DK2630249T3 - Termostabil trichoderma-cellulase - Google Patents
Termostabil trichoderma-cellulase Download PDFInfo
- Publication number
- DK2630249T3 DK2630249T3 DK11835033.9T DK11835033T DK2630249T3 DK 2630249 T3 DK2630249 T3 DK 2630249T3 DK 11835033 T DK11835033 T DK 11835033T DK 2630249 T3 DK2630249 T3 DK 2630249T3
- Authority
- DK
- Denmark
- Prior art keywords
- polypeptide
- interest
- thermostable
- trichoderma
- cellulase
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (9)
- TERMOSTABIL TRICHODERMA-CELLULASE E Fremgangsmåde til fremstilling af et termostabilt polypeptid af interesse i en modificeret Trichoderma-værtscelle, hvilken fremgangsmåde omfatter: en modifikation, der i alt væsentligt reducerer eller forhindrer produktion af en termostabil endoglucananse V (EGV) ved hjælp af værtscellen, en exogent nukleinsyremolekyle, der koder det termostabile polypeptid af interesse operativt bundet til en promoter, hvorved mindst ét endogent polypeptid og polypeptidet af interesse hvert udtrykkes in vivo af Trichoderma-væitscellen, hvilken fremgangsmåde omfatter: inkubation af den modificerede værtscelle i et medium, der er egnet for den modificerede værtscelle til at producere det termostabile polypeptid af interesse og det endogene polypeptid, hvorved hvert af det endogene polypeptid og det termostabile polypeptid af interesse udtrykkes af den modificerede værtscelle; og udsættelse af det termostabile polypeptid af interesse og det endogene polypeptid for en forhøjet temperatur, hvor den forhøjede temperatur er tilstrækkelig til i alt væsentligt at inaktivere det endogene polypeptid, men utilstrækkelig til at inaktivere det termostabile polypeptid af interesse og EGV, hvis den er til stede; hvorved det termostabile polypeptid af interesse produceres i en aktiv eller funktionel form i alt væsentligt i fravær af aktivitet fra det endogene polypeptid.
- 2. Fremgangsmåde ifølge krav 1, der endvidere omfatter isolering af en proteinblanding fra den modificerede Trichoderma-væitscelle før trinnet med udsættelse af polypeptidet af interesse og det endogene polypeptid for den forhøjede temperatur.
- 3. Fremgangsmåde ifølge krav 1 eller krav 2, hvor den forhøjede temperatur er en temperatur på ca. 90 °C eller højere.
- 4. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 3, hvor modifikationen omfatter én/ét eller flere deletioner eller brud i nukleotider, der er involveret i ekspression af EGV.
- 5. Fremgangsmåde ifølge krav 3, hvor bruddet eller deletionen omfatter deletion eller brud af det gen, der koder, eller kodningsområdet for eller promoteren eller regulatoriske elementer til ekspression af EGV.
- 6. Fremgangsmåde ifølge krav 5, hvor deletionen eller bruddet omfatter deletion eller brud af egl5.
- 7. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor Trichoderma er T. reesei.
- 8. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor polypeptidet af interesse er hydrophobin II.
- 9. Fremgangsmåde ifølge krav 8, hvor hydrophobin II stammer fra en Trichoderma spp., eventuelt hvor Trichoderma spp. er Trichoderma reesei.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US39494610P | 2010-10-20 | 2010-10-20 | |
| EP10188285 | 2010-10-20 | ||
| PCT/US2011/056810 WO2012054554A2 (en) | 2010-10-20 | 2011-10-19 | Thermostable trichoderma cellulase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2630249T3 true DK2630249T3 (da) | 2018-12-17 |
Family
ID=44022886
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK11835033.9T DK2630249T3 (da) | 2010-10-20 | 2011-10-19 | Termostabil trichoderma-cellulase |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US20120107872A1 (da) |
| EP (1) | EP2630249B1 (da) |
| JP (1) | JP2014503185A (da) |
| KR (1) | KR20130132416A (da) |
| CN (1) | CN103562402A (da) |
| AU (1) | AU2011317171B2 (da) |
| BR (1) | BR112013009532A2 (da) |
| CA (1) | CA2815070A1 (da) |
| DK (1) | DK2630249T3 (da) |
| MX (1) | MX2013004203A (da) |
| RU (1) | RU2013122800A (da) |
| WO (1) | WO2012054554A2 (da) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20150018517A (ko) * | 2012-05-21 | 2015-02-23 | 다니스코 유에스 인크. | 트리코데르마 하이드로포빈의 생성 |
| WO2015020876A1 (en) | 2013-08-08 | 2015-02-12 | Danisco Us Inc. | Increasing the expression of a transgene in eukaryotic cells by reducing rna interference |
| WO2015051121A1 (en) | 2013-10-02 | 2015-04-09 | E. I. Du Pont De Nemours And Company | Hydrophobin composition and process for treating surfaces |
| CN106011114B (zh) * | 2016-07-28 | 2019-05-24 | 江南大学 | 一种提高普鲁兰酶稳定性的发酵液处理方法 |
| EP3523415A1 (en) * | 2016-10-04 | 2019-08-14 | Danisco US Inc. | Protein production in filamentous fungal cells in the absence of inducing substrates |
| KR102155046B1 (ko) * | 2018-06-18 | 2020-09-11 | 국민대학교산학협력단 | 고활성 셀룰레이스를 생산하는 트리코더마 속 kmf006 균주 |
| CN111944790B (zh) * | 2020-07-01 | 2022-09-09 | 深圳润康生态环境股份有限公司 | 中性蛋白酶基因、中性蛋白酶及其制备方法和应用 |
| CN112899206B (zh) * | 2021-04-12 | 2022-07-26 | 西北农林科技大学 | 一株产几丁质酶、产吲哚乙酸的芽孢杆菌及其应用和方法 |
| WO2023039358A1 (en) | 2021-09-09 | 2023-03-16 | Dupont Nutrition Biosciences Aps | Over expression of foldases and chaperones improves protein production |
| CN115838632B (zh) * | 2022-07-14 | 2024-07-12 | 湖北民族大学 | 一株美网柄牛肝菌内生木霉真菌及其应用 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5650322A (en) | 1990-10-05 | 1997-07-22 | Genencor International, Inc. | Methods for stonewashing fabrics using endoglucanases |
| ES2105925B1 (es) | 1993-05-12 | 1998-06-01 | Blas Burguet Rodrigo | Dispositivo cortador de pan transportado en carros. |
| US6017870A (en) * | 1996-10-09 | 2000-01-25 | Genencor International, Inc. | Purified cellulase and method of producing |
| GB9722481D0 (en) * | 1997-10-25 | 1997-12-24 | Secr Defence | Expression system |
| US6407046B1 (en) * | 1998-09-03 | 2002-06-18 | Genencor International, Inc. | Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same |
| US6187732B1 (en) * | 1998-09-03 | 2001-02-13 | Genencor International, Inc. | Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same |
| EP1117808B1 (en) * | 1998-10-06 | 2004-12-29 | Mark Aaron Emalfarb | Transformation system in the field of filamentous fungal hosts: in chrysosporium |
| DE60036676T2 (de) * | 1999-10-29 | 2008-02-07 | Pfizer Inc. | G-protein-gekoppelte rezeptor-ähnliche proteine |
| DE10038722C2 (de) | 2000-08-09 | 2002-07-25 | Genethor Gmbh | Verfahren zur Reduzierung von spezifischen Immunreaktionen |
| EP2305801B1 (en) * | 2002-08-16 | 2017-07-05 | Danisco US Inc. | Novel variant hyprocrea jecorina CBH1 cellulases |
| WO2005001036A2 (en) | 2003-05-29 | 2005-01-06 | Genencor International, Inc. | Novel trichoderma genes |
| US8097445B2 (en) | 2004-03-25 | 2012-01-17 | Danisco Us Inc. | Exo-endo cellulase fusion protein |
| US20080229514A1 (en) | 2007-03-22 | 2008-09-25 | Poulose Ayrookaran J | Cleaning compositions comprising transglucosidase |
| WO2008148131A1 (en) | 2007-05-31 | 2008-12-04 | Novozymes, Inc. | Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same |
| US20110053218A1 (en) * | 2007-12-19 | 2011-03-03 | Applimex Systems Pty Ltd. | Multiple promoter platform for protein production |
| CN102245628B (zh) * | 2008-12-16 | 2014-05-28 | 荷兰联合利华有限公司 | 从溶液中提取疏水蛋白的方法 |
| US8993294B2 (en) | 2009-08-10 | 2015-03-31 | Danisco Us Inc. | Cross-flow membrane filtration-based process for protein recovery |
| US20120252066A1 (en) | 2011-03-29 | 2012-10-04 | Heng Meng H | Methods of foam control |
| EP2697245A4 (en) | 2011-04-15 | 2014-11-05 | Danisco Us Inc | METHODS OF PURIFYING HYDROPHOBIN |
-
2011
- 2011-10-19 EP EP11835033.9A patent/EP2630249B1/en active Active
- 2011-10-19 US US13/276,467 patent/US20120107872A1/en not_active Abandoned
- 2011-10-19 AU AU2011317171A patent/AU2011317171B2/en not_active Ceased
- 2011-10-19 CN CN201180050749.1A patent/CN103562402A/zh active Pending
- 2011-10-19 BR BR112013009532A patent/BR112013009532A2/pt not_active IP Right Cessation
- 2011-10-19 MX MX2013004203A patent/MX2013004203A/es not_active Application Discontinuation
- 2011-10-19 DK DK11835033.9T patent/DK2630249T3/da active
- 2011-10-19 CA CA2815070A patent/CA2815070A1/en not_active Abandoned
- 2011-10-19 RU RU2013122800/10A patent/RU2013122800A/ru not_active Application Discontinuation
- 2011-10-19 JP JP2013535026A patent/JP2014503185A/ja active Pending
- 2011-10-19 KR KR1020137009740A patent/KR20130132416A/ko not_active Application Discontinuation
- 2011-10-19 WO PCT/US2011/056810 patent/WO2012054554A2/en active Application Filing
-
2015
- 2015-08-20 US US14/831,763 patent/US9828591B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| BR112013009532A2 (pt) | 2016-07-19 |
| JP2014503185A (ja) | 2014-02-13 |
| AU2011317171A1 (en) | 2013-03-14 |
| RU2013122800A (ru) | 2014-11-27 |
| US20160040144A1 (en) | 2016-02-11 |
| CN103562402A (zh) | 2014-02-05 |
| US9828591B2 (en) | 2017-11-28 |
| MX2013004203A (es) | 2013-06-05 |
| KR20130132416A (ko) | 2013-12-04 |
| WO2012054554A2 (en) | 2012-04-26 |
| EP2630249A2 (en) | 2013-08-28 |
| EP2630249B1 (en) | 2018-09-05 |
| WO2012054554A3 (en) | 2013-10-24 |
| CA2815070A1 (en) | 2012-04-26 |
| EP2630249A4 (en) | 2014-12-03 |
| US20120107872A1 (en) | 2012-05-03 |
| AU2011317171B2 (en) | 2014-09-25 |
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