DK2147107T3 - Fremgangsmåde til ekspressionskloning, som er egnet til selektion af bibliotekskloner, der producerer et polypeptid af interesse - Google Patents

Fremgangsmåde til ekspressionskloning, som er egnet til selektion af bibliotekskloner, der producerer et polypeptid af interesse

Info

Publication number
DK2147107T3
DK2147107T3 DK08750156.5T DK08750156T DK2147107T3 DK 2147107 T3 DK2147107 T3 DK 2147107T3 DK 08750156 T DK08750156 T DK 08750156T DK 2147107 T3 DK2147107 T3 DK 2147107T3
Authority
DK
Denmark
Prior art keywords
library
interest
polynucleotide
polypeptide
host cell
Prior art date
Application number
DK08750156.5T
Other languages
English (en)
Inventor
Jesper Vind
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK2147107T3 publication Critical patent/DK2147107T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
DK08750156.5T 2007-05-09 2008-05-07 Fremgangsmåde til ekspressionskloning, som er egnet til selektion af bibliotekskloner, der producerer et polypeptid af interesse DK2147107T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200700694 2007-05-09
PCT/EP2008/055638 WO2008138835A1 (en) 2007-05-09 2008-05-07 Expression cloning method suitable for selecting library clones producing a polypeptide of interest

Publications (1)

Publication Number Publication Date
DK2147107T3 true DK2147107T3 (da) 2011-10-24

Family

ID=38226473

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08750156.5T DK2147107T3 (da) 2007-05-09 2008-05-07 Fremgangsmåde til ekspressionskloning, som er egnet til selektion af bibliotekskloner, der producerer et polypeptid af interesse

Country Status (7)

Country Link
US (1) US20100221783A1 (da)
EP (1) EP2147107B1 (da)
JP (1) JP5497632B2 (da)
CN (1) CN101679993A (da)
AT (1) ATE517188T1 (da)
DK (1) DK2147107T3 (da)
WO (1) WO2008138835A1 (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2010275672A1 (en) 2009-07-22 2012-02-02 Dsm Ip Assets B.V. Improved host cell for the production of a compound of interest
DK2683732T3 (da) * 2011-03-11 2016-12-12 Dsm Ip Assets Bv Vektor-vært-system
EP3597736A1 (en) 2011-11-21 2020-01-22 Novozymes A/S Gh61 polypeptide variants and polynucleotides encoding same
EP2841567B1 (en) 2012-04-27 2017-07-26 Novozymes, Inc. Gh61 polypeptide variants and polynucleotides encoding same
WO2014086706A1 (en) * 2012-12-07 2014-06-12 Novozymes A/S Method for generating site-specific mutations in filamentous fungi
WO2015059133A1 (en) 2013-10-22 2015-04-30 Novozymes A/S Cellobiose dehydrogenase variants and polynucleotides encoding same
CN105992821A (zh) * 2013-12-03 2016-10-05 诺维信公司 真菌基因文库的双分裂标记物整合
EP3114276B1 (en) 2014-03-05 2019-01-16 Novozymes A/S Compositions and methods for functionalizing and linking materials
EP3113609B1 (en) 2014-03-05 2019-07-31 Novozymes A/S Methods for improving post-harvest properties of agricultural crops
WO2015134737A1 (en) 2014-03-05 2015-09-11 Novozymes A/S Compositions and methods for improving properties of cellulosic textile materials with xyloglucan endotransglycosylase
US20170027167A1 (en) 2014-03-05 2017-02-02 Novozymes Bioag A/S Formulations comprising polymeric xyloglucan as a carrier for agriculturally beneficial agents
CN106062270A (zh) 2014-03-05 2016-10-26 诺维信公司 使用木葡聚糖内糖基转移酶改进非纤维素纺织材料的性质的组合物和方法
JP6725513B2 (ja) 2014-12-16 2020-07-22 ダニスコ・ユーエス・インク ヘルパー株媒介型真菌ゲノム改変用の組成物および方法
CN104894165B (zh) * 2015-05-26 2019-01-25 中国科学院青岛生物能源与过程研究所 一种提高基因打靶技术在土曲霉中应用效率的方法与应用
CN104894115B (zh) * 2015-05-26 2018-11-13 中国科学院青岛生物能源与过程研究所 一种具有高效同源重组能力的土曲霉及其构建方法与应用
WO2017070219A1 (en) 2015-10-20 2017-04-27 Novozymes A/S Lytic polysaccharide monooxygenase (lpmo) variants and polynucleotides encoding same
WO2018050666A1 (en) 2016-09-15 2018-03-22 Novozymes A/S Genomic integration of dna fragments in fungal host cells
JP7181865B2 (ja) * 2017-06-23 2022-12-01 株式会社カネカ メタノール資化性酵母内において高効率にベクターをアセンブルする方法
WO2019227363A1 (zh) * 2018-05-31 2019-12-05 大连民族大学 一种地生弯颈霉发酵生产脂肪酶的培养基及其培养方法
WO2020173817A1 (en) 2019-02-28 2020-09-03 Novozymes A/S Calcite binding proteins
CN112301047A (zh) * 2020-11-02 2021-02-02 江苏东玄基因科技有限公司 一种精确调控重组蛋白表达的方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
DE3886221T3 (de) 1987-09-04 2000-12-21 Novo Nordisk A/S, Bagsvaerd VERFAHREN ZUR HERSTELLUNG VON PROTEINPRODUKTEN IN -i(ASPERGILLUS) UND PROMOTOREN ZUR VERWENDUNG IN -i(ASPERGILLUS).
CA1341226C (en) 1988-08-16 2001-05-01 Wim Van Hartingsveldt Gene replacement as a tool for the construction of aspergillus strains
US5648267A (en) 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
CN1192108C (zh) 1994-06-03 2005-03-09 诺沃奇梅兹生物技术有限公司 纯化的毁丝霉属漆酶及编码该酶的核酸
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
CN1231588C (zh) 1996-01-19 2005-12-14 诺沃奇梅兹生物技术有限公司 丝状真菌的形态突变体
JP4198753B2 (ja) 1996-07-05 2008-12-17 ノボザイムス アクティーゼルスカブ 転写因子
WO2000024883A1 (en) * 1998-10-26 2000-05-04 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
EP1266016A2 (en) 2000-01-13 2002-12-18 Novozymes Biotech, Inc. Methods for producing a polypeptide using a crippled translational initiator sequence
US6569681B1 (en) * 2000-03-14 2003-05-27 Transkaryotic Therapies, Inc. Methods of improving homologous recombination
CN102174535B (zh) 2000-03-14 2014-04-09 诺维信公司 在制备多肽的方法中有效的真菌转录激活因子
EP1217074A1 (en) * 2000-12-22 2002-06-26 Universiteit Leiden Nucleic acid integration in eukaryotes
US7063962B2 (en) * 2001-07-20 2006-06-20 Novozymes A/S DNA sequences for regulating transcription
EP1478755B1 (en) 2002-02-19 2009-05-27 Novozymes A/S Expression cloning methods in filamentous fungi
US9243043B2 (en) * 2004-04-02 2016-01-26 Dsm Ip Assets B.V. Filamentous fungal mutants with improved homologous recombination efficiency

Also Published As

Publication number Publication date
EP2147107B1 (en) 2011-07-20
JP2010525830A (ja) 2010-07-29
JP5497632B2 (ja) 2014-05-21
ATE517188T1 (de) 2011-08-15
US20100221783A1 (en) 2010-09-02
WO2008138835A1 (en) 2008-11-20
EP2147107A1 (en) 2010-01-27
CN101679993A (zh) 2010-03-24

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