AU2008332896B2 - Mutant proteins and methods for producing them - Google Patents

Mutant proteins and methods for producing them Download PDF

Info

Publication number
AU2008332896B2
AU2008332896B2 AU2008332896A AU2008332896A AU2008332896B2 AU 2008332896 B2 AU2008332896 B2 AU 2008332896B2 AU 2008332896 A AU2008332896 A AU 2008332896A AU 2008332896 A AU2008332896 A AU 2008332896A AU 2008332896 B2 AU2008332896 B2 AU 2008332896B2
Authority
AU
Australia
Prior art keywords
gpcr
mutant
amino acid
parent
receptor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2008332896A
Other languages
English (en)
Other versions
AU2008332896A1 (en
Inventor
Jonathan Richard Heal
Richard Henderson
Christopher Gordon Tate
Malcolm Peter Weir
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nxera Pharma UK Ltd
Original Assignee
Nxera Pharma UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nxera Pharma UK Ltd filed Critical Nxera Pharma UK Ltd
Publication of AU2008332896A1 publication Critical patent/AU2008332896A1/en
Application granted granted Critical
Publication of AU2008332896B2 publication Critical patent/AU2008332896B2/en
Assigned to NXERA PHARMA UK LIMITED reassignment NXERA PHARMA UK LIMITED Request to Amend Deed and Register Assignors: HEPTARES THERAPEUTICS LIMITED
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Ecology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
AU2008332896A 2007-12-08 2008-12-08 Mutant proteins and methods for producing them Active AU2008332896B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0724051.8A GB0724051D0 (en) 2007-12-08 2007-12-08 Mutant proteins and methods for producing them
GB0724051.8 2007-12-08
PCT/GB2008/004032 WO2009071914A2 (en) 2007-12-08 2008-12-08 Mutant proteins and methods for producing them

Publications (2)

Publication Number Publication Date
AU2008332896A1 AU2008332896A1 (en) 2009-06-11
AU2008332896B2 true AU2008332896B2 (en) 2014-05-08

Family

ID=38983182

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2008332896A Active AU2008332896B2 (en) 2007-12-08 2008-12-08 Mutant proteins and methods for producing them

Country Status (10)

Country Link
US (1) US8748182B2 (enExample)
EP (2) EP2220229B1 (enExample)
JP (2) JP2011505800A (enExample)
AU (1) AU2008332896B2 (enExample)
CA (1) CA2708037C (enExample)
DK (1) DK2220229T3 (enExample)
ES (1) ES2972473T3 (enExample)
GB (2) GB0724051D0 (enExample)
PL (1) PL2568045T3 (enExample)
WO (1) WO2009071914A2 (enExample)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101688203B (zh) 2007-03-22 2013-12-11 赫普泰雅治疗有限公司 突变的g蛋白偶联受体及其选择方法
GB0724051D0 (en) 2007-12-08 2008-01-16 Medical Res Council Mutant proteins and methods for producing them
GB0724860D0 (en) * 2007-12-20 2008-01-30 Heptares Therapeutics Ltd Screening
GB0802474D0 (en) 2008-02-11 2008-03-19 Heptares Therapeutics Ltd Mutant proteins and methods for selecting them
US20110112037A1 (en) * 2008-03-05 2011-05-12 Heptares Therapeutics Limited BioPark Crystal structure
GB0910725D0 (en) 2009-06-22 2009-08-05 Heptares Therapeutics Ltd Mutant proteins and methods for producing them
EP3333185A1 (en) * 2010-08-20 2018-06-13 Heptares Therapeutics Limited Mutant gpcrs
US20140031525A1 (en) 2011-01-21 2014-01-30 Heptares Therapeutics Limited Mutant g-protein coupled receptor proteins and methods for producing them
CA2827187C (en) 2011-02-23 2019-01-08 Massachusetts Institute Of Technology Water soluble membrane proteins and methods for the preparation and use thereof
WO2012120315A2 (en) 2011-03-10 2012-09-13 Heptares Therapeutics Limited Mutant proteins and methods for producing them
CN107739408A (zh) 2011-08-10 2018-02-27 赫普泰雅治疗有限公司 稳定的蛋白质
ES2676827T3 (es) 2012-06-01 2018-07-25 Heptares Therapeutics Limited Ensayos
CU24181B1 (es) * 2012-11-09 2016-04-25 Ct De Inmunología Molecular POLIPÉPTIDOS DERIVADOS DEL TGFß
CN105102478A (zh) 2013-01-30 2015-11-25 弗拉芒区生物技术研究所 用于筛选和药物发现目的的新型嵌合多肽
WO2014140586A2 (en) 2013-03-15 2014-09-18 Medical Research Council Mutant proteins and methods for their production
US10373702B2 (en) 2014-03-27 2019-08-06 Massachusetts Institute Of Technology Water-soluble trans-membrane proteins and methods for the preparation and use thereof
WO2016070022A1 (en) 2014-10-31 2016-05-06 Abilita Bio, Inc. Modified membrane spanning proteins and methods for the preparation and use thereof
EP3344043A4 (en) * 2015-09-04 2019-07-10 The Scripps Research Institute METHOD FOR IDENTIFYING NEW ANTIBIOTICS AND RELATED COMPOSITIONS
GB201601690D0 (en) 2016-01-29 2016-03-16 Heptares Therapeutics Ltd G proteins
SMT201900460T1 (it) 2016-02-29 2019-09-09 Ose Immunotherapeutics Anticorpi non antagonisti diretti contro la catena alfa del dominio extracellulare del recettore di il17 e loro uso nel trattamento del cancro
US10845367B2 (en) 2016-05-04 2020-11-24 Abilita Bio, Inc. Modified multispanning membrane polypeptides and methods of use thereof to screen therapeutic agents
WO2018150260A1 (en) * 2017-02-16 2018-08-23 Institut Pasteur Method for the identification of mutated proteins having modified thermal stability

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020147170A1 (en) * 2000-10-26 2002-10-10 Kopin Alan S. Constitutively active, hypersensitive, and nonfunctional recepors as novel therapeutic agents
WO2008114020A2 (en) * 2007-03-22 2008-09-25 Heptares Therapeutics Limited Mutant g-protein coupled receptors and methods for selecting them

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4816567A (en) * 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
JPS61134325A (ja) 1984-12-04 1986-06-21 Teijin Ltd ハイブリツド抗体遺伝子の発現方法
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US4946778A (en) * 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US6780613B1 (en) * 1988-10-28 2004-08-24 Genentech, Inc. Growth hormone variants
ATE189526T1 (de) 1988-10-28 2000-02-15 Genentech Inc Verfahren zum nachweis von aktiven domänen und aminosäureresten in polypeptiden und hormonvarianten
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
AU665190B2 (en) 1990-07-10 1995-12-21 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
WO1992009690A2 (en) 1990-12-03 1992-06-11 Genentech, Inc. Enrichment method for variant proteins with altered binding properties
ATE439435T1 (de) 1991-03-01 2009-08-15 Dyax Corp Chimäres protein mit mikroprotein mit zwei oder mehr disulfidbindungen und ausgestaltungen davon
JP3147916B2 (ja) * 1991-03-08 2001-03-19 森永製菓株式会社 体外免疫方法
DE69233750D1 (de) 1991-04-10 2009-01-02 Scripps Research Inst Bibliotheken heterodimerer Rezeptoren mittels Phagemiden
DE4122599C2 (de) 1991-07-08 1993-11-11 Deutsches Krebsforsch Phagemid zum Screenen von Antikörpern
US20030036092A1 (en) * 1991-11-15 2003-02-20 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
US5585277A (en) * 1993-06-21 1996-12-17 Scriptgen Pharmaceuticals, Inc. Screening method for identifying ligands for target proteins
JPH10500951A (ja) 1994-05-23 1998-01-27 スミスクライン・ビーチャム・コーポレイション コードされた組合せライブラリー
US20020028443A1 (en) * 1999-09-27 2002-03-07 Jay M. Short Method of dna shuffling with polynucleotides produced by blocking or interrupting a synthesis or amplification process
AU2020497A (en) 1996-03-27 1997-10-17 Susan R. George Receptor and transporter antagonists
AU2728297A (en) * 1996-04-15 1997-11-07 Board Of Trustees Of The Leland Stanford Junior University Soluble 7-transmembrane domain G-protein-coupled receptor compositions and me thods
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
US6555339B1 (en) 1997-04-14 2003-04-29 Arena Pharmaceuticals, Inc. Non-endogenous, constitutively activated human protein-coupled receptors
CA2323638A1 (en) * 1998-04-03 1999-10-14 Phylos, Inc. Addressable protein arrays
US7094593B1 (en) * 1998-09-01 2006-08-22 Basf Aktiengesellschaft Method for improving the function of heterologous G protein-coupled receptors
IL149569A0 (en) 1999-11-17 2002-11-10 Arena Pharm Inc Endogenous and non-endogenous versions of human g protein-coupled receptors
AU2001286945A1 (en) * 2000-08-30 2002-03-13 John Hopkins University School Of Medicine Identification of activated receptors and ion channels
US6448377B1 (en) * 2000-09-27 2002-09-10 The Board Of Trustees Of The Leland Stanford Junior University Modified G protein sunbunits
CN1516706A (zh) 2001-02-26 2004-07-28 阿伦纳药品公司 人g蛋白偶联受体的内源性和非内源性形式
US7208279B2 (en) * 2001-03-14 2007-04-24 Caden Biosciences, Inc. Method for identifying inhibitors of G protein coupled receptor signaling
JPWO2002079784A1 (ja) * 2001-03-30 2004-07-22 サントリー株式会社 Ｇ蛋白質結合型レセプターの構造モデルおよび当該構造モデルを用いたｇ蛋白質結合型レセプターに結合するリガンドの設計方法
US20030129649A1 (en) * 2001-04-24 2003-07-10 Kobilka Brian K. Conformational assays to detect binding to G protein-coupled receptors
EP1438337B1 (en) 2001-10-25 2006-09-20 Astex Therapeutics Limited Crystals of cytochrome p450 2c9, structures thereof and their use
US7115377B2 (en) * 2001-10-26 2006-10-03 Atto Bioscience, Inc. Cell-based assays for G-protein-coupled receptor-mediated activities
EP1505074A4 (en) 2002-04-24 2005-12-21 Banyu Pharma Co Ltd CONSTITUTATIVE ACTIVE MUTANTS OF A HISTAMINE H3 RECEPTOR AND ITS USES
ATE457728T1 (de) * 2002-05-01 2010-03-15 Vertex Pharma Kristallstruktur des aurora-2 proteins und dessen bindungstaschen
US7745161B2 (en) * 2003-12-19 2010-06-29 Palo Alto Research Center Incorporated Amplification of enzymatic reactions for use with an enthalpy array
US20050287121A1 (en) * 2004-05-12 2005-12-29 Merchiers Pascal G Methods, compositions and compound assays for inhibiting amyloid-beta protein production
WO2005121755A1 (en) 2004-06-14 2005-12-22 Commonwealth Scientific And Industrial Research Organisation Cell free g-protein coupled receptor and ligand assay
US20060188964A1 (en) 2004-07-28 2006-08-24 Filippo Mancia Processing for producing and crystallizing G-protein coupled receptors
US20070154947A1 (en) * 2005-08-31 2007-07-05 The Trustees Of Princeton University Chemical biodiscriminator
WO2007097820A2 (en) * 2005-11-18 2007-08-30 The Ohio State University Research Foundation Viral gene products and methods for vaccination to prevent viral associated diseases
GB0724051D0 (en) 2007-12-08 2008-01-16 Medical Res Council Mutant proteins and methods for producing them
GB0724860D0 (en) * 2007-12-20 2008-01-30 Heptares Therapeutics Ltd Screening
GB0802474D0 (en) 2008-02-11 2008-03-19 Heptares Therapeutics Ltd Mutant proteins and methods for selecting them
US20110112037A1 (en) * 2008-03-05 2011-05-12 Heptares Therapeutics Limited BioPark Crystal structure
CN102046684B (zh) * 2008-05-30 2013-01-16 旭硝子株式会社 硬质发泡合成树脂及其制造方法
GB0910725D0 (en) * 2009-06-22 2009-08-05 Heptares Therapeutics Ltd Mutant proteins and methods for producing them
EP3333185A1 (en) * 2010-08-20 2018-06-13 Heptares Therapeutics Limited Mutant gpcrs

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020147170A1 (en) * 2000-10-26 2002-10-10 Kopin Alan S. Constitutively active, hypersensitive, and nonfunctional recepors as novel therapeutic agents
WO2008114020A2 (en) * 2007-03-22 2008-09-25 Heptares Therapeutics Limited Mutant g-protein coupled receptors and methods for selecting them

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
D'Antona AM et al, Brain Research, 2006, 1108(1):1-11 *

Also Published As

Publication number Publication date
WO2009071914A2 (en) 2009-06-11
EP2220229B1 (en) 2020-06-24
EP2568045A1 (en) 2013-03-13
CA2708037C (en) 2018-09-18
JP2011505800A (ja) 2011-03-03
EP2220229A2 (en) 2010-08-25
GB2467830A (en) 2010-08-18
ES2972473T3 (es) 2024-06-12
US8748182B2 (en) 2014-06-10
US20110028700A1 (en) 2011-02-03
PL2568045T3 (pl) 2024-05-13
CA2708037A1 (en) 2009-06-11
JP2015133973A (ja) 2015-07-27
DK2220229T3 (da) 2020-09-07
EP2568045B1 (en) 2024-01-24
JP6567291B2 (ja) 2019-08-28
AU2008332896A1 (en) 2009-06-11
WO2009071914A3 (en) 2009-10-15
GB0724051D0 (en) 2008-01-16
GB2467830B (en) 2013-05-15
GB201000387D0 (en) 2010-02-24

Similar Documents

Publication Publication Date Title
AU2008332896B2 (en) Mutant proteins and methods for producing them
US20240052017A1 (en) Mutant g-protein coupled receptors and methods for selecting them
AU2012200841B2 (en) Mutant proteins and methods for selecting them
GB2456904A (en) Mutant neurotensin receptors with improved stability

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
HB Alteration of name in register

Owner name: NXERA PHARMA UK LIMITED

Free format text: FORMER NAME(S): HEPTARES THERAPEUTICS LIMITED