DK2231861T3 - Transgene planter modificeret for mindsket cadmiumtransport, afledte produkter og beslægtede fremgangsmåder. - Google Patents

Transgene planter modificeret for mindsket cadmiumtransport, afledte produkter og beslægtede fremgangsmåder. Download PDF

Info

Publication number
DK2231861T3
DK2231861T3 DK08860815.3T DK08860815T DK2231861T3 DK 2231861 T3 DK2231861 T3 DK 2231861T3 DK 08860815 T DK08860815 T DK 08860815T DK 2231861 T3 DK2231861 T3 DK 2231861T3
Authority
DK
Denmark
Prior art keywords
seq
sequence
nthma
ser
fragment
Prior art date
Application number
DK08860815.3T
Other languages
English (en)
Inventor
Alec Hayes
Chengalrayan Kudithipudi
Der Hoeven Rutger Van
Original Assignee
Philip Morris Products Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40481764&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK2231861(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Philip Morris Products Sa filed Critical Philip Morris Products Sa
Application granted granted Critical
Publication of DK2231861T3 publication Critical patent/DK2231861T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/10—Chemical features of tobacco products or tobacco substitutes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/16—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nutrition Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Claims (14)

1
1. NtHMA-RNAi-konstrukt i stand til inhibering af ekspressionen af et NtHMA-messenger-RNA, til hvilket det svarer, hvilket konstrukt omfatter eller består af: en første sekvens, der har mindst 70% sekvensidentitet med en del af SEQ ID NO:3 eller 47; en anden sekvens og en tredje sekvens, der har en omvendt komplementær sekvens af den første sekvens, anbragt i samme orientering som den første sekvens, hvor den anden sekvens er anbragt mellem den første sekvens og den tredje sekvens, og den anden sekvens er operativt koblet til den første sekvens og til den tredje sekvens.
2. NtHMA-RNAi-konstrukt ifølge krav 1, hvor: (a) den første sekvens har mindst 95% sekvensidentitet med en sekvens valgt blandt én eller flere af gruppen bestående af: exon 1 som defineret i SEQ ID NO:5, et fragment af exon 1 som defineret i SEQ ID NO:5, exon 2 som defineret i SEQ ID NO:7, et fragment af exon 2 som defineret i SEQ ID NO:7, exon 3 som defineret i SEQ ID NO:9, et fragment af exon 3 som defineret i SEQ ID NO:9, exon 4 som defineret i SEQ ID NO: 11, et fragment af exon 4 som defineret i SEQ ID NO: 11, exon 5 som defineret i SEQ ID NO: 13, et fragment af exon 5 som defineret i SEQ ID NO: 13, exon 6 som defineret i SEQ ID NO: 15, et fragment af exon 6 som defineret i SEQ ID NO: 15, exon 7 som defineret i SEQ ID NO: 17, et fragment af exon 7 som defineret i SEQ ID NO: 17, exon 8 som defineret i SEQ ID NO: 19, et fragment af exon 8 som defineret i SEQ ID NO: 19, exon 9 som defineret i SEQ ID NO:21, et fragment af exon 9 som defineret i SEQ ID NO:21, exon 10 som defineret i SEQ ID NO:23, et fragment af exon 10 som defineret i SEQ ID NO:23, exon 11 som defineret i SEQ ID NO:25 og et fragment af exon 11 som defineret i SEQ ID NO:25; (b) den anden sekvens har mindst 95% sekvensidentitet med en sekvens valgt blandt én eller flere af gruppen bestående af: intron 1 som defineret i SEQ ID NO:4, et fragment af intron 1 som defineret i SEQ ID NO:4, intron 2 som defineret i SEQ ID NO:6, et fragment af intron 2 som defineret i SEQ ID NO:6, intron 3 som defineret i SEQ ID NO:8, et fragment af intron 3 som defineret i SEQ ID NO:8, intron 4 som defineret i SEQ ID NO: 10, et fragment af intron 4 som defineret i SEQ ID NO: 10, intron 5 som defineret i SEQ ID NO: 12, et fragment af intron 5 som defineret i SEQ ID NO: 12, intron 6 som defineret i SEQ ID NO: 14, et fragment af intron 6 som defineret i SEQ ID NO: 14, intron 7 som defineret i SEQ ID NO: 16, et fragment af intron 7 som defineret i SEQ ID NO: 16, intron 8 som defineret i SEQ ID NO: 18, et fragment af intron 8 som defineret i SEQ ID NO: 18, intron 9 som defineret i SEQ ID NO:20, et fragment af intron 9 som defineret i SEQ ID NO:20, intron 10 som defineret i SEQ ID NO:22, et fragment af intron 10 som defineret i SEQ ID NO:22, intron 11 som defineret i SEQ ID NO:24, et fragment af intron 11 som defineret i SEQ ID NO: 24, intron 12 som defineret i SEQ ID NO: 26 og et fragment af intron 12 som defineret i SEQ ID NO:26; (c) den tredje sekvens har mindst 95% sekvensidentitet med en sekvens valgt blandt én eller flere af gruppen bestående af: SEQ ID NO:27, et fragment af SEQ ID NO:27, SEQ 2 ID NO:28, et fragment af SEQ ID NO:28, SEQ ID NO:29, et fragment af SEQ ID NO:29, SEQ ID NO:30, et fragment af SEQ ID NO:30, SEQ ID N0:31, et fragment af SEQ ID N0:31, SEQ ID NO:32, et fragment af SEQ ID NO:32, SEQ ID NO:33, et fragment af SEQ ID NO:33, SEQ ID NO:34, et fragment af SEQ ID NO:34, SEQ ID NO:35, et fragment af SEQ ID NO:35, SEQ ID NO:36, et fragment af SEQ ID NO:36, SEQ ID NO:37 og et fragment af SEQ ID NO:37; (d) den første sekvens omfatter SEQ ID NO:38, den anden sekvens omfatter SEQ ID NO:39 og den tredje sekvens omfatter SEQ ID NO:40; (e) den første sekvens omfatter SEQ ID NO:42, den anden sekvens omfatter SEQ ID NO:43 og den tredje sekvens omfatter SEQ ID NO:44 eller (f) én eller to eller tre af (a), (b), (c), (d) og (e).
3. NtHMA-RNAi-konstrukt ifølge krav 1, hvor den første og den anden sekvens hver har en længde valgt fra gruppen bestående af 20-30 nucleotider, 30-50 nucleotider, 50-100 nucleotider, 100-150 nucleotider, 150-200 nucleotider, 200-300 nucleotider, 300-400 nucleotider, 400-500 nucleotider, 500-600 nucleotider og 600-700 nucleotider.
4. Ekspressionsvektor omfattende en promotor anbragt opstrøms og operativt koblet til NtHMA-RNAi-konstruktet som defineret i et hvilket som helst af kravene 1-3.
5. Ekspressionsvektor ifølge krav 4, hvor promotoren er valgt fra gruppen bestående af: en vævsspecifik promotor, en inducerbar promotor og en konstitutiv promotor.
6. Transgen plante omfattende RNAi'en ifølge et hvilket som helst af kravene 1-3, der har mindskede Cd-niveauer i mindst en del af planten sammenlignet med delen i et ik-ke-transgent modstykke.
7. Transgen plante ifølge krav 6, hvor den transgene plante er en tobaksplante.
8. Konsumerbart tobaksprodukt omfattende blade omfattende RNAi'en ifølge et hvilket som helst af kravene 1-3 og høstet fra den transgene plante ifølge krav 6, således at bladene har mindsket Cd sammenlignet med blade høstet fra et ikke-transgent modstykke dyrket under identiske betingelser, hvor produktet har mindskede Cd-niveauer sammenlignet med et produkt omfattende blade høstet fra det ikke-transgene modstykke, og hvor produktet er en cigar, en cigaret eller et røgfrit tobaksprodukt.
9. Produkt ifølge krav 8, hvor en % Cd-mindskelse er en værdi af mindst cirka 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% eller 99%.
10. Produkt ifølge krav 8 eller krav 9, hvor Cd-indholdet er en værdi i området fra cirka 0,01 til cirka 0,05 ppm, fra cirka 0,01 til cirka 0,1 ppm, fra cirka 0,01 til cirka 0,5 ppm, fra cirka 0,01 til cirka 1,0 ppm eller fra cirka 0,01 til cirka 5 ppm.
11. Fremgangsmåde til mindskelse af Cd-niveauer i mindst en del af en plante, omfattende: mindskelse af niveauer af en NtHMA-mRNA i planten ved at forårsage ekspression af et RNAi-konstrukt, hvor NtHMA'et er valgt fra gruppen bestående af: et polynucleotid omfattende eller bestående af en sekvens, der har mindst 70% sekvensidentitet med SEQ ID NO:l, og som koder for en NtHMA-transporter, der har PI B-type 3 ATPase-aktivitet; et polynucleotid omfattende eller bestående af en sekvens, der har mindst 50% sekvensidentitet med SEQ ID NO: 3; et polynucleotid omfattende eller bestående af en sekvens, der har mindst 50% sekvensidentitet med SEQ ID NO: 47; et polynucleotid omfattende eller bestående af en sekvens kodende for et polypeptid, der har mindst 70% sekvensidentitet med SEQ ID NO:2, hvor polypeptidet er en NtHMA-transporter, der har PIB-type ATPase-aktivitet og et polynucleotid omfattende eller bestående af en sekvens kodende for et polypeptid, der har mindst 70% sekvensidentitet med SEQ ID NO: 49, hvor polypeptidet er en NtHMA-transporter, der har PIB-type ATPase-aktivitet.
12. Fremgangsmåde ifølge krav 11, hvor RNAi-konstruktet omfatter: en første sekvens, der har mindst 90% sekvensidentitet med en del af SEQ ID NO:3 eller 47; en anden sekvens og en tredje sekvens, der har en omvendt komplementær sekvens af den første sekvens, anbragt i samme orientering som den første sekvens, hvor den anden sekvens er anbragt mellem den første sekvens og den tredje sekvens, og den anden sekvens er operativt koblet til den første sekvens og til den tredje sekvens.
13. Fremgangsmåde ifølge krav 11 eller krav 12, hvor, efter ekspression af RNAi-konstruktet, delen af planten har et Cd-indhold mindsket med mindst cirka 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% eller 99%.
14. Fremgangsmåde ifølge et hvilket som helst af kravene 11 til 13, hvor, efter ekspression af RNAi-konstruktet, delen af planten har et Cd-indhold i området fra cirka 0,01 til cirka 0,05 ppm, fra cirka 0,01 til cirka 0,1 ppm, fra cirka 0,01 til cirka 0,5 ppm, fra cirka 0,01 til cirka 1,0 ppm eller fra cirka 0,01 til cirka 5 ppm.
DK08860815.3T 2007-12-13 2008-12-11 Transgene planter modificeret for mindsket cadmiumtransport, afledte produkter og beslægtede fremgangsmåder. DK2231861T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US99698207P 2007-12-13 2007-12-13
PCT/EP2008/010546 WO2009074325A1 (en) 2007-12-13 2008-12-11 Transgenic plants modified for reduced cadmium transport, derivative products, and related methods

Publications (1)

Publication Number Publication Date
DK2231861T3 true DK2231861T3 (da) 2015-01-19

Family

ID=40481764

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08860815.3T DK2231861T3 (da) 2007-12-13 2008-12-11 Transgene planter modificeret for mindsket cadmiumtransport, afledte produkter og beslægtede fremgangsmåder.

Country Status (11)

Country Link
US (4) US8383889B2 (da)
EP (3) EP2559766B1 (da)
CN (2) CN104131030A (da)
AP (1) AP3226A (da)
CO (1) CO6280552A2 (da)
DK (1) DK2231861T3 (da)
ES (2) ES2527272T3 (da)
PL (2) PL2559766T3 (da)
PT (1) PT2231861E (da)
SI (1) SI2231861T1 (da)
WO (1) WO2009074325A1 (da)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6730832B1 (en) 2001-09-10 2004-05-04 Luis Mayan Dominguez High threonine producing lines of Nicotiana tobacum and methods for producing
EP2559766B1 (en) 2007-12-13 2017-10-18 Philip Morris Products S.A. Plants modified for reduced cadmium transport, derivative products, and related methods
US8257401B2 (en) * 2008-02-12 2012-09-04 Spinal U.S.A. Bottom mounted pedical screw assembly
ES2698154T3 (es) 2010-09-03 2019-01-31 Philip Morris Products Sa Reducción de metales pesados en plantas
WO2012041913A1 (en) 2010-09-30 2012-04-05 Societé Nationale D'exploitation Industrielle Des Tabacs Et Allumettes - S.E.I.T.A. Tobacco with reduced cadmium content
CN102653761A (zh) * 2011-03-04 2012-09-05 中国农业科学院植物保护研究所 一种利用RNAi培育抗棉铃虫植物的方法
EP2565271A1 (en) 2011-09-02 2013-03-06 Philip Morris Products S.A. Threonine synthase from Nicotiana tabacum and methods and uses thereof
EP2565265A1 (en) 2011-09-02 2013-03-06 Philip Morris Products S.A. Isopropylmalate synthase from Nicotiana tabacum and methods and uses thereof
US8716571B2 (en) 2011-09-21 2014-05-06 Reynolds Technologies, Inc. Tobacco having reduced amounts of amino acids and methods for producing such lines
EP2773658B1 (en) 2011-10-31 2018-05-16 Philip Morris Products S.a.s. Modulating beta-damascenone in plants
EP2586792A1 (en) 2011-10-31 2013-05-01 Philip Morris Products S.A. Modulating beta-damascenone in plants
CN104470353B (zh) * 2012-02-08 2018-02-13 雷诺兹科技股份有限公司 具有可变量的环境污染物的烟草和用于制造这种品系的方法
US9137958B2 (en) 2012-02-08 2015-09-22 Reynolds Technologies, Inc. Tobacco having altered amounts of environmental contaminants
WO2014096283A2 (en) 2012-12-21 2014-06-26 Philip Morris Products S.A Tobacco specific nitrosamine reduction in plants
JP6871158B2 (ja) 2014-05-08 2021-05-12 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物体におけるニコチンからノルニコチンへの変換の低減
CN103981194B (zh) * 2014-06-10 2016-08-24 云南省烟草农业科学研究院 一种烟草镉转运基因NtHMA2及其克隆方法与应用
CA3010142A1 (en) 2016-01-29 2017-08-03 Philip Morris Products S.A. Reducing cadmium accumulation in field grown tobacco plants
WO2018114641A1 (en) 2016-12-20 2018-06-28 Philip Morris Products S.A. Plants with shortened time to flowering
EP3480314A1 (en) 2017-11-03 2019-05-08 Philip Morris Products S.A. Regulation of alkaloid content
JP2021519064A (ja) 2018-03-28 2021-08-10 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物体における還元糖含有量の調節
WO2019185703A1 (en) 2018-03-28 2019-10-03 Philip Morris Products S.A. Modulating amino acid content in a plant
US11053526B2 (en) 2018-08-09 2021-07-06 Evonik Operations Gmbh Process for preparing L amino acids using improved strains of the enterobacteriaceae family
BR112021012596A2 (pt) 2018-12-30 2021-11-30 Philip Morris Products Sa Produtos de tabaco compreendendo uma célula vegetal, parte de uma planta ou um material vegetal, método para produzir uma planta e método para produzir material vegetal curado
WO2021063863A1 (en) 2019-10-01 2021-04-08 Philip Morris Products S.A. Modulating sugar and amino acid content in a plant (sultr3)
JP2022550383A (ja) 2019-10-01 2022-12-01 フィリップ・モーリス・プロダクツ・ソシエテ・アノニム 植物における還元糖含有量の調節（ｉｎｖ）
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
WO2024079137A1 (en) 2022-10-13 2024-04-18 Philip Morris Products S.A. Increasing leaf biomass and nitrogen use efficiency by regulating ntp2

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4376110A (en) 1980-08-04 1983-03-08 Hybritech, Incorporated Immunometric assays using monoclonal antibodies
US4469863A (en) 1980-11-12 1984-09-04 Ts O Paul O P Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof
US4459355A (en) 1982-07-12 1984-07-10 International Paper Company Method for transforming plant cells
NL8300698A (nl) 1983-02-24 1984-09-17 Univ Leiden Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten.
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5235033A (en) 1985-03-15 1993-08-10 Anti-Gene Development Group Alpha-morpholino ribonucleoside derivatives and polymers thereof
US5034506A (en) 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
US4795855A (en) 1985-11-14 1989-01-03 Joanne Fillatti Transformation and foreign gene expression with woody species
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5216141A (en) 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
US5602240A (en) 1990-07-27 1997-02-11 Ciba Geigy Ag. Backbone modified oligonucleotide analogs
US5386023A (en) 1990-07-27 1995-01-31 Isis Pharmaceuticals Backbone modified oligonucleotide analogs and preparation thereof through reductive coupling
US5644048A (en) 1992-01-10 1997-07-01 Isis Pharmaceuticals, Inc. Process for preparing phosphorothioate oligonucleotides
GB9324707D0 (en) 1993-12-02 1994-01-19 Olsen Odd Arne Promoter
US5637684A (en) 1994-02-23 1997-06-10 Isis Pharmaceuticals, Inc. Phosphoramidate and phosphorothioamidate oligomeric compounds
US6586661B1 (en) * 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
CA2315549A1 (en) * 1998-02-26 1999-09-02 Pioneer Hi-Bred International, Inc. Family of maize pr-1 genes and promoters
JP3507856B2 (ja) 1998-06-10 2004-03-15 九州大学長 Ｄｎａフラグメント、組み換えｄｎａ、形質転換植物
ATE309362T1 (de) 1998-08-20 2005-11-15 Pioneer Hi Bred Int Samen-bevorzugende promotoren
AU5788299A (en) 1998-08-28 2000-03-21 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from (end) genes
US6403862B1 (en) 1999-09-24 2002-06-11 Pioneer Hi-Bred International, Inc. Seed-preferred promoter from maize
US6407315B1 (en) 1999-11-02 2002-06-18 Pioneer Hi-Bred International, Inc. Seed-preferred promoter from barley
US7122658B1 (en) * 2000-11-22 2006-10-17 Pioneer Hi-Bred International, Inc. Seed-preferred regulatory elements and uses thereof
EP1373523B1 (en) * 2001-04-04 2009-08-05 Posco Genetic modification of plants for enhanced resistance and decreased uptake of heavy metals
WO2005093078A1 (en) * 2004-03-18 2005-10-06 Commissariat A L'energie Atomique Genetically modified plants and their applications in phytoremediation.
EP2559766B1 (en) * 2007-12-13 2017-10-18 Philip Morris Products S.A. Plants modified for reduced cadmium transport, derivative products, and related methods

Also Published As

Publication number Publication date
EP2231861A1 (en) 2010-09-29
ES2527272T3 (es) 2015-01-22
US8829161B2 (en) 2014-09-09
SI2231861T1 (sl) 2015-02-27
PL2231861T3 (pl) 2015-04-30
US20090183280A1 (en) 2009-07-16
BRPI0820741A2 (pt) 2015-04-14
US20130183738A1 (en) 2013-07-18
PL2559766T3 (pl) 2018-02-28
CN101952442A (zh) 2011-01-19
WO2009074325A1 (en) 2009-06-18
AP3226A (en) 2015-04-30
EP2559766A1 (en) 2013-02-20
EP2231861B1 (en) 2014-10-29
AP2010005311A0 (en) 2010-08-31
EP3327129A1 (en) 2018-05-30
PT2231861E (pt) 2015-02-03
CN104131030A (zh) 2014-11-05
EP2559766B1 (en) 2017-10-18
US20170218385A1 (en) 2017-08-03
US20140378667A1 (en) 2014-12-25
US8383889B2 (en) 2013-02-26
US10287598B2 (en) 2019-05-14
ES2645763T3 (es) 2017-12-07
US9644206B2 (en) 2017-05-09
CO6280552A2 (es) 2011-05-20

Similar Documents

Publication Publication Date Title
DK2231861T3 (da) Transgene planter modificeret for mindsket cadmiumtransport, afledte produkter og beslægtede fremgangsmåder.
KR102442802B1 (ko) 식물 내 니코틴의 노르니코틴으로의 변환 감소
KR102136088B1 (ko) 식물계에서의 중금속 감소
JP6302407B2 (ja) 植物におけるβ−ダマセノンの調節
KR102012993B1 (ko) 니코티아나 타바쿰으로부터의 이소프로필말레이트 합성효소 및 그의 방법 및 용도
KR20140057642A (ko) 니코티아나 타바쿰으로부터의 트레오닌 합성효소 및 그의 방법 및 용도
KR20190094161A (ko) 개화까지의 시간이 단축된 식물
US9187757B2 (en) Isolation and targeted suppression of lignin biosynthetic genes
BRPI0820741B1 (pt) MÉTODO PARA REDUZIR OS NÍVEIS DE Cd EM UMA PLANTA DE TABACO
BR122018008802B1 (pt) Produto de tabaco consumível compreendendo folhas de planta de tabaco modificada com níveis de cádmio reduzidos
EP2586792A1 (en) Modulating beta-damascenone in plants