DK174962B1 - Fremgangsmåde til isoelektrisk fokusering samt apparat til udøvelse af fremgangsmåden - Google Patents

Fremgangsmåde til isoelektrisk fokusering samt apparat til udøvelse af fremgangsmåden Download PDF

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Publication number
DK174962B1
DK174962B1 DK198801913A DK191388A DK174962B1 DK 174962 B1 DK174962 B1 DK 174962B1 DK 198801913 A DK198801913 A DK 198801913A DK 191388 A DK191388 A DK 191388A DK 174962 B1 DK174962 B1 DK 174962B1
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DK
Denmark
Prior art keywords
isoelectric
amphoteric
immobilized
membranes
isoelectric point
Prior art date
Application number
DK198801913A
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Danish (da)
English (en)
Other versions
DK191388D0 (da
DK191388A (da
Inventor
Daniel Michel Faupel
Pier Giorgio Righetti
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB878708746A external-priority patent/GB8708746D0/en
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of DK191388D0 publication Critical patent/DK191388D0/da
Publication of DK191388A publication Critical patent/DK191388A/da
Application granted granted Critical
Publication of DK174962B1 publication Critical patent/DK174962B1/da

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
DK198801913A 1987-04-11 1988-04-08 Fremgangsmåde til isoelektrisk fokusering samt apparat til udøvelse af fremgangsmåden DK174962B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB878708746A GB8708746D0 (en) 1987-04-11 1987-04-11 Electrophoretic process
GB8708746 1987-04-11
GB8728289 1987-12-03
GB878728289A GB8728289D0 (en) 1987-04-11 1987-12-03 Isoelectric focusing process & means for carrying out said process

Publications (3)

Publication Number Publication Date
DK191388D0 DK191388D0 (da) 1988-04-08
DK191388A DK191388A (da) 1988-10-12
DK174962B1 true DK174962B1 (da) 2004-03-29

Family

ID=26292133

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198801913A DK174962B1 (da) 1987-04-11 1988-04-08 Fremgangsmåde til isoelektrisk fokusering samt apparat til udøvelse af fremgangsmåden

Country Status (12)

Country Link
US (2) US4971670A (cs)
EP (1) EP0287513B1 (cs)
JP (1) JPH0781987B2 (cs)
CA (1) CA1335805C (cs)
DE (1) DE3876273T2 (cs)
DK (1) DK174962B1 (cs)
ES (1) ES2037273T3 (cs)
FI (1) FI97893C (cs)
GR (1) GR3007124T3 (cs)
HK (1) HK192495A (cs)
IE (1) IE62803B1 (cs)
PT (1) PT87210B (cs)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173160A (en) * 1991-02-27 1992-12-22 Board Of Regents, The University Of Texas System Analysis of carrier ampholytes using immobilized ph gradients
US5323846A (en) * 1992-01-14 1994-06-28 Fotodyne Incorporated Fluid circulator and temperature regulator
GB2267502B (en) * 1992-05-28 1997-01-22 Aligena Ag Polymeric reaction products for use in immobilized buffered gels and membranes
US20040077074A1 (en) * 1993-11-01 2004-04-22 Nanogen, Inc. Multi-chambered analysis device
US6225059B1 (en) 1993-11-01 2001-05-01 Nanogen, Inc. Advanced active electronic devices including collection electrodes for molecular biological analysis and diagnostics
US6331274B1 (en) 1993-11-01 2001-12-18 Nanogen, Inc. Advanced active circuits and devices for molecular biological analysis and diagnostics
US6375899B1 (en) 1993-11-01 2002-04-23 Nanogen, Inc. Electrophoretic buss for transport of charged materials in a multi-chamber system
US6129828A (en) * 1996-09-06 2000-10-10 Nanogen, Inc. Apparatus and methods for active biological sample preparation
US6319472B1 (en) 1993-11-01 2001-11-20 Nanogen, Inc. System including functionally separated regions in electrophoretic system
US5437774A (en) * 1993-12-30 1995-08-01 Zymogenetics, Inc. High molecular weight electrodialysis
US5480526A (en) * 1994-06-07 1996-01-02 Bio-Rad Laboratories, Inc. Methods for the desalting of biological samples: a simple approach to eliminate disturbances in isoelectric focusing caused by the presence of salts
US6403367B1 (en) * 1994-07-07 2002-06-11 Nanogen, Inc. Integrated portable biological detection system
US7857957B2 (en) * 1994-07-07 2010-12-28 Gamida For Life B.V. Integrated portable biological detection system
US6071394A (en) 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
CN1051245C (zh) * 1995-01-20 2000-04-12 清华大学 制备型等电点电泳分离方法及设备
IT1272932B (it) * 1995-01-24 1997-07-01 Pier Giorgio Righetti Reattore ad enzima immobilizzato
US5540826A (en) * 1995-03-15 1996-07-30 Protein Technologies, Inc. Multi-channel separation device
US7824532B2 (en) 1995-04-26 2010-11-02 Life Technologies Corporation Apparatus and method for electrophoresis
US5939523A (en) * 1995-07-05 1999-08-17 Gruppo Lepetit Spa Purification of dalbaheptide antibiotics by isoelectric focusing
RU2140073C1 (ru) * 1998-01-30 1999-10-20 Стоянов Александр Владимирович Способ формирования сред с заданным профилем ph
DE19831210A1 (de) * 1998-07-03 2000-01-05 Wita Gmbh Wittmann Inst Of Tec Verfahren und Vorrichtung zur zwei-dimensionalen Trennung von Biomolekülen
AUPP521298A0 (en) * 1998-08-12 1998-09-03 Life Therapeutics Limited Purification of fibrinogen
SE9803224D0 (sv) 1998-09-23 1998-09-23 Amersham Pharm Biotech Ab Method for separation of macromolecules
AUPP790698A0 (en) * 1998-12-23 1999-01-28 Life Therapeutics Limited Separation of microorganisms
AUPP790898A0 (en) 1998-12-23 1999-01-28 Life Therapeutics Limited Renal dialysis
US20050224355A1 (en) * 1999-12-23 2005-10-13 Brendon Conlan Removal of biological contaminants
US6267579B1 (en) * 1998-12-23 2001-07-31 Clinical Laboratory Development Group, Inc. Apparatus for making a gradient gel
AUPP971399A0 (en) 1999-04-12 1999-05-06 Life Therapeutics Limited Separation of plasma components
US6284115B1 (en) 1999-09-21 2001-09-04 Agilent Technologies, Inc. In-line flow through micro-dialysis apparatus and method for high performance liquid phase separations
US6758953B2 (en) 1999-10-28 2004-07-06 Nathan A. Thomas Multistage electrophoresis apparatus and method of use for the separation and purification of cells, particles and solutes
WO2001036449A1 (en) * 1999-11-15 2001-05-25 Proteome Systems Ltd Multi-compartment electrophoresis
AU1599501A (en) * 1999-11-16 2001-05-30 James T. Champagne Solution based two-dimensional separation and detection of amphoteric substances
US7077942B1 (en) 1999-12-23 2006-07-18 Gradipore Limited Removal of biological contaminants
US20080096284A1 (en) * 2000-02-08 2008-04-24 Regents Of The University Of Michigan Protein separation and analysis
WO2001068225A1 (en) * 2000-03-15 2001-09-20 Proteosys Ag Micropreparative isoelectric focussing
US6638408B1 (en) * 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
AUPQ691400A0 (en) 2000-04-14 2000-05-11 Life Therapeutics Limited Separation of micromolecules
AUPQ697300A0 (en) 2000-04-18 2000-05-11 Life Therapeutics Limited Separation apparatus
CA2405033A1 (en) 2000-04-18 2001-10-25 Gradipore Limited Electrophoresis separation and treatment of samples
GB0010957D0 (en) 2000-05-05 2000-06-28 Novartis Ag Compound & method
US6537434B1 (en) 2000-07-21 2003-03-25 Large Scale Proteomics Corporation First dimension electrophoresis separation method and apparatus
US6562213B1 (en) * 2000-08-30 2003-05-13 Ethrog Biotechnology Ltd. Electrophoresis apparatus for simultaneous loading of multiple samples
US6923896B2 (en) * 2000-09-22 2005-08-02 The Texas A&M University System Electrophoresis apparatus and method
AUPR051500A0 (en) 2000-09-29 2000-10-26 Proteome Systems Ltd Electrophoresis system
ATE473040T1 (de) * 2000-10-06 2010-07-15 Arturus Capital Ltd Multi-port-elektrophorese-trennvorrichtung und entsprechendes verfahren
AUPR222300A0 (en) * 2000-12-21 2001-01-25 Life Therapeutics Limited Electrophoresis device and method
JP2004527739A (ja) * 2001-03-08 2004-09-09 エスログ・バイオテクノロジー・リミテッド 電気泳動のための装置及び方法
EP1410029B1 (en) * 2001-07-16 2007-09-12 Protein Forest, Inc. Arrays of buffers for analysing biomolecules by their isoelectric point
GB0121189D0 (en) * 2001-08-31 2001-10-24 Diagnoswiss Sa Apparatus and method for separating an analyte
US6887362B2 (en) * 2002-02-06 2005-05-03 Nanogen, Inc. Dielectrophoretic separation and immunoassay methods on active electronic matrix devices
WO2004024752A1 (ja) * 2002-09-11 2004-03-25 Chugai Seiyaku Kabushiki Kaisha タンパク質精製方法
AU2003302418A1 (en) * 2002-11-28 2004-06-18 Arkray Inc. Method and apparatus for concentration and purification of nucleic acid
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
WO2004104548A2 (en) * 2003-05-09 2004-12-02 Invitrogen Corporation Solution phase electrophoresis device, components, and methods
EP1664398A4 (en) * 2003-09-03 2009-08-26 Shmuel Bukshpan METHODS AND DEVICES FOR REALIZING RAPID CRYSTALLIZATION OF BIOLOGICAL MOLECULES
US20050103629A1 (en) * 2003-10-07 2005-05-19 Tom Diller Isoelectric focusing gels and methods of use thereof
CN1878863A (zh) * 2003-11-10 2006-12-13 爱科来株式会社 核酸的浓缩精制方法及装置
US20050197496A1 (en) * 2004-03-04 2005-09-08 Gtc Biotherapeutics, Inc. Methods of protein fractionation using high performance tangential flow filtration
WO2005089911A1 (en) * 2004-03-17 2005-09-29 Ciphergen Biosystems, Inc. Materials, methods, and systems for separating and identifying proteins from mixtures
US7815783B2 (en) * 2004-03-17 2010-10-19 Bio-Rad Laboratories, Inc. Multi-compartment filter and method of filtering using same
US20050249667A1 (en) * 2004-03-24 2005-11-10 Tuszynski Jack A Process for treating a biological organism
US7407816B2 (en) * 2004-05-07 2008-08-05 Gentius, Inc Isoelectric particles and uses thereof
DE212005000044U1 (de) * 2004-08-25 2007-04-05 Agilent Technologies Inc., Santa Clara Elektrophoretische Separation in einem bewegten Fluid
US20060130159A1 (en) * 2004-12-09 2006-06-15 Nick Masiello Method of purifying recombinant MSP 1-42 derived from Plasmodium falciparum
JP5123847B2 (ja) 2005-05-25 2013-01-23 バイオ−ラド ラボラトリーズ インコーポレイテッド 流体工学装置
ATE534444T1 (de) * 2005-07-28 2011-12-15 Bio Rad Laboratories Trennung von proteinen auf der basis des isoelektrischen punkts unter verwendung von festphasenpuffern
US7531632B2 (en) * 2006-02-16 2009-05-12 Gtc Biotherapeutics, Inc. Clarification of transgenic milk using depth filtration
EP2013613A4 (en) * 2006-04-27 2010-09-01 Ge Healthcare Bio Sciences Ab ELECTRODE BRIDGE
US20080220442A1 (en) * 2006-12-06 2008-09-11 Proteinics Difference detection methods using isoelectric focusing chips
US8366899B2 (en) * 2007-06-22 2013-02-05 Massachusetts Institute Of Technology Isoelectric focusing systems and methods
CA2699954A1 (en) * 2007-10-09 2009-04-16 Dalhousie University Apparatus for purifying molecules
US8282803B2 (en) 2009-03-25 2012-10-09 Bio-Rad Laboratories, Inc. Isoelectric focusing tray and electrode assembly for alternate gel strip orientations
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
US9234875B2 (en) 2011-11-04 2016-01-12 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
WO2013067477A1 (en) * 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of ph
WO2013078236A1 (en) 2011-11-22 2013-05-30 Haralampu Stephen G Stopped-flow, micro-fluidic device and method for the charge-based separation of complex analyte mixtures
WO2013123425A1 (en) 2012-02-15 2013-08-22 Bio-Rad Laboratories, Inc. Electronic control of ph and ionic strength
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
US9671368B2 (en) * 2013-05-10 2017-06-06 The Regents Of The University Of California Two-dimensional microfluidic devices and methods of using the same
KR20160029840A (ko) 2013-07-05 2016-03-15 라보라토이레 프란카이즈 듀 프락티온네먼트 에트 데스 바이오테크놀로지스 친화성 크로마토그래피 매트릭스
SG11201600221YA (en) 2013-07-12 2016-02-26 Genentech Inc Elucidation of ion exchange chromatography input optimization

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3539493A (en) * 1967-08-31 1970-11-10 Canal Ind Corp Apparatus for preparative electrophoresis on gel support media
US3844925A (en) * 1973-07-02 1974-10-29 Center For Blood Res Molecular fractionation
SE415731B (sv) * 1977-04-26 1980-10-27 Pharmacia Fine Chemicals Ab Vattenloslig amfolyt for separationsendamal samt sett att framstella densamma
GB2036081B (en) * 1977-06-15 1982-08-25 Nat Res Dev Membrane electrophoresis
EP0103965A2 (en) * 1982-08-20 1984-03-28 Imperial Chemical Industries Plc Electrofocusing apparatus
US5114555A (en) * 1988-01-05 1992-05-19 Monsanto Company Continuous isoelectric separation

Also Published As

Publication number Publication date
FI97893B (fi) 1996-11-29
FI97893C (fi) 1997-03-10
DE3876273D1 (de) 1993-01-14
DE3876273T2 (de) 1993-05-27
EP0287513B1 (en) 1992-12-02
DK191388D0 (da) 1988-04-08
DK191388A (da) 1988-10-12
FI881652A7 (fi) 1988-10-12
GR3007124T3 (cs) 1993-07-30
IE881067L (en) 1988-10-11
IE62803B1 (en) 1995-03-08
EP0287513A2 (en) 1988-10-19
US4971670A (en) 1990-11-20
JPH0781987B2 (ja) 1995-09-06
PT87210A (pt) 1989-05-12
FI881652A0 (fi) 1988-04-08
JPS63263457A (ja) 1988-10-31
CA1335805C (en) 1995-06-06
HK192495A (en) 1995-12-29
PT87210B (pt) 1993-11-30
US5082548A (en) 1992-01-21
EP0287513A3 (en) 1990-10-10
ES2037273T3 (es) 1993-06-16

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