DK2547782T3 - METHODS, KITS AND COMPOSITIONS FOR MRSA DETECTION - Google Patents
METHODS, KITS AND COMPOSITIONS FOR MRSA DETECTION Download PDFInfo
- Publication number
- DK2547782T3 DK2547782T3 DK11757080.4T DK11757080T DK2547782T3 DK 2547782 T3 DK2547782 T3 DK 2547782T3 DK 11757080 T DK11757080 T DK 11757080T DK 2547782 T3 DK2547782 T3 DK 2547782T3
- Authority
- DK
- Denmark
- Prior art keywords
- seq
- gene
- tuf
- aureus
- cns
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 53
- 238000001514 detection method Methods 0.000 title claims description 15
- 239000000203 mixture Substances 0.000 title description 24
- 239000000523 sample Substances 0.000 claims description 167
- 108090000623 proteins and genes Proteins 0.000 claims description 105
- 238000007837 multiplex assay Methods 0.000 claims description 60
- 241000894007 species Species 0.000 claims description 60
- 108091034117 Oligonucleotide Proteins 0.000 claims description 55
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 claims description 41
- 229960003085 meticillin Drugs 0.000 claims description 41
- 230000014509 gene expression Effects 0.000 claims description 35
- 238000003753 real-time PCR Methods 0.000 claims description 32
- 101150008979 mecA gene Proteins 0.000 claims description 26
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 24
- 210000002615 epidermis Anatomy 0.000 claims description 22
- 241000894006 Bacteria Species 0.000 claims description 18
- 238000003757 reverse transcription PCR Methods 0.000 claims description 18
- 230000002441 reversible effect Effects 0.000 claims description 14
- 229930182555 Penicillin Natural products 0.000 claims description 12
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 12
- 241000191940 Staphylococcus Species 0.000 claims description 12
- 229940049954 penicillin Drugs 0.000 claims description 12
- 238000011529 RT qPCR Methods 0.000 claims description 7
- 238000012216 screening Methods 0.000 claims description 5
- 230000007613 environmental effect Effects 0.000 claims description 3
- 239000012472 biological sample Substances 0.000 claims description 2
- 206010041925 Staphylococcal infections Diseases 0.000 claims 6
- 208000015688 methicillin-resistant staphylococcus aureus infectious disease Diseases 0.000 claims 6
- 108700026220 vif Genes Proteins 0.000 claims 4
- 238000003556 assay Methods 0.000 description 188
- 239000013615 primer Substances 0.000 description 64
- 108020004414 DNA Proteins 0.000 description 54
- 150000007523 nucleic acids Chemical group 0.000 description 46
- 108700028369 Alleles Proteins 0.000 description 41
- 241000191963 Staphylococcus epidermidis Species 0.000 description 38
- 238000003199 nucleic acid amplification method Methods 0.000 description 37
- 230000003321 amplification Effects 0.000 description 36
- 238000006243 chemical reaction Methods 0.000 description 36
- 108020004707 nucleic acids Proteins 0.000 description 35
- 102000039446 nucleic acids Human genes 0.000 description 35
- 101150099542 tuf gene Proteins 0.000 description 33
- 101150022703 femA gene Proteins 0.000 description 32
- 238000003752 polymerase chain reaction Methods 0.000 description 27
- 241000191967 Staphylococcus aureus Species 0.000 description 18
- 238000004458 analytical method Methods 0.000 description 18
- 239000002987 primer (paints) Substances 0.000 description 18
- 238000012163 sequencing technique Methods 0.000 description 17
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 13
- 208000015181 infectious disease Diseases 0.000 description 13
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 12
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 11
- 239000002751 oligonucleotide probe Substances 0.000 description 11
- 239000000758 substrate Substances 0.000 description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 9
- 239000002773 nucleotide Substances 0.000 description 9
- 125000003729 nucleotide group Chemical group 0.000 description 9
- 102000004169 proteins and genes Human genes 0.000 description 9
- 239000000975 dye Substances 0.000 description 8
- 238000009396 hybridization Methods 0.000 description 8
- 239000000090 biomarker Substances 0.000 description 7
- 239000007850 fluorescent dye Substances 0.000 description 7
- -1 ny Species 0.000 description 7
- 230000003287 optical effect Effects 0.000 description 7
- WCKQPPQRFNHPRJ-UHFFFAOYSA-N 4-[[4-(dimethylamino)phenyl]diazenyl]benzoic acid Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=C(C(O)=O)C=C1 WCKQPPQRFNHPRJ-UHFFFAOYSA-N 0.000 description 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 description 6
- 229960002685 biotin Drugs 0.000 description 6
- 235000020958 biotin Nutrition 0.000 description 6
- 239000011616 biotin Substances 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 239000012634 fragment Substances 0.000 description 6
- 210000003491 skin Anatomy 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 5
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 241001147736 Staphylococcus capitis Species 0.000 description 5
- 241000191984 Staphylococcus haemolyticus Species 0.000 description 5
- 241000192087 Staphylococcus hominis Species 0.000 description 5
- 241001134656 Staphylococcus lugdunensis Species 0.000 description 5
- 230000027455 binding Effects 0.000 description 5
- 101150015862 epi gene Proteins 0.000 description 5
- 238000003205 genotyping method Methods 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 241000191978 Staphylococcus simulans Species 0.000 description 4
- 239000011324 bead Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 101150100619 nuc gene Proteins 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000011002 quantification Methods 0.000 description 4
- 230000002285 radioactive effect Effects 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- LYOKOJQBUZRTMX-UHFFFAOYSA-N 1,3-bis[[1,1,1,3,3,3-hexafluoro-2-(trifluoromethyl)propan-2-yl]oxy]-2,2-bis[[1,1,1,3,3,3-hexafluoro-2-(trifluoromethyl)propan-2-yl]oxymethyl]propane Chemical compound FC(F)(F)C(C(F)(F)F)(C(F)(F)F)OCC(COC(C(F)(F)F)(C(F)(F)F)C(F)(F)F)(COC(C(F)(F)F)(C(F)(F)F)C(F)(F)F)COC(C(F)(F)F)(C(F)(F)F)C(F)(F)F LYOKOJQBUZRTMX-UHFFFAOYSA-N 0.000 description 3
- SJQRQOKXQKVJGJ-UHFFFAOYSA-N 5-(2-aminoethylamino)naphthalene-1-sulfonic acid Chemical compound C1=CC=C2C(NCCN)=CC=CC2=C1S(O)(=O)=O SJQRQOKXQKVJGJ-UHFFFAOYSA-N 0.000 description 3
- NJYVEMPWNAYQQN-UHFFFAOYSA-N 5-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C21OC(=O)C1=CC(C(=O)O)=CC=C21 NJYVEMPWNAYQQN-UHFFFAOYSA-N 0.000 description 3
- 208000035143 Bacterial infection Diseases 0.000 description 3
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 3
- 108010065152 Coagulase Proteins 0.000 description 3
- 238000000018 DNA microarray Methods 0.000 description 3
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- 241000295644 Staphylococcaceae Species 0.000 description 3
- 241001147691 Staphylococcus saprophyticus Species 0.000 description 3
- 108010090804 Streptavidin Proteins 0.000 description 3
- 150000001251 acridines Chemical class 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 208000022362 bacterial infectious disease Diseases 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000000969 carrier Substances 0.000 description 3
- 239000013522 chelant Substances 0.000 description 3
- 150000001841 cholesterols Chemical class 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 3
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 3
- 229960001484 edetic acid Drugs 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000010348 incorporation Methods 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 150000003194 psoralenes Chemical class 0.000 description 3
- 238000010791 quenching Methods 0.000 description 3
- 230000000171 quenching effect Effects 0.000 description 3
- 230000005855 radiation Effects 0.000 description 3
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- YBBRCQOCSYXUOC-UHFFFAOYSA-N sulfuryl dichloride Chemical compound ClS(Cl)(=O)=O YBBRCQOCSYXUOC-UHFFFAOYSA-N 0.000 description 3
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 3
- 230000002103 transcriptional effect Effects 0.000 description 3
- 238000001712 DNA sequencing Methods 0.000 description 2
- 206010061598 Immunodeficiency Diseases 0.000 description 2
- 102100034343 Integrase Human genes 0.000 description 2
- 208000037942 Methicillin-resistant Staphylococcus aureus infection Diseases 0.000 description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 238000010222 PCR analysis Methods 0.000 description 2
- 238000002944 PCR assay Methods 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 2
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- 206010057190 Respiratory tract infections Diseases 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 239000002313 adhesive film Substances 0.000 description 2
- 238000001574 biopsy Methods 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000009977 dual effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 238000007834 ligase chain reaction Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 238000002493 microarray Methods 0.000 description 2
- 238000010208 microarray analysis Methods 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 238000012175 pyrosequencing Methods 0.000 description 2
- 230000000241 respiratory effect Effects 0.000 description 2
- 230000009870 specific binding Effects 0.000 description 2
- 210000003802 sputum Anatomy 0.000 description 2
- 208000024794 sputum Diseases 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- OAKPWEUQDVLTCN-NKWVEPMBSA-N 2',3'-Dideoxyadenosine-5-triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1CC[C@@H](CO[P@@](O)(=O)O[P@](O)(=O)OP(O)(O)=O)O1 OAKPWEUQDVLTCN-NKWVEPMBSA-N 0.000 description 1
- 125000000022 2-aminoethyl group Chemical group [H]C([*])([H])C([H])([H])N([H])[H] 0.000 description 1
- 208000030507 AIDS Diseases 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 208000031729 Bacteremia Diseases 0.000 description 1
- 208000034309 Bacterial disease carrier Diseases 0.000 description 1
- 108091032955 Bacterial small RNA Proteins 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 206010008479 Chest Pain Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241001137251 Corvidae Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 208000010201 Exanthema Diseases 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 108020005196 Mitochondrial DNA Proteins 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 208000000112 Myalgia Diseases 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 208000002151 Pleural effusion Diseases 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 238000010240 RT-PCR analysis Methods 0.000 description 1
- 108091028664 Ribonucleotide Proteins 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 108020004459 Small interfering RNA Proteins 0.000 description 1
- 206010062255 Soft tissue infection Diseases 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 241001147686 Staphylococcus arlettae Species 0.000 description 1
- 241001033898 Staphylococcus equorum Species 0.000 description 1
- 241000192086 Staphylococcus warneri Species 0.000 description 1
- 241000191973 Staphylococcus xylosus Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 102000004523 Sulfate Adenylyltransferase Human genes 0.000 description 1
- 108010022348 Sulfate adenylyltransferase Proteins 0.000 description 1
- 208000033809 Suppuration Diseases 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- HDRRAMINWIWTNU-NTSWFWBYSA-N [[(2s,5r)-5-(2-amino-6-oxo-3h-purin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@H]1CC[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HDRRAMINWIWTNU-NTSWFWBYSA-N 0.000 description 1
- ARLKCWCREKRROD-POYBYMJQSA-N [[(2s,5r)-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)CC1 ARLKCWCREKRROD-POYBYMJQSA-N 0.000 description 1
- 206010000269 abscess Diseases 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000003570 air Substances 0.000 description 1
- 210000004381 amniotic fluid Anatomy 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000000386 athletic effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000009640 blood culture Methods 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- URGJWIFLBWJRMF-JGVFFNPUSA-N ddTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)CC1 URGJWIFLBWJRMF-JGVFFNPUSA-N 0.000 description 1
- 230000037430 deletion Effects 0.000 description 1
- 238000012217 deletion Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000005546 dideoxynucleotide Substances 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000006471 dimerization reaction Methods 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 210000004696 endometrium Anatomy 0.000 description 1
- 238000007824 enzymatic assay Methods 0.000 description 1
- 210000003238 esophagus Anatomy 0.000 description 1
- 201000005884 exanthem Diseases 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 210000003754 fetus Anatomy 0.000 description 1
- LIYGYAHYXQDGEP-UHFFFAOYSA-N firefly oxyluciferin Natural products Oc1csc(n1)-c1nc2ccc(O)cc2s1 LIYGYAHYXQDGEP-UHFFFAOYSA-N 0.000 description 1
- 238000000684 flow cytometry Methods 0.000 description 1
- 238000005755 formation reaction Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 231100000221 frame shift mutation induction Toxicity 0.000 description 1
- 230000037433 frameshift Effects 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000013412 genome amplification Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 238000007850 in situ PCR Methods 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000012678 infectious agent Substances 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000004880 lymph fluid Anatomy 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 238000007403 mPCR Methods 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 238000013178 mathematical model Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000693 micelle Substances 0.000 description 1
- 239000011325 microbead Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 210000003739 neck Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 238000007481 next generation sequencing Methods 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 238000002966 oligonucleotide array Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- JJVOROULKOMTKG-UHFFFAOYSA-N oxidized Photinus luciferin Chemical compound S1C2=CC(O)=CC=C2N=C1C1=NC(=O)CS1 JJVOROULKOMTKG-UHFFFAOYSA-N 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- 235000015108 pies Nutrition 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
- 230000004850 protein–protein interaction Effects 0.000 description 1
- 238000003762 quantitative reverse transcription PCR Methods 0.000 description 1
- 230000005258 radioactive decay Effects 0.000 description 1
- 206010037844 rash Diseases 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 239000002336 ribonucleotide Substances 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000007480 sanger sequencing Methods 0.000 description 1
- 238000007423 screening assay Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 239000012056 semi-solid material Substances 0.000 description 1
- 210000004911 serous fluid Anatomy 0.000 description 1
- 208000013220 shortness of breath Diseases 0.000 description 1
- 150000004756 silanes Chemical class 0.000 description 1
- 230000037432 silent mutation Effects 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 206010040872 skin infection Diseases 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 108010068698 spleen exonuclease Proteins 0.000 description 1
- 229940037649 staphylococcus haemolyticus Drugs 0.000 description 1
- 229940037648 staphylococcus simulans Drugs 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 230000009452 underexpressoin Effects 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US31566410P | 2010-03-19 | 2010-03-19 | |
| US201161432511P | 2011-01-13 | 2011-01-13 | |
| PCT/US2011/029043 WO2011116313A1 (en) | 2010-03-19 | 2011-03-18 | Methods, kits and compositions for detection of mrsa |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2547782T3 true DK2547782T3 (en) | 2018-10-08 |
Family
ID=44649626
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK11757080.4T DK2547782T3 (en) | 2010-03-19 | 2011-03-18 | METHODS, KITS AND COMPOSITIONS FOR MRSA DETECTION |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10100368B2 (enExample) |
| EP (1) | EP2547782B8 (enExample) |
| JP (1) | JP6320041B2 (enExample) |
| AU (1) | AU2011227110B2 (enExample) |
| BR (1) | BR112012023698B1 (enExample) |
| CA (1) | CA2793660C (enExample) |
| DK (1) | DK2547782T3 (enExample) |
| ES (1) | ES2689953T3 (enExample) |
| WO (1) | WO2011116313A1 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014137906A1 (en) * | 2013-03-05 | 2014-09-12 | Intelligent Medical Devices, Inc. | Optimized probes and primers and methods of using same for the detection, screening, isolation and sequencing of mrsa, mssa, staphylococcus markers, and the antibiotic resistance gene mec a |
| US20140274770A1 (en) | 2013-03-13 | 2014-09-18 | Quidel Corporation | Pcr assays and reagents for molecular detection of infectious agents |
| PL228161B1 (pl) | 2013-05-21 | 2018-02-28 | Univ Jagiellonski | Sposób jednoczesnej detekcji bakterii i grzybów w preparacie biologicznym metoda PCR oraz zestaw do detekcji bakterii i grzybów w preparacie biologicznym metoda PCR |
| CN103421898B (zh) * | 2013-08-08 | 2015-08-12 | 广东出入境检验检疫局检验检疫技术中心 | 耐甲氧西林金黄色葡萄球菌三重实时荧光pcr检测引物、探针、检测试剂盒和检测方法 |
| US10407739B2 (en) | 2013-09-23 | 2019-09-10 | Quest Diagnostics Investments Incorporated | Detection of methicillin-resistant Staphylococcus aureus in biological samples |
| WO2015053061A1 (ja) * | 2013-10-08 | 2015-04-16 | 東洋紡株式会社 | メチシリン耐性遺伝子の検出方法および黄色ブドウ球菌の検出方法 |
| JP6225623B2 (ja) * | 2013-10-08 | 2017-11-08 | 東洋紡株式会社 | メチシリン耐性遺伝子の検出方法 |
| JP6343899B2 (ja) * | 2013-10-08 | 2018-06-20 | 東洋紡株式会社 | 黄色ブドウ球菌の検出方法 |
| WO2016168844A1 (en) | 2015-04-17 | 2016-10-20 | The Translational Genomics Research Institute | Quality assessment of circulating cell-free dna using multiplexed droplet digital pcr |
| PL235777B1 (pl) | 2015-07-10 | 2020-10-19 | Univ Jagiellonski | Startery, sposób i zestaw diagnostyczny do diagnozowania sepsy |
| EP4219734A1 (en) * | 2015-11-04 | 2023-08-02 | The Translational Genomics Research Institute | Systems and methods of diagnosing and characterizing infections |
| EP3805407A4 (en) * | 2018-05-25 | 2021-08-11 | Mitsubishi Chemical Corporation | TARGET NUCLEIC ACID QUANTIFICATION PROCESS |
| US20210373015A1 (en) * | 2018-10-23 | 2021-12-02 | Myriad Applied Technologies, Inc. | Kits and methods for real-time mulitplex detection and identification of pathogens |
| EP3936627A1 (en) * | 2020-07-06 | 2022-01-12 | Beiersdorf AG | Novel oligonucleotides for detecting staphylococcus |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5716784A (en) | 1996-02-05 | 1998-02-10 | The Perkin-Elmer Corporation | Fluorescence detection assay for homogeneous PCR hybridization systems |
| US7060458B1 (en) * | 1997-08-14 | 2006-06-13 | Wyeth | Nucleic acid and amino acid sequences relating to Staphylococcus epidermidis for diagnostics and therapeutics |
| CA2388461C (en) | 1999-09-28 | 2013-06-11 | Infectio Diagnostic (I.D.I.) Inc. | Highly conserved genes and their use to generate probes and primers for the detection of microorganisms |
| WO2001083821A2 (en) * | 2000-05-01 | 2001-11-08 | Gen-Probe Incorporated | Polynucleotide probes for detection and quantitation of candida species |
| DE60217837T2 (de) * | 2001-03-15 | 2007-10-25 | Jacques Schrenzel | Nachweis von methicillin-resistenten staphylococcus aureus bakterien (mrsa) |
| CA2528025A1 (en) | 2003-06-05 | 2005-02-17 | Wyeth | Nucleic acid arrays for detecting multiple strains of a non-viral species |
| WO2009037575A2 (en) * | 2007-04-19 | 2009-03-26 | Uti Limited Partnership | Multiplex pcr assay for identification of usa300 and usa400 community-associated methicillin resistant staphylococcal aureus strains |
| WO2009018000A1 (en) * | 2007-07-31 | 2009-02-05 | Quest Diagnostics Investments Incorporated | Detection of methicillin-resistant and methicillin-sensitive staphylococcus aureus in biological samples |
| AU2008345600B2 (en) | 2007-12-21 | 2014-07-24 | Gen-Probe Incorporated | Detection of antibiotic-resistant microorganisms |
-
2011
- 2011-03-18 WO PCT/US2011/029043 patent/WO2011116313A1/en not_active Ceased
- 2011-03-18 US US13/051,755 patent/US10100368B2/en active Active
- 2011-03-18 JP JP2013500236A patent/JP6320041B2/ja active Active
- 2011-03-18 EP EP11757080.4A patent/EP2547782B8/en active Active
- 2011-03-18 AU AU2011227110A patent/AU2011227110B2/en active Active
- 2011-03-18 DK DK11757080.4T patent/DK2547782T3/en active
- 2011-03-18 BR BR112012023698-9A patent/BR112012023698B1/pt active IP Right Grant
- 2011-03-18 CA CA2793660A patent/CA2793660C/en active Active
- 2011-03-18 ES ES11757080.4T patent/ES2689953T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| AU2011227110B2 (en) | 2016-01-28 |
| US20110312504A1 (en) | 2011-12-22 |
| JP6320041B2 (ja) | 2018-05-09 |
| BR112012023698A2 (pt) | 2015-11-03 |
| CA2793660A1 (en) | 2011-09-22 |
| EP2547782B1 (en) | 2018-08-01 |
| US10100368B2 (en) | 2018-10-16 |
| EP2547782B8 (en) | 2018-11-14 |
| AU2011227110A1 (en) | 2012-10-25 |
| CA2793660C (en) | 2020-12-22 |
| EP2547782A4 (en) | 2013-10-30 |
| JP2013535951A (ja) | 2013-09-19 |
| BR112012023698B1 (pt) | 2019-10-15 |
| WO2011116313A1 (en) | 2011-09-22 |
| ES2689953T3 (es) | 2018-11-16 |
| EP2547782A1 (en) | 2013-01-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK2547782T3 (en) | METHODS, KITS AND COMPOSITIONS FOR MRSA DETECTION | |
| Gow et al. | Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods | |
| US9340837B2 (en) | Methods and kits useful in the differentiation of Burkholderia species | |
| Rostami et al. | High resolution melting technique for molecular epidemiological studies of cystic echinococcosis: differentiating G1, G3, and G6 genotypes of Echinococcus granulosus sensu lato | |
| CN113481311B (zh) | 用于鉴定布鲁氏菌疫苗株m5的snp分子标记及其应用 | |
| Kılıç et al. | Brucella melitensis and Brucella abortus genotyping via real-time PCR targeting 21 variable genome loci | |
| CN103333903A (zh) | 检测幽门螺杆菌的靶序列、引物和探针及其试剂盒 | |
| KR20130077207A (ko) | 돼지의 다산 개체 선발을 위한 ｓｎｐ 마커와 이를 이용한 평가방법 | |
| US20150031576A1 (en) | Real time pcr detection of m. tuberculosis resistant/susceptible to rifampicin and/or isoniazid | |
| US9340836B2 (en) | Allelic discrimination assays for MRSA strains | |
| CN116064842B (zh) | 一种用于降解检材推断的生物地理祖先DIPs和性别鉴定的复合扩增盒 | |
| CN104087661A (zh) | TaqMan实时荧光定量PCR检测杆菌样巴尔通体的方法 | |
| US9914978B2 (en) | Methods, kits, and compositions useful in selecting an antibiotic to treat MRSA | |
| JP2023020631A (ja) | 全身性エリテマトーデスを検査する方法 | |
| KR101106963B1 (ko) | Ｐ１ 유전자를 이용한 마이코플라스마 뉴모니아균 검출용 ｐｃｒ 프라이머와 프로브 및 이를 이용한 실시간 ｐｃｒ 방법 | |
| US9970066B2 (en) | Method of detecting Coccidioides species | |
| US9404161B2 (en) | Method of detecting and quantifying coccidioides species | |
| JP7461624B2 (ja) | Ｂ型肝炎ワクチンに対する免疫応答性の遺伝的要因を検出する方法及びキット | |
| CN110564877B (zh) | 以转酮醇酶基因为靶标检测绵羊肺炎支原体的组合物、试剂盒和方法 | |
| CN120945077A (zh) | 一种用于检测水生拉恩菌cpa-lfd方法及检测试剂盒 | |
| Mahony et al. | Improving bovine respiratory disease control through the characterisation of pathogen-host interactions | |
| TR2023010449A1 (tr) | Farkli örneklerdeki̇ brucella türleri̇ni̇ teşhi̇si̇ ve genoti̇plenmesi̇ i̇çi̇n snp beli̇rteçleri̇ ve kombi̇nasyonlari | |
| CN120310933A (zh) | 用于检测克雷伯菌的引物探针组合 | |
| US9127321B2 (en) | Method of detecting Coccidioides species | |
| CN105441582A (zh) | 幽门螺杆菌定性和定量多重基因检测体系及其试剂盒和应用 |