DK1441591T3 - Sammensætninger og fremgangsmåder til priming af monocytiske dendritiske celler og T-celler for TH-1-respons - Google Patents
Sammensætninger og fremgangsmåder til priming af monocytiske dendritiske celler og T-celler for TH-1-respons Download PDFInfo
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Claims (10)
1. En ex vivo- eller in vitro-metode til frembringelse af en moden dendritisk cellepopulation, der frembringer et forhøjet forhold mellem Interleukin 12 og Interleukin 10, hvor det forhøjede forhold mellem Interleukin 12 og Interleukin 10 er forhøjet i forhold til en umoden dendritisk cellepopulation, der ikke er bragt i kontakt med Bacillus-Calmette-Guerin (BCG) og Interferon y (IFNy) under modning, der omfatter: kontakt gav umodne dendritiske celler en effektiv mængde Bacillus-Calmette-Guerin (BCG) bestående af 105 til 107 cfu pr. ml vævskulturmedier og 100 til 1000 enheder Interferon gamma (IFNy) pr. ml vævskulturmedier under kulturbetingelser, der er egnet til modning af de umodne dendritiske celler for at danne den modne dendritiske cellepopulation.
2. En ex vivo- eller in vitro-metode til frembringelse af en moden dendritisk cellepopulation, hvori den modne dendritiske cellepopulation frembringer en type 1-immunresponse, der omfatter: kontakt gav umodne dendritiske celler en effektiv mængde Bacillus-Calmette-Guerin (BCG) bestående af 105 til 107 cfu pr. ml vævskulturmedier og 100 til 1000 enheder Interferon gamma (IFNy) pr. ml vævskulturmedier under kulturbetingelser, der er egnet til modning af de umodne dendritiske celler for at danne den modne dendritiske cellepopulation.
3. En ex vivo- eller in vitro-metode til frembringelse af aktiverede T-celler, der omfatter: kontakt gav umodne dendritiske celler et forudbestemt antigen; og kontakt gav de umodne dendritiske celler en mængde Bacillus-Calmette-Guerin (BCG), bestående af 105 til 107 cfu pr. ml vævskulturmedier og 100 til 1000 enheder Interferon gamma (IFNy) pr. ml vævskulturmedier under kulturbetingelser, der er egnet til modning af de umodne dendritiske celler for at danne den modne dendritiske cellepopulation. hvori den modne dendritiske cellepopulation frembringer et forhøjet forhold mellem Interleukin 12 og Interleukin 10, og kontakt mellem de modne dendritiske celler og naive T-celler for at danne aktiverede T-celler til frembringelse af IFNy, og hvori den aktiverede T-cellepopulation frembringer en type 1-immunrespons.
4. Metoden i ifølge ethvert af kravene 1 til 3, hvori de modne dendritiske celler frembringer et forhold mellem IL-12 og IL-10 på mindst ca. 1:1, mindst ca. 10:1 eller mindst ca. 100: 1.
5. Metoden ifølge ethvert af kravene 1 til 3, der desuden omfatter: dyrkning af isolerede monocytiske dendritiske celleprækursorer ved tilstedeværelse af en monocytisk dendritisk celledifferentierende agent for at danne de umodne dendritiske celler.
6. Metoden i krav 5, hvori den differentierende agent er GM-CSF, Interleukin 4, en kombination af GM-CSF og Interleukin 4 eller Interleukin 13.
7. Metoden i krav 5, hvori de monocytiske dendritiske celleprækursorer er humane monocytiske dendritiske celleprækursorer.
8. Metoden i krav 3, hvori et forudbestemt antigen er et tumorspecifikt antigen, et tumorassocieret antigen, et viralt antigen, et bakterielt antigen, tumorceller, bakterieceller, rekombinante celler, der udtrykker et antigen, et cellelysat, et membranpræparat, et rekombinant produceret antigen, et peptidantigen eller et isoleret antigen.
9. Metoden i ifølge ethvert af kravene 3 til 8, hvori de umodne dendritiske celler bringes i samtidig kontakt med det forudbestemte antigen, BCG og IFNy eller bringes i kontakt med det forudbestemte antigen, før de bringes i kontakt med BCG og IFNy.
10. Metoden i ifølge ethvert af kravene 3 og 8 til 9, hvori de umodne dendritiske celler og T-cellerne er autologe i forhold til hinanden.
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| US31759201P | 2001-09-06 | 2001-09-06 | |
| PCT/US2002/028620 WO2003022215A2 (en) | 2001-09-06 | 2002-09-06 | Compositions and methods for priming monocytic dendritic cells and t cells for th-1 response |
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| DK10181985.2T DK2322603T3 (da) | 2001-09-06 | 2002-09-06 | Sammensætninger og fremgangsmåder til aktivering af monocytiske dendritiske celler og t-celler for th1-respons |
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| AT412145B (de) * | 2002-09-13 | 2004-10-25 | Forsch Krebskranke Kinder | Verfahren zur herstellung eines zellulären immuntherapeutikums auf basis von il-12-freisetzenden dendritischen zellen |
| US20040057935A1 (en) * | 2002-09-20 | 2004-03-25 | Cedars-Sinai Medical Center | Intratumoral delivery of dendritic cells |
| RU2348418C2 (ru) * | 2002-12-06 | 2009-03-10 | Норсуэст Байотерапьютикс, Инк. | Введение дендритных клеток, подвергнутых частичному созреванию in vitro, для лечения опухолей |
| ATE420946T1 (de) * | 2003-02-27 | 2009-01-15 | Northwest Biotherapeutics Inc | Erzeugung dendritischer zellen aus monozytischen dendritischen vorläuferzellen mit gm-csf ohne anwesenheit zusätzlicher zytokine |
| EP1484064A1 (en) | 2003-06-04 | 2004-12-08 | Gesellschaft für Biotechnologische Forschung | Therapeutical composition containing dentritic cells and use thereof |
| JP2007507543A (ja) * | 2003-10-06 | 2007-03-29 | セダーズ−シナイ メディカル センター | 樹状細胞治療によって誘導されるｔ細胞アネルギーを予防するためのｃｏｘ−２インヒビターの使用 |
| WO2005043155A1 (en) * | 2003-10-21 | 2005-05-12 | Cedars-Sinai Medical Center | System and method for the treatment of cancer, including cancers of the central nervous system |
| AU2004286144A1 (en) * | 2003-11-04 | 2005-05-12 | Dnavec Research Inc. | Method for producing gene transferred dendritic cells |
| JP5296983B2 (ja) | 2004-06-24 | 2013-09-25 | 株式会社ディナベック研究所 | Ｒｎａウイルスが導入された樹状細胞を含む抗癌剤 |
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