CN105764333B - 生产不育的鱼和产卵水生动物及递送化合物至卵和胚胎中的方法 - Google Patents

生产不育的鱼和产卵水生动物及递送化合物至卵和胚胎中的方法 Download PDF

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CN105764333B
CN105764333B CN201480062691.6A CN201480062691A CN105764333B CN 105764333 B CN105764333 B CN 105764333B CN 201480062691 A CN201480062691 A CN 201480062691A CN 105764333 B CN105764333 B CN 105764333B
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fish
eggs
oligomer
antisense morpholino
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CN105764333A (zh
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约纳坦·祖海尔
翁天佐
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University of Maryland Baltimore County UMBC
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/05—Animals modified by non-integrating nucleic acids, e.g. antisense, RNAi, morpholino, episomal vector, for non-therapeutic purpose
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/40—Fish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/11—Antisense
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/323—Chemical structure of the sugar modified ring structure
    • C12N2310/3233—Morpholino-type ring
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
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    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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    • Y02A90/00—Technologies having an indirect contribution to adaptation to climate change
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  • Developmental Biology & Embryology (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Marine Sciences & Fisheries (AREA)
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CN201480062691.6A 2013-11-15 2014-11-14 生产不育的鱼和产卵水生动物及递送化合物至卵和胚胎中的方法 Active CN105764333B (zh)

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Application Number Priority Date Filing Date Title
US201361904652P 2013-11-15 2013-11-15
US61/904,652 2013-11-15
US201461968458P 2014-03-21 2014-03-21
US61/968,458 2014-03-21
US201462050815P 2014-09-16 2014-09-16
US62/050,815 2014-09-16
PCT/US2014/065698 WO2015073819A1 (en) 2013-11-15 2014-11-14 Method of producing infertile fish and egg-producing aquatic animals and of delivering compounds into eggs and embryos

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CN105764333B true CN105764333B (zh) 2021-09-03

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AU (1) AU2014348473B2 (enExample)
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CL (1) CL2016001113A1 (enExample)
DK (1) DK180609B1 (enExample)
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Publication number Priority date Publication date Assignee Title
EP3068216B1 (en) 2013-11-15 2024-04-10 University of Maryland at Baltimore County Method of producing infertile fish and egg-producing aquatic animals and of delivering compounds into eggs and embryos
AU2015289799B2 (en) 2014-07-14 2018-07-26 The University Court Of The University Of Edinburgh NANOS knock-out that ablates germline cells
RU2741645C2 (ru) * 2015-05-19 2021-01-28 Юниверсити Оф Мэрилэнд Балтимор Каунти Способы доставки агента в икринки рыб
CN106614142B (zh) * 2016-12-28 2019-12-03 浙江省海洋水产研究所 一种杂交不育黄姑鱼的生产方法
CN109864017A (zh) * 2017-12-05 2019-06-11 柳州市渔业技术推广站 山塘水库高产高效渔业养殖方法
ES2987265T3 (es) 2017-12-15 2024-11-14 Acd Pharmaceuticals As Métodos para la producción de peces estériles
CN108668984B (zh) * 2018-04-15 2021-02-12 广西壮族自治区水产科学研究院 一种罗非鱼苗种高密度运输方法
CN108739537B (zh) * 2018-06-06 2021-02-09 江苏省淡水水产研究所 一种粉白体色斑点叉尾鮰家系制种方法
JP2022526836A (ja) * 2019-04-09 2022-05-26 リコンビネティクス・インコーポレイテッド 不稔魚
CN112553205B (zh) * 2020-12-29 2022-09-13 中国水产科学研究院淡水渔业研究中心 一种抑制黄颡鱼性腺发育的反义rna序列组合和方法及应用
CN114982678B (zh) * 2022-07-21 2024-02-23 新疆额河生态养殖科技有限公司 一种北极茴鱼人工繁殖操作方法

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CN1528131A (zh) * 2003-09-26 2004-09-15 �й���ѧԺ�����о��� 不育转基因鱼的制备方法
CN1559201A (zh) * 2004-02-19 2005-01-05 武汉康祥科技发展有限公司 活体化学诱导鱼类雌核发育方法
WO2005001028A2 (en) * 2003-05-19 2005-01-06 University Of Hawaii Method for direct nucleic acid uptake
CN101652475A (zh) * 2007-01-26 2010-02-17 辛那杰瓦生物制药股份有限公司 在禽类中进行转基因表达
US7935816B2 (en) * 2007-10-25 2011-05-03 Gene Tools, Llc Molecular transporter compositions comprising dendrimeric oligoguanidine with a triazine core that facilitate delivery into cells in vivo
EP2535404A1 (en) * 2010-02-09 2012-12-19 National University Corporation Hokkaido University Method for acquiring genetically identical gamete from lethal fish haploid-derived germ cell via germ line chimera

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RU2422529C1 (ru) * 2010-02-12 2011-06-27 Учреждение Российской академии наук Институт цитологии и генетики Сибирского отделения РАН (ИЦиГ СО РАН) ГЕННО-ИНЖЕНЕРНАЯ КОНСТРУКЦИЯ pGoatcasGCSF, ОБЕСПЕЧИВАЮЩАЯ ПРОДУКЦИЮ ГРАНУЛОЦИТ-КОЛОНИЕСТИМУЛИРУЮЩЕГО ФАКТОРА ЧЕЛОВЕКА В МОЛОКО ТРАНСГЕННЫХ ЖИВОТНЫХ
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US9538734B2 (en) 2013-03-12 2017-01-10 The General Hospital Corporation Germ cell ablation compounds and uses thereof
EP3068216B1 (en) 2013-11-15 2024-04-10 University of Maryland at Baltimore County Method of producing infertile fish and egg-producing aquatic animals and of delivering compounds into eggs and embryos
RU2741645C2 (ru) 2015-05-19 2021-01-28 Юниверсити Оф Мэрилэнд Балтимор Каунти Способы доставки агента в икринки рыб

Patent Citations (6)

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Publication number Priority date Publication date Assignee Title
WO2005001028A2 (en) * 2003-05-19 2005-01-06 University Of Hawaii Method for direct nucleic acid uptake
CN1528131A (zh) * 2003-09-26 2004-09-15 �й���ѧԺ�����о��� 不育转基因鱼的制备方法
CN1559201A (zh) * 2004-02-19 2005-01-05 武汉康祥科技发展有限公司 活体化学诱导鱼类雌核发育方法
CN101652475A (zh) * 2007-01-26 2010-02-17 辛那杰瓦生物制药股份有限公司 在禽类中进行转基因表达
US7935816B2 (en) * 2007-10-25 2011-05-03 Gene Tools, Llc Molecular transporter compositions comprising dendrimeric oligoguanidine with a triazine core that facilitate delivery into cells in vivo
EP2535404A1 (en) * 2010-02-09 2012-12-19 National University Corporation Hokkaido University Method for acquiring genetically identical gamete from lethal fish haploid-derived germ cell via germ line chimera

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US20190104710A1 (en) 2019-04-11
JP6669650B2 (ja) 2020-03-18
IL245293B (en) 2020-04-30
FI3068216T3 (fi) 2024-07-10
AU2014348473B2 (en) 2018-06-14
US20180288984A1 (en) 2018-10-11
US9999208B2 (en) 2018-06-19
NZ719421A (en) 2022-09-30
JP2017502651A (ja) 2017-01-26
EP3068216A1 (en) 2016-09-21
DK201670401A1 (en) 2016-07-04
MX2016006315A (es) 2017-01-19
IL245293A0 (en) 2016-06-30
MX369329B (es) 2019-11-05
EP3068216B1 (en) 2024-04-10
EP3068216A4 (en) 2017-10-11
WO2015073819A1 (en) 2015-05-21
US10136621B2 (en) 2018-11-27
CA2929496C (en) 2022-08-23
ES2981867T3 (es) 2024-10-10
AU2014348473A1 (en) 2016-05-19
DK180609B1 (en) 2021-10-14
PL3068216T3 (pl) 2024-10-14
US20160286768A1 (en) 2016-10-06
CL2016001113A1 (es) 2017-01-06
RU2016123427A (ru) 2017-12-20
HRP20240918T1 (hr) 2024-10-11
CN105764333A (zh) 2016-07-13
CA2929496A1 (en) 2015-05-21
BR112016011080A2 (pt) 2017-12-05
RU2723037C2 (ru) 2020-06-08
PT3068216T (pt) 2024-07-12

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