DK2322630T3 - Polypeptider med xylanaseaktivitet og polynukleotider, der koder for dem - Google Patents

Polypeptider med xylanaseaktivitet og polynukleotider, der koder for dem Download PDF

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Publication number
DK2322630T3
DK2322630T3 DK11152474.0T DK11152474T DK2322630T3 DK 2322630 T3 DK2322630 T3 DK 2322630T3 DK 11152474 T DK11152474 T DK 11152474T DK 2322630 T3 DK2322630 T3 DK 2322630T3
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Denmark
Prior art keywords
polypeptide
ala
sequence
seq
aspergillus
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DK11152474.0T
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English (en)
Inventor
Debbie Yaver
Paul Harris
Suzanne Otani
Janine Lin
Ge Haiyan
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Novozymes Inc
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Publication of DK2322630T3 publication Critical patent/DK2322630T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)

Claims (12)

1. Isoleret polypeptid med xylanaseaktivitet, som er valgt fra gruppen bestående af: et polypeptid med en aminosyresekvens, der udviser mindst 95% identitet med aminosyrerne 20 til 397 ifølge SEQ ID NO: 6.
2. Polypeptid ifølge krav 1, som omfatter aminosyresekvensen ifølge SEQ ID NO: 6.
3. Polypeptid ifølge krav 1, som består af SEQ ID NO: 6 eller et fragment deraf, som har xylanaseaktivitet og har mindst 360 aminosyrerester.
4. Polypeptid ifølge krav 3, som består af SEQ ID NO: 6.
5. Polypeptid ifølge krav 3, som består af aminosyrerne 20 til 397 ifølge SEQ ID NO: 6.
6. Polypeptid ifølge krav 1, som kodes for af polynukleotidet indeholdt i plasmid pHyGe009, hvilket plasmid er indeholdt i E. coli NRRL B-30703.
7. Isoleret polynukleotid, som omfatter en nukleotidsekvens, der koder for polypeptidet ifølge et hvilket som helst af kravene 1-6.
8. Nukleinsyrekonstruktion, som omfatter polynukleotidet ifølge krav 7 operativt forbundet til en eller flere kontrolsekvenser, der styrer fremstillingen af polypeptidet i en ekspressionsvært.
9. Rekombinant ekspressionsvektor, som omfatter nukleinsyrekonstruktionen ifølge krav 8.
10. Rekombinant værtscelle, som omfatter den rekombinante ekspressionsvektor ifølge krav 9.
11. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-6, hvilken fremgangsmåde omfatter: (a) dyrkning af en celle, som i sin vildtypeform kan fremstille polypeptidet, under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
12. Fremgangsmåde til fremstilling af polypeptidet ifølge et hvilket som helst af kravene 1-6, hvilken fremgangsmåde omfatter: (a) dyrkning af en værtscelle, som omfatter en nukleinsyrekonstruktion, hvilken nukleinsyrekonstruktion omfatter en nukleotidsekvens, der koder for polypeptidet, under betingelser, som er befordrende for fremstilling af polypeptidet; og (b) indvinding af polypeptidet.
DK11152474.0T 2004-02-12 2005-02-09 Polypeptider med xylanaseaktivitet og polynukleotider, der koder for dem DK2322630T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US54443104P 2004-02-12 2004-02-12
US54446104P 2004-02-12 2004-02-12
US54442904P 2004-02-12 2004-02-12
EP05856580A EP1715736A4 (en) 2004-02-12 2005-02-09 POLYPEPTIDES HAVING XYLANASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME

Publications (1)

Publication Number Publication Date
DK2322630T3 true DK2322630T3 (da) 2017-02-13

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DK11152474.0T DK2322630T3 (da) 2004-02-12 2005-02-09 Polypeptider med xylanaseaktivitet og polynukleotider, der koder for dem

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US (5) US7960160B2 (da)
EP (2) EP1715736A4 (da)
CN (1) CN101389645B (da)
DK (1) DK2322630T3 (da)
WO (1) WO2006078256A2 (da)

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