DK1058738T3 - Fremgangsmåde til screening, baseret på fluorescens-polarisering - Google Patents
Fremgangsmåde til screening, baseret på fluorescens-polariseringInfo
- Publication number
- DK1058738T3 DK1058738T3 DK99906092T DK99906092T DK1058738T3 DK 1058738 T3 DK1058738 T3 DK 1058738T3 DK 99906092 T DK99906092 T DK 99906092T DK 99906092 T DK99906092 T DK 99906092T DK 1058738 T3 DK1058738 T3 DK 1058738T3
- Authority
- DK
- Denmark
- Prior art keywords
- fluorescence polarization
- screening based
- transferase
- screening
- determination
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6445—Measuring fluorescence polarisation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/91045—Acyltransferases (2.3)
- G01N2333/91074—Aminoacyltransferases (general) (2.3.2)
- G01N2333/9108—Aminoacyltransferases (general) (2.3.2) with definite EC number (2.3.2.-)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
- G01N2333/918—Carboxylic ester hydrolases (3.1.1)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK30898 | 1998-03-06 | ||
| PCT/DK1999/000112 WO1999045143A2 (en) | 1998-03-06 | 1999-03-05 | A fluorescence polarization screening method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1058738T3 true DK1058738T3 (da) | 2002-03-11 |
Family
ID=8092089
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK99906092T DK1058738T3 (da) | 1998-03-06 | 1999-03-05 | Fremgangsmåde til screening, baseret på fluorescens-polarisering |
Country Status (11)
| Country | Link |
|---|---|
| EP (2) | EP1058738B1 (de) |
| JP (1) | JP2002505114A (de) |
| CN (2) | CN1560609A (de) |
| AT (1) | ATE208828T1 (de) |
| AU (1) | AU2612299A (de) |
| CA (1) | CA2322488A1 (de) |
| DE (1) | DE69900465T2 (de) |
| DK (1) | DK1058738T3 (de) |
| ES (1) | ES2168848T3 (de) |
| PT (1) | PT1058738E (de) |
| WO (1) | WO1999045143A2 (de) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69824895T2 (de) * | 1998-04-15 | 2005-06-30 | Hidaka, Hiroyoshi, Nagoya | Verfahren zur bestimmung des gens eines zielproteins mit hilfe eines medikamentes |
| ATE285478T1 (de) * | 1999-10-01 | 2005-01-15 | Novozymes As | Zellulosefilme zur durchfuehrung von screening- verfahren |
| US6426189B1 (en) | 1999-10-01 | 2002-07-30 | Novozymes A/S | Cellulose films for screening |
| WO2001032858A1 (en) * | 1999-11-05 | 2001-05-10 | Novozymes A/S | A high throughput screening (hts) method |
| AU2002309989A1 (en) | 2001-05-18 | 2002-12-03 | Rigel Pharmaceuticals, Incorporated | Directed evolution of protein in mammalian cells |
| CN1938591B (zh) * | 2004-03-18 | 2011-11-30 | 特兰斯泰克制药公司 | 荧光偏振分析 |
| FR2897164B1 (fr) | 2006-02-09 | 2008-03-14 | Commissariat Energie Atomique | Realisation de cavites pouvant etre remplies par un materiau fluidique dans un compose microtechnologique optique |
| FR2929715B1 (fr) | 2008-04-04 | 2010-09-10 | Commissariat Energie Atomique | Procede de realisation de cavites microniques ou submicroniques |
| FR2933205B1 (fr) | 2008-06-27 | 2010-08-13 | Commissariat Energie Atomique | Procede ameliore de realisation d'enceintes remplies de liquide et fermees par une membrane |
| FR2938834B1 (fr) | 2008-11-27 | 2011-03-04 | Commissariat Energie Atomique | Procede de realisation d'une matrice de retention et comprenant un liquide fonctionnel |
| FR2963112B1 (fr) | 2010-07-21 | 2013-02-15 | Commissariat Energie Atomique | Microstructure a parois a propriete optique determinee et procede de realisation de microstructures |
| WO2014067933A1 (en) | 2012-10-31 | 2014-05-08 | C-Lecta Gmbh | Bioactive carrier preparation for enhanced safety in care products and food |
| KR101494881B1 (ko) | 2013-05-06 | 2015-02-24 | 한국과학기술연구원 | 설퍼 옥시도리덕타아제 결합 형광 화합물을 이용한 바실러스 서브틸리스 검출 방법 |
| CN105866316B (zh) * | 2016-06-24 | 2018-03-30 | 曲阜师范大学 | 一种同时检测食品中氨基酸和生物胺的分析方法 |
| CN110954517A (zh) * | 2019-12-11 | 2020-04-03 | 西北大学 | 还原性糖的一步荧光衍生方法及其应用 |
| CN113514445B (zh) * | 2021-08-05 | 2023-12-26 | 荆州市第一人民医院（荆州市肿瘤医院、长江大学附属第一医院） | 一种基于荧光偏振的去泛素化酶活性检测方法 |
| CN114166807B (zh) * | 2021-11-22 | 2023-12-12 | 中国农业大学 | 用于检测多粘菌素的荧光偏振免疫分析方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3686071T2 (de) * | 1985-02-25 | 1993-01-07 | Abbott Lab | Polarisierter fluoreszenztest von makromolekularen hydrolasen. |
| MX9206979A (es) * | 1991-12-04 | 1993-07-01 | Novo Nordisk As | Metodo de clonacion de proteinas en levaduras |
| JPH0723800A (ja) * | 1993-07-08 | 1995-01-27 | Tanabe Seiyaku Co Ltd | 核酸の検出方法 |
| GB9519393D0 (en) * | 1995-09-22 | 1995-11-22 | Univ Edinburgh | Novel compounds and assay method |
-
1999
- 1999-03-05 AU AU26122/99A patent/AU2612299A/en not_active Abandoned
- 1999-03-05 EP EP99906092A patent/EP1058738B1/de not_active Expired - Lifetime
- 1999-03-05 PT PT99906092T patent/PT1058738E/pt unknown
- 1999-03-05 CA CA002322488A patent/CA2322488A1/en not_active Abandoned
- 1999-03-05 WO PCT/DK1999/000112 patent/WO1999045143A2/en active IP Right Grant
- 1999-03-05 DK DK99906092T patent/DK1058738T3/da active
- 1999-03-05 CN CNA2004100586739A patent/CN1560609A/zh active Pending
- 1999-03-05 AT AT99906092T patent/ATE208828T1/de active
- 1999-03-05 JP JP2000534674A patent/JP2002505114A/ja active Pending
- 1999-03-05 CN CNB998051519A patent/CN1167810C/zh not_active Expired - Fee Related
- 1999-03-05 ES ES99906092T patent/ES2168848T3/es not_active Expired - Lifetime
- 1999-03-05 DE DE69900465T patent/DE69900465T2/de not_active Expired - Lifetime
- 1999-03-05 EP EP01118580A patent/EP1156122A3/de not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| ATE208828T1 (de) | 2001-11-15 |
| DE69900465D1 (de) | 2001-12-20 |
| JP2002505114A (ja) | 2002-02-19 |
| DE69900465T2 (de) | 2002-09-05 |
| CN1297487A (zh) | 2001-05-30 |
| EP1156122A2 (de) | 2001-11-21 |
| AU2612299A (en) | 1999-09-20 |
| WO1999045143A3 (en) | 1999-10-21 |
| EP1156122A3 (de) | 2005-02-09 |
| CN1167810C (zh) | 2004-09-22 |
| CN1560609A (zh) | 2005-01-05 |
| EP1058738B1 (de) | 2001-11-14 |
| WO1999045143A2 (en) | 1999-09-10 |
| ES2168848T3 (es) | 2002-06-16 |
| PT1058738E (pt) | 2002-05-31 |
| EP1058738A2 (de) | 2000-12-13 |
| CA2322488A1 (en) | 1999-09-10 |
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