DK2078071T3 - Rationelt udformede cellekulturmedier - Google Patents
Rationelt udformede cellekulturmedier Download PDFInfo
- Publication number
- DK2078071T3 DK2078071T3 DK07868688.8T DK07868688T DK2078071T3 DK 2078071 T3 DK2078071 T3 DK 2078071T3 DK 07868688 T DK07868688 T DK 07868688T DK 2078071 T3 DK2078071 T3 DK 2078071T3
- Authority
- DK
- Denmark
- Prior art keywords
- cell culture
- cell
- medium
- polypeptide
- cells
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Claims (14)
1. Cellekulturmedium omfattende mellem 7 mM og 30 mM leucin, mellem 7 mM og 30 mM lysin, mellem 7 mM og 30 mM threonin, mellem 7 mM og 30 mM prolin og mellem 7 mM og 30 mM valin.
2. Cellekulturmedium ifølge krav 1, som endvidere omfatter mere end eller lig med 3 mM tyrosin.
3. Cellekulturmedium ifølge krav 1 eller 2, hvori den kombinerede koncentration af leucin, lysin, threonin, prolin og valin ligger mellem 60% og 80% af koncentrationen af de samlede essentielle aminosyrer i cellekulturmediet.
4. Cellekulturmedium ifølge krav 1 eller 2, hvori den kombinerede koncentration af de essentielle aminosyrer ligger mellem 30% og 50% af koncentrationen af de samlede aminosyrer.
5. Cellekulturmedium ifølge et hvilket som helst af kravene 1 til 4 omfattende en totalkoncentration af aminosyrer mellem 120 mM og 350 mM.
6. Cellekulturmedium ifølge et hvilket som helst af kravene 1 til 6 omfattende en totalkoncentration af aminosyrer på mere end 140 mM.
7. Fremgangsmåde til fremstilling af et polypeptid i en cellekultur omfattende: (1) tilvejebringelse af en cellekultur, der omfatter: a. celler, der omfatter en nukleinsyre, som koder for et polypeptid af interesse; og b. et cellekulturmedium ifølge et hvilket som helst af kravene 1 til 6; og (2) opretholdelse af cellekulturen under betingelser, der tillader ekspression af polypeptidet af interesse.
8. Fremgangsmåde ifølge krav 7, som endvidere omfatter en fremgangsmåde til udvinding af et renset polypeptid fra en indføringsvæske omfattende følgende trin: at lade indføringsvæsken passere gennem et medium i en søjle under driftsbetingelser, der bringer mediet til at binde mindst 2,8 mg polypeptid pr. ml medium, idet mediet er valgt fra den gruppe, der består af et ladet ionbytter- medium, en hydrofob vekselvirkningschromatografiharpiks og en immobiliseret metalaffinitetschromatografiharpiks; og at udvinde det rensede polypeptid fra det fra søjlen udstrømmende materiale.
9. Fremgangsmåde ifølge krav 7, som endvidere omfatter en fremgangsmåde til udvinding af et renset polypeptid fra en indføringsvæske omfattende følgende trin: at lade indføringsvæsken passere gennem et medium i en søjle under driftsbetingelser, der er defineret ved en fordelingskoefficient på mindst 0,1; og at udvinde den rensede polypeptid fra det fra søjlen udstrømmende materiale.
10. Fremgangsmåde til fremstilling af et polypeptid i en cellekultur omfattende: (1) tilvejebringelse af en cellekultur, der omfatter: a. celler, der omfatter en nukleinsyre, som koder for et polypeptid af interesse; og b. et start-cellekulturmedium, idet start-cellekulturmediets rumfang er 60-99% af et ønsket cellekulturmedierumfang; (2) tilvejebringelse af et cellekulturfodringsmedium til cellekulturen ifølge trin (1), idet cellekulturfodringsmediets rumfang er 1-40% af det ønskede cellekulturmedierumfang, og idet det fremkomne ønskede cellekulturmedium er et cellekulturmedium ifølge et hvilket som helst af kravene 1 til 6; og (3) opretholdelse af cellekulturen under betingelser, der tillader ekspression af polypeptidet af interesse.
11. Fremgangsmåde ifølge et hvilket som helst af kravene 7 til 10, ved hvilken cellekulturen er en cellekultur i stor målestok.
12. Fremgangsmåde ifølge et hvilket som helst af kravene 7 til 11, ved hvilken cellerne er dyreceller.
13. Fremgangsmåde ifølge et hvilket som helst af kravene 7 og 10 til 12, ved hvilken polypeptidet renses.
14. Fremgangsmåde ifølge et hvilket som helst af kravene 7 til 13, ved hvilken mediet er et defineret medium.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US85828906P | 2006-11-08 | 2006-11-08 | |
| PCT/US2007/083947 WO2008063892A2 (en) | 2006-11-08 | 2007-11-07 | Rationally designed media for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2078071T3 true DK2078071T3 (da) | 2015-04-20 |
Family
ID=39430451
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK07868688.8T DK2078071T3 (da) | 2006-11-08 | 2007-11-07 | Rationelt udformede cellekulturmedier |
Country Status (26)
| Country | Link |
|---|---|
| US (2) | US8232075B2 (da) |
| EP (2) | EP2921552A1 (da) |
| JP (2) | JP5564259B2 (da) |
| KR (1) | KR101523782B1 (da) |
| CN (2) | CN101535469A (da) |
| AR (1) | AR063623A1 (da) |
| AU (1) | AU2007323978B2 (da) |
| BR (1) | BRPI0718713B1 (da) |
| CA (1) | CA2668771C (da) |
| CL (1) | CL2007003221A1 (da) |
| CR (1) | CR10781A (da) |
| DK (1) | DK2078071T3 (da) |
| EC (1) | ECSP099313A (da) |
| ES (1) | ES2538986T3 (da) |
| HK (1) | HK1132300A1 (da) |
| IL (1) | IL198486A (da) |
| MX (1) | MX2009004974A (da) |
| NO (1) | NO20091607L (da) |
| PE (1) | PE20081655A1 (da) |
| PL (1) | PL2078071T3 (da) |
| PT (1) | PT2078071E (da) |
| RU (1) | RU2520810C2 (da) |
| SI (1) | SI2078071T1 (da) |
| TW (1) | TW200827445A (da) |
| WO (1) | WO2008063892A2 (da) |
| ZA (1) | ZA200903170B (da) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2538986T3 (es) * | 2006-11-08 | 2015-06-25 | Wyeth Llc | Medios diseñados racionalmente para un cultivo celular |
| KR101574355B1 (ko) | 2007-04-26 | 2015-12-11 | 추가이 세이야쿠 가부시키가이샤 | 고농도 아미노산 함유 배지를 사용한 세포의 배양 방법 |
| US20090042253A1 (en) * | 2007-08-09 | 2009-02-12 | Wyeth | Use of perfusion to enhance production of fed-batch cell culture in bioreactors |
| US8178318B2 (en) * | 2008-08-06 | 2012-05-15 | Praxair Technology, Inc. | Method for controlling pH, osmolality and dissolved carbon dioxide levels in a mammalian cell culture process to enhance cell viability and biologic product yield |
| US20100035342A1 (en) * | 2008-08-06 | 2010-02-11 | Cheng Alan T Y | METHOD FOR CONTROLLING pH, OSMOLALITY AND DISSOLVED CARBON DIOXIDE LEVELS IN A MAMMALIAN CELL CULTURE PROCESS TO ENHANCE CELL VIABILITY AND BIOLOGIC PRODUCT YIELD |
| KR20190018041A (ko) | 2009-08-06 | 2019-02-20 | 제넨테크, 인크. | 단백질 정제 시의 바이러스 제거의 개선방법 |
| US20110262965A1 (en) * | 2010-04-23 | 2011-10-27 | Life Technologies Corporation | Cell culture medium comprising small peptides |
| EP3330370B1 (en) | 2010-04-26 | 2021-02-24 | Novartis AG | Process for cultivation of cho cells |
| US9428727B2 (en) * | 2010-04-26 | 2016-08-30 | Novartis Ag | Cell culture medium |
| EP2447717B1 (en) * | 2010-10-27 | 2013-09-18 | Lonza Biologics plc. | Rapid method for targeted cell (line) selection |
| PT105484A (pt) | 2011-01-14 | 2012-07-16 | Univ Nova De Lisboa | Um método de ambientómica funcional para engenharia de meio de cultura celular |
| WO2013006479A2 (en) | 2011-07-01 | 2013-01-10 | Amgen Inc. | Mammalian cell culture |
| US9475858B2 (en) * | 2011-07-08 | 2016-10-25 | Momenta Pharmaceuticals, Inc. | Cell culture process |
| EP4026596A1 (en) * | 2013-03-14 | 2022-07-13 | Amgen, Inc | Removal of leaked affinity purification ligand |
| AR095196A1 (es) * | 2013-03-15 | 2015-09-30 | Regeneron Pharma | Medio de cultivo celular libre de suero |
| TW202330904A (zh) | 2015-08-04 | 2023-08-01 | 美商再生元醫藥公司 | 補充牛磺酸之細胞培養基及用法 |
| CN108473945A (zh) * | 2015-11-17 | 2018-08-31 | 辉瑞公司 | 用于在细菌细胞培养物中生产多糖的培养基和发酵方法 |
| US20190112572A1 (en) * | 2016-04-05 | 2019-04-18 | Pfizer Inc. | Cell culture process |
| CN106096077B (zh) * | 2016-05-26 | 2019-05-21 | 新乡医学院 | 培养基优化方法 |
| JP6314201B1 (ja) * | 2016-11-21 | 2018-04-18 | テラファーマ株式会社 | 樹状細胞洗浄液及びこれを用いた樹状細胞の洗浄方法、並びに樹状細胞含有組成物の調製方法 |
| US20190381182A1 (en) * | 2017-02-08 | 2019-12-19 | Pfizer Inc. | Large scale production process for capped and un-capped antibody cysteines and their use in therapeutic protein conjugation |
| GB201708655D0 (en) * | 2017-05-31 | 2017-07-12 | Ucb Biopharma Sprl | Cell culture methods |
| KR20200070218A (ko) * | 2017-10-16 | 2020-06-17 | 리제너론 파마슈티칼스 인코포레이티드 | 세포 배양에서 공정 변수를 제어하기 위한 원위치 라만 분광법 시스템 및 방법 |
| US20210171901A1 (en) * | 2017-11-16 | 2021-06-10 | Life Technologies Corporation | Streamlined methods for making liquid media |
| JP6385607B2 (ja) * | 2018-02-13 | 2018-09-05 | テラファーマ株式会社 | 樹状細胞ワクチン |
| CN108823173A (zh) * | 2018-07-24 | 2018-11-16 | 郑州伊美诺生物技术有限公司 | 用于杂交瘤细胞培养的补料培养基及其制备方法 |
| CN109576203B (zh) * | 2018-12-20 | 2021-03-23 | 吉林省浦生泰生物技术有限责任公司 | 一种基于中药添加剂的复合益生菌培养基及其制备方法 |
| CN111748527B (zh) * | 2020-05-15 | 2021-01-29 | 上海多宁生物科技有限公司 | 化学成分限定高效补料培养基及其制备方法和应用 |
| CN113450882B (zh) * | 2020-09-27 | 2022-03-01 | 深圳太力生物技术有限责任公司 | 一种基于人工智能的基础培养基配方开发方法及系统 |
| JP2022060169A (ja) | 2020-10-02 | 2022-04-14 | ファイザー・インク | Ｒｓｖ ｆタンパク質生産のための細胞培養工程 |
| WO2022254319A1 (en) | 2021-06-01 | 2022-12-08 | Pfizer Inc. | Cell culture method for producing sfgfr3 polypeptide |
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| US4282326A (en) | 1978-10-12 | 1981-08-04 | Jeanne Moldenhauer | Cell culture medium supplement |
| US4510245A (en) * | 1982-11-18 | 1985-04-09 | Chiron Corporation | Adenovirus promoter system |
| US5168062A (en) * | 1985-01-30 | 1992-12-01 | University Of Iowa Research Foundation | Transfer vectors and microorganisms containing human cytomegalovirus immediate-early promoter-regulatory DNA sequence |
| US4968615A (en) * | 1985-12-18 | 1990-11-06 | Ciba-Geigy Corporation | Deoxyribonucleic acid segment from a virus |
| GB8927546D0 (en) | 1989-12-06 | 1990-02-07 | Ciba Geigy | Process for the production of biologically active tgf-beta |
| US5122469A (en) * | 1990-10-03 | 1992-06-16 | Genentech, Inc. | Method for culturing Chinese hamster ovary cells to improve production of recombinant proteins |
| GB2251249B (en) | 1990-12-28 | 1995-06-21 | Mogam Biotech Res Inst | High-density medium for animal cell culture |
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| US5856179A (en) | 1994-03-10 | 1999-01-05 | Genentech, Inc. | Polypeptide production in animal cell culture |
| RU2141531C1 (ru) * | 1999-05-26 | 1999-11-20 | Российское акционерное общество открытого типа "Биопрепарат" | Способ получения рекомбинантного инсулина человека |
| AU7950400A (en) | 1999-10-19 | 2001-04-30 | Kyowa Hakko Kogyo Co. Ltd. | Process for producing polypeptide |
| DK1623019T4 (da) * | 2003-05-15 | 2017-03-27 | Wyeth Llc | Begrænset glucosetilførsel til dyrecellekultur |
| US20070128691A1 (en) | 2003-10-09 | 2007-06-07 | Ryosuke Nakano | Genomically modified cell neutralized to serum-free system |
| TWI384069B (zh) | 2004-08-27 | 2013-02-01 | Pfizer Ireland Pharmaceuticals | 多胜肽之製法 |
| TWI374935B (en) | 2004-08-27 | 2012-10-21 | Pfizer Ireland Pharmaceuticals | Production of α-abeta |
| US7300773B2 (en) * | 2004-08-27 | 2007-11-27 | Wyeth Research Ireland Limited | Production of TNFR-Ig |
| ES2380485T3 (es) | 2005-02-11 | 2012-05-14 | Novo Nordisk Health Care Ag | Producción de un polipéptido en un lÃquido de cultivo sin suero con hidrolizado de proteÃnas vegetales |
| HUE049797T2 (hu) * | 2005-03-11 | 2020-10-28 | Wyeth Llc | Gyenge megoszlási kromatográfiás eljárás |
| ES2538986T3 (es) | 2006-11-08 | 2015-06-25 | Wyeth Llc | Medios diseñados racionalmente para un cultivo celular |
-
2007
- 2007-11-07 ES ES07868688.8T patent/ES2538986T3/es active Active
- 2007-11-07 SI SI200731638T patent/SI2078071T1/sl unknown
- 2007-11-07 CN CNA2007800411581A patent/CN101535469A/zh active Pending
- 2007-11-07 BR BRPI0718713A patent/BRPI0718713B1/pt active IP Right Grant
- 2007-11-07 CN CN201410370624.2A patent/CN104152395B/zh active Active
- 2007-11-07 CA CA2668771A patent/CA2668771C/en active Active
- 2007-11-07 DK DK07868688.8T patent/DK2078071T3/da active
- 2007-11-07 RU RU2009114855/10A patent/RU2520810C2/ru active
- 2007-11-07 WO PCT/US2007/083947 patent/WO2008063892A2/en active Application Filing
- 2007-11-07 EP EP15159383.7A patent/EP2921552A1/en active Pending
- 2007-11-07 KR KR1020097011505A patent/KR101523782B1/ko active IP Right Grant
- 2007-11-07 TW TW096142017A patent/TW200827445A/zh unknown
- 2007-11-07 PT PT78686888T patent/PT2078071E/pt unknown
- 2007-11-07 AU AU2007323978A patent/AU2007323978B2/en active Active
- 2007-11-07 EP EP07868688.8A patent/EP2078071B1/en not_active Revoked
- 2007-11-07 MX MX2009004974A patent/MX2009004974A/es active IP Right Grant
- 2007-11-07 JP JP2009536462A patent/JP5564259B2/ja active Active
- 2007-11-07 PL PL07868688T patent/PL2078071T3/pl unknown
- 2007-11-08 US US11/936,866 patent/US8232075B2/en active Active
- 2007-11-08 AR ARP070104982A patent/AR063623A1/es unknown
- 2007-11-08 CL CL200703221A patent/CL2007003221A1/es unknown
- 2007-11-08 PE PE2007001542A patent/PE20081655A1/es not_active Application Discontinuation
-
2009
- 2009-04-22 NO NO20091607A patent/NO20091607L/no not_active Application Discontinuation
- 2009-04-30 IL IL198486A patent/IL198486A/en active IP Right Grant
- 2009-05-07 EC EC2009009313A patent/ECSP099313A/es unknown
- 2009-05-07 ZA ZA200903170A patent/ZA200903170B/xx unknown
- 2009-05-08 CR CR10781A patent/CR10781A/es unknown
- 2009-12-28 HK HK09112222.6A patent/HK1132300A1/xx unknown
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2012
- 2012-07-30 US US13/561,395 patent/US20130084593A1/en not_active Abandoned
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2014
- 2014-02-19 JP JP2014029923A patent/JP2014128276A/ja not_active Withdrawn
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