DK2239336T3 - Mikroorganisme til fremstilling af L-aminosyrer og fremgangsmåde til fremstilling af L-aminosyrer under anvendelse af samme - Google Patents

Mikroorganisme til fremstilling af L-aminosyrer og fremgangsmåde til fremstilling af L-aminosyrer under anvendelse af samme Download PDF

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DK2239336T3
DK2239336T3 DK10715651.5T DK10715651T DK2239336T3 DK 2239336 T3 DK2239336 T3 DK 2239336T3 DK 10715651 T DK10715651 T DK 10715651T DK 2239336 T3 DK2239336 T3 DK 2239336T3
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sucrose
microorganism
amino acid
gene
mannokinase
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DK10715651.5T
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English (en)
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Jae Yeong Ju
Kwang Ho Lee
Hyun Ae Bae
Hyo Jin Kim
Keun Chul Lee
Young Bin Hwang
Kyu Soo Cho
Hye Min Park
Hyun Ah Kim
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Cj Cheiljedang Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/2488—Mannanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01007—Mannokinase (2.7.1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01048—Sucrose alpha-glucosidase (3.2.1.48), i.e. sucrase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
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  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (15)

1. Mikroorganisme, som tilhørerslægten Escherichia, med forbedret L-aminosyre-produktivitet og saccharose-assimilerende evne, og som har aktiviteterne af saccha-rosepermease, saccharosehydrolase og saccharosetranskriptionsregulator og har en forøget mannokinaseaktivitet i sammenligning med en saccharose-ikke-assimilerende mikroorganisme, som tilhører slægten Escherichia.
2. Mikroorganisme ifølge krav 1, hvor den forøgede mannokinaseaktivitet opnås ved én eller flere fremgangsmåder, som er valgt fra gruppen bestående af en forøgelse af kopitallet af mannokinasegenet, promotorudskiftning, promotormutation og genmutation.
3. Mikroorganisme ifølge krav 2, hvor forøgelsen af kopitallet af mannokinasegenet opnås ved at indsætte et polynukleotid, som koder for mannokinase, i et kromosom eller indføre polynukleotidet i et vektorsystem.
4. Mikroorganisme ifølge krav 3, hvor vektoren indeholderen basesekvens som i SEQ ID NO:15.
5. Mikroorganisme ifølge krav 1, hvor aktiviteterne af saccharosepermease, saccharosehydrolase og saccharosetranskriptionsregulator bibringes ved transformation med en vektor, som indeholder basesekvenser, der koder for saccharosepermease, saccharosehydrolase og saccharosetranskriptionsregulator.
6. Mikroorganisme ifølge krav 5, hvor basesekvenserne er basesekvenser som i SEQ ID NO:4, 6 og 7.
7. Mikroorganisme ifølge krav 5, hvor saccharosetranskriptionsregulatoren kodes afen basesekvens som i SEQ ID NO:17.
8. Mikroorganisme ifølge krav 1, hvor en vektor, som indeholder alle basesekvenser, der koder for saccharosepermease, saccharosehydrolase, saccharosetranskriptions-regulator og mannokinase, anvendes til at udføre transformation.
9. Mikroorganisme ifølge krav 8, hvor vektoren kodes af en basesekvens som i SEQ ID NO:18.
10. Mikroorganisme ifølge krav 1, hvor den mikroorganisme, der tilhører slægten Escherichia, er E. col i.
11. Mikroorganisme ifølge krav 10, hvor E. coli er E. coli CA03-0308 med deponeringsnummer: KCCM10978P.
12. Mikroorganisme ifølge krav 1, hvor L-aminosyren er valgt fra gruppen bestående af L-threonin, O-succinyl-homoserin, O-acetyl-homoserin, L-methionin, L-lysin, L-homo-serin, L-isoleucin, L-valin og L-tryptophan.
13. Mikroorganisme ifølge krav 12, hvor L-aminosyren er valgt fra gruppen bestående af L-threonin, O-succinyl-homoserin, O-acetyl-homoserin og L-tryptophan.
14. Fremgangsmåde til fremstilling af en L-aminosyre, hvilken fremgangsmåde omfatter følgende trin: inokulering og dyrkning af en £sc/7en'c/7/'a-mikroorganisme ifølge et hvilket som helst af kravene 1 til 13, som har forbedret L-aminosyreproduktivitet og saccharose-assimilerende evne, i et dyrkningsmedium, som helt eller delvist indeholder saccharose som carbonkilde; og separering af L-aminosyren fra det i ovenstående trin opnåede dyrkningsmedium.
15. Fremgangsmåde ifølge krav 14, hvor L-aminosyren er valgt fra gruppen bestående af L-threonin, O-succinyl-homoserin, O-acetyl-homoserin, L-methionin, L-lysin, L-homo-serin, L-isoleucin, L-valin og L-tryptophan.
DK10715651.5T 2009-02-13 2010-02-11 Mikroorganisme til fremstilling af L-aminosyrer og fremgangsmåde til fremstilling af L-aminosyrer under anvendelse af samme DK2239336T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090012048A KR101083136B1 (ko) 2009-02-13 2009-02-13 Ｌ-아미노산 생산용 미생물 및 이를 이용하여 ｌ-아미노산을 생산하는 방법
PCT/KR2010/000872 WO2010093182A2 (ko) 2009-02-13 2010-02-11 Ｌ-아미노산 생산용 미생물 및 이를 이용하여 ｌ-아미노산을 생산하는 방법

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DK2239336T3 true DK2239336T3 (da) 2017-08-14

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US (1) US8574874B2 (da)
EP (1) EP2239336B1 (da)
JP (1) JP5597554B2 (da)
KR (1) KR101083136B1 (da)
CN (1) CN102165056B (da)
DK (1) DK2239336T3 (da)
ES (1) ES2635312T3 (da)
HU (1) HUE032951T2 (da)
PL (1) PL2239336T3 (da)
WO (1) WO2010093182A2 (da)

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KR101145943B1 (ko) * 2010-03-11 2012-05-15 씨제이제일제당 (주) Ｌ-아미노산 생산능을 갖는 미생물 및 이를 이용하여 ｌ-아미노산을 생산하는 방법
US8222000B2 (en) 2010-12-06 2012-07-17 E I Du Pont De Nemours And Company Recombinant bacteria having the ability to metabolize sucrose
US8129170B1 (en) 2010-12-06 2012-03-06 E.I. Du Pont De Nemours And Company Recombinant bacteria having the ability to metabolize sucrose
KR101261147B1 (ko) 2011-01-18 2013-05-06 씨제이제일제당 (주) Ｌ-아미노산의 생산능이 향상된 미생물 및 이를 이용하여 ｌ-아미노산을 생산하는 방법
KR101294935B1 (ko) 2011-04-01 2013-08-08 씨제이제일제당 (주) 에세리키아 속 균주에서 유래된 프락토키나제 유전자가 도입된 코리네박테리움 속 균주 및 상기 균주를 이용하여 ｌ-아미노산을 생산하는 방법
PL2803722T3 (pl) * 2012-01-10 2018-03-30 Cj Cheiljedang Corporation Mikroorganizmy maczugowców, które mogą wykorzystywać ksylozę i sposób wytwarzania L-lizyny z ich wykorzystaniem
US8686114B2 (en) 2012-03-05 2014-04-01 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides
KR20140102393A (ko) * 2013-02-13 2014-08-22 씨제이제일제당 (주) L-쓰레오닌 생산능을 가지는 재조합 에스케리키아 속 미생물 및 이를 이용한 l-쓰레오닌의 생산방법
MY171291A (en) 2013-04-16 2019-10-07 Cj Cheiljedang Corp Microorganisms having l-tryptophan productivity and a method for production of l-tryptophan using same
PL2927316T3 (pl) * 2014-03-31 2019-05-31 Jennewein Biotechnologie Gmbh Całkowita fermentacja oligosacharydów
EP3161143A4 (en) 2014-06-27 2018-02-21 Glycom A/S Oligosaccharide production
US11926858B2 (en) 2014-06-27 2024-03-12 Glycom A/S Oligosaccharide production
EP4179102A1 (en) 2020-07-13 2023-05-17 Glycom A/S Oligosaccharide production

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GB2155935B (en) 1984-02-16 1988-10-12 Antibioticos Sa Novel e. coli hybrid plasmid vector conferring sucrose fermenting capacity
RU2212447C2 (ru) * 2000-04-26 2003-09-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Штамм escherichia coli - продуцент аминокислоты (варианты) и способ получения аминокислот (варианты)
US7524660B2 (en) * 2005-05-05 2009-04-28 E.I. Du Pont De Nemours And Company Utilization of fructose in microbial production strains
WO2007037503A1 (en) 2005-09-27 2007-04-05 Ajinomoto Co., Inc. An l-amino acid-producing bacterium and a method for producing an l-amino acid
US9005952B2 (en) 2008-04-04 2015-04-14 Cj Cheiljedang Corporation Microorganism producing L-methionine precursor and the method of producing L-methionine precursor using the microorganism
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KR20100092765A (ko) 2010-08-23
WO2010093182A2 (ko) 2010-08-19
EP2239336A4 (en) 2013-08-21
JP5597554B2 (ja) 2014-10-01
US8574874B2 (en) 2013-11-05
WO2010093182A3 (ko) 2010-12-09
JP2011512867A (ja) 2011-04-28
CN102165056A (zh) 2011-08-24
EP2239336B1 (en) 2017-05-03
EP2239336A2 (en) 2010-10-13
KR101083136B1 (ko) 2011-11-11
CN102165056B (zh) 2014-12-17
ES2635312T3 (es) 2017-10-03
US20110111466A1 (en) 2011-05-12
PL2239336T3 (pl) 2017-09-29
HUE032951T2 (en) 2017-11-28

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