DK1539948T3 - Forbedrede metoder til in vitro proteinsyntese - Google Patents

Forbedrede metoder til in vitro proteinsyntese

Info

Publication number
DK1539948T3
DK1539948T3 DK03788625.6T DK03788625T DK1539948T3 DK 1539948 T3 DK1539948 T3 DK 1539948T3 DK 03788625 T DK03788625 T DK 03788625T DK 1539948 T3 DK1539948 T3 DK 1539948T3
Authority
DK
Denmark
Prior art keywords
protein synthesis
improved methods
vitro protein
improved
vitro
Prior art date
Application number
DK03788625.6T
Other languages
English (en)
Inventor
James Robert Swartz
Michael Christopher Jewett
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Application granted granted Critical
Publication of DK1539948T3 publication Critical patent/DK1539948T3/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK03788625.6T 2002-08-19 2003-08-18 Forbedrede metoder til in vitro proteinsyntese DK1539948T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40459102P 2002-08-19 2002-08-19
PCT/US2003/025888 WO2004016778A1 (en) 2002-08-19 2003-08-18 Improved methods of in vitro protein synthesis

Publications (1)

Publication Number Publication Date
DK1539948T3 true DK1539948T3 (da) 2010-03-01

Family

ID=31888382

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03788625.6T DK1539948T3 (da) 2002-08-19 2003-08-18 Forbedrede metoder til in vitro proteinsyntese

Country Status (12)

Country Link
US (2) US7338789B2 (da)
EP (2) EP2108697A1 (da)
JP (2) JP5259046B2 (da)
AT (1) ATE445699T1 (da)
AU (1) AU2003259912B2 (da)
CA (1) CA2496437C (da)
DE (1) DE60329692D1 (da)
DK (1) DK1539948T3 (da)
ES (1) ES2334126T3 (da)
PT (1) PT1539948E (da)
SI (1) SI1539948T1 (da)
WO (1) WO2004016778A1 (da)

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US20110129438A1 (en) 2006-06-28 2011-06-02 James Robert Swartz Immunogenic protein constructs
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EP2035554B1 (en) * 2006-06-29 2013-04-24 The Board of Trustees of The Leland Stanford Junior University Cell-free synthesis of proteins containing unnatural amino acids
EP2118129A4 (en) * 2007-03-01 2010-04-28 Life Technologies Corp ISOLATED PHOSPHOLIPID PROTEIN PARTICLES
US20100168402A1 (en) * 2008-11-14 2010-07-01 Bundy Bradley C Direct Attachment of Polypeptides to Virus Like Particles
WO2010077806A1 (en) 2008-12-15 2010-07-08 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways
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US8956833B2 (en) 2010-05-07 2015-02-17 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through enzyme relocation
EP3219796B1 (en) 2010-08-31 2020-10-07 GreenLight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
EP2753702B1 (en) 2011-09-09 2021-12-15 GreenLight Biosciences, Inc. Cell-free preparation of carbapenems
US9296792B2 (en) 2012-05-23 2016-03-29 The Board Of Trustees Of The Leland Stanford Junior University Ordered flagellin array as an immunostimulant
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ES2611788T3 (es) 2012-06-26 2017-05-10 Sutro Biopharma, Inc. Proteínas de Fc modificadas que comprenden residuos de aminoácidos no naturales específicos del sitio, conjugados de las mismas, métodos para su preparación y métodos para su uso
SI3584255T1 (sl) 2012-08-31 2022-05-31 Sutro Biopharma, Inc Modificirane aminokisline, ki vsebujejo azido skupino
KR102018863B1 (ko) 2012-10-12 2019-09-05 서트로 바이오파마, 인크. 개선된 발현을 위해 박테리아 추출물 중 선택 단백질의 단백질분해 불활성화
EP2935598A1 (en) 2012-12-21 2015-10-28 Greenlight Biosciences Inc. Cell-free system for converting methane into fuel, pyruvate or isobutanol
CA2936092A1 (en) 2013-01-23 2014-07-31 The Board Of Trustees Of The Leland Stanford Junior University Stabilized hepatitis b core polypeptide
US9255255B2 (en) 2013-03-04 2016-02-09 The Board Of Trustees Of The Leland Stanford Junior University Synthesis of linear and branched polymers of polypeptides through direct conjugation
US9528137B2 (en) 2013-03-15 2016-12-27 Northwestern University Methods for cell-free protein synthesis
EP2989239B1 (en) 2013-04-25 2018-07-04 Sutro Biopharma, Inc. Selection of fab fragments using ribosomal display technology
WO2014176439A1 (en) 2013-04-25 2014-10-30 Sutro Biopharma, Inc. The use of lambda-gam protein in ribosomal display technology
WO2015006555A2 (en) 2013-07-10 2015-01-15 Sutro Biopharma, Inc. Antibodies comprising multiple site-specific non-natural amino acid residues, methods of their preparation and methods of their use
JP6483687B2 (ja) 2013-08-05 2019-03-13 グリーンライト バイオサイエンシーズ インコーポレーテッドGreenlight Biosciences,Inc. プロテアーゼ切断部位を有する操作されたタンパク質
US9840493B2 (en) 2013-10-11 2017-12-12 Sutro Biopharma, Inc. Modified amino acids comprising tetrazine functional groups, methods of preparation, and methods of their use
US9951392B2 (en) 2014-03-14 2018-04-24 Northwestern University Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis
WO2016004024A1 (en) 2014-07-02 2016-01-07 Sutro Biopharma, Inc. High growth capacity auxotrophic escherichia coli and methods of use
US10118950B2 (en) 2014-08-30 2018-11-06 Northwestern University Platforms for cell-free protein synthesis comprising extracts from genomically recoded E. coli strains having genetic knock-out mutations in release factor 1 (RF-1) and endA
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US9908064B2 (en) 2015-02-06 2018-03-06 Leidos, Inc. Portable fluidic platform for rapid cell-free production of protein biologics
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US11913052B2 (en) 2015-06-10 2024-02-27 Northwestern University Cell-free protein synthesis driven metabolic engineering
US10577632B2 (en) 2015-06-10 2020-03-03 Northwestern University Cell-free protein synthesis driven metabolic engineering for the production of 1-butanol
US10221401B2 (en) 2015-09-08 2019-03-05 The Board Of Trustees Of The Leland Stanford Junior University Oxygen tolerant hydrogenase by mutating electron supply pathway
KR20180104106A (ko) 2016-01-27 2018-09-19 서트로 바이오파마, 인크. anti-CD74 항체 접합체, anti-CD74 항체 접합체를 포함하는 조성물 및 anti-CD74 항체 접합체의 이용 방법
JP7011599B2 (ja) 2016-04-06 2022-02-10 グリーンライト バイオサイエンシーズ インコーポレーテッド リボ核酸の無細胞的生産
US10829795B2 (en) 2016-07-14 2020-11-10 Northwestern University Method for rapid in vitro synthesis of glycoproteins via recombinant production of N-glycosylated proteins in prokaryotic cell lysates
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US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
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US11898187B2 (en) 2017-08-15 2024-02-13 Northwestern University Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases
AR113764A1 (es) 2017-10-11 2020-06-10 Greenlight Biosciences Inc Métodos y composiciones para la producción de nucleósido trifosfato y ácido ribonucleico
US11319320B2 (en) 2017-11-06 2022-05-03 Snap Bio, Inc. PIM kinase inhibitor compositions, methods, and uses thereof
WO2019094859A1 (en) 2017-11-10 2019-05-16 Northwestern University Cell-free protein synthesis platform derived from cellular extracts of vibrio natriegens
CN109971783A (zh) * 2017-12-28 2019-07-05 康码(上海)生物科技有限公司 一种体外蛋白合成体系、试剂盒及其制备方法
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
US11725224B2 (en) 2018-04-16 2023-08-15 Northwestern University Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
WO2020010030A1 (en) 2018-07-03 2020-01-09 Leidos, Inc. Materials and methods for cell-free expression of vaccine epitope concatemers
AU2020328511A1 (en) 2019-08-09 2022-03-03 Nutcracker Therapeutics, Inc. Methods and apparatuses for manufacturing for removing material from a therapeutic composition
US20230095053A1 (en) 2020-03-03 2023-03-30 Sutro Biopharma, Inc. Antibodies comprising site-specific glutamine tags, methods of their preparation and methods of their use
KR20230069187A (ko) 2020-09-14 2023-05-18 서트로 바이오파마, 인크. 무세포 단백질 합성 시스템을 이용한 항체의 대량 생산 방법
WO2024035661A1 (en) 2022-08-08 2024-02-15 Sutro Biopharma, Inc. Spray dried extract

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US5478730A (en) * 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
US6337191B1 (en) 1999-03-22 2002-01-08 The Board Of Trustees Of The Leland Stanford Junior University Vitro protein synthesis using glycolytic intermediates as an energy source
US6548276B2 (en) 2000-09-06 2003-04-15 The Board Of Trustees Of The Leland Stanford Junior University Enhanced in vitro synthesis of active proteins containing disulfide bonds
AU2001217678A1 (en) * 2000-11-14 2002-05-27 The Board Of Trustees Of The Leland Stanford Junior University In vitro protein synthesis using glycolytic intermediates as an energy source

Also Published As

Publication number Publication date
US20040209321A1 (en) 2004-10-21
EP2108697A1 (en) 2009-10-14
PT1539948E (pt) 2010-01-20
US8357529B2 (en) 2013-01-22
CA2496437C (en) 2013-01-08
EP1539948A1 (en) 2005-06-15
ES2334126T3 (es) 2010-03-05
WO2004016778A1 (en) 2004-02-26
JP5259046B2 (ja) 2013-08-07
US20080138857A1 (en) 2008-06-12
CA2496437A1 (en) 2004-02-26
DE60329692D1 (de) 2009-11-26
SI1539948T1 (sl) 2010-02-26
JP2010279368A (ja) 2010-12-16
AU2003259912B2 (en) 2008-10-23
AU2003259912A1 (en) 2004-03-03
ATE445699T1 (de) 2009-10-15
EP1539948B1 (en) 2009-10-14
EP1539948A4 (en) 2006-05-17
US7338789B2 (en) 2008-03-04
JP2005536206A (ja) 2005-12-02

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