DK2417153T3 - Filtrering af en væske, der omfatter et stressprotein fra en plante - Google Patents
Filtrering af en væske, der omfatter et stressprotein fra en plante Download PDFInfo
- Publication number
- DK2417153T3 DK2417153T3 DK10713337.3T DK10713337T DK2417153T3 DK 2417153 T3 DK2417153 T3 DK 2417153T3 DK 10713337 T DK10713337 T DK 10713337T DK 2417153 T3 DK2417153 T3 DK 2417153T3
- Authority
- DK
- Denmark
- Prior art keywords
- stress protein
- liquid
- filtration
- filter aid
- plant
- Prior art date
Links
- 108010004889 Heat-Shock Proteins Proteins 0.000 title claims description 94
- 102000002812 Heat-Shock Proteins Human genes 0.000 title claims description 94
- 238000001914 filtration Methods 0.000 title claims description 83
- 239000007788 liquid Substances 0.000 title claims description 65
- 238000000034 method Methods 0.000 claims description 47
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 40
- 239000011707 mineral Substances 0.000 claims description 39
- 239000000843 powder Substances 0.000 claims description 39
- 241000196324 Embryophyta Species 0.000 claims description 33
- 239000000463 material Substances 0.000 claims description 33
- 239000000706 filtrate Substances 0.000 claims description 27
- 238000000926 separation method Methods 0.000 claims description 20
- 235000019804 chlorophyll Nutrition 0.000 claims description 15
- 229930002875 chlorophyll Natural products 0.000 claims description 14
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims description 14
- 239000002245 particle Substances 0.000 claims description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 11
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 11
- 238000005349 anion exchange Methods 0.000 claims description 10
- 238000004587 chromatography analysis Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 9
- 240000004658 Medicago sativa Species 0.000 claims description 8
- 238000001042 affinity chromatography Methods 0.000 claims description 8
- 238000000502 dialysis Methods 0.000 claims description 8
- 235000021466 carotenoid Nutrition 0.000 claims description 7
- 150000001747 carotenoids Chemical class 0.000 claims description 7
- 239000005909 Kieselgur Substances 0.000 claims description 6
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims description 6
- 238000000108 ultra-filtration Methods 0.000 claims description 6
- 101710163595 Chaperone protein DnaK Proteins 0.000 claims description 5
- 101710178376 Heat shock 70 kDa protein Proteins 0.000 claims description 5
- 101710152018 Heat shock cognate 70 kDa protein Proteins 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 5
- 238000011118 depth filtration Methods 0.000 claims description 5
- 239000010451 perlite Substances 0.000 claims description 5
- 235000019362 perlite Nutrition 0.000 claims description 5
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 claims description 4
- 239000012530 fluid Substances 0.000 claims description 4
- 102100034051 Heat shock protein HSP 90-alpha Human genes 0.000 claims description 3
- 238000001728 nano-filtration Methods 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 102100038222 60 kDa heat shock protein, mitochondrial Human genes 0.000 claims description 2
- 235000016068 Berberis vulgaris Nutrition 0.000 claims description 2
- 241000335053 Beta vulgaris Species 0.000 claims description 2
- 108010058432 Chaperonin 60 Proteins 0.000 claims description 2
- 241000871189 Chenopodiaceae Species 0.000 claims description 2
- 241000220485 Fabaceae Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 102000004447 HSP40 Heat-Shock Proteins Human genes 0.000 claims description 2
- 108010042283 HSP40 Heat-Shock Proteins Proteins 0.000 claims description 2
- 101710113864 Heat shock protein 90 Proteins 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 241000208292 Solanaceae Species 0.000 claims description 2
- 241000209140 Triticum Species 0.000 claims description 2
- 235000021307 Triticum Nutrition 0.000 claims description 2
- 240000008042 Zea mays Species 0.000 claims description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 2
- 235000021374 legumes Nutrition 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 239000000835 fiber Substances 0.000 claims 3
- 241000209504 Poaceae Species 0.000 claims 1
- 244000061456 Solanum tuberosum Species 0.000 claims 1
- 235000002595 Solanum tuberosum Nutrition 0.000 claims 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims 1
- 235000005822 corn Nutrition 0.000 claims 1
- 238000001223 reverse osmosis Methods 0.000 claims 1
- 238000001556 precipitation Methods 0.000 description 26
- 108090000623 proteins and genes Proteins 0.000 description 25
- 102000004169 proteins and genes Human genes 0.000 description 23
- 239000000872 buffer Substances 0.000 description 14
- 239000000047 product Substances 0.000 description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- 238000000746 purification Methods 0.000 description 12
- 239000011148 porous material Substances 0.000 description 11
- 238000005342 ion exchange Methods 0.000 description 10
- 150000001875 compounds Chemical class 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 7
- 229930006000 Sucrose Natural products 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 7
- 239000002244 precipitate Substances 0.000 description 7
- 239000005720 sucrose Substances 0.000 description 7
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 6
- 101000839464 Leishmania braziliensis Heat shock 70 kDa protein Proteins 0.000 description 6
- 230000000903 blocking effect Effects 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 5
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 5
- 239000001166 ammonium sulphate Substances 0.000 description 5
- 235000011130 ammonium sulphate Nutrition 0.000 description 5
- 235000011187 glycerol Nutrition 0.000 description 5
- 239000012535 impurity Substances 0.000 description 5
- 238000011282 treatment Methods 0.000 description 5
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 239000002537 cosmetic Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 239000004615 ingredient Substances 0.000 description 4
- 239000002773 nucleotide Substances 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- 230000035699 permeability Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 229920000936 Agarose Polymers 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 229920002684 Sepharose Polymers 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 108091000085 chlorophyll binding Proteins 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000012065 filter cake Substances 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 230000031700 light absorption Effects 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 238000005498 polishing Methods 0.000 description 3
- 238000003825 pressing Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 108091006112 ATPases Proteins 0.000 description 2
- 102000057290 Adenosine Triphosphatases Human genes 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 235000007319 Avena orientalis Nutrition 0.000 description 2
- 244000075850 Avena orientalis Species 0.000 description 2
- 235000007558 Avena sp Nutrition 0.000 description 2
- 101100507655 Canis lupus familiaris HSPA1 gene Proteins 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 235000010624 Medicago sativa Nutrition 0.000 description 2
- 108010006519 Molecular Chaperones Proteins 0.000 description 2
- 102000005431 Molecular Chaperones Human genes 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 229910052783 alkali metal Inorganic materials 0.000 description 2
- 150000001340 alkali metals Chemical class 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000002738 chelating agent Substances 0.000 description 2
- 239000003398 denaturant Substances 0.000 description 2
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000005187 foaming Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 2
- 239000000825 pharmaceutical preparation Substances 0.000 description 2
- 229940127557 pharmaceutical product Drugs 0.000 description 2
- 239000000419 plant extract Substances 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- WRDABNWSWOHGMS-UHFFFAOYSA-N AEBSF hydrochloride Chemical compound Cl.NCCC1=CC=C(S(F)(=O)=O)C=C1 WRDABNWSWOHGMS-UHFFFAOYSA-N 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 102000030523 Catechol oxidase Human genes 0.000 description 1
- 108010031396 Catechol oxidase Proteins 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 101001016865 Homo sapiens Heat shock protein HSP 90-alpha Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- YKTSYUJCYHOUJP-UHFFFAOYSA-N [O--].[Al+3].[Al+3].[O-][Si]([O-])([O-])[O-] Chemical compound [O--].[Al+3].[Al+3].[O-][Si]([O-])([O-])[O-] YKTSYUJCYHOUJP-UHFFFAOYSA-N 0.000 description 1
- 150000001242 acetic acid derivatives Chemical class 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 229910000323 aluminium silicate Inorganic materials 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940072107 ascorbate Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 238000011210 chromatographic step Methods 0.000 description 1
- 150000001860 citric acid derivatives Chemical class 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000009295 crossflow filtration Methods 0.000 description 1
- 238000010908 decantation Methods 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- LMBWSYZSUOEYSN-UHFFFAOYSA-N diethyldithiocarbamic acid Chemical compound CCN(CC)C(S)=S LMBWSYZSUOEYSN-UHFFFAOYSA-N 0.000 description 1
- 239000001177 diphosphate Substances 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- VHJLVAABSRFDPM-ZXZARUISSA-N dithioerythritol Chemical compound SC[C@H](O)[C@H](O)CS VHJLVAABSRFDPM-ZXZARUISSA-N 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 229950004394 ditiocarb Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 231100000463 ecotoxicology Toxicity 0.000 description 1
- 238000004193 electrokinetic chromatography Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000012149 elution buffer Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- DEFVIWRASFVYLL-UHFFFAOYSA-N ethylene glycol bis(2-aminoethyl)tetraacetic acid Chemical compound OC(=O)CN(CC(O)=O)CCOCCOCCN(CC(O)=O)CC(O)=O DEFVIWRASFVYLL-UHFFFAOYSA-N 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000002657 fibrous material Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 102000037865 fusion proteins Human genes 0.000 description 1
- 108020001507 fusion proteins Proteins 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- -1 leupeptide Chemical compound 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 108010091212 pepstatin Proteins 0.000 description 1
- 229950000964 pepstatin Drugs 0.000 description 1
- FAXGPCHRFPCXOO-LXTPJMTPSA-N pepstatin A Chemical compound OC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C[C@H](O)[C@H](CC(C)C)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C(C)C)NC(=O)CC(C)C FAXGPCHRFPCXOO-LXTPJMTPSA-N 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 239000004476 plant protection product Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000004952 protein activity Effects 0.000 description 1
- 230000012846 protein folding Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000012465 retentate Substances 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 206010040560 shock Diseases 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- JVBXVOWTABLYPX-UHFFFAOYSA-L sodium dithionite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])=O JVBXVOWTABLYPX-UHFFFAOYSA-L 0.000 description 1
- CSRCBLMBBOJYEX-UHFFFAOYSA-M sodium;2-morpholin-4-ylethanesulfonic acid;hydroxide Chemical compound [OH-].[Na+].OS(=O)(=O)CCN1CCOCC1 CSRCBLMBBOJYEX-UHFFFAOYSA-M 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Peptides Or Proteins (AREA)
Claims (15)
1. Metode til at genindvinde et stressprotein fra en plante, hvor metoden omfatter at indvinde, fra ovennævnte plante, en væske, der omfatter stressproteinet; at udskille, fra ovennævnte væske, en eller flere komponenter ud over stressproteinet; at tilføje en fibrøs filterhjælp og et mineralsk pulver til væsken; derefter udsætte væsken, der omfatter stressproteinet, samt de(n) udskilte komponent(er), den fibrøse filterhjælp og det mineralske pulver, for et første filtreringstrin over et filter, og derved separere den udskilte, fibrøse filterhjælp og det mineralske pulver fra filtratet, der omfatter stressproteinet; derefter udsætte filtratet for et yderligere filtreringstrin; og derefter rense stressproteinet.
2. Metode, ifølge krav 1, hvor, i det første filtreringstrin, klorofyl og/eller rubisco ijemes fra væsken.
3. Metode, ifølge krav 1 eller 2, hvor det mineralske pulver omfatter mindst ét komet mineral.
4. Metode, ifølge krav 3, der omfatter mindst ét komet mineral, udvalgt fra gruppen diatoméjord og perlit.
5. Metode, ifølge et hvilket som helst af ovenstående krav, hvor den fibrøse filterhjælp omfatter cellulosefibre.
6. Metode, ifølge et hvilket som helst af ovenstående krav, hvor den fibrøse filterhjælp er en blanding af fibre med forskellige længder, og hvor blandingen leverer i det mindste fibre med en længde i et omfang, der strækker sig fra cirka 20 pm til cirka 130 μηι.
7. Metode, ifølge et hvilket som helst af ovenstående krav, hvor det yderligere filtreringstrin, som filtratet er udsat for, er et dybdefiltreringstrin.
8. Metode, ifølge et hvilket som helst af ovenstående krav, hvor, efter det yderligere filtreringstrin, en fraktion, der omfatter stressproteinet, er udsat for et væskefjemelsestrin, hvorved stressproteinet koncentreres i fraktionen, helst ved omvendt osmose, ultrafiltrering eller nanofiltrering, og/eller for et saltijcrnclsestrin, helst ved dialyse.
9. Metode, ifølge et hvilket som helst af ovenstående krav, hvor stressproteinet renses ved kromatografi.
10. Metode, ifølge krav 9, hvor kromatografien omfatter et første stærkt anionbytningstrin, derefter et affinitetskromatografi-adskillelsestrin, og derefter et andet stærkt anionbytningstrin.
11. Metode, ifølge krav 10, hvor den gennemsnitlige partikelstørrelse af bytningsmaterialet, der anvendes i den første anionbytning, er større end den gennemsnitlige partikelstørrelse af bytningsmaterialet, der anvendes i den anden anionbytning.
12. Metode, ifølge et hvilket som helst af ovenstående krav, hvor stressproteinet er et stressprotein fra en plante, udvalgt fra gruppen Fabaceae, Chenopodiaceae, Solanaceae og Poaceae, især fra gruppen lucerne, hvede, majs, ris, soja, kartoffel, bede, mere især fra lucerne.
13. Metode, ifølge et hvilket som helst af ovenstående krav, hvor stressproteinet er udvalgt fra gruppen HSP40, HSP60, HSP70 og HSP90.
14. Metode, ifølge et hvilket som helst af ovenstående krav, hvor filtratet fra det første filtreringstrin er udsat for ovennævnte yderligere filtreringstrin uden først at være blevet udsat for centrifugering.
15. Anvendelse af mindst ét komet mineral, udvalgt fra gruppen diatoméjord og perlit, til at fjerne klorofyl og/eller carotenoid fra en væske, især en plantesaft, der indeholder et varmechokprotein.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09157774 | 2009-04-09 | ||
| PCT/EP2010/054723 WO2010115990A1 (en) | 2009-04-09 | 2010-04-09 | Filtration of a liquid comprising a plant stress protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2417153T3 true DK2417153T3 (da) | 2014-12-01 |
Family
ID=40943897
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10713337.3T DK2417153T3 (da) | 2009-04-09 | 2010-04-09 | Filtrering af en væske, der omfatter et stressprotein fra en plante |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8933200B2 (da) |
| EP (1) | EP2417153B1 (da) |
| DK (1) | DK2417153T3 (da) |
| ES (1) | ES2524541T3 (da) |
| WO (1) | WO2010115990A1 (da) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2928485B1 (en) | 2012-12-07 | 2018-04-04 | Alfa Biogene International B.V. | Hsp for use in treatment for imiquimod related side effects |
| CN109535240B (zh) * | 2018-11-02 | 2022-05-20 | 广东海洋大学 | 一种改善鱼肉肌球蛋白性能的方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3872016A (en) | 1974-03-25 | 1975-03-18 | Johns Manville | Filter aid materials dispersible in organic liquids |
| DE19804882A1 (de) | 1998-02-09 | 1999-08-12 | Herzog Stefan | Filterhilfsmittel |
| GEP20002333B (en) | 1999-05-19 | 2000-09-10 | Method for Inducing Synthesis of Heat Stress Proteins in Herbal Plants | |
| NL1018211C2 (nl) | 2001-06-05 | 2002-12-10 | Alfa Biogene Internat B V | Het winnen van een stress-eiwit. |
| ATE493432T1 (de) | 2003-11-12 | 2011-01-15 | Alfa Biogene Internat B V | Gewinnung von hitzeschockproteinen |
| CA2615372A1 (en) | 2007-07-13 | 2009-01-13 | Marc-Andre D'aoust | Influenza virus-like particles (vlps) comprising hemagglutinin |
-
2010
- 2010-04-09 DK DK10713337.3T patent/DK2417153T3/da active
- 2010-04-09 ES ES10713337.3T patent/ES2524541T3/es active Active
- 2010-04-09 EP EP10713337.3A patent/EP2417153B1/en not_active Not-in-force
- 2010-04-09 WO PCT/EP2010/054723 patent/WO2010115990A1/en not_active Ceased
- 2010-04-09 US US13/263,445 patent/US8933200B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20120095189A1 (en) | 2012-04-19 |
| WO2010115990A1 (en) | 2010-10-14 |
| ES2524541T3 (es) | 2014-12-10 |
| EP2417153A1 (en) | 2012-02-15 |
| EP2417153B1 (en) | 2014-11-05 |
| US8933200B2 (en) | 2015-01-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2460539C2 (ru) | СПОСОБ ОЧИСТКИ ПОЛИСАХАРИДОВ Streptococcus pneumoniae 3 ТИПА (ВАРИАНТЫ) | |
| AU2018231399B2 (en) | Method for purifying proteins using silicate | |
| WO2006095964A1 (en) | Method for abstract of liquid extract from chlorella | |
| DK2417153T3 (da) | Filtrering af en væske, der omfatter et stressprotein fra en plante | |
| CA3092460C (en) | ANALYTE SEPARATION POLYMER AND METHODS FOR PREPARING AND USING THIS POLYMER | |
| JPS60149526A (ja) | 溶血血液の未変性形の主タンパクの調製方法 | |
| EP1531160B1 (en) | Recovery of a heat shock protein | |
| KR102245547B1 (ko) | 고밀도의 미정제 세포 배양 수거물의 정화를 위한 방법 | |
| IL278584B2 (en) | Method and system of protein extraction | |
| CN119529313B (zh) | 一种皂苷佐剂纯化方法 | |
| TW202012433A (zh) | 一種重組人胰島素或其類似物的前驅物的製備方法 | |
| WO2003057720A3 (en) | Recovery of biofilament proteins from biological fluids | |
| US5407579A (en) | Hemoglobin purification | |
| JPH06145186A (ja) | α，α−トレハロースの製造法 | |
| JPS58500737A (ja) | 植物の葉から蛋白質の単離法 | |
| US20040260066A1 (en) | Method for purifying mixtures of immunoglobulin & albumin | |
| CN107827965B (zh) | 一种面包树果实凝集素的提取方法 | |
| CN110616213A (zh) | 一种碱性金属蛋白酶的分离纯化方法 | |
| RU2467783C2 (ru) | Способ хроматографического выделения иммуноглобулина | |
| JP5931363B2 (ja) | タンパク質の精製方法 | |
| JP2020125243A (ja) | セレノネインモノマーの分離方法 | |
| FI56844C (fi) | Foerfarande foer separering av utfaellda plasmaproteiner ur albuminhaltiga suspensioner | |
| WO2006137815A2 (en) | Method for purifying mixtures of immunoglobulin & albumin | |
| CN121248763A (zh) | 一种基于疏水层析的重组胶原蛋白纯化工艺 | |
| WO2024077117A1 (en) | Method for making vesicles |