DK2729482T3 - PROCEDURE FOR CLEANING FC-FUSION PROTEIN - Google Patents
PROCEDURE FOR CLEANING FC-FUSION PROTEIN Download PDFInfo
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- DK2729482T3 DK2729482T3 DK12811874.2T DK12811874T DK2729482T3 DK 2729482 T3 DK2729482 T3 DK 2729482T3 DK 12811874 T DK12811874 T DK 12811874T DK 2729482 T3 DK2729482 T3 DK 2729482T3
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- Prior art keywords
- protein
- chromatography
- fusion protein
- product
- resin
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Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70578—NGF-receptor/TNF-receptor superfamily, e.g. CD27, CD30, CD40, CD95
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Peptides Or Proteins (AREA)
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| US201161505710P | 2011-07-08 | 2011-07-08 | |
| PCT/US2012/045339 WO2013009526A1 (en) | 2011-07-08 | 2012-07-03 | Method for purifying fc-fusion protein |
Publications (1)
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|---|---|
| DK2729482T3 true DK2729482T3 (en) | 2018-05-07 |
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| DK12811874.2T DK2729482T3 (en) | 2011-07-08 | 2012-07-03 | PROCEDURE FOR CLEANING FC-FUSION PROTEIN |
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| EP (1) | EP2729482B1 (OSRAM) |
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| CA (1) | CA2840951A1 (OSRAM) |
| DK (1) | DK2729482T3 (OSRAM) |
| WO (1) | WO2013009526A1 (OSRAM) |
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| SG11201501460RA (en) * | 2012-09-11 | 2015-04-29 | Coherus Biosciences Inc | Correctly folded etanercept in high purity and excellent yield |
| SI2969099T2 (sl) | 2013-03-14 | 2022-04-29 | Amgen Inc. | Odstranjevanje izluženega afinitetnega purifikacijskega liganda |
| MX362922B (es) * | 2013-03-26 | 2019-02-25 | Coherus Biosciences Inc | Método de producción de proteínas. |
| EP3102589A1 (en) * | 2014-02-04 | 2016-12-14 | Biogen MA Inc. | Use of cation-exchange chromatography in the flow-through mode to enrich post-translational modifications |
| US11389783B2 (en) | 2014-05-02 | 2022-07-19 | W.R. Grace & Co.-Conn. | Functionalized support material and methods of making and using functionalized support material |
| CN112588116A (zh) | 2014-05-13 | 2021-04-02 | 美国安进公司 | 用于过滤器和过滤过程的过程控制系统和方法 |
| CN106536565A (zh) * | 2014-06-13 | 2017-03-22 | 鲁宾有限公司 | 一种用于纯化TNFR‑Fc融合蛋白的方法 |
| FR3025515B1 (fr) * | 2014-09-05 | 2016-09-09 | Lab Francais Du Fractionnement | Procede de purification d'un anticorps monoclonal |
| US11053504B2 (en) * | 2014-10-24 | 2021-07-06 | The University Of Chicago | Heat-inducible self-assembling protein domains |
| US20160130324A1 (en) | 2014-10-31 | 2016-05-12 | Shire Human Genetic Therapies, Inc. | C1 Inhibitor Fusion Proteins and Uses Thereof |
| US11566082B2 (en) | 2014-11-17 | 2023-01-31 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| AU2015372801B2 (en) * | 2014-12-31 | 2018-12-20 | Lg Chem, Ltd. | Method for preparing tnfr-fc fusion protein containing target content of impurities |
| CN107921407B (zh) * | 2015-06-05 | 2022-07-05 | 格雷斯公司 | 生物处理吸附澄清剂及其制备和使用方法 |
| KR102645625B1 (ko) | 2015-09-22 | 2024-03-07 | 제넨테크, 인크. | Fc-함유 단백질의 발현 |
| US20170101435A1 (en) * | 2015-10-13 | 2017-04-13 | Therapeutic Proteins International, LLC | Harvesting and perfusion apparatus |
| MA43270A (fr) | 2015-11-19 | 2021-06-02 | Takeda Pharmaceuticals Co | Inhibiteur de la c1 estérase humaine recombinante et ses utilisations |
| US10730908B2 (en) | 2016-05-11 | 2020-08-04 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| JP7106187B2 (ja) | 2016-05-11 | 2022-07-26 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックスを保存する方法 |
| JP7031934B2 (ja) | 2016-05-11 | 2022-03-08 | サイティバ・バイオプロセス・アールアンドディ・アクチボラグ | 分離マトリックス |
| US10654887B2 (en) | 2016-05-11 | 2020-05-19 | Ge Healthcare Bio-Process R&D Ab | Separation matrix |
| US10889615B2 (en) | 2016-05-11 | 2021-01-12 | Cytiva Bioprocess R&D Ab | Mutated immunoglobulin-binding polypeptides |
| US11753438B2 (en) | 2016-05-11 | 2023-09-12 | Cytiva Bioprocess R&D Ab | Method of cleaning and/or sanitizing a separation matrix |
| US10513537B2 (en) | 2016-05-11 | 2019-12-24 | Ge Healthcare Bioprocess R&D Ab | Separation matrix |
| US10703774B2 (en) | 2016-09-30 | 2020-07-07 | Ge Healthcare Bioprocess R&D Ab | Separation method |
| IL285146B (en) * | 2016-07-22 | 2022-09-01 | Amgen Inc | Methods for purifying proteins involving fc |
| US12448411B2 (en) | 2016-09-30 | 2025-10-21 | Cytiva Bioprocess R&D Ab | Separation method |
| CN109929027B (zh) * | 2017-12-15 | 2020-10-09 | 山东博安生物技术有限公司 | 采用线性洗脱步骤的重组融合蛋白纯化方法 |
| CN109929038B (zh) * | 2017-12-15 | 2020-10-09 | 山东博安生物技术有限公司 | Vegf捕获剂融合蛋白的纯化方法 |
| US12233356B2 (en) * | 2019-03-21 | 2025-02-25 | Lonza Sales Ag | Process for preparing extracellular vesicles |
| KR20220103967A (ko) * | 2019-11-22 | 2022-07-25 | 모르포시스 아게 | 항체 크로마토그래피 동안의 용리액 수집 |
| CA3182368A1 (en) | 2020-05-01 | 2021-11-04 | Kashiv Biosciences, Llc | An improved process of purification of protein |
| US11416468B2 (en) * | 2020-07-21 | 2022-08-16 | International Business Machines Corporation | Active-active system index management |
| CN112574321B (zh) * | 2020-12-30 | 2023-10-20 | 上海赛金生物医药有限公司 | 一种捕获单克隆抗体-肿瘤坏死因子融合蛋白的亲和纯化方法 |
| MA71665A (fr) * | 2021-02-11 | 2025-05-30 | Denali Therapeutics Inc. | Protéines de fusion du récepteur anti-transferrine et leurs procédés d'utilisation |
| AU2022358612A1 (en) * | 2021-09-28 | 2024-04-11 | Kashiv Biosciences, Llc | An improved process of purification of fusion protein |
| CA3233422A1 (en) * | 2021-09-28 | 2023-04-06 | Kashiv Biosciences, Llc | An improved process for purification of fusion protein |
| CN114891061A (zh) * | 2022-04-25 | 2022-08-12 | 杭州艾策生物技术有限公司 | 一种糖基化重组蛋白的纯化方法 |
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| WO1998031806A2 (en) * | 1997-01-21 | 1998-07-23 | Human Genome Sciences, Inc. | Fc RECEPTORS AND POLYPEPTIDES |
| EP1006183A1 (en) * | 1998-12-03 | 2000-06-07 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Recombinant soluble Fc receptors |
| JP4363986B2 (ja) * | 2001-12-21 | 2009-11-11 | イミュネックス・コーポレーション | タンパク質の精製方法 |
| CA2492267C (en) * | 2002-07-15 | 2013-09-10 | Immunex Corporation | Methods and media for controlling sialylation of proteins produced by mammalian cells |
| GB0304576D0 (en) * | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
| CA2542951C (en) * | 2003-10-24 | 2016-08-30 | Abhinav A. Shukla | Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction |
| EP1756155A2 (en) * | 2004-04-14 | 2007-02-28 | F.Hoffmann-La Roche Ag | PURIFIED INTERLEUKIN-15/Fc FUSION PROTEIN AND PREPARATION THEREOF |
| CN101541825B (zh) | 2006-08-28 | 2013-08-14 | 阿雷斯贸易股份有限公司 | Fc融合蛋白的纯化方法 |
| JP2010501622A (ja) * | 2006-08-28 | 2010-01-21 | アレス トレーディング ソシエテ アノニム | Ｆｃ−融合タンパク質の精製法 |
| US20080167450A1 (en) * | 2007-01-05 | 2008-07-10 | Hai Pan | Methods of purifying proteins |
| JP5155453B2 (ja) * | 2008-09-15 | 2013-03-06 | イー・エム・デイー・ミリポア・コーポレイシヨン | タンパク質をベースとするアフィニティークロマトグラフィー樹脂からのタンパク質漏出を定量するための方法 |
| WO2010127069A1 (en) * | 2009-04-29 | 2010-11-04 | Schering Corporation | Antibody purification |
| SG177577A1 (en) | 2009-08-07 | 2012-03-29 | Emd Millipore Corp | Methods for purifying a target protein from one or more impurities in a sample |
| US9540426B2 (en) | 2009-10-06 | 2017-01-10 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
-
2012
- 2012-07-03 CA CA2840951A patent/CA2840951A1/en not_active Abandoned
- 2012-07-03 WO PCT/US2012/045339 patent/WO2013009526A1/en not_active Ceased
- 2012-07-03 AU AU2012282960A patent/AU2012282960A1/en not_active Abandoned
- 2012-07-03 EP EP12811874.2A patent/EP2729482B1/en not_active Not-in-force
- 2012-07-03 US US14/131,280 patent/US9556258B2/en not_active Expired - Fee Related
- 2012-07-03 DK DK12811874.2T patent/DK2729482T3/en active
- 2012-07-03 JP JP2014519246A patent/JP6280499B2/ja not_active Expired - Fee Related
- 2012-07-03 KR KR1020147002981A patent/KR20140043934A/ko not_active Withdrawn
- 2012-07-03 BR BR112014000352A patent/BR112014000352A2/pt not_active IP Right Cessation
- 2012-07-03 CN CN201280043885.2A patent/CN103814044A/zh active Pending
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2017
- 2017-07-14 AU AU2017204893A patent/AU2017204893A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2013009526A1 (en) | 2013-01-17 |
| EP2729482A1 (en) | 2014-05-14 |
| EP2729482B1 (en) | 2018-03-07 |
| CN103814044A (zh) | 2014-05-21 |
| US20140187751A1 (en) | 2014-07-03 |
| KR20140043934A (ko) | 2014-04-11 |
| JP2014520814A (ja) | 2014-08-25 |
| US9556258B2 (en) | 2017-01-31 |
| AU2017204893A1 (en) | 2017-08-03 |
| JP6280499B2 (ja) | 2018-02-14 |
| AU2012282960A1 (en) | 2014-01-16 |
| CA2840951A1 (en) | 2013-01-17 |
| EP2729482A4 (en) | 2015-02-25 |
| BR112014000352A2 (pt) | 2017-02-14 |
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