DK1539948T3 - Forbedrede metoder til in vitro proteinsyntese - Google Patents

Forbedrede metoder til in vitro proteinsyntese

Info

Publication number
DK1539948T3
DK1539948T3 DK03788625.6T DK03788625T DK1539948T3 DK 1539948 T3 DK1539948 T3 DK 1539948T3 DK 03788625 T DK03788625 T DK 03788625T DK 1539948 T3 DK1539948 T3 DK 1539948T3
Authority
DK
Denmark
Prior art keywords
protein synthesis
improved methods
vitro protein
improved
vitro
Prior art date
Application number
DK03788625.6T
Other languages
English (en)
Inventor
James Robert Swartz
Michael Christopher Jewett
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Application granted granted Critical
Publication of DK1539948T3 publication Critical patent/DK1539948T3/da

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK03788625.6T 2002-08-19 2003-08-18 Forbedrede metoder til in vitro proteinsyntese DK1539948T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40459102P 2002-08-19 2002-08-19
PCT/US2003/025888 WO2004016778A1 (en) 2002-08-19 2003-08-18 Improved methods of in vitro protein synthesis

Publications (1)

Publication Number Publication Date
DK1539948T3 true DK1539948T3 (da) 2010-03-01

Family

ID=31888382

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03788625.6T DK1539948T3 (da) 2002-08-19 2003-08-18 Forbedrede metoder til in vitro proteinsyntese

Country Status (12)

Country Link
US (2) US7338789B2 (da)
EP (2) EP1539948B1 (da)
JP (2) JP5259046B2 (da)
AT (1) ATE445699T1 (da)
AU (1) AU2003259912B2 (da)
CA (1) CA2496437C (da)
DE (1) DE60329692D1 (da)
DK (1) DK1539948T3 (da)
ES (1) ES2334126T3 (da)
PT (1) PT1539948E (da)
SI (1) SI1539948T1 (da)
WO (1) WO2004016778A1 (da)

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US20100063258A1 (en) * 2006-06-28 2010-03-11 The Board Of Trustees Of The Leland Stanford Junior University Fusion protein constructs
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EP2118129A4 (en) * 2007-03-01 2010-04-28 Life Technologies Corp ISOLATED PHOSPHOLIPID PROTEIN PARTICLES
US20100168402A1 (en) * 2008-11-14 2010-07-01 Bundy Bradley C Direct Attachment of Polypeptides to Virus Like Particles
WO2010077806A1 (en) 2008-12-15 2010-07-08 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways
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DK3219796T3 (da) 2010-08-31 2020-12-21 Greenlight Biosciences Inc Fremgangsmåder til kontrol af strømningen i metaboliske reaktionsveje ved hjælp af proteasemanipulation
JP2014526250A (ja) 2011-09-09 2014-10-06 グリーンライト バイオサイエンシーズ インコーポレーテッド カルバペネムの無細胞調製
WO2013177440A2 (en) 2012-05-23 2013-11-28 The Board Of Trustees Of The Leland Stanford Junior University Ordered flagellin array as an immunostimulant
EP2859017B1 (en) 2012-06-08 2019-02-20 Sutro Biopharma, Inc. Antibodies comprising site-specific non-natural amino acid residues, methods of their preparation and methods of their use
DK2863955T3 (da) 2012-06-26 2017-01-23 Sutro Biopharma Inc Modificerede fc-proteiner, der omfatter stedsspecifikke ikke-naturlige aminosyrerester, konjugater heraf, fremgangsmåder til fremstilling heraf og fremgangsmåder til anvendelse heraf
EP3584255B1 (en) 2012-08-31 2022-02-16 Sutro Biopharma, Inc. Modified amino acids comprising an azido group
US9650621B2 (en) * 2012-10-12 2017-05-16 Sutro Biopharma, Inc. Proteolytic inactivation of select proteins in bacterial extracts for improved expression
SG11201504930PA (en) 2012-12-21 2015-07-30 Greenlight Biosciences Inc Cell-free system for converting methane into fuel, pyruvate or isobutanol
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US9255255B2 (en) 2013-03-04 2016-02-09 The Board Of Trustees Of The Leland Stanford Junior University Synthesis of linear and branched polymers of polypeptides through direct conjugation
US9528137B2 (en) 2013-03-15 2016-12-27 Northwestern University Methods for cell-free protein synthesis
US20160069897A1 (en) 2013-04-25 2016-03-10 Suthro Biopharma, Inc. Selection of fab fragments using ribosomal display technology
WO2014176439A1 (en) 2013-04-25 2014-10-30 Sutro Biopharma, Inc. The use of lambda-gam protein in ribosomal display technology
US9764039B2 (en) 2013-07-10 2017-09-19 Sutro Biopharma, Inc. Antibodies comprising multiple site-specific non-natural amino acid residues, methods of their preparation and methods of their use
EP3030671B1 (en) 2013-08-05 2018-10-03 Greenlight Biosciences, Inc. Engineered proteins with a protease cleavage site
US9840493B2 (en) 2013-10-11 2017-12-12 Sutro Biopharma, Inc. Modified amino acids comprising tetrazine functional groups, methods of preparation, and methods of their use
US9951392B2 (en) 2014-03-14 2018-04-24 Northwestern University Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis
WO2016004024A1 (en) 2014-07-02 2016-01-07 Sutro Biopharma, Inc. High growth capacity auxotrophic escherichia coli and methods of use
US10118950B2 (en) 2014-08-30 2018-11-06 Northwestern University Platforms for cell-free protein synthesis comprising extracts from genomically recoded E. coli strains having genetic knock-out mutations in release factor 1 (RF-1) and endA
SG10202008302XA (en) 2014-12-04 2020-10-29 Celgene Corp Biomolecule conjugates
US9908064B2 (en) 2015-02-06 2018-03-06 Leidos, Inc. Portable fluidic platform for rapid cell-free production of protein biologics
CN107614682B (zh) 2015-03-30 2021-11-09 绿光生物科技股份有限公司 核糖核酸的无细胞生成
US11913052B2 (en) 2015-06-10 2024-02-27 Northwestern University Cell-free protein synthesis driven metabolic engineering
US10577632B2 (en) 2015-06-10 2020-03-03 Northwestern University Cell-free protein synthesis driven metabolic engineering for the production of 1-butanol
US10221401B2 (en) 2015-09-08 2019-03-05 The Board Of Trustees Of The Leland Stanford Junior University Oxygen tolerant hydrogenase by mutating electron supply pathway
RU2018130757A (ru) 2016-01-27 2020-02-27 Сутро Биофарма, Инк. Конъюгаты антитела к cd74, композиции, содержащие конъюгаты антитела к cd74, и способы использования конъюгатов антитела к cd74
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US10829795B2 (en) 2016-07-14 2020-11-10 Northwestern University Method for rapid in vitro synthesis of glycoproteins via recombinant production of N-glycosylated proteins in prokaryotic cell lysates
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
MA47223A (fr) 2016-12-30 2019-11-06 Sutrovax Inc Conjugués polypeptide-antigène avec des acides aminés non naturels
JP7186167B2 (ja) 2017-01-06 2022-12-08 グリーンライト バイオサイエンシーズ インコーポレーテッド 糖の無細胞的生産
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WO2019035916A1 (en) 2017-08-15 2019-02-21 Northwestern University DESIGN OF PROTEIN GLYCOSYLATION SITES BY RAPID EXPRESSION AND CHARACTERIZATION OF N-GLYCOSYLTRANSFERASES
MX2020003841A (es) 2017-10-11 2020-11-06 Greenlight Biosciences Inc Métodos y composiciones para la producción de nucleósido trifosfatos y ácidos ribonucleicos.
CN112739345A (zh) 2017-11-06 2021-04-30 斯奈普生物公司 Pim激酶抑制剂组合物,方法和其用途
US11673921B2 (en) 2017-11-10 2023-06-13 Northwestern University Cell-free protein synthesis platform derived from cellular extracts of Vibrio natriegens
CN109971783A (zh) * 2017-12-28 2019-07-05 康码(上海)生物科技有限公司 一种体外蛋白合成体系、试剂盒及其制备方法
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
US11725224B2 (en) 2018-04-16 2023-08-15 Northwestern University Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
CA3104859A1 (en) 2018-07-03 2020-01-09 Leidos, Inc. Materials and methods for cell-free expression of vaccine epitope concatemers
WO2021030271A2 (en) 2019-08-09 2021-02-18 Nutcracker Therapeutics, Inc. Methods and apparatuses for manufacturing for removing material from a therapeutic composition
US20230095053A1 (en) 2020-03-03 2023-03-30 Sutro Biopharma, Inc. Antibodies comprising site-specific glutamine tags, methods of their preparation and methods of their use
KR20230069187A (ko) 2020-09-14 2023-05-18 서트로 바이오파마, 인크. 무세포 단백질 합성 시스템을 이용한 항체의 대량 생산 방법
US20240043895A1 (en) 2022-08-08 2024-02-08 Sutro Biopharma, Inc. Spray dried extract

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US5478730A (en) * 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
US6337191B1 (en) * 1999-03-22 2002-01-08 The Board Of Trustees Of The Leland Stanford Junior University Vitro protein synthesis using glycolytic intermediates as an energy source
US6548276B2 (en) 2000-09-06 2003-04-15 The Board Of Trustees Of The Leland Stanford Junior University Enhanced in vitro synthesis of active proteins containing disulfide bonds
AU2001217678A1 (en) * 2000-11-14 2002-05-27 The Board Of Trustees Of The Leland Stanford Junior University In vitro protein synthesis using glycolytic intermediates as an energy source

Also Published As

Publication number Publication date
US8357529B2 (en) 2013-01-22
US7338789B2 (en) 2008-03-04
EP1539948B1 (en) 2009-10-14
AU2003259912A1 (en) 2004-03-03
ES2334126T3 (es) 2010-03-05
EP1539948A4 (en) 2006-05-17
AU2003259912B2 (en) 2008-10-23
JP2010279368A (ja) 2010-12-16
PT1539948E (pt) 2010-01-20
JP2005536206A (ja) 2005-12-02
WO2004016778A1 (en) 2004-02-26
US20040209321A1 (en) 2004-10-21
ATE445699T1 (de) 2009-10-15
DE60329692D1 (de) 2009-11-26
EP1539948A1 (en) 2005-06-15
SI1539948T1 (sl) 2010-02-26
JP5259046B2 (ja) 2013-08-07
CA2496437A1 (en) 2004-02-26
CA2496437C (en) 2013-01-08
EP2108697A1 (en) 2009-10-14
US20080138857A1 (en) 2008-06-12

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