DK167898B1 - Fremgangsmaade til forbedring af en antigenspecifik immunreaktion af dyreceller en vitro - Google Patents
Fremgangsmaade til forbedring af en antigenspecifik immunreaktion af dyreceller en vitro Download PDFInfo
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- DK167898B1 DK167898B1 DK334884A DK334884A DK167898B1 DK 167898 B1 DK167898 B1 DK 167898B1 DK 334884 A DK334884 A DK 334884A DK 334884 A DK334884 A DK 334884A DK 167898 B1 DK167898 B1 DK 167898B1
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US06/439,846 US4539205A (en) | 1982-11-09 | 1982-11-09 | Modulation of animal cellular responses with compositions containing 8-substituted guanine derivatives |
| US43984682 | 1982-11-09 | ||
| US54667983 | 1983-11-01 | ||
| US06/546,679 US4643992A (en) | 1982-11-09 | 1983-11-01 | Modulation of animal cellular responses with compositions containing 8-substituted guanine derivatives |
| US8301722 | 1983-11-07 | ||
| PCT/US1983/001722 WO1984001898A1 (en) | 1982-11-09 | 1983-11-07 | Modulation of animal cellular responses with compositions containng 8-substituted guanine derivatives |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DK334884D0 DK334884D0 (da) | 1984-07-06 |
| DK334884A DK334884A (da) | 1984-07-06 |
| DK167898B1 true DK167898B1 (da) | 1994-01-03 |
Family
ID=27032191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK334884A DK167898B1 (da) | 1982-11-09 | 1984-07-06 | Fremgangsmaade til forbedring af en antigenspecifik immunreaktion af dyreceller en vitro |
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|---|---|
| US (1) | US4643992A (cg-RX-API-DMAC10.html) |
| EP (1) | EP0112632B1 (cg-RX-API-DMAC10.html) |
| AU (1) | AU568325B2 (cg-RX-API-DMAC10.html) |
| CA (1) | CA1236401A (cg-RX-API-DMAC10.html) |
| DE (1) | DE3382156D1 (cg-RX-API-DMAC10.html) |
| DK (1) | DK167898B1 (cg-RX-API-DMAC10.html) |
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| IE (1) | IE56701B1 (cg-RX-API-DMAC10.html) |
| IL (1) | IL70164A (cg-RX-API-DMAC10.html) |
| NO (1) | NO174215C (cg-RX-API-DMAC10.html) |
| NZ (1) | NZ206208A (cg-RX-API-DMAC10.html) |
| PT (1) | PT77636B (cg-RX-API-DMAC10.html) |
| WO (1) | WO1984001898A1 (cg-RX-API-DMAC10.html) |
Families Citing this family (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4849411A (en) * | 1982-11-09 | 1989-07-18 | Scripps Clinic And Research Foundation | Modulation of animal cellular responses with compositions containing 8-substituted guanine derivatives |
| US5476659A (en) * | 1982-11-09 | 1995-12-19 | The Scripps Research Institute | Cancerous B cell treatment using substituted nucleoside derivatives |
| US4900675A (en) * | 1983-11-01 | 1990-02-13 | Scripps Clinic And Research Foundation | Modulation of animal cellular responses with compositions containing isoxanthopterin-8-(1'-β-aldoglycosidyl) derivatives |
| US5166141A (en) * | 1983-11-01 | 1992-11-24 | Scripps Clinic And Research Foundation | Immunostimulating 7-deaza-7-oxa- and 7-deaza-7-oxo-analogs of 8-substituted-guanine-9-(1'-beta-D-aldoglycosidyl) derivatives and methods of treating test animals |
| US5093318A (en) * | 1983-11-01 | 1992-03-03 | Scripps Clinic And Research Foundation | Immunostimulating guanosine derivatives and their pharmaceutical compositions |
| FR2560212B1 (fr) * | 1984-02-24 | 1989-12-29 | Unicet | Anticorps monoclonaux contre l'interferon a2 et hybridomes produisant de tels anticorps |
| US5011828A (en) * | 1985-11-15 | 1991-04-30 | Michael Goodman | Immunostimulating guanine derivatives, compositions and methods |
| ZA869297B (en) * | 1985-12-13 | 1987-11-25 | Scripps Clinic Res | Modulation of animal cellular responses with compositions containing isoxanthopterin-8-(1'-beta-aldoglycosidyl)derivatives |
| US6060459A (en) * | 1987-10-28 | 2000-05-09 | Pro-Neuron, Inc. | Enhancing blood cell count with oxypurine nucleosides |
| US4880784A (en) * | 1987-12-21 | 1989-11-14 | Brigham Young University | Antiviral methods utilizing ribofuranosylthiazolo[4,5-d]pyrimdine derivatives |
| US4902985A (en) * | 1988-02-25 | 1990-02-20 | Raytheon Company | Microwave reflectiion amplifiers having increased bandwidth |
| GB2216416B (en) * | 1988-03-11 | 1992-06-24 | Sandoz Ltd | Nucleobase source for the stimulation of the immune system |
| ZA893063B (en) * | 1988-04-27 | 1990-01-31 | Immunomedics Inc | Improved radiotherapy |
| WO1994011003A1 (en) * | 1992-11-13 | 1994-05-26 | The Scripps Research Institute | Cancerous b cell treatment using substituted nucleoside derivatives |
| US5786359A (en) * | 1994-05-27 | 1998-07-28 | The Scripps Research Institute | N9 alkyl or aralkyl derivatives of 7, 8-disubstituted guanines |
| US5441942A (en) * | 1994-05-27 | 1995-08-15 | The Scripps Research Institute | 2'3'-dideoxy-2',3'-didehydro-7,8-disubstituted guanosines and their immunostimulative effect |
| US6338963B1 (en) | 1994-07-25 | 2002-01-15 | Neotherapeutics, Inc. | Use of carbon monoxide dependent guanylyl cyclase modifiers to stimulate neuritogenesis |
| US20030100520A1 (en) * | 1997-01-21 | 2003-05-29 | Philip Needleman | Immunological process and constructs for increasing the hdl cholesterol concentration by dna vaccination |
| WO1999057120A1 (en) | 1998-05-04 | 1999-11-11 | Neotherapeutics, Inc. | Novel serotonin-like 9-substituted hypoxanthine and methods of use |
| US6562801B1 (en) * | 1999-04-15 | 2003-05-13 | Board Of Regents, The University Of Texas System | PpGpp and pppGpp as immunomodulatory agents |
| US6288069B1 (en) | 1999-11-16 | 2001-09-11 | Neotherapeutics, Inc. | Use of 9-substituted hypoxanthine derivatives to stimulate regeneration of nervous tissue |
| US6407237B1 (en) | 2001-02-21 | 2002-06-18 | Neotherapeutics, Inc. | Crystal forms of 9-substituted hypoxanthine derivatives |
| US6849735B1 (en) * | 2000-06-23 | 2005-02-01 | Merck Eprova Ag | Methods of synthesis for 9-substituted hypoxanthine derivatives |
| EP1334104A2 (en) | 2000-07-07 | 2003-08-13 | Neotherapeutics, Inc. | Methods for treatment of drug-induced peripheral neuropathy and related conditions |
| US20030138769A1 (en) * | 2000-08-16 | 2003-07-24 | Birkett Ashley J. | Immunogenic HBc chimer particles having enhanced stability |
| US6759427B2 (en) * | 2001-04-20 | 2004-07-06 | Spectrum Pharmaceuticals, Inc. | Synthesis and methods of use of tetrahydroindolone analogues and derivatives |
| EP1395262A4 (en) * | 2001-06-15 | 2006-04-12 | Ribapharm Corp | NUCLEOSIDE-VACCINE-ADJUVANTS |
| US20030055249A1 (en) * | 2001-07-17 | 2003-03-20 | Fick David B. | Synthesis and methods of use of pyrimidine analogues and derivatives |
| US7361352B2 (en) * | 2001-08-15 | 2008-04-22 | Acambis, Inc. | Influenza immunogen and vaccine |
| US20030175863A1 (en) * | 2001-08-15 | 2003-09-18 | Birkett Ashley J. | Influenza immunogen and vaccine |
| US7321033B2 (en) * | 2001-11-27 | 2008-01-22 | Anadys Pharmaceuticals, Inc. | 3-B-D-ribofuranosylthiazolo [4,5-d] pyrimidine nucleosides and uses thereof |
| US7351413B2 (en) * | 2002-02-21 | 2008-04-01 | Lorantis, Limited | Stabilized HBc chimer particles as immunogens for chronic hepatitis |
| CA2537450C (en) | 2003-09-05 | 2012-04-17 | Anadys Pharmaceuticals, Inc. | Administration of tlr7 ligands and prodrugs thereof for treatment of infection by hepatitis c virus |
| PT2561872E (pt) | 2004-12-17 | 2014-12-18 | Anadys Pharmaceuticals Inc | Compostos 3h-oxazolo[4,5-d]pirimidin-2-ona 3,5-di- -substituídos e 3,5,7-tri-substituídos e seus pró-fármacos |
| US7781581B2 (en) | 2005-11-21 | 2010-08-24 | Anadys Pharmaceuticals, Inc. | Process for the preparation of 5-amino-3H-thiazolo[4,5-d]pyrimidin-2-one |
| BRPI0713680A2 (pt) * | 2006-06-22 | 2012-10-23 | Anadys Pharmaceuticals Inc | composto, composição farmacêutica e métodos de modulação das atividades imuno da citocina, de tratamento de infecção de vìrus da hepatite c em paciente e de tratamento de desordem de proliferação relacionada em mamìfero carecido do mesmo |
| BRPI0714831A2 (pt) | 2006-07-18 | 2013-04-02 | Anadys Pharmaceuticals Inc | composto, composiÇço farmacÊutica e mÉtodos de modulaÇço de atividades imuno da citoquina em paciente, de tratamento de infecÇço de vÍrus c da hepatite em paciente e de desordem relacionada com proliferaÇço em mamÍfero necessitado do mesmo |
| CA2695399C (en) | 2007-08-02 | 2017-10-17 | Biondvax Pharmaceuticals Ltd. | Multimeric multiepitope influenza vaccines |
| AU2008331800A1 (en) | 2007-12-03 | 2009-06-11 | President And Fellows Of Harvard College | Chlamydia antigens |
| JP2012510289A (ja) | 2008-12-03 | 2012-05-10 | プロティア ワクチン テクノロジーズ リミテッド | グルタミルｔＲＮＡ合成酵素（ＧｔＳ）フラグメント |
| TW201639852A (zh) | 2008-12-09 | 2016-11-16 | 吉李德科學股份有限公司 | 用於製備可用作類鐸受體調節劑之化合物的中間體化合物 |
| WO2011067758A2 (en) | 2009-12-02 | 2011-06-09 | Protea Vaccine Technologies Ltd. | Immunogenic fragments and multimers from streptococcus pneumoniae proteins |
| AU2011360572B2 (en) | 2011-02-22 | 2017-03-02 | Biondvax Pharmaceuticals Ltd. | Multimeric multiepitope polypeptides in improved seasonal and pandemic influenza vaccines |
| WO2013150518A1 (en) | 2012-04-01 | 2013-10-10 | Rappaport Family Institute For Research In The Medical Sciences | Extracellular matrix metalloproteinase inducer (emmprin) peptides and binding antibodies |
| CA2944768C (en) | 2014-04-03 | 2022-08-30 | Biondvax Pharmaceuticals Ltd. | Compositions of multimeric-multiepitope influenza polypeptides and their production |
| NZ728072A (en) | 2014-07-11 | 2018-06-29 | Gilead Sciences Inc | Modulators of toll-like receptors for the treatment of hiv |
| CA2960436C (en) | 2014-09-16 | 2021-01-05 | Gilead Sciences, Inc. | Solid forms of a toll-like receptor modulator |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| GB1174077A (en) * | 1967-02-18 | 1969-12-10 | Ajinomoto Kk | Process for Preparing 8-Mercaptopurine Derivatives |
-
1983
- 1983-11-01 US US06/546,679 patent/US4643992A/en not_active Expired - Lifetime
- 1983-11-07 AU AU23302/84A patent/AU568325B2/en not_active Expired
- 1983-11-07 WO PCT/US1983/001722 patent/WO1984001898A1/en not_active Ceased
- 1983-11-08 DE DE8383306791T patent/DE3382156D1/de not_active Expired - Lifetime
- 1983-11-08 CA CA000440679A patent/CA1236401A/en not_active Expired
- 1983-11-08 IL IL70164A patent/IL70164A/xx not_active IP Right Cessation
- 1983-11-08 IE IE2605/83A patent/IE56701B1/en not_active IP Right Cessation
- 1983-11-08 ES ES527138A patent/ES8601301A1/es not_active Expired
- 1983-11-08 EP EP83306791A patent/EP0112632B1/en not_active Expired - Lifetime
- 1983-11-09 NZ NZ206208A patent/NZ206208A/en unknown
- 1983-11-09 PT PT77636A patent/PT77636B/pt unknown
- 1983-11-09 GR GR72936A patent/GR78733B/el unknown
-
1984
- 1984-07-06 DK DK334884A patent/DK167898B1/da not_active IP Right Cessation
- 1984-07-06 NO NO842765A patent/NO174215C/no not_active IP Right Cessation
- 1984-07-09 FI FI842748A patent/FI87733C/fi not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| DK334884D0 (da) | 1984-07-06 |
| WO1984001898A1 (en) | 1984-05-24 |
| IE832605L (en) | 1984-05-09 |
| IL70164A0 (en) | 1984-02-29 |
| DK334884A (da) | 1984-07-06 |
| US4643992A (en) | 1987-02-17 |
| NZ206208A (en) | 1987-03-31 |
| EP0112632B1 (en) | 1991-02-06 |
| FI842748A0 (fi) | 1984-07-09 |
| FI87733B (fi) | 1992-11-13 |
| IE56701B1 (en) | 1991-11-06 |
| EP0112632A3 (en) | 1985-01-23 |
| FI87733C (fi) | 1993-02-25 |
| IL70164A (en) | 1988-06-30 |
| DE3382156D1 (de) | 1991-03-14 |
| PT77636B (en) | 1986-03-19 |
| EP0112632A2 (en) | 1984-07-04 |
| NO174215B (no) | 1993-12-20 |
| AU568325B2 (en) | 1987-12-24 |
| GR78733B (cg-RX-API-DMAC10.html) | 1984-10-02 |
| NO842765L (no) | 1984-07-06 |
| CA1236401A (en) | 1988-05-10 |
| ES527138A0 (es) | 1985-11-01 |
| FI842748L (fi) | 1984-07-09 |
| PT77636A (en) | 1983-12-01 |
| ES8601301A1 (es) | 1985-11-01 |
| AU2330284A (en) | 1984-06-04 |
| NO174215C (no) | 1994-03-30 |
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| B1 | Patent granted (law 1993) | ||
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