DK2220229T3 - Mutant g-protein coupled receptors and methods for selecting them - Google Patents

Mutant g-protein coupled receptors and methods for selecting them Download PDF

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Publication number
DK2220229T3
DK2220229T3 DK08856833.2T DK08856833T DK2220229T3 DK 2220229 T3 DK2220229 T3 DK 2220229T3 DK 08856833 T DK08856833 T DK 08856833T DK 2220229 T3 DK2220229 T3 DK 2220229T3
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Prior art keywords
mutant
selecting
methods
protein coupled
coupled receptors
Prior art date
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DK08856833.2T
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Danish (da)
English (en)
Inventor
Jonathan Richard Heal
Richard Henderson
Christopher Gordon Tate
Malcolm Peter Weir
Original Assignee
Heptares Therapeutics Ltd
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Publication of DK2220229T3 publication Critical patent/DK2220229T3/da

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Ecology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
DK08856833.2T 2007-12-08 2008-12-08 Mutant g-protein coupled receptors and methods for selecting them DK2220229T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0724051.8A GB0724051D0 (en) 2007-12-08 2007-12-08 Mutant proteins and methods for producing them
PCT/GB2008/004032 WO2009071914A2 (en) 2007-12-08 2008-12-08 Mutant proteins and methods for producing them

Publications (1)

Publication Number Publication Date
DK2220229T3 true DK2220229T3 (da) 2020-09-07

Family

ID=38983182

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08856833.2T DK2220229T3 (da) 2007-12-08 2008-12-08 Mutant g-protein coupled receptors and methods for selecting them

Country Status (10)

Country Link
US (1) US8748182B2 (enExample)
EP (2) EP2220229B1 (enExample)
JP (2) JP2011505800A (enExample)
AU (1) AU2008332896B2 (enExample)
CA (1) CA2708037C (enExample)
DK (1) DK2220229T3 (enExample)
ES (1) ES2972473T3 (enExample)
GB (2) GB0724051D0 (enExample)
PL (1) PL2568045T3 (enExample)
WO (1) WO2009071914A2 (enExample)

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CN101688203B (zh) 2007-03-22 2013-12-11 赫普泰雅治疗有限公司 突变的g蛋白偶联受体及其选择方法
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US10373702B2 (en) 2014-03-27 2019-08-06 Massachusetts Institute Of Technology Water-soluble trans-membrane proteins and methods for the preparation and use thereof
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Also Published As

Publication number Publication date
WO2009071914A2 (en) 2009-06-11
EP2220229B1 (en) 2020-06-24
EP2568045A1 (en) 2013-03-13
CA2708037C (en) 2018-09-18
JP2011505800A (ja) 2011-03-03
EP2220229A2 (en) 2010-08-25
GB2467830A (en) 2010-08-18
ES2972473T3 (es) 2024-06-12
US8748182B2 (en) 2014-06-10
US20110028700A1 (en) 2011-02-03
PL2568045T3 (pl) 2024-05-13
AU2008332896B2 (en) 2014-05-08
CA2708037A1 (en) 2009-06-11
JP2015133973A (ja) 2015-07-27
EP2568045B1 (en) 2024-01-24
JP6567291B2 (ja) 2019-08-28
AU2008332896A1 (en) 2009-06-11
WO2009071914A3 (en) 2009-10-15
GB0724051D0 (en) 2008-01-16
GB2467830B (en) 2013-05-15
GB201000387D0 (en) 2010-02-24

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