DK2313514T3 - Degradering af lignocellulosemateriale - Google Patents

Degradering af lignocellulosemateriale Download PDF

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Publication number
DK2313514T3
DK2313514T3 DK09781475.0T DK09781475T DK2313514T3 DK 2313514 T3 DK2313514 T3 DK 2313514T3 DK 09781475 T DK09781475 T DK 09781475T DK 2313514 T3 DK2313514 T3 DK 2313514T3
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Denmark
Prior art keywords
enzyme
acid
composition
glucose
sugars
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DK09781475.0T
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English (en)
Inventor
De Wilhelmus Theodorus Antonius Maria Laat
Manoj Kumar
Margot Elisabeth Francoise Schooneveld-Bergmans
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Dsm Ip Assets Bv
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C13—SUGAR INDUSTRY
    • C13K—SACCHARIDES OBTAINED FROM NATURAL SOURCES OR BY HYDROLYSIS OF NATURALLY OCCURRING DISACCHARIDES, OLIGOSACCHARIDES OR POLYSACCHARIDES
    • C13K1/00—Glucose; Glucose-containing syrups
    • C13K1/02—Glucose; Glucose-containing syrups obtained by saccharification of cellulosic materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (15)

1. Fremgangsmåde til behandling af majsblade og -stilke eller hvedehalm, hvilken fremgangsmåde omfatter etablering af kontakt mellem forbehandlet lignocellulosemateriale udvalgt fra gruppen, der består af majsblade og -stilke eller hvedehalm, og en sammensætning, der omfatter tre eller flere enzymaktiviteter, hvilke enzymaktiviteter er cellulaseaktiviteter og eventuelt også hemicellulaseaktiviteter, hvor sammensætningen omfatter en endoglucanase, cellobiohydrolase og β-glucosidase, som alle stammer fra Talaromyces emersonii, og hvor pH under behandlingen er 4,5 eller derunder, og behandlingen udføres ved et tørstofindhold på 15 % eller derover.
2. Fremgangsmåde ifølge krav 1, hvor pH under behandlingen er 4,0 eller derunder.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor enzymaktiviteterne har et temperaturoptimum på 50 °C eller derover.
4. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor sammensætningen omfatter endo-1,3 (1,4)-β-glucanaseaktivitet og endo-β-Ι,4-glucanaseaktivitet, hvor begge aktiviteter stammer fra Talaromyces emersonii.
5. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor sammensætningen omfatter en eller flere xylanaseaktiviteter.
6. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor sammensætningen omfatter et expansin, et expansinlignende protein, et celluloseinduceret protein, et celluloseintegrerende protein, et scaffoldin eller et scaffoldinlignende protein, der eventuelt stammer fra Talaromyces emersonii.
7. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor behandlingen omfatter hydrolyse og/eller modificering af cellulose og/eller hemicellulose.
8. Fremgangsmåde til fremstilling af et sukker eller flere sukre fra majsblade og -stilke eller hvedehalm, hvilken fremgangsmåde omfatter etablering af kontakt mellem forbehandlet lignocellulosemateriale udvalgt fra gruppen, der består af majsblade og -stilke eller hvedehalm, og en sammensætning ifølge et hvilket som helst af kravene 1 til 7.
9. Fremgangsmåde ifølge krav 8, hvor sukrene er monomere og/eller multimere sukre.
10. Fremgangsmåde ifølge krav 9, hvor mindst ét af de dannede sukre er et fermenterbart sukker.
11. Fremgangsmåde ifølge krav 10, hvor mindst ét af de dannede sukre er glucose, cellobiose, xylose, arabinose, galactose, galacturonsyre, glucuronsyre, mannose, rhamnose, sucrose eller fructose.
12. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor forbehandlingen omfatter eksponering af majsbladene og -stilkene eller hvedehalmen for en syre, en base, et opløsningsmiddel, varme, et peroxid, ozon, mekanisk fragmentering, formaling, knusning eller hurtig trykreduktion eller en kombination af hvilke som helst to eller flere deraf.
13. Fremgangsmåde til fremstilling af et fermenteringsprodukt, hvilken fremgangsmåde omfatter: - fremstilling af et fermenterbart sukker ved anvendelse af en fremgangsmåde ifølge et hvilket som helst af ovennævnte krav; og - fermentering af det resulterende fermenterbare sukker til derved fremstilling af et fermenteringsprodukt.
14. Fremgangsmåde ifølge krav 13, hvor fermenteringsproduktet er ethanol, butanol, mælkesyre, en plastik, en organisk syre, et opløsningsmiddel, et dyrefodertilskud, et farmaceutikum, et vitamin, en aminosyre, et enzym eller et kemisk råstof.
15. Fremgangsmåde ifølge krav 13 eller 14, hvor fermenteringen udføres i SHF-tilstand, og glucosekoncentrationen er 50-200 g/1.
DK09781475.0T 2008-08-11 2009-08-04 Degradering af lignocellulosemateriale DK2313514T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08162154 2008-08-11
PCT/EP2009/060098 WO2010018105A1 (en) 2008-08-11 2009-08-04 Degradation of lignocellulosic material

Publications (1)

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DK2313514T3 true DK2313514T3 (da) 2017-02-20

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Country Status (10)

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US (2) US10557153B2 (da)
EP (2) EP2313514B1 (da)
CN (2) CN106978455A (da)
AU (1) AU2009281253B2 (da)
BR (1) BRPI0917996A2 (da)
CA (1) CA2731983C (da)
DK (1) DK2313514T3 (da)
EA (1) EA020808B1 (da)
MX (1) MX2011001535A (da)
WO (1) WO2010018105A1 (da)

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Publication number Publication date
CN106978455A (zh) 2017-07-25
US20110143402A1 (en) 2011-06-16
EA201100346A1 (ru) 2011-08-30
CA2731983C (en) 2017-02-14
MX2011001535A (es) 2011-03-28
EP2313514B1 (en) 2016-11-16
EP3156495A1 (en) 2017-04-19
US10557153B2 (en) 2020-02-11
AU2009281253B2 (en) 2014-11-27
EA020808B1 (ru) 2015-01-30
WO2010018105A1 (en) 2010-02-18
US20200123577A1 (en) 2020-04-23
EP2313514A1 (en) 2011-04-27
BRPI0917996A2 (pt) 2015-06-16
AU2009281253A1 (en) 2010-02-18
CN102119219A (zh) 2011-07-06
CA2731983A1 (en) 2010-02-18

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