WO2012054554A3 - Thermostable trichoderma cellulase - Google Patents

Thermostable trichoderma cellulase Download PDF

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Publication number
WO2012054554A3
WO2012054554A3 PCT/US2011/056810 US2011056810W WO2012054554A3 WO 2012054554 A3 WO2012054554 A3 WO 2012054554A3 US 2011056810 W US2011056810 W US 2011056810W WO 2012054554 A3 WO2012054554 A3 WO 2012054554A3
Authority
WO
WIPO (PCT)
Prior art keywords
thermostable
trichoderma cellulase
egv
expression
trichoderma
Prior art date
Application number
PCT/US2011/056810
Other languages
French (fr)
Other versions
WO2012054554A2 (en
Inventor
Andrei Miasnikov
Michael Schelle
Michael Ward
Original Assignee
Danisco Us Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc. filed Critical Danisco Us Inc.
Priority to JP2013535026A priority Critical patent/JP2014503185A/en
Priority to DK11835033.9T priority patent/DK2630249T3/en
Priority to BR112013009532A priority patent/BR112013009532A2/en
Priority to CA2815070A priority patent/CA2815070A1/en
Priority to EP11835033.9A priority patent/EP2630249B1/en
Priority to AU2011317171A priority patent/AU2011317171B2/en
Priority to RU2013122800/10A priority patent/RU2013122800A/en
Priority to MX2013004203A priority patent/MX2013004203A/en
Priority to CN201180050749.1A priority patent/CN103562402A/en
Priority to KR1020137009740A priority patent/KR20130132416A/en
Publication of WO2012054554A2 publication Critical patent/WO2012054554A2/en
Publication of WO2012054554A3 publication Critical patent/WO2012054554A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Described are compositions and methods relating to the thermostable fungal cellulase enzyme, EGV, and Trichoderma host cells having a modification comprising or consisting essentially of disruption or deletion of nucleotide(s) for expression of this cellulose, whereby EGV expression is prevented.
PCT/US2011/056810 2010-10-20 2011-10-19 Thermostable trichoderma cellulase WO2012054554A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
JP2013535026A JP2014503185A (en) 2010-10-20 2011-10-19 Thermostable TRICHODERMA cellulase
DK11835033.9T DK2630249T3 (en) 2010-10-20 2011-10-19 THERMOSTABLE TRICHODERMA CELLULASE
BR112013009532A BR112013009532A2 (en) 2010-10-20 2011-10-19 thermostable tricoderma cellulase
CA2815070A CA2815070A1 (en) 2010-10-20 2011-10-19 Thermostable trichoderma cellulase
EP11835033.9A EP2630249B1 (en) 2010-10-20 2011-10-19 Thermostable trichoderma cellulase
AU2011317171A AU2011317171B2 (en) 2010-10-20 2011-10-19 Thermostable Trichoderma cellulase
RU2013122800/10A RU2013122800A (en) 2010-10-20 2011-10-19 THERMOSTABLE CELLULASE FROM TRICHODERMA
MX2013004203A MX2013004203A (en) 2010-10-20 2011-10-19 Thermostable trichoderma cellulase.
CN201180050749.1A CN103562402A (en) 2010-10-20 2011-10-19 Thermostable trichoderma cellulase
KR1020137009740A KR20130132416A (en) 2010-10-20 2011-10-19 Thermostable trichoderma cellulase

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US39494610P 2010-10-20 2010-10-20
US61/394,946 2010-10-20
EP10188285.0 2010-10-20
EP10188285 2010-10-20

Publications (2)

Publication Number Publication Date
WO2012054554A2 WO2012054554A2 (en) 2012-04-26
WO2012054554A3 true WO2012054554A3 (en) 2013-10-24

Family

ID=44022886

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/056810 WO2012054554A2 (en) 2010-10-20 2011-10-19 Thermostable trichoderma cellulase

Country Status (12)

Country Link
US (2) US20120107872A1 (en)
EP (1) EP2630249B1 (en)
JP (1) JP2014503185A (en)
KR (1) KR20130132416A (en)
CN (1) CN103562402A (en)
AU (1) AU2011317171B2 (en)
BR (1) BR112013009532A2 (en)
CA (1) CA2815070A1 (en)
DK (1) DK2630249T3 (en)
MX (1) MX2013004203A (en)
RU (1) RU2013122800A (en)
WO (1) WO2012054554A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013177153A1 (en) * 2012-05-21 2013-11-28 Danisco Us Inc. Trichoderma hydrophobin production
DK3030661T3 (en) 2013-08-08 2019-06-11 Danisco Us Inc INCREASE OF A TRANSGENS EXPRESSION IN EUCARYOTIC CELLS BY REDUCTION OF RNA INTERFERENCE
WO2015051121A1 (en) 2013-10-02 2015-04-09 E. I. Du Pont De Nemours And Company Hydrophobin composition and process for treating surfaces
CN106011114B (en) * 2016-07-28 2019-05-24 江南大学 A kind of fermentation liquor treatment method improving Pullulanase stability
CN109790510B (en) * 2016-10-04 2024-04-12 丹尼斯科美国公司 Protein production in filamentous fungal cells in the absence of an inducing substrate
KR102155046B1 (en) * 2018-06-18 2020-09-11 국민대학교산학협력단 Novel Trichoderma sp. KMF006 strain producing cellulase with high activity
CN111944790B (en) * 2020-07-01 2022-09-09 深圳润康生态环境股份有限公司 Neutral protease gene, neutral protease, preparation method and application thereof
CN112899206B (en) * 2021-04-12 2022-07-26 西北农林科技大学 Bacillus for producing chitinase and indoleacetic acid and application and method thereof
WO2023039358A1 (en) 2021-09-09 2023-03-16 Dupont Nutrition Biosciences Aps Over expression of foldases and chaperones improves protein production

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020045234A1 (en) * 1997-10-25 2002-04-18 Squirrell David J Expression system
US20020165114A1 (en) * 1998-09-03 2002-11-07 Timothy Fowler Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
US20030149242A1 (en) * 1999-10-29 2003-08-07 Pfizer Inc. Novel polypeptide
US20040002136A1 (en) * 1998-10-06 2004-01-01 Emalfarb Mark Aaron Transformation system in the field of filamentous fungal hosts
US20070173431A1 (en) * 2002-08-16 2007-07-26 Anthony Day Novel variant hypocrea jercorina CBH1 cellulases
WO2009076709A1 (en) * 2007-12-19 2009-06-25 Applimex Systems Pty Ltd Multiple promoter platform for protein production
US20100151525A1 (en) * 2008-12-16 2010-06-17 Conopco, Inc., D/B/A Unilever Method for extracting hydrophobin from a solution

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5650322A (en) 1990-10-05 1997-07-22 Genencor International, Inc. Methods for stonewashing fabrics using endoglucanases
ES2105925B1 (en) 1993-05-12 1998-06-01 Blas Burguet Rodrigo DEVICE FOR CUTTING BREAD TRANSPORTED IN TROLLEYS.
US6017870A (en) * 1996-10-09 2000-01-25 Genencor International, Inc. Purified cellulase and method of producing
US6187732B1 (en) * 1998-09-03 2001-02-13 Genencor International, Inc. Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
DE10038722C2 (en) 2000-08-09 2002-07-25 Genethor Gmbh Procedure for the reduction of specific immune reactions
DK1627049T3 (en) 2003-05-29 2010-05-31 Genencor Int New Trichoderma Genes
US8097445B2 (en) 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
US20080229514A1 (en) 2007-03-22 2008-09-25 Poulose Ayrookaran J Cleaning compositions comprising transglucosidase
BRPI0812035A2 (en) 2007-05-31 2014-10-14 Novozymes Inc ISOLA POLYPEPTIDE, ISOLATED POLYNUCLEOTIDE, NUCLEIC ACID CONSTRUCTION, RECOMBINANT EXPRESSION VECTOR, RECOMBINANT HOST CELL, METHODS FOR PRODUCING A POLYPETITE FOR A CIRCLE INPUT AND A CIRCLE INPUT CONVERTING MATERIAL CONTAINING CELLULOSE AND TO PRODUCE A FERMENTATION PRODUCT, MUTING CELL, PLANT, PART OF PLANT OR TRANSGENIC PLANT CELL, AND DOUBLE TAPE INHIBITOR RNA MOLECLE
AR077838A1 (en) 2009-08-10 2011-09-28 Danisco Us Inc PROCESS BASED ON THE TANGENTIAL FILTRATION IN MEMBRANE FOR THE RECOVERY OF PROTEINS
US20120252066A1 (en) 2011-03-29 2012-10-04 Heng Meng H Methods of foam control
JP2014510796A (en) 2011-04-15 2014-05-01 ダニスコ・ユーエス・インク Method for purifying hydrophobin

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020045234A1 (en) * 1997-10-25 2002-04-18 Squirrell David J Expression system
US20020165114A1 (en) * 1998-09-03 2002-11-07 Timothy Fowler Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
US20040002136A1 (en) * 1998-10-06 2004-01-01 Emalfarb Mark Aaron Transformation system in the field of filamentous fungal hosts
US20030149242A1 (en) * 1999-10-29 2003-08-07 Pfizer Inc. Novel polypeptide
US20070173431A1 (en) * 2002-08-16 2007-07-26 Anthony Day Novel variant hypocrea jercorina CBH1 cellulases
WO2009076709A1 (en) * 2007-12-19 2009-06-25 Applimex Systems Pty Ltd Multiple promoter platform for protein production
US20100151525A1 (en) * 2008-12-16 2010-06-17 Conopco, Inc., D/B/A Unilever Method for extracting hydrophobin from a solution

Also Published As

Publication number Publication date
AU2011317171B2 (en) 2014-09-25
WO2012054554A2 (en) 2012-04-26
BR112013009532A2 (en) 2016-07-19
US9828591B2 (en) 2017-11-28
EP2630249B1 (en) 2018-09-05
RU2013122800A (en) 2014-11-27
MX2013004203A (en) 2013-06-05
US20160040144A1 (en) 2016-02-11
EP2630249A4 (en) 2014-12-03
CA2815070A1 (en) 2012-04-26
DK2630249T3 (en) 2018-12-17
EP2630249A2 (en) 2013-08-28
KR20130132416A (en) 2013-12-04
AU2011317171A1 (en) 2013-03-14
US20120107872A1 (en) 2012-05-03
CN103562402A (en) 2014-02-05
JP2014503185A (en) 2014-02-13

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