MX349596B - Metodo para la expresion de polipeptidos usando acidos nucleicos modificados. - Google Patents
Metodo para la expresion de polipeptidos usando acidos nucleicos modificados.Info
- Publication number
- MX349596B MX349596B MX2014011805A MX2014011805A MX349596B MX 349596 B MX349596 B MX 349596B MX 2014011805 A MX2014011805 A MX 2014011805A MX 2014011805 A MX2014011805 A MX 2014011805A MX 349596 B MX349596 B MX 349596B
- Authority
- MX
- Mexico
- Prior art keywords
- group
- polypeptide
- codons
- frequency
- codon
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente invención se refiere a un método para producir recombinantemente un polipéptido en una célula bacteriana caracterizado porque comprende la etapa de cultivar una célula bacteriana la cual comprende un ácido nucleico que codifica para el polipéptido, y recuperar el polipéptido de la célula bacteriana o del medio de cultivo, en donde cada uno de los residuos aminoácidos del polipéptido es codificado por el menos un codón , por lo que uno o varios de los codones que codifican para el mismo residuo aminoácido se combinan en un grupo y cada uno de los codones en un grupo se define por una frecuencia de uso específica dentro del grupo, la cual es la frecuencia con la cual un codón único de un grupo de codones puede ser encontrado en un ácido nucleico que codifica para un polipéptido en relación a todos los codones de un grupo, por lo que la suma de las frecuencias de uso específicas de todos los codones en un grupo es 100%, en donde la frecuencia de uso general de cada codón en el genoma de la célula es aproximadamente el mismo que su frecuencia de uso específico dentro de su grupo.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12164430 | 2012-04-17 | ||
| PCT/EP2013/057808 WO2013156443A1 (en) | 2012-04-17 | 2013-04-15 | Method for the expression of polypeptides using modified nucleic acids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2014011805A MX2014011805A (es) | 2014-12-08 |
| MX349596B true MX349596B (es) | 2017-08-04 |
Family
ID=48184161
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2014011805A MX349596B (es) | 2012-04-17 | 2013-04-15 | Metodo para la expresion de polipeptidos usando acidos nucleicos modificados. |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US20150246961A1 (es) |
| EP (2) | EP2839011B1 (es) |
| JP (2) | JP6224077B2 (es) |
| KR (1) | KR102064025B1 (es) |
| CN (2) | CN104245937B (es) |
| BR (1) | BR112014025693B1 (es) |
| CA (1) | CA2865676C (es) |
| ES (1) | ES2599386T3 (es) |
| MX (1) | MX349596B (es) |
| RU (1) | RU2014144881A (es) |
| WO (1) | WO2013156443A1 (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112014025693B1 (pt) * | 2012-04-17 | 2021-12-07 | F. Hoffmann-La Roche Ag | Método de produção recombinante de um polipeptídeo em uma célula bacteriana |
| WO2015017256A1 (en) | 2013-07-29 | 2015-02-05 | Danisco Us Inc. | Variant enzymes |
| US11345907B2 (en) * | 2018-05-29 | 2022-05-31 | Firmenich Sa | Method for producing albicanol compounds |
| GB201813528D0 (en) | 2018-08-20 | 2018-10-03 | Ucl Business Plc | Factor IX encoding nucleotides |
| CA3109579A1 (en) * | 2018-08-20 | 2020-02-27 | Ucl Business Ltd | Factor ix encoding nucleotides |
| BR112021025806A2 (pt) | 2019-06-28 | 2022-02-08 | Hoffmann La Roche | Métodos para produzir um anticorpo da subclasse igg1 humana cultivando uma célula cho e uso da remoção de sítios de splice de doador não pareados em uma parte de uma sequência de ácido nucleico de humano ou hamster |
| CN114774391B (zh) * | 2022-03-09 | 2023-03-14 | 华南农业大学 | 一种抗大肠杆菌的噬菌体内溶素及其应用 |
| CN114507682A (zh) * | 2022-03-16 | 2022-05-17 | 华南农业大学 | 编码重组NlpC/P60内肽酶蛋白的基因及其应用 |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4816567A (en) | 1983-04-08 | 1989-03-28 | Genentech, Inc. | Recombinant immunoglobin preparations |
| US5010182A (en) | 1987-07-28 | 1991-04-23 | Chiron Corporation | DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides |
| AU4005289A (en) | 1988-08-25 | 1990-03-01 | Smithkline Beecham Corporation | Recombinant saccharomyces |
| US5082767A (en) | 1989-02-27 | 1992-01-21 | Hatfield G Wesley | Codon pair utilization |
| US5959177A (en) | 1989-10-27 | 1999-09-28 | The Scripps Research Institute | Transgenic plants expressing assembled secretory antibodies |
| KR100249937B1 (ko) | 1991-04-25 | 2000-04-01 | 나가야마 오사무 | 인간 인터루킨-6 수용체에 대한 재구성 인간 항체 |
| WO1993006217A1 (en) | 1991-09-19 | 1993-04-01 | Genentech, Inc. | EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES |
| US5795737A (en) | 1994-09-19 | 1998-08-18 | The General Hospital Corporation | High level expression of proteins |
| US5789199A (en) | 1994-11-03 | 1998-08-04 | Genentech, Inc. | Process for bacterial production of polypeptides |
| US5840523A (en) | 1995-03-01 | 1998-11-24 | Genetech, Inc. | Methods and compositions for secretion of heterologous polypeptides |
| US6040498A (en) | 1998-08-11 | 2000-03-21 | North Caroline State University | Genetically engineered duckweed |
| EP0972838B1 (en) | 1998-07-15 | 2004-09-15 | Roche Diagnostics GmbH | Escherichia coli host/vector system based on antibiotic-free selection by complementation of an auxotrophy |
| US6291245B1 (en) | 1998-07-15 | 2001-09-18 | Roche Diagnostics Gmbh | Host-vector system |
| US7125978B1 (en) | 1999-10-04 | 2006-10-24 | Medicago Inc. | Promoter for regulating expression of foreign genes |
| NZ517906A (en) | 1999-10-04 | 2003-01-31 | Medicago Inc | Cloning of genomic sequences encoding nitrite reductase (NiR) for use in regulated expression of foreign genes in host plants |
| EP1156112B1 (en) | 2000-05-18 | 2006-03-01 | Geneart GmbH | Synthetic gagpol genes and their uses |
| GB0014288D0 (en) * | 2000-06-10 | 2000-08-02 | Smithkline Beecham Biolog | Vaccine |
| DE10037111A1 (de) * | 2000-07-27 | 2002-02-07 | Boehringer Ingelheim Int | Herstellung eines rekombinanten Proteins in einer prokaryontischen Wirtszelle |
| JP2004535166A (ja) * | 2001-03-22 | 2004-11-25 | デンドレオン・サンディエゴ・リミテッド・ライアビリティ・カンパニー | セリンプロテアーゼｃｖｓｐ１４をコードする核酸分子、コードされるポリペプチドおよびそれに基づく方法 |
| AU2003206684A1 (en) | 2002-02-20 | 2003-09-09 | Novozymes A/S | Plant polypeptide production |
| CA2480504A1 (en) | 2002-04-01 | 2003-10-16 | Evelina Angov | Method of designing synthetic nucleic acid sequences for optimal protein expression in a host cell |
| WO2003093296A2 (en) * | 2002-05-03 | 2003-11-13 | Sequenom, Inc. | Kinase anchor protein muteins, peptides thereof, and related methods |
| CA2443365C (en) | 2002-11-19 | 2010-01-12 | F. Hoffmann-La Roche Ag | Methods for the recombinant production of antifusogenic peptides |
| GB0308988D0 (en) * | 2003-04-17 | 2003-05-28 | Univ Singapore | Molecule |
| JP4228072B2 (ja) * | 2003-06-04 | 2009-02-25 | 独立行政法人農業・食品産業技術総合研究機構 | アビジンをコードする人工合成遺伝子 |
| US20060024670A1 (en) | 2004-05-18 | 2006-02-02 | Luke Catherine J | Influenza virus vaccine composition and methods of use |
| AU2007254993A1 (en) | 2006-05-30 | 2007-12-13 | Dow Global Technologies Llc | Codon optimization method |
| US7884262B2 (en) | 2006-06-06 | 2011-02-08 | Monsanto Technology Llc | Modified DMO enzyme and methods of its use |
| CN101627053A (zh) * | 2006-06-12 | 2010-01-13 | 西福根有限公司 | 泛细胞表面受体特异的治疗剂 |
| KR20090031897A (ko) | 2006-06-12 | 2009-03-30 | 리셉터 바이오로직스 인크 | 전-세포 표면 수용체-특이적 치료제 |
| EP2035561A1 (en) | 2006-06-29 | 2009-03-18 | DSMIP Assets B.V. | A method for achieving improved polypeptide expression |
| BR112014025693B1 (pt) | 2012-04-17 | 2021-12-07 | F. Hoffmann-La Roche Ag | Método de produção recombinante de um polipeptídeo em uma célula bacteriana |
-
2013
- 2013-04-15 BR BR112014025693-4A patent/BR112014025693B1/pt active IP Right Grant
- 2013-04-15 MX MX2014011805A patent/MX349596B/es active IP Right Grant
- 2013-04-15 EP EP13718536.9A patent/EP2839011B1/en active Active
- 2013-04-15 KR KR1020147028712A patent/KR102064025B1/ko active Active
- 2013-04-15 CN CN201380020266.6A patent/CN104245937B/zh active Active
- 2013-04-15 CN CN202111149491.2A patent/CN114107352A/zh active Pending
- 2013-04-15 WO PCT/EP2013/057808 patent/WO2013156443A1/en not_active Ceased
- 2013-04-15 EP EP16188552.0A patent/EP3138917B2/en active Active
- 2013-04-15 JP JP2015506201A patent/JP6224077B2/ja active Active
- 2013-04-15 RU RU2014144881A patent/RU2014144881A/ru not_active Application Discontinuation
- 2013-04-15 CA CA2865676A patent/CA2865676C/en active Active
- 2013-04-15 ES ES13718536.9T patent/ES2599386T3/es active Active
-
2014
- 2014-10-17 US US14/517,516 patent/US20150246961A1/en not_active Abandoned
-
2017
- 2017-10-04 JP JP2017193965A patent/JP2018038408A/ja active Pending
- 2017-12-06 US US15/833,012 patent/US12577293B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3138917A1 (en) | 2017-03-08 |
| CA2865676C (en) | 2020-03-10 |
| JP2015514406A (ja) | 2015-05-21 |
| CA2865676A1 (en) | 2013-10-24 |
| US20150246961A1 (en) | 2015-09-03 |
| CN114107352A (zh) | 2022-03-01 |
| US20180100006A1 (en) | 2018-04-12 |
| EP2839011B1 (en) | 2016-09-14 |
| KR20140146105A (ko) | 2014-12-24 |
| HK1205186A1 (en) | 2015-12-11 |
| ES2599386T3 (es) | 2017-02-01 |
| MX2014011805A (es) | 2014-12-08 |
| EP3138917B1 (en) | 2019-08-21 |
| BR112014025693B1 (pt) | 2021-12-07 |
| JP6224077B2 (ja) | 2017-11-01 |
| BR112014025693A2 (en) | 2018-08-07 |
| KR102064025B1 (ko) | 2020-01-08 |
| EP3138917B2 (en) | 2024-10-23 |
| EP2839011A1 (en) | 2015-02-25 |
| RU2014144881A (ru) | 2016-06-10 |
| US12577293B2 (en) | 2026-03-17 |
| CN104245937B (zh) | 2021-09-21 |
| CN104245937A (zh) | 2014-12-24 |
| WO2013156443A1 (en) | 2013-10-24 |
| JP2018038408A (ja) | 2018-03-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |