DK2840142T3 - Gener og anvendelser til planteforbedring - Google Patents

Gener og anvendelser til planteforbedring Download PDF

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Publication number
DK2840142T3
DK2840142T3 DK14181041.6T DK14181041T DK2840142T3 DK 2840142 T3 DK2840142 T3 DK 2840142T3 DK 14181041 T DK14181041 T DK 14181041T DK 2840142 T3 DK2840142 T3 DK 2840142T3
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plants
plant
recombinant dna
transgenic
protein
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DK14181041.6T
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English (en)
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Marie Coffin
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Monsanto Technology Llc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (8)

1. Plantecellekerne med stabilt integreret, rekombinant DNA, hvor det rekom-binante DNA omfatter en promoter, der er funktionel i plantecellen, og som er operabelt forbundet med et protein-kodende DNA, der koder for en aminosy-resekvens med mindst 90 % sekvensidentitet i den fulde længde af SEQ ID NO: 1018, hvor transgene planter med det rekombinante DNA i kernerne har en øget varmetolerance sammenlignet med kontrolplanter, der ikke har det rekombinante DNA i kernerne.
2. Rekombinant DNA-konstrukt, som omfatter en promotor, der er funktionel i plantecellen, og som er operabelt forbundet med et protein-kodende DNA, der koder for en aminosyresekvens med mindst 90 % sekvensidentitet i den fulde længde af SEQ ID NO: 1018, hvor transgene planter med det rekombinante DNA i kernerne har en øget varmetolerance sammenlignet med kontrolplanter, der ikke har det rekombinante DNA i kernerne.
3. Plantecellekerne ifølge krav 1, som omfatter det rekombinante DNA-kon-strukt, der omfatter en promoter, der er funktionel i plantecellen, og som er operabelt forbundet med et protein-kodende DNA, der koder for en aminosyresekvens med mindst 90 % sekvensidentitet i den fulde længde af SEQ ID NO: 1018, yderligere omfattende DNA, der eksprimerer et protein, der tilvejebringer tolerance over for ukrudtsmiddel til plantecellen.
4. Plantecellekerne ifølge krav 3, hvor midlet i ukrudtsmidlet er en glyphosat-, dicamba- eller glufosinatforbindelse.
5. Transgent frø, omfattende en flerhed af planteceller med en plantecellekerne, omfattende et rekombinant DNA-konstrukt, der omfatter en promoter, der er funktionel i plantecellen, og som er operabelt forbundet med et proteinkodende DNA, der koder for en aminosyresekvens med mindst 90 % sekvensidentitet i den fulde længde af SEQ ID NO: 1018, hvor transgene planter med det rekombinante DNA i kernerne har en øget varmetolerance sammenlignet med kontrolplanter, der ikke har det rekombinante DNA i kernerne.
6. Transgent frø ifølge krav 5, der kan anvendes til at fremstille en afgrøde af transgene planter som resultat af ekspression af rekombinant DNA i en kerne, omfattende et rekombinant DNA-konstrukt, der omfatter en promoter, som er funktionel i plantecellen, og som er operabelt forbundet med et protein-kodende DNA, der koder for en aminosyresekvens med mindst 90 % sekvensidentitet i den fulde længde af SEQ ID NO: 1018, hvor det transgene frø kan opnås ved en fremgangsmåde omfattende: (a) at screene en population af planter for det rekombinante DNA, (b) at udvælge fra populationen en eller flere planter, der indeholder det rekombinante DNA, og (c) at indsamle frø fra planter, der er udvalgt i trin (b).
7. Transgent frø ifølge krav 6, hvor fremgangsmåden til at fremstille det transgene frø yderligere omfatter (d) verificering af, at det rekombinante DNA er stabilt integreret i den udvalgte plante, og (e) analyse af væv fra den udvalgte plante for at bestemme ekspressionen eller suppressionen af et protein med funktion af et protein med en aminosyresekvens af SEQ ID NO: 1018.
8. Transgent frø ifølge krav 5 eller 6, hvor frøet er udvalgt fra gruppen bestående af majs-, sojabønne-, bomulds-, alfalfa-, raps-, hvede- og risfrø.
DK14181041.6T 2007-06-06 2008-06-05 Gener og anvendelser til planteforbedring DK2840142T3 (da)

Applications Claiming Priority (2)

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US93342807P 2007-06-06 2007-06-06
EP13174081.3A EP2698433A1 (en) 2007-06-06 2008-06-05 Genes and uses for plant enhancement

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DK2840142T3 true DK2840142T3 (da) 2019-03-18

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US (1) US20110214199A1 (da)
EP (5) EP2543735A1 (da)
DK (1) DK2840142T3 (da)
ES (1) ES2716864T3 (da)
WO (1) WO2008153927A2 (da)

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