ES2168848T3 - Metodo de seleccion por polarizacion de fluorescencia. - Google Patents
Metodo de seleccion por polarizacion de fluorescencia.Info
- Publication number
- ES2168848T3 ES2168848T3 ES99906092T ES99906092T ES2168848T3 ES 2168848 T3 ES2168848 T3 ES 2168848T3 ES 99906092 T ES99906092 T ES 99906092T ES 99906092 T ES99906092 T ES 99906092T ES 2168848 T3 ES2168848 T3 ES 2168848T3
- Authority
- ES
- Spain
- Prior art keywords
- fluorescence polarization
- selection
- transferase
- determination
- compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6445—Measuring fluorescence polarisation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/91045—Acyltransferases (2.3)
- G01N2333/91074—Aminoacyltransferases (general) (2.3.2)
- G01N2333/9108—Aminoacyltransferases (general) (2.3.2) with definite EC number (2.3.2.-)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
- G01N2333/918—Carboxylic ester hydrolases (3.1.1)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Método para seleccionar una secuencia nucleótida que codifica un compuesto biológico que comprende: a) expresar la secuencia nucleótida en un sistema de expresión para producir el compuesto biológico, b) poner en contacto el compuesto biológico con una sustancia fluorescente capaz de reaccionar ante el compuesto biológico para dejar que la reacción tenga lugar, c) medir la polarización de fluorescencia de la sustancia fluorescente, y d) seleccionar los sistemas de expresión en los que haya ocurrido un cambio en la polarización de fluorescencia.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK30898 | 1998-03-06 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2168848T3 true ES2168848T3 (es) | 2002-06-16 |
Family
ID=8092089
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES99906092T Expired - Lifetime ES2168848T3 (es) | 1998-03-06 | 1999-03-05 | Metodo de seleccion por polarizacion de fluorescencia. |
Country Status (11)
Country | Link |
---|---|
EP (2) | EP1058738B1 (es) |
JP (1) | JP2002505114A (es) |
CN (2) | CN1560609A (es) |
AT (1) | ATE208828T1 (es) |
AU (1) | AU2612299A (es) |
CA (1) | CA2322488A1 (es) |
DE (1) | DE69900465T2 (es) |
DK (1) | DK1058738T3 (es) |
ES (1) | ES2168848T3 (es) |
PT (1) | PT1058738E (es) |
WO (1) | WO1999045143A2 (es) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69824895T2 (de) * | 1998-04-15 | 2005-06-30 | Hidaka, Hiroyoshi, Nagoya | Verfahren zur bestimmung des gens eines zielproteins mit hilfe eines medikamentes |
ATE285478T1 (de) * | 1999-10-01 | 2005-01-15 | Novozymes As | Zellulosefilme zur durchfuehrung von screening- verfahren |
US6426189B1 (en) | 1999-10-01 | 2002-07-30 | Novozymes A/S | Cellulose films for screening |
WO2001032858A1 (en) * | 1999-11-05 | 2001-05-10 | Novozymes A/S | A high throughput screening (hts) method |
AU2002309989A1 (en) | 2001-05-18 | 2002-12-03 | Rigel Pharmaceuticals, Incorporated | Directed evolution of protein in mammalian cells |
CN1938591B (zh) * | 2004-03-18 | 2011-11-30 | 特兰斯泰克制药公司 | 荧光偏振分析 |
FR2897164B1 (fr) | 2006-02-09 | 2008-03-14 | Commissariat Energie Atomique | Realisation de cavites pouvant etre remplies par un materiau fluidique dans un compose microtechnologique optique |
FR2929715B1 (fr) | 2008-04-04 | 2010-09-10 | Commissariat Energie Atomique | Procede de realisation de cavites microniques ou submicroniques |
FR2933205B1 (fr) | 2008-06-27 | 2010-08-13 | Commissariat Energie Atomique | Procede ameliore de realisation d'enceintes remplies de liquide et fermees par une membrane |
FR2938834B1 (fr) | 2008-11-27 | 2011-03-04 | Commissariat Energie Atomique | Procede de realisation d'une matrice de retention et comprenant un liquide fonctionnel |
FR2963112B1 (fr) | 2010-07-21 | 2013-02-15 | Commissariat Energie Atomique | Microstructure a parois a propriete optique determinee et procede de realisation de microstructures |
WO2014067933A1 (en) | 2012-10-31 | 2014-05-08 | C-Lecta Gmbh | Bioactive carrier preparation for enhanced safety in care products and food |
KR101494881B1 (ko) | 2013-05-06 | 2015-02-24 | 한국과학기술연구원 | 설퍼 옥시도리덕타아제 결합 형광 화합물을 이용한 바실러스 서브틸리스 검출 방법 |
CN105866316B (zh) * | 2016-06-24 | 2018-03-30 | 曲阜师范大学 | 一种同时检测食品中氨基酸和生物胺的分析方法 |
CN110954517A (zh) * | 2019-12-11 | 2020-04-03 | 西北大学 | 还原性糖的一步荧光衍生方法及其应用 |
CN113514445B (zh) * | 2021-08-05 | 2023-12-26 | 荆州市第一人民医院(荆州市肿瘤医院、长江大学附属第一医院) | 一种基于荧光偏振的去泛素化酶活性检测方法 |
CN114166807B (zh) * | 2021-11-22 | 2023-12-12 | 中国农业大学 | 用于检测多粘菌素的荧光偏振免疫分析方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3686071T2 (de) * | 1985-02-25 | 1993-01-07 | Abbott Lab | Polarisierter fluoreszenztest von makromolekularen hydrolasen. |
MX9206979A (es) * | 1991-12-04 | 1993-07-01 | Novo Nordisk As | Metodo de clonacion de proteinas en levaduras |
JPH0723800A (ja) * | 1993-07-08 | 1995-01-27 | Tanabe Seiyaku Co Ltd | 核酸の検出方法 |
GB9519393D0 (en) * | 1995-09-22 | 1995-11-22 | Univ Edinburgh | Novel compounds and assay method |
-
1999
- 1999-03-05 AU AU26122/99A patent/AU2612299A/en not_active Abandoned
- 1999-03-05 EP EP99906092A patent/EP1058738B1/en not_active Expired - Lifetime
- 1999-03-05 PT PT99906092T patent/PT1058738E/pt unknown
- 1999-03-05 CA CA002322488A patent/CA2322488A1/en not_active Abandoned
- 1999-03-05 WO PCT/DK1999/000112 patent/WO1999045143A2/en active IP Right Grant
- 1999-03-05 DK DK99906092T patent/DK1058738T3/da active
- 1999-03-05 CN CNA2004100586739A patent/CN1560609A/zh active Pending
- 1999-03-05 AT AT99906092T patent/ATE208828T1/de active
- 1999-03-05 JP JP2000534674A patent/JP2002505114A/ja active Pending
- 1999-03-05 CN CNB998051519A patent/CN1167810C/zh not_active Expired - Fee Related
- 1999-03-05 ES ES99906092T patent/ES2168848T3/es not_active Expired - Lifetime
- 1999-03-05 DE DE69900465T patent/DE69900465T2/de not_active Expired - Lifetime
- 1999-03-05 EP EP01118580A patent/EP1156122A3/en not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
ATE208828T1 (de) | 2001-11-15 |
DE69900465D1 (de) | 2001-12-20 |
JP2002505114A (ja) | 2002-02-19 |
DE69900465T2 (de) | 2002-09-05 |
CN1297487A (zh) | 2001-05-30 |
EP1156122A2 (en) | 2001-11-21 |
AU2612299A (en) | 1999-09-20 |
DK1058738T3 (da) | 2002-03-11 |
WO1999045143A3 (en) | 1999-10-21 |
EP1156122A3 (en) | 2005-02-09 |
CN1167810C (zh) | 2004-09-22 |
CN1560609A (zh) | 2005-01-05 |
EP1058738B1 (en) | 2001-11-14 |
WO1999045143A2 (en) | 1999-09-10 |
PT1058738E (pt) | 2002-05-31 |
EP1058738A2 (en) | 2000-12-13 |
CA2322488A1 (en) | 1999-09-10 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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