DK2129777T3 - Multizymer og anvendelse heraf til fremstilling af polyumættede fedtsyrer - Google Patents

Multizymer og anvendelse heraf til fremstilling af polyumættede fedtsyrer Download PDF

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DK2129777T3
DK2129777T3 DK08727277.9T DK08727277T DK2129777T3 DK 2129777 T3 DK2129777 T3 DK 2129777T3 DK 08727277 T DK08727277 T DK 08727277T DK 2129777 T3 DK2129777 T3 DK 2129777T3
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seq
delta
desaturase
elongase
plant
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DK08727277.9T
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Howard G Damude
Anthony J Kinney
Kevin G Ripp
Quinn Qun Zhu
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Du Pont
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19001—Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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Claims (23)

  1. MULTIZYMER OG ANVENDELSE HERAF TIL FREMSTILLING AF POLYUMÆTTEDE FEDTSYRER
    1. Multizym, der omfatter et enkelt polypeptid, og som har mindst to uafhængige og adskillelige enzymatiske aktiviteter, hvor de enzymatiske aktiviteter omfatter mindst én fedtsyreelongase, der er forbundet med mindst én fedtsyredesaturase, hvor elongasen er forbundet med desaturasen og linket er udvalgt fra gruppen bestående af en SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 235, SEQ ID NO: 438, SEQ ID NO: 472, SEQ ID NO: 445 og SEQ ID NO: 504.
  2. 2. Multizym ifølge krav 1, hvor fedtsyreelongasen er en C2o/22-elongase og fedtsyredesaturasen er en delta-4-desaturase.
  3. 3. Multizym ifølge krav 1, hvor fedtsyreelongasen er en delta-9-elongase og fedtsyredesaturasen er en delta-8-desaturase.
  4. 4. Isoleret polynukleotid, der koder for et multizym ifølge krav 1, hvor polynukleotidet omfatter: (a) en nukleotidsekvens, der koder for et polypeptid, der har DHA-syntaseaktivitet, hvor polypeptidet er mindst 80 % aminosyreidentisk, baseret på Clustal V-alignmentsmetoden, ved sammenligning med en aminosyresekvens som vist i SEQ ID NO: 12, SEQ ID NO: 22, SEQ ID NO: 95, SEQ ID NO: 96 eller SEQ ID NO: 97; (b) en nukleotidsekvens, der koder for et polypeptid, der har DHA-syntaseaktivitet, hvor nukleotidsekvensen er mindst 80 % sekvensidentisk, baseret på BLASTN-alignmentsmetoden, ved sammenligning med en nukleotidsekvens som vist i SEQ ID NO: 11, SEQ ID NO: 205, SEQ ID NO: 21, SEQ ID NO: 91, SEQ ID NO: 92, SEQ ID NO: 93 eller SEQ ID NO: 410; (c) en nukleotidsekvens, der koder for et polypeptid, der har DHA-syntaseaktivitet, hvor nukleotidsekvensen hybridiser under stringente forhold til en nukleotidsekvens som vist i SEQ ID NO: 11, SEQ ID NO: 205, SEQ ID NO: 21, SEQ ID NO: 91, SEQ ID NO: 92, SEQ ID NO: 93 eller SEQ ID NO: 410; eller (d) et komplement af nukleotidsekvensen af (a), (b) eller (c), hvor komplementet og nukleotidsekvensen består af samme antal nukleotider og er 100 % komplementære.
  5. 5. Isoleret polynukleotid ifølge krav 4, hvor polynukleotidet koder for et polypeptid, der har en aminosyresekvens som vist i SEQ ID NO: 12, SEQ ID NO: 22, SEQ ID NO: 95, SEQ ID NO: 96 eller SEQ ID NO: 97.
  6. 6. Isoleret polynukleotid ifølge krav 5, hvor polynukleotidet omfatter en nukleotidsekvens som vist i SEQ ID NO: 11, SEQ ID NO: 21, SEQ ID NO: 91, SEQ ID NO: 92, SEQ ID NO: 93, SEQ ID NO: 205 eller SEQ ID NO: 410.
  7. 7. Isoleret polynukleotid, der koder for et multizym ifølge krav 1, hvor polynukleotidet omfatter: (a) en nukleotidsekvens, der koder for et polypeptid, der har DGLA-syntaseaktivitet, hvor polypeptidet er vist i SEQ ID NO: 441, SEQ ID NO: 447, SEQ ID NO: 454, SEQ ID NO: 461, SEQ ID NO: 464, SEQ ID NO: 471, SEQ ID NO: 515, SEQ ID NO: 516, SEQ ID NO: 517, SEQ ID NO: 518 eller SEQ ID NO: 519; (b) en nukleotidsekvens, der koder for et polypeptid, der har DGLA-syntaseaktivitet, hvor nukleotidsekvensen er vist i SEQ ID NO: 440, SEQ ID NO: 446, SEQ ID NO: 453, SEQ ID NO: 460, SEQ ID NO: 463, SEQ ID NO: 470, SEQ ID NO: 492, SEQ ID NO: 493, SEQ ID NO: 494, SEQ ID NO: 495 eller SEQ ID NO: 496; (c) en nukleotidsekvens, der koder for et polypeptid, der har DGLA-syntaseaktivitet, hvor nukleotidsekvensen hybridiserer under stringente forhold til en nukleotidsekvens som vist i SEQ ID NO: 440, SEQ ID NO; 446, SEQ ID NO: 453, SEQ ID NO; 460, SEQ ID NO: 463, SEQ ID NO: 470, SEQ ID NO: 492, SEQ ID NO: 493, SEQ ID NO: 494, SEQ ID NO: 495 eller SEQ ID NO: 496; eller (d) et komplement af nukleotidsekvensen af (a), (b) eller (c), hvor komplementet og nukleotidsekvensen består af det samme antal nukleotider og er 100 % komplementære.
  8. 8. Rekombinant konstruktion, der omfatter et hvilket som helst af de isolerede polynukleotider ifølge kravene 4 til 7 operativt forbundet med mindst én regulatorisk sekvens.
  9. 9. Værtscelle, der i sit genom omfatter den rekombinante konstruktion ifølge krav 8.
  10. 10. Værtscelle ifølge krav 9, hvor værtscellen er en plantecelle.
  11. 11. Værtscelle ifølge krav 10, hvor plantecellen er en plantecelle fra et olieholdigt frø.
  12. 12. Værtscelle ifølge krav 9, hvor værtscellen er en gærcelle.
  13. 13. Værtscelle ifølge krav 12, hvor gærcellen er en olieholdig gærcelle.
  14. 14. Værtscelle ifølge krav 13, hvor den olieholdige gærcelle er en Yarrowia sp.
  15. 15. Fremgangsmåde til transformering af en celle, hvilken fremgangsmåde omfatter transformering af en celle med den rekombinante konstruktion ifølge krav 8 og udvælgelse af de celler, der er transformeret med den rekombinante konstruktion.
  16. 16. Fremgangsmåde til fremstilling af en transformeret plante, hvilken fremgangsmåde omfatter transformering af en plantecelle med den rekombinante konstruktion ifølge krav 8 og regenerering af en plante fra den transformerede plantecelle.
  17. 17. Fremgangsmåde ifølge krav 16, hvor planten er en sojabønneplante.
  18. 18. Plante, der i sit genom omfatter den rekombinante konstruktion ifølge krav 8.
  19. 19. Plante ifølge krav 18, hvor planten er en olieplante.
  20. 20. Plante ifølge krav 19, hvor olieplanten er sojabønne.
  21. 21. Frø opnået fra planten ifølge et hvilket som helst af kravene 18 til 20, hvor frøet omfatter den rekombinante konstruktion ifølge krav 8.
  22. 22. Fremgangsmåde til omdannelse af linolsyre til dihomo-gammalinolsyre eller α-linolsyre til eicosatetraensyre, der omfatter: a) tilvejebringelse af en mikrobiel værtscelle, der omfatter den rekombinante konstruktion ifølge krav 8, og som koder for en DGLA-syntase og en linolsyrekilde eller en α-linolsyrekilde; og b) dyrkning af værtscellen fra (a) under forhold, hvorved dihomo-gamma-linolsyren eller eicosatetraensyre fremstilles.
  23. 23. Fremgangsmåde til omdannelse af eicosapentaensyre to docosahexaensyre, der omfatter: a) tilvejebringelse af en mikrobiel værtscelle, der omfatter den rekombinante konstruktion ifølge krav 8, og som koder for en DHA-syntase og en kilde af eicosapentaensyre; og b) dyrkning af værtscellen under (a) under forhold, hvorved der produceres docosahexaensyre.
DK08727277.9T 2007-04-03 2008-04-03 Multizymer og anvendelse heraf til fremstilling af polyumættede fedtsyrer DK2129777T3 (da)

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US90979007P 2007-04-03 2007-04-03
US2789808P 2008-02-12 2008-02-12
PCT/US2008/004377 WO2008124048A2 (en) 2007-04-03 2008-04-03 Multizymes and their use in making polyunsaturated fatty acids

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US (2) US8828690B2 (da)
EP (1) EP2129777B1 (da)
JP (1) JP2010523113A (da)
CN (2) CN101765658B (da)
BR (1) BRPI0808577A2 (da)
CA (1) CA2679988A1 (da)
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ES (1) ES2559312T3 (da)
HU (1) HUE028692T2 (da)
MX (1) MX2009010574A (da)
RS (1) RS20090413A (da)
RU (2) RU2517608C2 (da)
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2884237C (en) 2004-04-22 2020-09-08 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
ES2529572T3 (es) 2004-04-22 2015-02-23 Commonwealth Scientific And Industrial Research Organisation Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes
WO2008025068A1 (en) 2006-08-29 2008-03-06 Commonwealth Scientific And Industrial Research Organisation Synthesis of fatty acids
US7794701B2 (en) * 2007-04-16 2010-09-14 E.I. Du Pont De Nemours And Company Δ-9 elongases and their use in making polyunsaturated fatty acids
US7943365B2 (en) * 2007-05-03 2011-05-17 E.I. Du Pont De Nemours And Company Δ-5 desaturases and their use in making polyunsaturated fatty acids
US8318914B2 (en) * 2007-07-31 2012-11-27 Bioriginal Food & Science Corp. Elongases and methods for producing polyunsaturated fatty acids in transgenic organisms
US8323935B2 (en) * 2007-10-03 2012-12-04 E I Du Pont De Nemours And Company Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
US20110008868A1 (en) * 2008-04-03 2011-01-13 E.I. Du Pont De Nemours And Company Multizymes
US8168858B2 (en) 2008-06-20 2012-05-01 E. I. Du Pont De Nemours And Company Delta-9 fatty acid elongase genes and their use in making polyunsaturated fatty acids
CA2743880C (en) 2008-11-18 2020-08-18 James Robertson Petrie Recombinant cells comprising exogenous a5 elongase and methods for producing docosapentaenoic acid
CN102257126B (zh) 2008-12-18 2014-07-09 纳幕尔杜邦公司 减少发酵过程中的丙二酸副产物
US8637298B2 (en) * 2009-06-16 2014-01-28 E I Du Pont De Nemours And Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
JP2012530182A (ja) * 2009-06-16 2012-11-29 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 改良されたヤロウイア・リポリティカ（ｙａｒｒｏｗｉａｌｉｐｏｌｙｔｉｃａ）最適化株による高エイコサペンタエン酸油
CA2763424C (en) 2009-06-16 2018-06-12 E.I. Du Pont De Nemours And Company Improvement of long chain omega-3 and omega-6 polyunsaturated fatty acid biosynthesis by expression of acyl-coa lysophospholipid acyltransferases
CN102803289B (zh) * 2009-06-16 2015-07-22 纳幕尔杜邦公司 用于高水平生产二十碳五烯酸的改善的优化的解脂耶氏酵母菌株
EP2449099A2 (en) 2009-06-30 2012-05-09 E. I. du Pont de Nemours and Company Plant seeds with altered storage compound levels, related constructs and methods involving genes encoding cytosolic pyrophosphatase
US8188335B2 (en) 2009-07-17 2012-05-29 Abbott Laboratories Δ9-elongase for production of polyunsaturated fatty acid-enriched oils
WO2011006948A1 (en) 2009-07-17 2011-01-20 Basf Plant Science Company Gmbh Novel fatty acid desaturases and elongases and uses thereof
AR078829A1 (es) 2009-10-30 2011-12-07 Du Pont Plantas y semillas con niveles alterados de compuesto de almacenamiento, construcciones relacionadas y metodos relacionados con genes que codifican proteinas similares a las aldolasas bacterianas de la clase ii del acido 2,4- dihidroxi-hept-2-eno-1,7-dioico
CN102665431A (zh) 2009-11-03 2012-09-12 帝斯曼知识产权资产管理有限公司 包含细胞和具有至少20个碳原子的多不饱和脂肪酸(lc-pufa)的组合物
CA2779552C (en) 2009-11-03 2019-04-09 Dsm Ip Assets B.V. Vegetable oil comprising a polyunsaturated fatty acid having at least 20 carbon atoms
EP2504441B1 (en) 2009-11-23 2020-07-22 E. I. du Pont de Nemours and Company Sucrose transporter genes for increasing plant seed lipids
AU2010333827A1 (en) 2009-12-24 2012-06-28 E. I. Du Pont De Nemours And Company Plant membrane bound O-acyl transferase (MBOAT) family protein sequences and their uses for altering fatty acid compositions
WO2011087981A2 (en) 2010-01-15 2011-07-21 E. I. Du Pont De Nemours And Company Clinical benefits of eicosapentaenoic acid in humans
AU2011223619B2 (en) * 2010-03-02 2016-01-21 Massachusetts Institute Of Technology Microbial engineering for the production of fatty acids and fatty acid derivatives
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US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
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US20120247066A1 (en) 2011-04-01 2012-10-04 Ice House America, Llc Ice bagging apparatus and methods
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WO2013112523A2 (en) * 2012-01-23 2013-08-01 E. I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial micrornas for silencing fatty acid biosynthestic genes
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CA2877279A1 (en) 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company Improved production of polyunsaturated fatty acids by coexpression of acyl-coa:lysophosphatidylcholine acyltransferases and phospholipid:diacylglycerol acyltransferases
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PE20170253A1 (es) 2013-12-18 2017-04-14 Nuseed Pty Ltd Lipido que comprende acidos grasos poliinsaturados de cadena larga
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US10393225B2 (en) * 2015-01-05 2019-08-27 Goodrich Corporation Integrated multi-function propulsion belt for air cushion supported aircraft cargo loading robot
EA201991467A1 (ru) 2016-12-15 2019-11-29 Состав смеси, содержащий силикат и микробные и/или растительные клетки и полиненасыщенную жирную кислоту, имеющую по меньшей мере 20 атомов углерода (lc-pufa)
WO2018129440A1 (en) 2017-01-09 2018-07-12 University Of Massachusetts Complexes for gene deletion and editing
CN115820586A (zh) * 2022-09-06 2023-03-21 中国农业科学院油料作物研究所 一种溶血磷脂酰胆碱酰基转移酶、核酸分子及其应用

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07501701A (ja) 1991-12-04 1995-02-23 イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー 植物由来の脂肪酸不飽和化酵素遺伝子
US5547868A (en) * 1993-06-09 1996-08-20 Regents Of The University Of California Cholesterol disposal fusion enzymes
US5968809A (en) * 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US6075183A (en) * 1997-04-11 2000-06-13 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
US6677145B2 (en) 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
US6403349B1 (en) 1998-09-02 2002-06-11 Abbott Laboratories Elongase gene and uses thereof
US6825017B1 (en) * 1998-12-07 2004-11-30 Washington State University Research Foundation Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
DE69935162T2 (de) 1998-12-07 2007-11-22 Washington State University Research Foundation, Pullman Desaturasen und verfahren ihrer anwendung zur synthese von mehrfach ungesättigten fettsäuren
RU2336307C2 (ru) * 2000-01-19 2008-10-20 Мартек Биосайнсис Корпорейшн Способ получения липидов (варианты) и липиды, полученные этим способом
ATE510908T1 (de) * 2000-09-28 2011-06-15 Bioriginal Food & Science Corp Fad5-2, mitglied der desaturase-familie und verwendungen davon
DE10102337A1 (de) * 2001-01-19 2002-07-25 Basf Plant Science Gmbh Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren, neue Biosynthesegene sowie neue pflanzliche Expressionskonstrukte
DE10102338A1 (de) * 2001-01-19 2002-07-25 Basf Plant Science Gmbh Verfahren zur Expression von Biosynthesegenen in pflanzlichen Samen unter Verwendung von neuen multiplen Expressionskonstrukten
WO2002081668A2 (en) 2001-01-25 2002-10-17 Abbott Laboratories Desaturase genes and uses thereof
GB0107510D0 (en) 2001-03-26 2001-05-16 Univ Bristol New elongase gene and a process for the production of -9-polyunsaturated fatty acids
US7045683B2 (en) * 2001-05-04 2006-05-16 Abbott Laboratories Δ4-desaturase genes and uses thereof
BR0317304A (pt) * 2002-12-19 2005-11-08 Univ Bristol Processo para a produção de compostos, sequência de ácido nucleico isolada, sequência de aminoácidos, construção gênica, vetor, e, organismo
US20040172682A1 (en) 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
MXPA05010571A (es) * 2003-04-08 2005-11-23 Basf Plant Science Gmbh Delta-4-desaturasas a partir de euglena gracilis, que expresan plantas y aceites que comprenden pufa
US7214491B2 (en) * 2003-05-07 2007-05-08 E. I. Du Pont De Nemours And Company Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
US7125672B2 (en) * 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7238482B2 (en) * 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
EP1654344B1 (de) * 2003-08-01 2018-10-17 BASF Plant Science GmbH Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen
US7504259B2 (en) 2003-11-12 2009-03-17 E. I. Du Pont De Nemours And Company Δ12 desaturases suitable for altering levels of polyunsaturated fatty acids in oleaginous yeast
AU2004290051A1 (en) 2003-11-12 2005-05-26 E.I. Dupont De Nemours And Company Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oilseed plants and oleaginous yeast
MXPA06009572A (es) * 2004-02-27 2007-05-23 Basf Plant Science Gmbh Metodo para la produccion de acidos grasos poliinsaturados en plantas transgenicas.
ES2529572T3 (es) * 2004-04-22 2015-02-23 Commonwealth Scientific And Industrial Research Organisation Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes
CA2884237C (en) 2004-04-22 2020-09-08 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
WO2006012326A1 (en) 2004-06-25 2006-02-02 E.I. Dupont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7189559B2 (en) * 2004-11-04 2007-03-13 E. I. Du Pont De Nemours And Company Mortierella alpina lysophosphatidic acid acyltransferase homolog for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7470532B2 (en) 2005-10-19 2008-12-30 E.I. Du Pont De Nemours And Company Mortierella alpina C16/18 fatty acid elongase
AU2006318738B2 (en) 2005-11-23 2011-12-22 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids

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