung von Vogeleiern |
| KR102632163B1 (ko) | 2022-04-13 | 2024-02-01 | 오보젠 주식회사 | 가금류 알(卵)의 암수 검사 및 선별장치 그리고 이를 이용한 선별방법 |
| CN115808414A (zh) * | 2023-01-18 | 2023-03-17 | 苏州贝康医疗器械有限公司 | 一种无创评估胚胎发育质量的拉曼光谱分析方法及其应用 |
| CN117502303B (zh) * | 2023-11-30 | 2024-06-04 | 重庆华裕耀新农业科技有限公司 | 一种种蛋无损检测方法及受精信息检测系统 |
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| SU1671680A1 (ru) * | 1989-04-04 | 1991-08-23 | Всесоюзный научно-исследовательский проектно-конструкторский институт прикладной биохимии | Способ отбора аллантоисной жидкости из куриных эмбрионов |
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| UY25347A1 (es) | 1998-01-12 | 1999-05-14 | Embrex Inc | Procedimiento de inyeccion multiple para el tratamiento de embriones aviarios in ovo, y aparato automatizado para la inyeccion in ovo. |
| US6365339B1 (en) * | 1998-09-04 | 2002-04-02 | Bechtel Bwxt Idaho, Llc | Gender determination of avian embryo |
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| US7041439B2 (en) * | 2001-04-17 | 2006-05-09 | Embrex, Inc. | Methods and apparatus for selectively processing eggs having identified characteristics |
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| WO2006124456A2 (en) | 2005-05-11 | 2006-11-23 | Embrex, Inc. | Competitive particle immunoassay methods utilizing fluorescence microscopy |
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| RU2403711C2 (ru) * | 2008-04-14 | 2010-11-20 | Государственное Образовательное Учреждение Высшего Профессионального Образования "Омский Государственный Технический Университет" | Способ определения жизнеспособности зародыша яйца птицы |
| KR101090505B1 (ko) * | 2008-12-30 | 2011-12-06 | 최은아 | 난담반을 포함하는 암 예방 및 치료용 조성물 |
| JP2010264785A (ja) * | 2009-05-12 | 2010-11-25 | Kanto Auto Works Ltd | 車両用ドア開閉装置 |
| RU2406371C1 (ru) * | 2009-07-21 | 2010-12-20 | Государственное учреждение Всероссийский научно-исследовательский институт птицеперерабатывающей промышленности (ГУ ВНИИПП) | Способ получения яичного белкового продукта |
| DK2336751T3 (da) | 2009-12-16 | 2014-11-17 | Fraunhofer Ges Forschung | Fremgangsmåde til bestemmelse af kønnet af fugleæg |
| CN102210708A (zh) * | 2010-04-01 | 2011-10-12 | 王莉娜 | 中药鸡蛋外敷疗法 |
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| KR20150036781A (ko) | 2015-04-07 |
| EP2880440B1 (en) | 2018-10-31 |
| EP2880440A2 (en) | 2015-06-10 |
| RU2015106609A (ru) | 2016-09-20 |
| CA2917414A1 (en) | 2014-02-06 |
| BR112015002156B1 (pt) | 2024-01-02 |
| KR102174868B1 (ko) | 2020-11-06 |
| US20150260704A1 (en) | 2015-09-17 |
| ES2709027T3 (es) | 2019-04-12 |
| CA2917414C (en) | 2021-09-14 |
| JP6279574B2 (ja) | 2018-02-14 |
| MX2015001297A (es) | 2015-06-05 |
| PL2880440T3 (pl) | 2019-05-31 |
| AU2013297168B2 (en) | 2019-05-30 |
| AU2013297168A1 (en) | 2015-03-05 |
| JP2015523097A (ja) | 2015-08-13 |
| UA118544C2 (uk) | 2019-02-11 |
| CN104704360A (zh) | 2015-06-10 |
| RU2681536C2 (ru) | 2019-03-07 |
| ZA201501224B (en) | 2016-01-27 |
| CN104704360B (zh) | 2018-05-15 |
| WO2014021715A3 (en) | 2014-04-10 |
| TR201901409T4 (tr) | 2019-02-21 |
| MX362096B (es) | 2019-01-07 |
| US20210215663A1 (en) | 2021-07-15 |
| BR112015002156A2 (pt) | 2017-07-04 |
| WO2014021715A2 (en) | 2014-02-06 |
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