DK3215611T3 - Peptid-medieret indgivelse af rna-guidet endonuklease i celler - Google Patents

Peptid-medieret indgivelse af rna-guidet endonuklease i celler Download PDF

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DK3215611T3
DK3215611T3 DK15813149T DK15813149T DK3215611T3 DK 3215611 T3 DK3215611 T3 DK 3215611T3 DK 15813149 T DK15813149 T DK 15813149T DK 15813149 T DK15813149 T DK 15813149T DK 3215611 T3 DK3215611 T3 DK 3215611T3
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rna
peptide
cells
guided endonuclease
mediated administration
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DK15813149T
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English (en)
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Ryan L Frisch
Xiaochun Fan
Seung-Pyo Hong
Ethel Noland Jackson
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Du Pont
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3513—Protein; Peptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK15813149T 2014-11-06 2015-11-03 Peptid-medieret indgivelse af rna-guidet endonuklease i celler DK3215611T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462075999P 2014-11-06 2014-11-06
PCT/US2015/058760 WO2016073433A1 (en) 2014-11-06 2015-11-03 Peptide-mediated delivery of rna-guided endonuclease into cells

Publications (1)

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DK3215611T3 true DK3215611T3 (da) 2019-11-25

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Application Number Title Priority Date Filing Date
DK19186202.8T DK3597740T3 (da) 2014-11-06 2015-11-03 Peptidmedieret indføring af rna-styret endonuklease i celler
DK15813149T DK3215611T3 (da) 2014-11-06 2015-11-03 Peptid-medieret indgivelse af rna-guidet endonuklease i celler

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US (3) US10208298B2 (da)
EP (2) EP3215611B1 (da)
JP (1) JP6823593B2 (da)
KR (1) KR102424721B1 (da)
CN (1) CN107406838A (da)
AU (1) AU2015343307B2 (da)
CA (1) CA2966731C (da)
DK (2) DK3597740T3 (da)
EA (1) EA038321B1 (da)
WO (1) WO2016073433A1 (da)

Families Citing this family (83)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2734621B1 (en) 2011-07-22 2019-09-04 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
WO2016182959A1 (en) 2015-05-11 2016-11-17 Editas Medicine, Inc. Optimized crispr/cas9 systems and methods for gene editing in stem cells
EP3307887A1 (en) 2015-06-09 2018-04-18 Editas Medicine, Inc. Crispr/cas-related methods and compositions for improving transplantation
JP7059179B2 (ja) * 2015-10-20 2022-04-25 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル 遺伝子操作のための方法及び製品
IL310721A (en) * 2015-10-23 2024-04-01 Harvard College Nucleobase editors and their uses
BR112018007796A2 (pt) 2015-11-06 2018-10-30 Du Pont plantas de soja, partes de plantas de soja ou sementes de soja, método para selecionar uma célula de soja, métodos de seleção de uma célula de soja e de produção de um locus e molécula de ácido nucleico
US20190211317A1 (en) * 2016-05-27 2019-07-11 Aadigen, Llc Peptides and nanoparticles for intracellular delivery of genome-editing molecules
CA3025523A1 (en) * 2016-05-27 2017-11-30 Aadigen, Llc Peptides and nanoparticles for intracellular delivery of genome-editing molecules
WO2017222834A1 (en) * 2016-06-10 2017-12-28 City Of Hope Compositions and methods for mitochondrial genome editing
AU2017286122A1 (en) 2016-06-14 2018-11-22 Pioneer Hi-Bred International, Inc. Use of Cpf1 endonuclease for plant genome modifications
US20190218257A1 (en) 2016-06-24 2019-07-18 The Scripps Research Institute Novel nucleoside triphosphate transporter and uses thereof
BR112019000430A2 (pt) * 2016-07-13 2019-07-09 Dsm Ip Assets Bv sistema crispr-cas para uma célula hospedeira de algas
CA3032699A1 (en) 2016-08-03 2018-02-08 President And Fellows Of Harvard College Adenosine nucleobase editors and uses thereof
AU2017308889B2 (en) 2016-08-09 2023-11-09 President And Fellows Of Harvard College Programmable Cas9-recombinase fusion proteins and uses thereof
US11352647B2 (en) 2016-08-17 2022-06-07 The Broad Institute, Inc. Crispr enzymes and systems
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US11319546B2 (en) 2016-09-28 2022-05-03 Cellivery Therapeutics, Inc. Cell-permeable (CP)-Cas9 recombinant protein and uses thereof
CA3037560A1 (en) 2016-10-13 2018-04-19 Pioneer Hi-Bred International, Inc. Generating northern leaf blight resistant maize
CN110214180A (zh) 2016-10-14 2019-09-06 哈佛大学的校长及成员们 核碱基编辑器的aav递送
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
WO2018129440A1 (en) * 2017-01-09 2018-07-12 University Of Massachusetts Complexes for gene deletion and editing
CN108384779A (zh) * 2017-02-03 2018-08-10 武汉臻智生物科技有限公司 微生物及其用途
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
CN110914426A (zh) 2017-03-23 2020-03-24 哈佛大学的校长及成员们 包含核酸可编程dna结合蛋白的核碱基编辑器
AU2018243654A1 (en) 2017-03-31 2019-10-17 Pioneer Hi-Bred International, Inc. Expression modulating elements and use thereof
WO2018204777A2 (en) 2017-05-05 2018-11-08 The Broad Institute, Inc. Methods for identification and modification of lncrna associated with target genotypes and phenotypes
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
KR102151064B1 (ko) * 2017-05-24 2020-09-02 기초과학연구원 매칭된 5' 뉴클레오타이드를 포함하는 가이드 rna를 포함하는 유전자 교정용 조성물 및 이를 이용한 유전자 교정 방법
CN108977442B (zh) * 2017-06-05 2023-01-06 广州市锐博生物科技有限公司 用于dna编辑的系统及其应用
KR102007457B1 (ko) * 2017-06-30 2019-08-05 동국대학교 산학협력단 Cas9 단백질, 가이드 RNA 및 양친매성 펩타이드를 포함하는 나노복합체 및 이의 용도
AU2018300069A1 (en) 2017-07-11 2020-02-27 Synthorx, Inc. Incorporation of unnatural nucleotides and methods thereof
EP3652320A4 (en) * 2017-07-12 2021-04-14 Mayo Foundation for Medical Education and Research MATERIALS AND PROCEDURES FOR EFFICIENT TARGETED KNOCK-IN OR GENERAL REPLACEMENT
US11866726B2 (en) 2017-07-14 2024-01-09 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
US11319532B2 (en) 2017-08-30 2022-05-03 President And Fellows Of Harvard College High efficiency base editors comprising Gam
AU2018352592A1 (en) 2017-10-16 2020-06-04 Beam Therapeutics, Inc. Uses of adenosine base editors
WO2019089452A1 (en) * 2017-10-30 2019-05-09 The Penn State Research Foundation Targeting peptide to deliver a compound to oocytes
EP3720271A1 (en) * 2017-12-04 2020-10-14 University of Florida Research Foundation Genome editing by pollen-mediated transformation
AU2018386002A1 (en) 2017-12-15 2020-05-28 Danisco Us Inc CAS9 variants and methods of use
WO2019138052A1 (en) * 2018-01-11 2019-07-18 Kws Saat Se Optimized plant crispr/cpf1 systems
WO2019173125A1 (en) 2018-03-09 2019-09-12 Pioneer Hi-Bred International, Inc. Compositions and methods for modification of fatty acids in soybean
JPWO2019189814A1 (ja) * 2018-03-30 2021-04-01 国立研究開発法人国立国際医療研究センター 細胞のダイレクトリプログラミング方法
WO2019217336A2 (en) * 2018-05-06 2019-11-14 Massachusetts Institute Of Technology APPLICATIONS OF STREPTOCOCCUS-DERIVED Cas9 NUCLEASSES ON MINIMAL ADENINE-RICH PAM TARGETS
CN112204156A (zh) 2018-05-25 2021-01-08 先锋国际良种公司 用于通过调节重组率来改善育种的系统和方法
CN113286620A (zh) * 2018-08-24 2021-08-20 莱兰斯坦福初级大学评议会 用于多核苷酸空间组织的系统和方法
CA3109984A1 (en) 2018-10-16 2020-04-23 Pioneer Hi-Bred International, Inc. Genome edited fine mapping and causal gene identification
CN113462672A (zh) * 2018-11-15 2021-10-01 中国农业大学 CRISPR-Cas12j酶和系统
KR20210104068A (ko) 2018-12-14 2021-08-24 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 게놈 편집을 위한 신규한 crispr-cas 시스템
CA3124395A1 (en) * 2018-12-20 2020-06-25 Basf Plant Science Company Gmbh Native delivery of biomolecules into plant cells using ionic complexes with cell-penetrating peptides
DE212020000516U1 (de) * 2019-03-07 2022-01-17 The Regents of the University of California CRISPR-CAS-Effektorpolypeptide
AU2020242032A1 (en) 2019-03-19 2021-10-07 Massachusetts Institute Of Technology Methods and compositions for editing nucleotide sequences
RU2707542C1 (ru) 2019-03-28 2019-11-27 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ получения препарата рекомбинантной нуклеазы CAS, по существу, свободного от бактериальных эндотоксинов, полученный данным способом препарат и содержащий его набор для использования в системе CRISPR/Cas
WO2020236967A1 (en) 2019-05-20 2020-11-26 The Broad Institute, Inc. Random crispr-cas deletion mutant
US11879145B2 (en) 2019-06-14 2024-01-23 The Scripps Research Institute Reagents and methods for replication, transcription, and translation in semi-synthetic organisms
KR102176415B1 (ko) * 2019-08-02 2020-11-09 중앙대학교 산학협력단 원핵세포 투과성 재조합 단백질 및 이의 용도
CN112442512A (zh) * 2019-08-30 2021-03-05 华中农业大学 基于tRNA-gRNA-cRNA进行日本青鳉胚胎和细胞的基因编辑系统
WO2021041922A1 (en) 2019-08-30 2021-03-04 The Broad Institute, Inc. Crispr-associated mu transposase systems
CN111235130B (zh) * 2019-11-15 2022-11-25 武汉大学 II类V型CRISPR蛋白CeCas12a及其在基因编辑的应用
CN112813049B (zh) * 2019-11-18 2023-02-17 中国科学院分子细胞科学卓越创新中心 用于活细胞rna标记的融合蛋白及应用
WO2021098709A1 (zh) * 2019-11-18 2021-05-27 中国科学院遗传与发育生物学研究所 衍生自黄杆菌的基因编辑系统
IL292896A (en) * 2019-12-23 2022-07-01 Univ California CRISPR-Cas execution poplipeptides and methods of using them
CN111378612B (zh) * 2020-03-10 2023-05-09 山东大学 一种利用重组胞内rna支架固定重组酶促进细胞催化阿洛醇生产的方法
JP2023517992A (ja) 2020-03-12 2023-04-27 インスティチュート フォー ベーシック サイエンス ゲノム配列変異を有する細胞死滅誘導用組成物及び該組成物を用いた細胞死滅誘導方法
CA3177481A1 (en) 2020-05-08 2021-11-11 David R. Liu Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
CA3175936A1 (en) 2020-06-12 2021-12-16 Pioneer Hi-Bred International, Inc. Alteration of seed composition in plants
CA3186862A1 (en) 2020-08-18 2022-02-24 Pioneer Hi-Bred International, Inc. Multiple disease resistance genes and genomic stacks thereof
CN112226425B (zh) * 2020-09-03 2022-09-30 宁夏中微泰克生物技术有限责任公司 一种亚洲小车蝗胞外蛋白酶erk及其编码基因与应用
CN115397980A (zh) * 2020-12-21 2022-11-25 国立大学法人鸟取大学 利用细胞膜穿透肽而不依赖于基因重组的植物基因组编辑技术
CN114790225A (zh) * 2021-01-26 2022-07-26 清华大学 一种新型内体逃逸肽及其应用
WO2023279118A2 (en) * 2021-07-02 2023-01-05 University Of Maryland, College Park Cytidine deaminases and methods of genome editing using the same
WO2023102393A1 (en) 2021-11-30 2023-06-08 Pioneer Hi-Bred International, Inc. High efficiency large scale chromosomal genome manipulation
WO2023215837A2 (en) * 2022-05-04 2023-11-09 Ohio State Innovation Foundation Compositions for delivery of agents into plant cells
EP4303305A1 (en) * 2022-07-07 2024-01-10 Kutzner, Christoph Fusion polypeptides for cell penetration and cell targeting
WO2024023578A1 (en) 2022-07-28 2024-02-01 Institut Pasteur Hsc70-4 in host-induced and spray-induced gene silencing
WO2024036190A2 (en) 2022-08-09 2024-02-15 Pioneer Hi-Bred International, Inc. Guide polynucleotide multiplexing

Family Cites Families (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4880741A (en) 1983-10-06 1989-11-14 Pfizer Inc. Process for transformation of Yarrowia lipolytica
US5071764A (en) 1983-10-06 1991-12-10 Pfizer Inc. Process for integrative transformation of yarrowia lipolytica
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US4886741A (en) 1987-12-09 1989-12-12 Microprobe Corporation Use of volume exclusion agents for the enhancement of in situ hybridization
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
EP0452269B1 (en) 1990-04-12 2002-10-09 Syngenta Participations AG Tissue-preferential promoters
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
AU651380B2 (en) 1990-07-16 1994-07-21 University Of Medicine And Dentistry Of New Jersey Selection of ribozymes that efficiently cleave target RNA
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
GB9114259D0 (en) 1991-07-02 1991-08-21 Ici Plc Plant derived enzyme and dna sequences
EP0600993B1 (en) 1991-08-27 1999-11-10 Novartis AG Proteins with insecticidal properties against homopteran insects and their use in plant protection
JPH06511152A (ja) 1991-10-04 1994-12-15 ノースカロライナ　ステイト　ユニバーシティー 病原体耐性トランスジェニック植物
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
WO1994012520A1 (en) 1992-11-20 1994-06-09 Enzon, Inc. Linker for linked fusion polypeptides
AU679525B2 (en) 1992-12-04 1997-07-03 Innovir Laboratories, Inc. Regulatable nucleic acid therapeutic and methods of use thereof
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US6063566A (en) 1994-05-13 2000-05-16 The Scripps Research Institute Catalytic RNA molecules
US5580967A (en) 1994-05-13 1996-12-03 The Scripps Research Institute Optimized catalytic DNA-cleaving ribozymes
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5688670A (en) 1994-09-01 1997-11-18 The General Hospital Corporation Self-modifying RNA molecules and methods of making
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
ATE229977T1 (de) 1996-03-26 2003-01-15 Razvan T Radulescu Peptide mit antiproliferativen eigenschaften
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
US6828099B2 (en) 1998-02-02 2004-12-07 Odyssey Thera Inc. Protein fragment complementation assay (PCA) for the detection of protein-protein, protein-small molecule and protein nucleic acid interactions based on the E. coli TEM-1 β-Lactamase
ATE336580T1 (de) 1998-02-26 2006-09-15 Pioneer Hi Bred Int Mais met-1 promoter
CA2332628C (en) 1998-08-20 2005-04-12 Pioneer Hi-Bred International, Inc. Seed-preferred promoters
AU5788299A (en) 1998-08-28 2000-03-21 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from (end) genes
EP1135531B1 (en) 1998-12-01 2007-05-02 Phylonix Pharmaceuticals Inc. Methods for introducing heterologous cells into fish
GB0113798D0 (en) 2001-06-06 2001-07-25 Chiron Spa Antigens and vectors for vaccination
WO2003052067A2 (en) 2001-12-13 2003-06-26 University Of Virginia Patent Foundation Vector mediated organelle transfection
ES2566487T3 (es) 2002-04-12 2016-04-13 Omeros Corporation Método de identificación de compuestos que interaccionan con proteínas transmembrana
US7132230B2 (en) 2002-06-28 2006-11-07 Fujikin Incorporated Cytotoxic assay and new established cell line of sturgeon origin
US20110059496A1 (en) 2003-06-25 2011-03-10 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
US7459546B2 (en) 2003-06-25 2008-12-02 E.I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase regulatory sequences for gene expression in oleaginous yeast
US7202356B2 (en) 2003-11-14 2007-04-10 E. I. Du Pont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
US7264949B2 (en) 2004-09-15 2007-09-04 E.I. Du Pont De Nemours And Company Glycerol-3-phosphate o-acyltransferase promoter for gene expression in oleaginous yeast
US20060094102A1 (en) 2004-11-04 2006-05-04 Zhixiong Xue Ammonium transporter promoter for gene expression in oleaginous yeast
ES2398918T3 (es) 2005-08-26 2013-03-22 Dupont Nutrition Biosciences Aps Un método y una ordenación para soportar verticalmente elementos de resistencia eléctrica pendientes
EP1996713B1 (en) 2006-03-20 2012-08-29 Max-Delbrück-Centrum für Molekulare Medizin (MDC) Use of pol iii promoters for controlled expression of therapeutic proteins
HUE028692T2 (en) 2007-04-03 2016-12-28 Du Pont Multi-uses and their use in the production of polyunsaturated fatty acids
CA2700008A1 (en) 2007-08-01 2009-02-05 Bioglow Inc. Bioluminescent plants comprising bacterial lux operon and methods of making same
US9216235B2 (en) 2007-08-14 2015-12-22 Cook Medical Technologies Llc Photoactivated crosslinking of a protein or peptide
JP5552053B2 (ja) * 2007-09-25 2014-07-16 パストラル グリーンハウス ガス リサーチ リミテッド ファージφＭＲＵポリヌクレオチドおよびポリペプチドならびにその使用
US8889394B2 (en) 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
US9187762B2 (en) 2010-08-13 2015-11-17 Pioneer Hi-Bred International, Inc. Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (HPPD) activity
US8642744B2 (en) 2010-10-28 2014-02-04 Agilent Technologies, Inc. Crosslinking reagent for analysis of protein-protein interaction
US8580922B2 (en) 2011-03-04 2013-11-12 Shire Human Genetic Therapies, Inc. Peptide linkers for polypeptide compositions and methods for using same
EP2691518A1 (en) 2011-03-31 2014-02-05 E.I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
US8906650B2 (en) 2011-04-01 2014-12-09 E I Du Pont De Nemours And Company Yarrowia esterase/lipase promoter regions for gene expression in yeast
WO2012138613A1 (en) 2011-04-05 2012-10-11 E. I. Du Pont De Nemours And Company Yarrowia n-alkane-hydroxylating cytochrome p450 promoter regions for gene expression in yeast
EP2702148A1 (en) 2011-04-07 2014-03-05 E.I. Du Pont De Nemours And Company Yarrowia peroxisomal 2,4-dienoyl-coa reductase promoter regions for gene expression in yeast
EP2750686B1 (en) 2011-06-17 2017-06-14 Shire Human Genetic Therapies, Inc. Polypeptide comprising frataxin and a c-terminal mitochondria penetrating peptide for use in the treatment of friedreich's ataxia
DE202013012242U1 (de) 2012-05-25 2016-02-02 Emmanuelle Charpentier Zusammensetzungen für die durch RNA gesteuerte Modifikation einer Ziel-DNA und für die durch RNA gesteuerte Modulation der Transkription
DE202013012597U1 (de) * 2012-10-23 2017-11-21 Toolgen, Inc. Zusammensetzung zum Spalten einer Ziel-DNA, umfassend eine für die Ziel-DNA spezifische guide-RNA und eine Cas-Protein-codierende Nukleinsäure oder ein Cas-Protein, sowie deren Verwendung
PL3138910T3 (pl) * 2012-12-06 2018-01-31 Sigma Aldrich Co Llc Oparta na CRISPR modyfikacja i regulacja genomu
US20140189896A1 (en) 2012-12-12 2014-07-03 Feng Zhang Crispr-cas component systems, methods and compositions for sequence manipulation
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
DK2935601T3 (da) 2012-12-21 2018-06-18 Du Pont Rekombinante mikrobeceller, der fremstiller mindst 28 % eicosapentaensyre som tørvægt
WO2014165825A2 (en) * 2013-04-04 2014-10-09 President And Fellows Of Harvard College Therapeutic uses of genome editing with crispr/cas systems
EP3611268A1 (en) 2013-08-22 2020-02-19 E. I. du Pont de Nemours and Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
US9526784B2 (en) * 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US20160304893A1 (en) * 2013-12-13 2016-10-20 Cellectis Cas9 nuclease platform for microalgae genome engineering
US10513711B2 (en) 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
CN104328138A (zh) * 2014-09-30 2015-02-04 上海缔达生物科技有限公司 基因组靶标目的基因的定向敲除的方法及试剂盒
US11371050B2 (en) 2015-05-15 2022-06-28 Pioneer Hi-Bred International, Inc. Rapid characterization of Cas endonuclease systems, PAM sequences and guide RNA elements

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