ATE321877T1 - Fluoreszenz-verfahren zum nachweis von proteasen oder peptidasen - Google Patents

Fluoreszenz-verfahren zum nachweis von proteasen oder peptidasen

Info

Publication number
ATE321877T1
ATE321877T1 AT01921512T AT01921512T ATE321877T1 AT E321877 T1 ATE321877 T1 AT E321877T1 AT 01921512 T AT01921512 T AT 01921512T AT 01921512 T AT01921512 T AT 01921512T AT E321877 T1 ATE321877 T1 AT E321877T1
Authority
AT
Austria
Prior art keywords
peptidases
fluorescence method
detecting
proteases
pya
Prior art date
Application number
AT01921512T
Other languages
English (en)
Inventor
Bernard Pierre Roques
Nathalie Luciani
Marie-Claude Fournie-Zaluski
Rocquigny Hugues De
Original Assignee
Inst Nat Sante Rech Med
Centre Nat Rech Scient
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Nat Sante Rech Med, Centre Nat Rech Scient filed Critical Inst Nat Sante Rech Med
Application granted granted Critical
Publication of ATE321877T1 publication Critical patent/ATE321877T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/5754—Endothelin, vasoactive intestinal contractor [VIC]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96486—Metalloendopeptidases (3.4.24)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
AT01921512T 2000-04-07 2001-04-06 Fluoreszenz-verfahren zum nachweis von proteasen oder peptidasen ATE321877T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0004507A FR2807523B1 (fr) 2000-04-07 2000-04-07 UTILISATION D'UN RESTE PyA-(Z)x-pNF A DES FINS DE DETECTION, IDENTIFICATION ET/OU DOSAGE PAR FLUORESCENCE

Publications (1)

Publication Number Publication Date
ATE321877T1 true ATE321877T1 (de) 2006-04-15

Family

ID=8849025

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01921512T ATE321877T1 (de) 2000-04-07 2001-04-06 Fluoreszenz-verfahren zum nachweis von proteasen oder peptidasen

Country Status (9)

Country Link
US (1) US7169574B2 (de)
EP (1) EP1268850B1 (de)
JP (1) JP4966469B2 (de)
AT (1) ATE321877T1 (de)
CA (1) CA2405183C (de)
DE (1) DE60118350T2 (de)
ES (1) ES2256224T3 (de)
FR (1) FR2807523B1 (de)
WO (1) WO2001077369A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2903495A1 (fr) * 2006-07-06 2008-01-11 Pharmaleads Soc Par Actions Si PROCEDE DE DETECTION D'INTERACTIONS A L'AIDE DU RAPPORTEUR DE FLUORESCENCE Pya
FR2931151A1 (fr) * 2008-05-13 2009-11-20 Pharmaleads Soc Par Actions Si Nouveaux derives d'amino-acides, leur procede de preparation et leur utilisation therapeutique
FR2963022B1 (fr) * 2010-07-21 2012-08-31 Pharmaleads Nouveaux substrats a fluorescence reprimee, leur preparation et leur utilisation pour l'identification, la detection et le dosage de legionella pneumophila
CN115047092B (zh) * 2022-04-07 2023-10-20 济宁医学院 一种血管紧张素转移酶ii抑制剂的筛选方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4966848A (en) * 1988-02-08 1990-10-30 The General Hospital Corporation Isolation, purification, characterization, cloning and sequencing of N α-acetyltransferase
US5223421A (en) * 1989-10-25 1993-06-29 The General Hospital Corporation Identification of methionine Nα-acetyltransferase
DE4313232A1 (de) * 1993-04-22 1994-10-27 Basf Ag Methode zur Bestimmung der Aktivität des Endothelin-Konversionsenzyms (ECE)
US5506115A (en) * 1994-04-29 1996-04-09 G. D. Searle & Co. Reagent and method for determining activity of herpes protease
US5688489A (en) * 1995-09-15 1997-11-18 Resolution Pharmaceuticals, Inc. Non-receptor mediated imaging agents
FR2745576B1 (fr) * 1996-03-04 1998-06-19 Inst Nat Sante Rech Med Nouveaux peptides radioactifs, leur preparation, et leur utilisation pour le test de selection d'inhibiteurs de l'enzyme de conversion de l'endotheline

Also Published As

Publication number Publication date
US20040214256A1 (en) 2004-10-28
CA2405183C (fr) 2012-06-05
FR2807523A1 (fr) 2001-10-12
US7169574B2 (en) 2007-01-30
EP1268850A1 (de) 2003-01-02
JP4966469B2 (ja) 2012-07-04
CA2405183A1 (fr) 2001-10-18
FR2807523B1 (fr) 2002-08-30
ES2256224T3 (es) 2006-07-16
EP1268850B1 (de) 2006-03-29
DE60118350T2 (de) 2006-10-19
DE60118350D1 (de) 2006-05-18
WO2001077369A1 (fr) 2001-10-18
JP2003530370A (ja) 2003-10-14

Similar Documents

Publication Publication Date Title
ATE431427T1 (de) Verbessertes verfahren zum nachweis von atp
ATE272059T1 (de) Organische salze von isoleucyl-thiazolidin und - pyrrolidin und ihre verwendung als dipeptidylpeptidase-inhibitoren
ATE409238T1 (de) Nachweis der hybridisierung von nukleinsäuren durch fluoreszenzpolarisation
DE122012000015I1 (de) Peptidomimetika als protease inhibitoren.
ATE439452T1 (de) Verfahren zum nachweis von analyten mit hilfe von halbleiternanokrystallen
DE60215339D1 (de) Spezifische detektionsmedien für staphylococcus aureus und identifizierungs- und/oder zaehlmethode unter verwendung besagter detektionsmedien
ATE377654T1 (de) Ubiquitin-ligase-assay
DE60222667D1 (de) Fluorpyrrolidine als dipeptidylpeptidaseinhibitoren
DE69905582D1 (de) Heterocyclische verbindungen als rotamase-enzymen inhibitoren
TR200003071T2 (tr) Sistein-proteazı önleyici etkiye sahip yeni heterosiklik ikame edilmiş amidler
AU7532501A (en) Method for increasing luminescence assay sensitivity
EA200300880A1 (ru) Способ детекции нуклеотидного полиморфизма
TW200700725A (en) Detection of nucleic acid associated with disease
EP1065213A3 (de) HCV Polymerase die für eine Krystalstruktur Analyse geeignet ist, und Verfahren zur Benutzung diesem Enzym
WO2005121354A3 (fr) Substrats peptidiques reconnus par la toxine botulique de type a, bont/a et leurs utilisations
DK1058738T3 (da) Fremgangsmåde til screening, baseret på fluorescens-polarisering
ATE347105T1 (de) Verfahren, assay und kit zur quantifizierung von hiv-proteasehemmer
ATE333510T1 (de) Enzymatische messung von mycophenolsäure
SG159382A1 (en) Effectors of innate immunity
ATE321877T1 (de) Fluoreszenz-verfahren zum nachweis von proteasen oder peptidasen
ATE540966T1 (de) Von phenoxazinon abgeleitete neue enzymsubstrate und deren verwendung als entwickler bei der detektion von mikroorganismen mit peptidaseaktivität
DE69923676D1 (de) Bestimmte heteroaryl substituierte thiol inhibitoren von endothelin wechselnden enzymen
ATE307142T1 (de) Zweifarbiges fluorimetrisches proteaseassay
DE69623328T2 (de) Verwendung von mannanasen als schleimkontrollmittel
ATE487797T1 (de) Verfahren zum nachweis von transferase- enzymaktivität

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties