DK180609B1 - Method and composition having a transporter molecule conjugated to an anti- sense morpholino oligomer for suppressing expression of fish dead end gene - Google Patents

Method and composition having a transporter molecule conjugated to an anti- sense morpholino oligomer for suppressing expression of fish dead end gene Download PDF

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Publication number
DK180609B1
DK180609B1 DKPA201670401A DKPA201670401A DK180609B1 DK 180609 B1 DK180609 B1 DK 180609B1 DK PA201670401 A DKPA201670401 A DK PA201670401A DK PA201670401 A DKPA201670401 A DK PA201670401A DK 180609 B1 DK180609 B1 DK 180609B1
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Denmark
Prior art keywords
fish
eggs
egg
dead end
fertilized
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DKPA201670401A
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Danish (da)
English (en)
Inventor
Zohar Yonathan
Wong Ten-Tsao
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Univ Of Maryland At Baltimore County
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Publication of DK201670401A1 publication Critical patent/DK201670401A1/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/05—Animals modified by non-integrating nucleic acids, e.g. antisense, RNAi, morpholino, episomal vector, for non-therapeutic purpose
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/40—Fish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3233—Morpholino-type ring
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
    • Y02A90/40—Monitoring or fighting invasive species

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Feed For Specific Animals (AREA)
  • Fodder In General (AREA)
DKPA201670401A 2013-11-15 2016-06-06 Method and composition having a transporter molecule conjugated to an anti- sense morpholino oligomer for suppressing expression of fish dead end gene DK180609B1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361904652P 2013-11-15 2013-11-15
US201461968458P 2014-03-21 2014-03-21
US201462050815P 2014-09-16 2014-09-16
PCT/US2014/065698 WO2015073819A1 (en) 2013-11-15 2014-11-14 Method of producing infertile fish and egg-producing aquatic animals and of delivering compounds into eggs and embryos

Publications (2)

Publication Number Publication Date
DK201670401A1 DK201670401A1 (en) 2016-07-04
DK180609B1 true DK180609B1 (en) 2021-10-14

Family

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Application Number Title Priority Date Filing Date
DKPA201670401A DK180609B1 (en) 2013-11-15 2016-06-06 Method and composition having a transporter molecule conjugated to an anti- sense morpholino oligomer for suppressing expression of fish dead end gene

Country Status (18)

Country Link
US (3) US9999208B2 (enExample)
EP (1) EP3068216B1 (enExample)
JP (1) JP6669650B2 (enExample)
CN (1) CN105764333B (enExample)
AU (1) AU2014348473B2 (enExample)
CA (1) CA2929496C (enExample)
CL (1) CL2016001113A1 (enExample)
DK (1) DK180609B1 (enExample)
ES (1) ES2981867T3 (enExample)
FI (1) FI3068216T3 (enExample)
HR (1) HRP20240918T1 (enExample)
IL (1) IL245293B (enExample)
MX (1) MX369329B (enExample)
NZ (1) NZ719421A (enExample)
PL (1) PL3068216T3 (enExample)
PT (1) PT3068216T (enExample)
RU (1) RU2723037C2 (enExample)
WO (1) WO2015073819A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
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WO2015073819A1 (en) 2013-11-15 2015-05-21 University Of Maryland Baltimore County Method of producing infertile fish and egg-producing aquatic animals and of delivering compounds into eggs and embryos
BR112017000925B1 (pt) 2014-07-14 2023-10-10 Washington State University Método de reprodução de porcos, método para gerar um porco sem nenhuma célula espermatogônica funcional, método de produção de um macho receptor e método de produção de porcos
RU2741645C2 (ru) * 2015-05-19 2021-01-28 Юниверсити Оф Мэрилэнд Балтимор Каунти Способы доставки агента в икринки рыб
CN106614142B (zh) * 2016-12-28 2019-12-03 浙江省海洋水产研究所 一种杂交不育黄姑鱼的生产方法
CN109864017A (zh) * 2017-12-05 2019-06-11 柳州市渔业技术推广站 山塘水库高产高效渔业养殖方法
PL3723479T3 (pl) * 2017-12-15 2024-10-07 Acd Pharmaceuticals As Sposoby produkcji sterylnych ryb
CN108668984B (zh) * 2018-04-15 2021-02-12 广西壮族自治区水产科学研究院 一种罗非鱼苗种高密度运输方法
CN108739537B (zh) * 2018-06-06 2021-02-09 江苏省淡水水产研究所 一种粉白体色斑点叉尾鮰家系制种方法
BR112021020349A2 (pt) * 2019-04-09 2021-12-14 Mayo Found Medical Education & Res Peixe estéril
CN112553205B (zh) * 2020-12-29 2022-09-13 中国水产科学研究院淡水渔业研究中心 一种抑制黄颡鱼性腺发育的反义rna序列组合和方法及应用
CN114982678B (zh) * 2022-07-21 2024-02-23 新疆额河生态养殖科技有限公司 一种北极茴鱼人工繁殖操作方法

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US5780448A (en) * 1995-11-07 1998-07-14 Ottawa Civic Hospital Loeb Research DNA-based vaccination of fish
US7115794B2 (en) * 2003-05-19 2006-10-03 University Of Hawaii Method for direct nucleic acid uptake
US7194978B2 (en) * 2003-08-22 2007-03-27 University Of Maryland Biotechnology Institute Inducing sterility in fish by disrupting the development of the GnRH system
CN1294808C (zh) * 2003-09-26 2007-01-17 中国科学院水生生物研究所 不育转基因鱼的制备方法
CN1274822C (zh) * 2004-02-19 2006-09-13 武汉康祥科技发展有限公司 活体化学诱导鱼类雌核发育方法
CN103898161A (zh) * 2007-01-26 2014-07-02 辛那杰瓦生物制药股份有限公司 在禽类中进行转基因表达
US7935816B2 (en) 2007-10-25 2011-05-03 Gene Tools, Llc Molecular transporter compositions comprising dendrimeric oligoguanidine with a triazine core that facilitate delivery into cells in vivo
US8383880B2 (en) * 2009-02-18 2013-02-26 Academia Sinica Infertility control of genetically modified fish
CA2789454A1 (en) * 2010-02-09 2011-08-18 National University Corporation Hokkaido University Method for acquiring genetically identical gamete from lethal fish haploid-derived germ cell via germ line chimera
RU2422529C1 (ru) * 2010-02-12 2011-06-27 Учреждение Российской академии наук Институт цитологии и генетики Сибирского отделения РАН (ИЦиГ СО РАН) ГЕННО-ИНЖЕНЕРНАЯ КОНСТРУКЦИЯ pGoatcasGCSF, ОБЕСПЕЧИВАЮЩАЯ ПРОДУКЦИЮ ГРАНУЛОЦИТ-КОЛОНИЕСТИМУЛИРУЮЩЕГО ФАКТОРА ЧЕЛОВЕКА В МОЛОКО ТРАНСГЕННЫХ ЖИВОТНЫХ
CA2825994A1 (en) * 2011-02-01 2012-08-09 Ten-Tsao WONG Efficient sterilization of fish by disruption of germ cell development
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WO2015073819A1 (en) 2013-11-15 2015-05-21 University Of Maryland Baltimore County Method of producing infertile fish and egg-producing aquatic animals and of delivering compounds into eggs and embryos
RU2741645C2 (ru) * 2015-05-19 2021-01-28 Юниверсити Оф Мэрилэнд Балтимор Каунти Способы доставки агента в икринки рыб

Also Published As

Publication number Publication date
EP3068216A1 (en) 2016-09-21
MX369329B (es) 2019-11-05
AU2014348473B2 (en) 2018-06-14
PL3068216T3 (pl) 2024-10-14
BR112016011080A2 (pt) 2017-12-05
CA2929496C (en) 2022-08-23
EP3068216B1 (en) 2024-04-10
ES2981867T3 (es) 2024-10-10
WO2015073819A1 (en) 2015-05-21
CN105764333A (zh) 2016-07-13
DK201670401A1 (en) 2016-07-04
FI3068216T3 (fi) 2024-07-10
RU2016123427A (ru) 2017-12-20
CA2929496A1 (en) 2015-05-21
US9999208B2 (en) 2018-06-19
PT3068216T (pt) 2024-07-12
US20160286768A1 (en) 2016-10-06
US20190104710A1 (en) 2019-04-11
HRP20240918T1 (hr) 2024-10-11
EP3068216A4 (en) 2017-10-11
IL245293A0 (en) 2016-06-30
JP2017502651A (ja) 2017-01-26
MX2016006315A (es) 2017-01-19
NZ719421A (en) 2022-09-30
US20180288984A1 (en) 2018-10-11
CL2016001113A1 (es) 2017-01-06
JP6669650B2 (ja) 2020-03-18
CN105764333B (zh) 2021-09-03
US10136621B2 (en) 2018-11-27
AU2014348473A1 (en) 2016-05-19
RU2723037C2 (ru) 2020-06-08
IL245293B (en) 2020-04-30

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Effective date: 20211014