DK2336751T3 - Fremgangsmåde til bestemmelse af kønnet af fugleæg - Google Patents
Fremgangsmåde til bestemmelse af kønnet af fugleæg Download PDFInfo
- Publication number
- DK2336751T3 DK2336751T3 DK09015586.2T DK09015586T DK2336751T3 DK 2336751 T3 DK2336751 T3 DK 2336751T3 DK 09015586 T DK09015586 T DK 09015586T DK 2336751 T3 DK2336751 T3 DK 2336751T3
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- DK
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- radiation
- electromagnetic radiation
- autofluorescence
- intrinsic fluorescence
- fractal dimension
- Prior art date
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- 238000000034 method Methods 0.000 title claims description 26
- 235000013601 eggs Nutrition 0.000 title claims description 23
- 230000005855 radiation Effects 0.000 claims description 24
- 230000005670 electromagnetic radiation Effects 0.000 claims description 20
- 230000005284 excitation Effects 0.000 claims description 15
- 239000013307 optical fiber Substances 0.000 claims description 14
- 238000001514 detection method Methods 0.000 claims description 13
- 238000005311 autocorrelation function Methods 0.000 claims description 7
- 238000005259 measurement Methods 0.000 claims description 3
- 230000006911 nucleation Effects 0.000 claims 3
- 238000010899 nucleation Methods 0.000 claims 3
- 238000001727 in vivo Methods 0.000 claims 1
- 238000010791 quenching Methods 0.000 claims 1
- 230000000171 quenching effect Effects 0.000 claims 1
- 230000003595 spectral effect Effects 0.000 claims 1
- 238000004458 analytical method Methods 0.000 description 8
- 230000008569 process Effects 0.000 description 6
- 238000001228 spectrum Methods 0.000 description 6
- 230000003993 interaction Effects 0.000 description 5
- 230000003287 optical effect Effects 0.000 description 5
- 230000020509 sex determination Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 241000287828 Gallus gallus Species 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 241000271566 Aves Species 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 235000013330 chicken meat Nutrition 0.000 description 2
- 230000000875 corresponding effect Effects 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000002189 fluorescence spectrum Methods 0.000 description 2
- 238000009499 grossing Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000007477 logistic regression Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000010183 spectrum analysis Methods 0.000 description 2
- 238000004566 IR spectroscopy Methods 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000001069 Raman spectroscopy Methods 0.000 description 1
- 238000012896 Statistical algorithm Methods 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000013145 classification model Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000009374 poultry farming Methods 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
- 230000000191 radiation effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000004335 scaling law Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K45/00—Other aviculture appliances, e.g. devices for determining whether a bird is about to lay
- A01K45/007—Injecting or otherwise treating hatching eggs
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/08—Eggs, e.g. by candling
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6484—Optical fibres
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/069—Supply of sources
- G01N2201/0696—Pulsed
- G01N2201/0697—Pulsed lasers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
- G01N2201/129—Using chemometrical methods
- G01N2201/1293—Using chemometrical methods resolving multicomponent spectra
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Environmental Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Birds (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Claims (8)
1. Fremgangsmåde til in ovo-bestemmelse af kønnet af fugleæg, kendetegnet ved, at der med en strålingskilde udsendes en elektromagnetisk stråling på kimskiven (1) af et ikke-ruget æg, og afklingeadfærden for egenfluorescensintensi-teten, der exciteres via den elektromagnetiske stråling, efter en afbrydelse af strålingskilden (5) registreres tids- og spektralopløst på det bestrålede område af kimskiven for mindst en bølgelængde af egenfluorescensen med en detektor (4), og den fraktale dimension DF beregnes med de fastlagte intensitetsmåleværdier, og værdien af den fraktale dimension DF sammenlignes med en art- og kønsspecifik grænseværdi; hvor det pågældende æg ved overskridelse af grænseværdien klassificeres som hunæg og ved underskridelse som hanæg.
2. Fremgangsmåde ifølge krav 1, kendetegnet ved, at den fraktale dimension Df1 bestemmes ved fastlæggelse af de mindste fej I kvad rater.
3. Fremgangsmåde ifølge krav 1, kendetegnet ved, at den fraktale dimension Df2 bestemmes ved fastlæggelse af differens-autokorrelations-funktionen C(t) for egenfluorescensintensitetsafklingeadfærden.
4. Fremgangsmåde ifølge et af de foregående krav, kendetegnet ved, at kimskiven (1) bestråles med en monokromatisk elektromagnetisk stråling, og der med henblik på bestemmelsen af egenfluorescensen anvendes et fluorescensspektrometer (4), hvor bestrålingen foregår på en sådan måde, at strålen til excitering af egenfluorescensen rammer kimskiven således, at der ikke reflekteres en stråling på den samme akse.
5. Fremgangsmåde ifølge et af de foregående krav, kendetegnet ved, at detekteringen gennemføres med en kendt konstant aftastningshyppighed.
6. Fremgangsmåde ifølge krav 5, kendetegnet ved, at detekteringen gennemføres med en konstant aftastningshyppighed i området 50 ps til 1000 ps.
7. Fremgangsmåde ifølge et af de foregående krav, kendetegnet ved, at der med henblik på exciteringen af fluorescensen anvendes en elektromagnetisk stråling fra UV-strålingens bølgelængdeområde.
8. Fremgangsmåde ifølge et af de foregående krav, kendetegnet ved, at den elektromagnetiske stråling, der anvendes til exciteringen af egenfluorescensen, ved hjælp af en første optisk fiber (2) rettes mod kimskiven (1), og den elektromagnetiske stråling, der forekommer som følge af egenfluorescens, via en anden optisk fiber (3) rettes mod detektoren (4), og de to optiske fibre (2 og 3) derved er orienteret i forhold til hinanden i en vinkel, der hælder skråt.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09015586.2A EP2336751B1 (de) | 2009-12-16 | 2009-12-16 | Verfahren zur Bestimmung des Geschlechts an Vogeleiern |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2336751T3 true DK2336751T3 (da) | 2014-11-17 |
Family
ID=41683040
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK09015586.2T DK2336751T3 (da) | 2009-12-16 | 2009-12-16 | Fremgangsmåde til bestemmelse af kønnet af fugleæg |
Country Status (3)
| Country | Link |
|---|---|
| US (1) | US8364247B2 (da) |
| EP (1) | EP2336751B1 (da) |
| DK (1) | DK2336751T3 (da) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102803952A (zh) | 2009-06-25 | 2012-11-28 | 耶路撒冷希伯来大学伊萨姆研发有限公司 | 卵能育性和性别的高光谱识别 |
| DE102010006161B3 (de) * | 2010-01-21 | 2011-01-13 | Technische Universität Dresden | Verfahren und Vorrichtung zur Bestimmung des Geschlechtes von befruchteten und nicht bebrüteten Vogeleiern |
| MX362096B (es) * | 2012-07-30 | 2019-01-07 | In Ovo B V | Determinacion de género, viabilidad y/o etapa de desarrollo de embriones aviares in ovo. |
| EP3757552A1 (en) * | 2012-08-31 | 2020-12-30 | Agri Advanced Technologies GmbH | Spectrophotometric analysis of embryonic chick feather color |
| DE102014010150A1 (de) | 2014-07-04 | 2016-01-07 | Technische Universität Dresden | Verfahren und Vorrichtung zur Raman-spektroskopischen in-ovo Geschlechtsbestimmung von befruchteten und bebrüteten Vogeleiern |
| FR3023617B1 (fr) | 2014-07-11 | 2018-06-15 | Tronico | Dispositif non invasif de determination de la fertilite et/ou du sexe d’un oeuf, et procede correspondant. |
| DE102016004051B3 (de) | 2016-04-04 | 2017-07-27 | Technische Universität Dresden | Verfahren und Vorrichtung zur optischen in-ovo Geschlechtsbestimmung von befruchteten und bebrüteten Vogeleiern |
| WO2017204636A2 (en) | 2016-05-24 | 2017-11-30 | In Ovo B.V. | Method and system for the non-destructive in ovo determination of fowl gender |
| CN106198448A (zh) * | 2016-07-17 | 2016-12-07 | 北京化工大学 | 一种自动高速无损分拣活体雌雄蚕蛹或者活体雌雄蚕茧的工艺 |
| DE102016011348A1 (de) | 2016-09-16 | 2018-03-22 | Technische Universität Dresden | Verfahren zur Klassifizierung von Spektren von Objekten mit komplexem Informationsgehalt |
| DE102018001987A1 (de) | 2018-03-11 | 2019-09-12 | Evonta-Technology Gmbh | Einrichtung zur Untersuchung von Bruteiern ab dem sechzehnten Bebrütungstag |
| DE202018001316U1 (de) | 2018-03-11 | 2018-04-03 | Evonta-Technology Gmbh | Einrichtung zur Untersuchung von Bruteiern ab dem sechzehnten Bebrütungstag |
| HUE062663T2 (hu) | 2018-03-11 | 2023-12-28 | Evonta Tech Gmbh | Keltetõtojások vizsgálatára szolgáló berendezés |
| WO2020095394A1 (ja) * | 2018-11-07 | 2020-05-14 | 八木 宜英 | 有精卵雌雄鑑定装置、有精卵雌雄鑑定方法、及びプログラム |
| FR3095519B1 (fr) | 2019-04-25 | 2021-03-26 | Commissariat Energie Atomique | Procédé non-invasif de détermination du genre d'un œuf |
| EP3772645B1 (de) * | 2019-08-09 | 2023-02-08 | mfd Diagnostics GmbH | Verfahren und vorrichtung zur erfassung von sehr geringer fluoreszenz |
| DE102019009002A1 (de) * | 2019-12-20 | 2021-06-24 | Evonta-Technology Gmbh | Einrichtung zur Untersuchung von Vogeleiern |
| DE102020000214A1 (de) * | 2020-01-15 | 2021-07-15 | Technische Hochschule Ostwestfalen-Lippe | Vorrichtung und Verfahren zur in-ovo Geschlechtsbestimmung bei einem befruchteten Vogelei |
| FR3135527A1 (fr) | 2022-05-12 | 2023-11-17 | Commissariat A L'energie Atomique Et Aux Energies Alternatives | Procédé et système de détermination d'une caractéristique d'un œuf d'un être vivant |
| DE202024000107U1 (de) | 2024-01-03 | 2024-02-26 | Evonta-Technology Gmbh | Einrichtung zur Untersuchung von Bruteiern |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5648468A (en) * | 1990-03-09 | 1997-07-15 | Spaulding; Glenn F. | Process for identifying sex associated egg proteins and methods for increasing the probability that the avian offspring will be the desired sex |
| US5679514A (en) * | 1992-12-31 | 1997-10-21 | Texas Tech University | Method for determining the sex of birds |
| EP1021717B1 (en) | 1996-10-04 | 2002-04-10 | Embrex Inc. | Method of sorting birds in ovo |
| US6029080A (en) * | 1997-07-09 | 2000-02-22 | Reynnells; Richard D. | Method and apparatus for avian pre-hatch sex determination |
| US6396938B1 (en) * | 1998-02-27 | 2002-05-28 | University Of Arkansas, N.A. | Automatic feather sexing of poultry chicks using ultraviolet imaging |
| US6365339B1 (en) * | 1998-09-04 | 2002-04-02 | Bechtel Bwxt Idaho, Llc | Gender determination of avian embryo |
| AU3841301A (en) * | 2000-02-15 | 2001-08-27 | Ovo Biosciences Inc | In ovo activation of an avian egg in the shell |
| US6535277B2 (en) * | 2000-12-20 | 2003-03-18 | Embrex, Inc. | Methods and apparatus for non-invasively identifying conditions of eggs via multi-wavelength spectral comparison |
| US20030200932A1 (en) * | 2001-02-16 | 2003-10-30 | Toelken L. Taizo | Ultrasound quality inspection of avian eggs |
| US6512839B1 (en) * | 2001-02-21 | 2003-01-28 | Ultra-Hatch, Inc. | Ultrasound sex determination of avian hatchlings |
| US7041439B2 (en) * | 2001-04-17 | 2006-05-09 | Embrex, Inc. | Methods and apparatus for selectively processing eggs having identified characteristics |
| JP4128791B2 (ja) * | 2002-03-27 | 2008-07-30 | 株式会社堀内 | 有精卵の雌雄鑑別技術 |
| US8238993B2 (en) | 2002-04-04 | 2012-08-07 | Veralight, Inc. | Determination of a measure of a glycation end-product or disease state using tissue fluorescence lifetime |
| GB0218955D0 (en) * | 2002-08-14 | 2002-09-25 | Roslin Inst Edinburgh | Avian sex determination method |
| EP3037807B1 (en) | 2006-10-27 | 2021-03-31 | Ramot at Tel Aviv University Ltd. | Method and system for detecting a target within a population of molecules |
| US20120135873A1 (en) * | 2007-01-29 | 2012-05-31 | Noga Porat | Genome determination assay |
| DE102007013107B4 (de) | 2007-03-15 | 2011-02-10 | Friedrich-Schiller-Universität Jena | Verfahren zur Bestimmung des Geschlechts von Vögeln |
| US7950349B1 (en) * | 2008-01-18 | 2011-05-31 | Jack Dean Rollins | Avian egg fertility and gender detection |
| BRPI1008197A2 (pt) * | 2009-02-08 | 2016-05-03 | Murdoch Childrens Res Inst | determinação de sexo e métodos de especificação do mesmo. |
| EP2405740B1 (en) * | 2009-03-13 | 2015-12-23 | Van de Ven Beheer B.V. | Method for avian sex determination |
-
2009
- 2009-12-16 DK DK09015586.2T patent/DK2336751T3/da active
- 2009-12-16 EP EP09015586.2A patent/EP2336751B1/de active Active
-
2010
- 2010-12-06 US US12/960,678 patent/US8364247B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US8364247B2 (en) | 2013-01-29 |
| EP2336751B1 (de) | 2014-09-10 |
| EP2336751A1 (de) | 2011-06-22 |
| US20110144473A1 (en) | 2011-06-16 |
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