DK2358866T3 - Corynebakteriestamme med forstærket 5'-xanthosinmonophosphat-produktivitet og fremgangsmåde til fremstilling af 5'-xanthosinmonophosphat under anvendelse af denne - Google Patents

Corynebakteriestamme med forstærket 5'-xanthosinmonophosphat-produktivitet og fremgangsmåde til fremstilling af 5'-xanthosinmonophosphat under anvendelse af denne Download PDF

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DK2358866T3
DK2358866T3 DK09833645.6T DK09833645T DK2358866T3 DK 2358866 T3 DK2358866 T3 DK 2358866T3 DK 09833645 T DK09833645 T DK 09833645T DK 2358866 T3 DK2358866 T3 DK 2358866T3
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gene
mqo
strain
malate dehydrogenase
xmp
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DK09833645.6T
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Jinman Cho
Hye Won Kim
Jinnam Lee
Ji-Hye Lee
Yoon Seok Oh
Jang Hee Park
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Cj Cheiljedang Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (8)

1. Anvendelse af en corynebakteriestamme til fremstilling af xanthosin-5'-monophosphat, hvor corynebakteriestammen har en malatdehydrogenase-aktivitet, der er højere end sin endogene vildtypeaktivitet, ved at øge antallet af mqo-geneksemplarer, der koder malatdehydrogenasen, eller ved at erstatte eller modificere en promotor af et mqo-gen.
2. Anvendelse ifølge krav 1, hvor malatdehydrogenaseaktiviteten er forbedret som et resultat af en øgning i mqo-genekspression.
3. Anvendelse ifølge krav 2, hvor mqo-genet har SEQ ID NO: 7.
4. Anvendelse ifølge krav 1, hvor stammen er transformeret med en rekom-binant vektor omfattende et mqo-gen.
5. Anvendelse ifølge krav 4, hvor mqo-genet har SEQ ID NO: 7.
6. Anvendelse ifølge krav 1, hvor corynebakteriestammen er corynebakterie-ammoniakgener.
7. Fremgangsmåde til fremstilling af xanthosin-5'-monophosphat, omfattende at dyrke en corynebakteriestamme med en malatdehydrogenaseaktivitet, der er højere end dens endogene vildtypeaktivitet, ved at øge antallet af mqo-geneksemplarer, der koder malatdehydrogenasen, eller ved at erstatte eller modificere en promotor af et mqo-gen; og at opnå xanthosin-5'-monophosphat ud fra kulturen.
8. Corynebakterie-ammoniakgenstamme med øget xanthosin-5'-monophos-phat-produktivitet, hvilken har en malatdehydrogenaseaktivitet, der er højere end dens endogene vildtypeaktivitet, ved at øge antallet af mqo-geneksemplarer, der koder malatdehydrogenasen, eller ved at erstatte eller modificere en promotor af et mqo-gen.
DK09833645.6T 2008-12-17 2009-12-17 Corynebakteriestamme med forstærket 5'-xanthosinmonophosphat-produktivitet og fremgangsmåde til fremstilling af 5'-xanthosinmonophosphat under anvendelse af denne DK2358866T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020080128847A KR101072708B1 (ko) 2008-12-17 2008-12-17 ５＇－크산틸산 생산능이 향상된 미생물 및 이를 이용한 ５＇－크산틸산의 생산방법
PCT/KR2009/007559 WO2010071367A2 (en) 2008-12-17 2009-12-17 A corynebacteria strain having enhanced 5'-xanthosine monophosphate productivity and a method of producing 5'-xanthosine monophosphate using the same

Publications (1)

Publication Number Publication Date
DK2358866T3 true DK2358866T3 (da) 2015-11-23

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Application Number Title Priority Date Filing Date
DK09833645.6T DK2358866T3 (da) 2008-12-17 2009-12-17 Corynebakteriestamme med forstærket 5'-xanthosinmonophosphat-produktivitet og fremgangsmåde til fremstilling af 5'-xanthosinmonophosphat under anvendelse af denne

Country Status (8)

Country Link
US (1) US8815545B2 (da)
EP (1) EP2358866B1 (da)
JP (1) JP5553842B2 (da)
KR (1) KR101072708B1 (da)
CN (2) CN104130966A (da)
DK (1) DK2358866T3 (da)
ES (1) ES2553571T3 (da)
WO (1) WO2010071367A2 (da)

Families Citing this family (5)

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Publication number Priority date Publication date Assignee Title
KR101072720B1 (ko) * 2008-12-17 2011-10-11 씨제이제일제당 (주) ５＇－구아닐산 생산능이 향상된 미생물 및 이를 이용한 ５＇－구아닐산의 생산방법
KR101929158B1 (ko) * 2018-06-07 2018-12-13 씨제이제일제당 (주) 5'-크산틸산을 생산하는 미생물 및 이를 이용한 5'-크산틸산의 제조방법
KR102273637B1 (ko) * 2021-01-29 2021-07-06 씨제이제일제당 주식회사 신규한 펩티딜-디펩티다제 변이체 및 이를 이용한 xmp 또는 gmp 생산 방법
KR102288398B1 (ko) * 2021-01-29 2021-08-10 씨제이제일제당 주식회사 신규한 nad(p)-의존성 산화환원효소 변이체 및 이를 이용한 xmp 또는 gmp 생산 방법
KR102258159B1 (ko) * 2021-01-29 2021-05-27 씨제이제일제당 (주) 신규한 말레이트 디하이드로게나제 변이체 및 이를 이용한 l-라이신 생산 방법

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US20020028490A1 (en) 1999-03-19 2002-03-07 Douwe Molenaar Process for the production of L-amino acids by fermentation using coryneform bacteria
DE19912384A1 (de) * 1999-03-19 2000-09-21 Degussa Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien
AU2001265835A1 (en) * 2000-06-23 2002-01-08 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
DE10032350A1 (de) * 2000-07-04 2002-01-24 Degussa Neue für das mdhA-Gen kodierende Nukleotidsequenzen
KR100405990B1 (ko) 2000-10-04 2003-11-20 김성준 유기게르마늄 함유 동충하초의 제조방법
RU2209249C2 (ru) 2000-11-22 2003-07-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Способ получения ксантозин-5'-монофосфата, штамм corynebacterium ammoniagenes - продуцент ксантозин-5'-монофосфата (варианты)
KR100402320B1 (ko) 2001-01-05 2003-10-22 씨제이 주식회사 5'-크산틸산을 고수율로 생산하는 미생물 코리네박테리움암모니아게네스 엔브이4-82-9 및 그를 이용한 5'-크산틸산생산방법
DE10117816A1 (de) * 2001-04-10 2002-10-17 Degussa Verfahren zur fermentativen Herstellung von L-Aminosäuren unter Verwendung coryneformer Bakterien
KR100505925B1 (ko) * 2002-12-11 2005-08-03 씨제이 주식회사 5’-크산틸산을 생산하는 미생물
KR20040051731A (ko) * 2002-12-11 2004-06-19 씨제이 주식회사 5’-크산틸산을 생산하는 미생물
KR100542568B1 (ko) * 2003-12-10 2006-01-11 씨제이 주식회사 5'-크산틸산을 생산하는 미생물
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KR100789274B1 (ko) * 2007-01-15 2008-01-02 씨제이 주식회사 코리네박테리움 글루타미쿰에서 유래한 신규한 프로모터핵산 분자, 그 프로모터를 포함하는 재조합 벡터, 그재조합 벡터를 포함하는 숙주 세포 및 그 숙주 세포를이용하여 유전자를 발현하는 방법
KR100957690B1 (ko) 2008-01-22 2010-05-12 씨제이제일제당 (주) 5'-크산틸산 생산능이 향상된 코리네박테리움 속 미생물및 이를 이용한 5'-크산틸산의 생산 방법
KR101072720B1 (ko) 2008-12-17 2011-10-11 씨제이제일제당 (주) ５＇－구아닐산 생산능이 향상된 미생물 및 이를 이용한 ５＇－구아닐산의 생산방법
KR101210704B1 (ko) * 2010-03-19 2012-12-10 씨제이제일제당 (주) ５'－크산틸산 및 ５'－구아닐산 생산능이 향상된 미생물 및 이를 이용한 5'－크산틸산 또는 ５'－구아닐산의 생산방법

Also Published As

Publication number Publication date
CN102317433A (zh) 2012-01-11
WO2010071367A3 (en) 2010-11-04
KR20100070219A (ko) 2010-06-25
JP2012511925A (ja) 2012-05-31
WO2010071367A2 (en) 2010-06-24
EP2358866A4 (en) 2012-08-22
CN104130966A (zh) 2014-11-05
US20110300582A1 (en) 2011-12-08
ES2553571T3 (es) 2015-12-10
EP2358866A2 (en) 2011-08-24
JP5553842B2 (ja) 2014-07-16
EP2358866B1 (en) 2015-10-07
CN102317433B (zh) 2016-09-07
KR101072708B1 (ko) 2011-10-11
US8815545B2 (en) 2014-08-26

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