DK1456394T4 - Fremgangsmåder til anvendelse forbedrede polymeraser - Google Patents

Fremgangsmåder til anvendelse forbedrede polymeraser

Info

Publication number
DK1456394T4
DK1456394T4 DK02786844.7T DK02786844T DK1456394T4 DK 1456394 T4 DK1456394 T4 DK 1456394T4 DK 02786844 T DK02786844 T DK 02786844T DK 1456394 T4 DK1456394 T4 DK 1456394T4
Authority
DK
Denmark
Prior art keywords
methods
use improved
improved polymerases
polymerases
polymerase
Prior art date
Application number
DK02786844.7T
Other languages
Danish (da)
English (en)
Other versions
DK1456394T3 (da
Inventor
Yan Wang
Horn Peter Vander
Lei Xi
Original Assignee
Bio Rad Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23304983&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1456394(T4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bio Rad Laboratories filed Critical Bio Rad Laboratories
Publication of DK1456394T3 publication Critical patent/DK1456394T3/da
Application granted granted Critical
Publication of DK1456394T4 publication Critical patent/DK1456394T4/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
DK02786844.7T 2001-11-28 2002-11-27 Fremgangsmåder til anvendelse forbedrede polymeraser DK1456394T4 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33396601P 2001-11-28 2001-11-28
PCT/US2002/038441 WO2003046149A2 (en) 2001-11-28 2002-11-27 Methods of using improved polymerases

Publications (2)

Publication Number Publication Date
DK1456394T3 DK1456394T3 (da) 2008-11-24
DK1456394T4 true DK1456394T4 (da) 2011-08-15

Family

ID=23304983

Family Applications (1)

Application Number Title Priority Date Filing Date
DK02786844.7T DK1456394T4 (da) 2001-11-28 2002-11-27 Fremgangsmåder til anvendelse forbedrede polymeraser

Country Status (10)

Country Link
US (4) US8232078B2 (https=)
EP (1) EP1456394B2 (https=)
JP (1) JP4689163B2 (https=)
AT (1) ATE406456T1 (https=)
AU (1) AU2002351198B2 (https=)
CA (1) CA2468838A1 (https=)
DE (1) DE60228618D1 (https=)
DK (1) DK1456394T4 (https=)
ES (1) ES2311638T5 (https=)
WO (1) WO2003046149A2 (https=)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004227353B2 (en) 2003-04-01 2010-02-25 Luminex Corporation Polymerase inhibitor and method of using same
EP1706502A4 (en) * 2003-11-26 2007-05-23 Eppendorf Ag METHODS AND COMPOSITIONS FOR IN VITRO AMPLIFICATION OF EXTRACHROMOSOMIC NUCLEIC ACID
US20060228726A1 (en) * 2005-01-06 2006-10-12 Martin Patrick K Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
US8349600B2 (en) 2005-01-06 2013-01-08 Applied Biosystems, Llc Polypeptides having nucleic acid binding activity
WO2007050125A2 (en) * 2005-05-27 2007-05-03 William Marsh Rice University High processivity polymerases
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
JP4899618B2 (ja) * 2006-05-01 2012-03-21 ソニー株式会社 二本鎖核酸検出方法、ハイブリダイゼーション検出方法
KR100825279B1 (ko) * 2006-11-30 2008-04-25 한국해양연구원 Ｄｎα 중합효소 활성 증가 단백질 및 이를 암호화 하는유전자
CA2706444C (en) * 2007-11-27 2017-06-06 Bio-Rad Laboratories, Inc. Reduced inhibition of one-step rt-pcr
WO2009087394A1 (en) * 2008-01-11 2009-07-16 Genesys Ltd Cren7 chimeric protein
GB0803628D0 (en) 2008-02-28 2008-04-02 Genesys Ltd Enzyme
WO2010062777A2 (en) 2008-11-03 2010-06-03 Kapabiosystems Modified type a dna polymerases
JP5818688B2 (ja) 2009-01-08 2015-11-18 バイオ−ラッド ラボラトリーズ インコーポレーティッド 核酸増幅反応の効率を改善するための方法および組成物
EP2393933A4 (en) * 2009-02-04 2013-05-01 Lucigen Corp RNA AND DNA COPIERING ENZYMES
US9617588B2 (en) 2010-10-05 2017-04-11 Thermo Fisher Scientific Baltics Uab Enzyme mixture
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
CN103703141B (zh) 2011-04-08 2016-08-17 伯乐实验室公司 具有降低的非特异性活性的pcr反应混合物
JP6060447B2 (ja) * 2011-04-08 2017-01-18 バイオ−ラッド ラボラトリーズ インコーポレーティッド 非特異的活性が低下したＳｓｏ７ポリメラーゼコンジュゲート
US8470573B2 (en) 2011-06-21 2013-06-25 Bio-Rad Laboratories, Inc. Hybrid polymerases having the ability to produce long amplicons
US10023856B2 (en) * 2013-09-25 2018-07-17 Thermo Fisher Scientific Baltics Uab Enzyme composition for DNA end repair, adenylation, phosphorylation
WO2016028671A1 (en) 2014-08-19 2016-02-25 Bio-Rad Laboratories, Inc. Rna amplification methods
EP3778891A1 (en) * 2014-08-27 2021-02-17 New England Biolabs, Inc. Synthon formation
US9963687B2 (en) * 2014-08-27 2018-05-08 New England Biolabs, Inc. Fusion polymerase and method for using the same
WO2016134059A1 (en) 2015-02-17 2016-08-25 Bio-Rad Laboratories, Inc. Small nucleic acid quantification using split cycle amplification
US20200392562A1 (en) 2017-12-22 2020-12-17 Thermo Fisher Scientific Baltics Uab Polymerase chain reaction composition comprising amines
CN116200364A (zh) * 2019-03-10 2023-06-02 武汉爱博泰克生物科技有限公司 用于在提高的盐浓度或体液中扩增的突变型Taq聚合酶
US11613738B2 (en) 2019-05-14 2023-03-28 Abclonal Science, Inc. Mutant Taq polymerase resistant to inhibition of amplification in the presence of cyanine dye
US20250145989A1 (en) * 2022-01-26 2025-05-08 Seegene, Inc. Method for detecting target nucleic acids in sample

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512482A (en) * 1990-04-26 1996-04-30 Calgene, Inc. Plant thioesterases
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US5972603A (en) * 1996-02-09 1999-10-26 President And Fellows Of Harvard College DNA polymerase with modified processivity
US6228628B1 (en) * 1997-07-09 2001-05-08 Roche Molecular Systems Mutant chimeric DNA polymerase
DE19840771A1 (de) 1998-08-06 2000-02-10 Lion Bioscience Ag Thermostabiler in vitro-Komplex mit Polymeraseaktivität
DE19937230A1 (de) 1999-08-06 2001-02-08 Lion Bioscience Ag Chimäre Proteine
US6627424B1 (en) * 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes

Also Published As

Publication number Publication date
CA2468838A1 (en) 2003-06-05
AU2002351198A1 (en) 2003-06-10
EP1456394B2 (en) 2011-04-27
US9708598B2 (en) 2017-07-18
AU2002351198B2 (en) 2008-08-14
DE60228618D1 (de) 2008-10-09
US20120295270A1 (en) 2012-11-22
WO2003046149A3 (en) 2004-02-19
DK1456394T3 (da) 2008-11-24
US9139873B2 (en) 2015-09-22
US20140220577A1 (en) 2014-08-07
ATE406456T1 (de) 2008-09-15
WO2003046149A2 (en) 2003-06-05
US20040002076A1 (en) 2004-01-01
JP4689163B2 (ja) 2011-05-25
JP2005510235A (ja) 2005-04-21
ES2311638T3 (es) 2009-02-16
US8232078B2 (en) 2012-07-31
EP1456394A2 (en) 2004-09-15
US20160060616A1 (en) 2016-03-03
ES2311638T5 (es) 2011-09-08
US8476045B2 (en) 2013-07-02
EP1456394B1 (en) 2008-08-27
EP1456394A4 (en) 2005-12-28

Similar Documents

Publication Publication Date Title
DK1456394T3 (da) Fremgangsmåder til anvendelse forbedrede polymeraser
DE60235491D1 (de) Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur
ATE368104T1 (de) Verbesserte nukleinsäure-modifizierende enzyme
Washington et al. Efficient and error-free replication past a minor-groove DNA adduct by the sequential action of human DNA polymerases ι and κ
ATE557083T1 (de) Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität
WO2005098042A3 (en) Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity
EP4328303A3 (en) Novel use
WO2005005667A3 (en) 2'-terminator nucleotide-related methods and systems
ATE432365T1 (de) Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
WO2005083068A3 (en) Methods for obtaining thermostable enzymes, dna polymerase i variants from thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications to the same
EP1091002A3 (en) Sequential activation of one or more enzymatic activities within a thermocycling reaction for synthesizing DNA molecules
DE60122962D1 (de) Reverse Transkription bei hoher Temperatur durch die Verwendung von mutierten DNA Polymerasen
DE60133992D1 (de) Thermostabile chimäre nucleinsäurepolymerasen und ihre verwendungen
NO20012561D0 (no) Termostabile enzymer som fremmer fideliteten av termostabile DNA-polymeraser for forbedring av nukleinsyresyntese ogamplifikasjon in vitro
WO2001064838A3 (en) Thermophilic dna polymerases from thermoactinomyces vulgaris
Asagoshi et al. Comparison of substrate specificities of Escherichia coli endonuclease III and its mouse homologue (mNTH1) using defined oligonucleotide substrates
ATE330031T1 (de) Reversibel inhibierende sonden
Huang et al. Role of Calf RTH-1 Nuclease in Removal of 5 ‘-Ribonucleotides during Okazaki Fragment Processing
US20160230223A1 (en) Method Of Amplifying Nucleic Acid Sequences
LT2017523A (lt) N4-modifikuoti citidino nukleotidai ir jų panaudojimas
WO2004090153A3 (en) Polymerase inhibitor and method of using same
Kamiya et al. Two DNA polymerases of Escherichia coli display distinct misinsertion specificities for 2-hydroxy-dATP during DNA synthesis
ATE455866T1 (de) Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität
Hwang et al. Role of base stacking and sequence context in the inhibition of yeast DNA polymerase η by pyrene nucleotide
Gangurde et al. Participation of active-site carboxylates of Escherichia coli DNA polymerase I (Klenow fragment) in the formation of a prepolymerase ternary complex