DK2173864T3 - Gel med ringe stivhed til anvendelse ved MSC-vækstmodulation - Google Patents
Gel med ringe stivhed til anvendelse ved MSC-vækstmodulation Download PDFInfo
- Publication number
- DK2173864T3 DK2173864T3 DK08779877.3T DK08779877T DK2173864T3 DK 2173864 T3 DK2173864 T3 DK 2173864T3 DK 08779877 T DK08779877 T DK 08779877T DK 2173864 T3 DK2173864 T3 DK 2173864T3
- Authority
- DK
- Denmark
- Prior art keywords
- another embodiment
- cell
- cells
- stem cell
- gel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0068—General culture methods using substrates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/52—Fibronectin; Laminin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Developmental Biology & Embryology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Rheumatology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
- Investigating Strength Of Materials By Application Of Mechanical Stress (AREA)
- Peptides Or Proteins (AREA)
Claims (13)
1. Fremgangsmåde til induktion eller opretholdelse af hviletilstanden i en mesenchymal stamcelle såvel som opretholdelse af cellens evne, ex vivo, til genoptagelse af vækst samt påbegyndelse af differentiering, omfattende: at kontakte den mesenchymale stamcelle med en gel eller gelmatrix, som er belagt med et adhæsionsprotein, som binder til integrinet på den mesenchymale stamcelles membran, hvilken gel eller gelmatrix har et forskydningselasticitetsmodul i intervallet 150-750 Pa; og forsyning af den mesenchymale stamcelle med næringsmateriale.
2. Fremgangsmåde ifølge krav 1, hvorved gelen eller gelmatrixen er 2-dimensional eller 3-dimensional.
3. Fremgangsmåde ifølge krav 1, hvorved den mesenchymale stamcelle udvælges fra gruppen, som består af en fra knoglemarv udvundet mesenchymal stamcelle (MSC), en renal stamcelle, en fra skeletmuskler udvundet MSC, en fra knogler udvundet MSC, en fra tandkød udvundet MSC, en fra hjertemuskel udvundet MSC, en fra synovialvæske udvundet MSC, en fra navlesnoren udvundet MSC og en fra adipøst væv udvundet MSC.
4. Fremgangsmåde ifølge krav 1, hvorved den mesenchymale stamcelle før kontaktskabelsen ikke er i hviletilstand.
5. Fremgangsmåde til induktion af formering ex vivo i en mesenchymal stamcelle, omfattende trinnene at realisere fremgangsmåde ifølge krav 1, bringe den mesenchymale stamcelle ex vivo i kontakt med et formerings-inducerende materiale, der omfatter en gel eller gelmatrix, som er belagt med et adhæsionsprotein, der hæfter til integrin på overfladen af den mesenchymale stamcelle, hvilken gel eller gelmatrix udviser et forskydningselasticitetsmodul, som er 7500 Pa; og tilvejebringelse af nærings-vækstmateriale til fremme af formering af den mesenchymale stamcelle og dens afkom ex vivo.
6. Fremgangsmåde til induktion af differentiering ex vivo i en mesenchymal stamcelle, omfattende trinnene: realisering af fremgangsmåde ifølge krav 1 eller realisering af fremgangsmåden ifølge krav 5; dannelse af kontakt mellem den mesenchymale stamcelle og et differentieringsmateriale, omfattende mekaniske og/eller kemiske stimuli, som er udvalgt til at stimulere differentiering af den mesenchymale stamcelle til en forudbestemt celletype; og forsyning af den differentierede celle med differentieret-celle næringsmiddelmateriale.
7. Fremgangsmåde ifølge krav 6, hvorved den mesenchymale stamcelle er en af human knoglemarv udvundet mesenchymal stamcelle, og hvorved den differentierede celle er en osteoblast, en chondrocyt, en myocyt, en beta-pancreatic ø-celle, en neuronal celle eller en bindevævscelle.
8. Fremgangsmåde ifølge krav 1 eller 5, hvorved (a) gelen eller gelmatrixen omfatter acrylamid og bisacrylamid; og/eller (b) adhæsionsproteinet er et kollagen, et fibronektin eller en kombination af disse.
9. Fremgangsmåde til konservering af en population af mesenchymale stamceller, hvorved fremgangsmåden omfatter trinnet med dyrkning af populationen af mesenchymale stamceller på en gel eller en gelmatrix med et forskydningselasticitetsmodul i intervallet mellem 150 Pa og 750 Pa.
10. Fremgangsmåde ifølge krav 9, hvorved gelen eller gelen i gelmatrixen omfatter et geleringsmiddel og en blanding af acrylamid og bisacrylamid, og hvorved gelen eller gelmatrixen er belagt med et adhæsionsprotein.
11. Fremgangsmåde ifølge krav 10, hvorved adhæsionsproteinet er et kollagen, et fibronectin eller en kombination deraf.
12. Fremgangsmåde ifølge krav 9, hvorved (a) telomer-længden i den mesenchymale stamcelepopulation opretholdes; og/eller (b) den mesenchymale stamcellepopulation opretholdes i en hviletilstand; og/eller (c) den mesenchymale stamcellepopulation bibeholder en evne til at differentiere til en celletype, som er udvalgt fra en adipozyt og en osteoblast, og/eller (d) gelen eller gelmatrixen ydermere omfatter et animalsk serum; og/eller (e) trinnet med dyrkning realiseres direkte efter isolation, rensning eller berigelse af den mesenchymale stamcellepopulation i et vævsdyrkningsapparat; og/eller (f) trinnet med dyrkning realiseres direkte efter isolering, rensning eller berigelse af den mesenchymale stamcellepopulation fra en biologisk prøve.
13. Fremgangsmåde ifølge krav 9, ydermere omfattende trinnet med efterfølgende plettering af den mesenchymale stamcellepopulation i et vævsdyrkningsapparat.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US92948907P | 2007-06-29 | 2007-06-29 | |
| US92948707P | 2007-06-29 | 2007-06-29 | |
| US96007007P | 2007-09-14 | 2007-09-14 | |
| PCT/US2008/008120 WO2009005770A1 (en) | 2007-06-29 | 2008-06-30 | Low rigidity gels for msc growth modulation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2173864T3 true DK2173864T3 (da) | 2016-08-22 |
Family
ID=40226420
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK08779877.3T DK2173864T3 (da) | 2007-06-29 | 2008-06-30 | Gel med ringe stivhed til anvendelse ved MSC-vækstmodulation |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10214720B2 (da) |
| EP (2) | EP3034606A1 (da) |
| JP (3) | JP5737936B2 (da) |
| CN (2) | CN106978393A (da) |
| CA (1) | CA2691790C (da) |
| DK (1) | DK2173864T3 (da) |
| HK (2) | HK1143604A1 (da) |
| WO (1) | WO2009005770A1 (da) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2691790C (en) | 2007-06-29 | 2017-05-02 | The Trustees Of The University Of Pennsylvania | Low rigidity gels for msc growth modulation |
| US11083190B2 (en) | 2007-06-29 | 2021-08-10 | Makoto Funaki | Soft gel systems in modulating stem cell development |
| US10328103B2 (en) | 2009-01-03 | 2019-06-25 | Ray C. Wasielewski | Medical treatment composition comprising mammalian dental pulp stem cells |
| US8470308B2 (en) * | 2009-01-03 | 2013-06-25 | Ray C. Wasielewski | Enhanced medical implant comprising disrupted tooth pulp and tooth particles |
| US10130736B1 (en) | 2010-05-14 | 2018-11-20 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US9352003B1 (en) | 2010-05-14 | 2016-05-31 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| US8883210B1 (en) | 2010-05-14 | 2014-11-11 | Musculoskeletal Transplant Foundation | Tissue-derived tissuegenic implants, and methods of fabricating and using same |
| JP5360663B2 (ja) * | 2010-06-29 | 2013-12-04 | 国立大学法人徳島大学 | 培養脂肪細胞 |
| US20130237453A1 (en) * | 2010-10-08 | 2013-09-12 | Ashok C. Chander | Systems, methods and devices for measuring growth/oncogenic and migration/metastatic potential |
| US11007528B2 (en) | 2010-10-08 | 2021-05-18 | Cellanyx Diagnostics, Llc | Systems, methods and devices for measuring growth/oncogenic and migration/metastatic potential |
| WO2014058080A1 (ja) * | 2012-11-30 | 2014-04-17 | 独立行政法人理化学研究所 | 体細胞のリプログラミングを亢進させる方法、及び細胞作製キット |
| CN104046589B (zh) * | 2013-03-11 | 2016-09-14 | 中国科学院大连化学物理研究所 | 一种细胞共培养诱导干细胞体外定向分化的方法 |
| MX2016001247A (es) | 2013-07-30 | 2016-08-17 | Musculoskeletal Transplant Foundation | Matrices derivadas de tejido suave acelular y metodos para preparar las mismas. |
| US10316292B2 (en) | 2014-01-23 | 2019-06-11 | Nissan Chemical Industries, Ltd. | Material for undifferentiated state-maintaining culture |
| EP3297694A1 (en) | 2015-05-21 | 2018-03-28 | Musculoskeletal Transplant Foundation | Modified demineralized cortical bone fibers |
| US10676712B2 (en) * | 2015-07-22 | 2020-06-09 | Inventia Life Science Pty Ltd | Process for printing 3D tissue culture models |
| US10912864B2 (en) | 2015-07-24 | 2021-02-09 | Musculoskeletal Transplant Foundation | Acellular soft tissue-derived matrices and methods for preparing same |
| US11052175B2 (en) | 2015-08-19 | 2021-07-06 | Musculoskeletal Transplant Foundation | Cartilage-derived implants and methods of making and using same |
| CN108159499A (zh) * | 2017-12-06 | 2018-06-15 | 广西医科大学 | 一种复合凝胶及其制备方法和应用 |
| WO2020036918A1 (en) * | 2018-08-15 | 2020-02-20 | Wake Forest University Health Sciences | Improved formulations for pancreatic islet encapsulation |
| TW202104590A (zh) * | 2019-04-10 | 2021-02-01 | 國立大學法人京都大學 | 類生體組織結構體的製造方法 |
| JP7432808B2 (ja) | 2019-05-17 | 2024-02-19 | 東ソー株式会社 | 細胞分化方法 |
| JPWO2022045201A1 (da) * | 2020-08-27 | 2022-03-03 | ||
| EP4238985A4 (en) * | 2020-10-29 | 2024-05-08 | Osaka University | METHOD FOR FREEZING A CELL STRUCTURE |
| EP4363442A1 (en) * | 2021-07-02 | 2024-05-08 | Combangio, Inc. | Processes for making and using a cellular fibronectin composition |
| CN113846054B (zh) * | 2021-09-23 | 2023-05-16 | 四川大学 | 将成纤维细胞直接重编程为成骨细胞的基质材料及制备方法和重编程方法 |
| CA3234969A1 (en) * | 2021-10-12 | 2023-04-20 | Makoto Funaki | Anti-aging composition using mesenchymal stem cells and methods thereof |
| CN114525249A (zh) * | 2022-03-31 | 2022-05-24 | 广西大学 | 一种用于分离牛肌肉干细胞的肌肉组织提取方法及其应用 |
| CN116693931B (zh) * | 2023-07-25 | 2023-10-17 | 中山大学 | 一种超结构多孔湿性粘合水凝胶及其制备方法与应用 |
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| US20020168718A1 (en) | 1997-04-03 | 2002-11-14 | California Institute Of Technology | Enzyme-mediated modification of fibrin for tissue engineering |
| US6991794B1 (en) * | 1997-06-24 | 2006-01-31 | Imclone Systems Incorporated | Progenitor cell preservation factors and methods for and products of their use |
| US6528245B2 (en) | 1998-05-07 | 2003-03-04 | University Of South Florida | Bone marrow cells as a source of neurons for brain and spinal cord repair |
| US6927060B2 (en) * | 2000-03-28 | 2005-08-09 | University Of Iowa Research Foundation | Methods to prepare and use epidermal stem cells |
| US6861255B1 (en) | 2001-07-18 | 2005-03-01 | Sea Run Holdings, Inc. | Method of using fish plasma components for tissue culture |
| EP1403304B1 (en) * | 2001-05-30 | 2008-08-27 | Keiichi Miyamoto | Crosslinked elastin and processes for its production |
| JP2006508682A (ja) * | 2002-12-06 | 2006-03-16 | ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ | β−カテニンシグナル伝達経路の調節による細胞傷害剤からの幹細胞の保護 |
| US20050058687A1 (en) | 2003-09-12 | 2005-03-17 | Becton, Dickinson And Company | Covalently attached collagen VI for cell attachment and proliferation |
| CN1897930A (zh) | 2004-01-30 | 2007-01-17 | 血管技术国际股份公司 | 用于治疗挛缩的组合物和方法 |
| WO2006105278A2 (en) * | 2005-03-29 | 2006-10-05 | The Regents Of The University Of California | Controlling stem cell destiny destiny with tunable network |
| US20070065415A1 (en) * | 2005-09-16 | 2007-03-22 | Kleinsek Donald A | Compositions and methods for the augmentation and repair of defects in tissue |
| JP2007116926A (ja) * | 2005-10-25 | 2007-05-17 | Reprocell Inc | 体外における幹細胞の維持と純化に関する方法、組成物およびシステム |
| US20070190646A1 (en) | 2006-02-10 | 2007-08-16 | The Trustees Of The University Of Pennsylvania | Regulating stem cell differentiation by controlling matrix elasticity |
| CA2691790C (en) | 2007-06-29 | 2017-05-02 | The Trustees Of The University Of Pennsylvania | Low rigidity gels for msc growth modulation |
| US11083190B2 (en) * | 2007-06-29 | 2021-08-10 | Makoto Funaki | Soft gel systems in modulating stem cell development |
-
2008
- 2008-06-30 CA CA2691790A patent/CA2691790C/en active Active
- 2008-06-30 EP EP15196849.2A patent/EP3034606A1/en not_active Withdrawn
- 2008-06-30 CN CN201610865534.XA patent/CN106978393A/zh active Pending
- 2008-06-30 WO PCT/US2008/008120 patent/WO2009005770A1/en active Application Filing
- 2008-06-30 EP EP08779877.3A patent/EP2173864B1/en active Active
- 2008-06-30 JP JP2010514846A patent/JP5737936B2/ja active Active
- 2008-06-30 DK DK08779877.3T patent/DK2173864T3/da active
- 2008-06-30 US US12/452,281 patent/US10214720B2/en active Active
- 2008-06-30 CN CN200880102208.7A patent/CN101778935B/zh not_active Expired - Fee Related
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2010
- 2010-10-12 HK HK10109630.5A patent/HK1143604A1/zh not_active IP Right Cessation
- 2010-10-12 HK HK16108073.5A patent/HK1219984A1/zh unknown
-
2014
- 2014-10-31 JP JP2014222238A patent/JP6251152B2/ja active Active
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2017
- 2017-08-23 JP JP2017159904A patent/JP2018019703A/ja active Pending
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2019
- 2019-01-25 US US16/258,269 patent/US20190225935A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2015042184A (ja) | 2015-03-05 |
| EP2173864A4 (en) | 2011-09-14 |
| US20110177593A1 (en) | 2011-07-21 |
| EP3034606A1 (en) | 2016-06-22 |
| JP2010532167A (ja) | 2010-10-07 |
| CN101778935B (zh) | 2016-10-26 |
| EP2173864A1 (en) | 2010-04-14 |
| EP2173864B1 (en) | 2016-05-11 |
| US20190225935A1 (en) | 2019-07-25 |
| CA2691790C (en) | 2017-05-02 |
| WO2009005770A1 (en) | 2009-01-08 |
| US10214720B2 (en) | 2019-02-26 |
| HK1219984A1 (zh) | 2017-04-21 |
| JP2018019703A (ja) | 2018-02-08 |
| CN106978393A (zh) | 2017-07-25 |
| JP5737936B2 (ja) | 2015-06-17 |
| JP6251152B2 (ja) | 2017-12-20 |
| CA2691790A1 (en) | 2009-01-08 |
| HK1143604A1 (zh) | 2011-01-07 |
| CN101778935A (zh) | 2010-07-14 |
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