DK1758986T3 - Neurale stamceller - Google Patents
Neurale stamceller Download PDFInfo
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- DK1758986T3 DK1758986T3 DK05752405.0T DK05752405T DK1758986T3 DK 1758986 T3 DK1758986 T3 DK 1758986T3 DK 05752405 T DK05752405 T DK 05752405T DK 1758986 T3 DK1758986 T3 DK 1758986T3
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Claims (11)
1. Fremgangsmåde til fremme af den symmetriske deling af neurale stamceller, hvilken fremgangsmåde omfatter dyrkning af de neurale stamceller bundet til et substrat i en vedhæftende enkeltlagskultur i et medium indeholdende: (a) en agonist af en EGF-receptor eller (b) en agonist af en EGF-receptor og en agonist af en FGF-receptor, hvor mindst 80 % af cellerne er symmetrisk delende neurale stamceller, og hvor ikke mere end 1 % af cellerne er positive for ekspressionen af markører for modne astrocytter, neuroner og oligodendrocytter.
2. Fremgangsmåde ifølge krav 1, hvor mindst 90 % af cellerne er symmetrisk delende neurale stamceller.
3. Fremgangsmåde ifølge krav 1 hvor mindst 95 % af cellerne er symmetrisk delende neurale stamceller.
4. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 3, hvor de neurale stamceller er humane neurale stamceller.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 3, hvor de neurale stamceller er neurale stamceller fra rotter.
6. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 3, hvor de neurale stamceller er neurale stamceller fra mus.
7. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 6, hvor de neurale stamceller i populationen er kendetegnet ved, at de positive for ekspressionen af markører RC2, 3CB2 og BLBP.
8. Fremgangsmåde ifølge krav 7, hvor de neurale stamceller i populationen endvidere er kendetegnet ved, at de er positive for ekspressionen af mindst én af: - GLAST, Pax-6, de neurale prækursormarkører nestin eller vimentin, LewisX-antigenet, Musashi-1 eller prominin.
9. Fremgangsmåde ifølge krav 7 eller 8, hvor de neurale stamceller i populationen endvidere er kendetegnet ved, at de er negative for ekspressionen af mindst én af: -Oct4 eller Nanog.
10. Fremgangsmåde ifølge et hvilket som helst af kravene 7 til 9, hvor de neurale stamceller i populationen er positive for ekspressionen af Sox-2 og negative for ekspressionen af Sox-1.
11. Fremgangsmåde ifølge et hvilket som helst af kravene 7 til 10, hvor de neurale stamceller i populationen er fri for exogen nukleinsyre, der koder for et onkogen, eller hvor de neurale stamceller ikke er immortaliserede stamceller.
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| GBGB0412887.2A GB0412887D0 (en) | 2004-06-09 | 2004-06-09 | Control of neural stem cells |
| GBGB0503044.0A GB0503044D0 (en) | 2004-06-09 | 2005-02-14 | Neural stem cells |
| GBGB0505510.8A GB0505510D0 (en) | 2004-06-09 | 2005-03-17 | Neural stem cells |
| PCT/GB2005/002289 WO2005121318A2 (en) | 2004-06-09 | 2005-06-09 | Neural stem cells |
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| ATE421575T1 (de) * | 2002-12-02 | 2009-02-15 | Anges Mg Inc | Verfahren zur kultivierung neuraler stammzellen unter verwendung des hepatozytenwachstumsfaktors |
| ES2557772T3 (es) | 2004-06-09 | 2016-01-28 | The University Court Of The University Of Edinburgh | Células madre neuronales |
| GB0505510D0 (en) * | 2004-06-09 | 2005-04-27 | Univ Edinburgh | Neural stem cells |
| WO2006044204A2 (en) | 2004-10-05 | 2006-04-27 | University Of Georgia Research Foundation, Inc. | Neuronal progenitors from feeder-free human embryonic stem cell culture |
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2005
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- 2005-06-09 WO PCT/GB2005/002289 patent/WO2005121318A2/en active Search and Examination
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- 2005-06-09 NZ NZ551832A patent/NZ551832A/en unknown
- 2005-06-09 US US11/148,431 patent/US8785187B2/en active Active
- 2005-06-09 CA CA2569978A patent/CA2569978C/en active Active
- 2005-06-09 DK DK05752405.0T patent/DK1758986T3/da active
- 2005-06-09 EP EP05752405.0A patent/EP1758986B1/en active Active
- 2005-06-09 EP EP15187477.3A patent/EP3000877B1/en active Active
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- 2005-06-09 JP JP2007526548A patent/JP2008501356A/ja active Pending
- 2005-06-09 PL PL05752405T patent/PL1758986T3/pl unknown
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Also Published As
| Publication number | Publication date |
|---|---|
| CA2569978A1 (en) | 2005-12-22 |
| JP2008501356A (ja) | 2008-01-24 |
| AU2005252434B2 (en) | 2011-11-24 |
| EP1758986A2 (en) | 2007-03-07 |
| EP3000877A1 (en) | 2016-03-30 |
| IL179919A0 (en) | 2007-05-15 |
| IL179919A (en) | 2011-09-27 |
| PL1758986T3 (pl) | 2016-03-31 |
| WO2005121318A3 (en) | 2006-03-23 |
| EP1758986B1 (en) | 2015-09-30 |
| CA2569978C (en) | 2017-06-06 |
| US9309495B2 (en) | 2016-04-12 |
| US8785187B2 (en) | 2014-07-22 |
| WO2005121318A8 (en) | 2014-08-28 |
| US20060014281A1 (en) | 2006-01-19 |
| EP3000877B1 (en) | 2019-08-14 |
| GB2414995B (en) | 2006-11-15 |
| US20140370596A1 (en) | 2014-12-18 |
| AU2005252434A1 (en) | 2005-12-22 |
| GB0511770D0 (en) | 2005-07-20 |
| ES2557772T3 (es) | 2016-01-28 |
| GB2414995A (en) | 2005-12-14 |
| CN103146649B (zh) | 2018-05-22 |
| WO2005121318A2 (en) | 2005-12-22 |
| JP2012034706A (ja) | 2012-02-23 |
| CN103146649A (zh) | 2013-06-12 |
| NZ551832A (en) | 2010-01-29 |
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