DK0894091T3 - Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsning - Google Patents
Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsningInfo
- Publication number
- DK0894091T3 DK0894091T3 DK97914167T DK97914167T DK0894091T3 DK 0894091 T3 DK0894091 T3 DK 0894091T3 DK 97914167 T DK97914167 T DK 97914167T DK 97914167 T DK97914167 T DK 97914167T DK 0894091 T3 DK0894091 T3 DK 0894091T3
- Authority
- DK
- Denmark
- Prior art keywords
- purifying
- protein
- proteinaceous solution
- cellulase
- crystalline form
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 108010059892 Cellulase Proteins 0.000 abstract 2
- 229940106157 cellulase Drugs 0.000 abstract 2
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 abstract 1
- 238000002425 crystallisation Methods 0.000 abstract 1
- 230000008025 crystallization Effects 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 150000002576 ketones Chemical class 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
- C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
- Y10S435/816—Enzyme separation or purification by solubility
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK30596 | 1996-03-15 | ||
| PCT/DK1997/000113 WO1997034919A1 (en) | 1996-03-15 | 1997-03-14 | Method for purification of a protein from a protein containing solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0894091T3 true DK0894091T3 (da) | 2003-06-10 |
Family
ID=8092063
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK97914167T DK0894091T3 (da) | 1996-03-15 | 1997-03-14 | Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsning |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6087148A (cs) |
| EP (1) | EP0894091B1 (cs) |
| JP (1) | JP2000506534A (cs) |
| CN (1) | CN1216899C (cs) |
| AT (1) | ATE233784T1 (cs) |
| AU (1) | AU2151997A (cs) |
| DE (1) | DE69719497T2 (cs) |
| DK (1) | DK0894091T3 (cs) |
| IN (1) | IN190120B (cs) |
| WO (1) | WO1997034919A1 (cs) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0894091T3 (da) * | 1996-03-15 | 2003-06-10 | Novozymes As | Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsning |
| CA2330476A1 (en) * | 1998-04-27 | 1999-11-04 | Altus Biologics Inc. | Stabilized protein crystals, formulations containing them and methods of making them |
| DE19859876A1 (de) * | 1998-12-23 | 2000-06-29 | Fraunhofer Ges Forschung | Verfahren zur Kristallisation aus Lösungen |
| GB9910975D0 (en) | 1999-05-13 | 1999-07-14 | Univ Strathclyde | Rapid dehydration of proteins |
| US6663602B2 (en) | 2000-06-16 | 2003-12-16 | Novo Nordisk A/S | Injection device |
| DE60231534D1 (de) * | 2001-10-25 | 2009-04-23 | Kyowa Hakko Kogyo Kk | Kristall von oxidiertem glutathion und verfahren zu dessen herstellung |
| DE60326012D1 (de) * | 2002-11-01 | 2009-03-12 | Bayer Healthcare Llc | Verfahren zur Konzentration von Proteinen |
| US7686786B2 (en) | 2004-10-21 | 2010-03-30 | Novo Nordiks A/S | Dial-down mechanism for wind-up pen |
| US8088900B2 (en) | 2006-03-30 | 2012-01-03 | Novo Nordisk A/S | Two-phase precipitation of proteins |
| DE602007004972D1 (de) | 2006-05-16 | 2010-04-08 | Novo Nordisk As | Getriebemechanismus für ein injektionsgerät |
| JP5253387B2 (ja) | 2006-05-18 | 2013-07-31 | ノボ・ノルデイスク・エー／エス | モード固定手段を備えている注入装置 |
| DE102008029401A1 (de) * | 2008-06-23 | 2009-12-24 | Bayer Technology Services Gmbh | Verfahren zur Kristallisation von Peptiden und Proteinen |
| FR2943686B1 (fr) * | 2009-03-30 | 2013-11-01 | Roquette Freres | Procede d'obtention d'une preparation de beta-amylases a partir des fractions solubles de plantes amidonnieres |
| JP6069351B2 (ja) | 2011-12-29 | 2017-02-01 | ノボ・ノルデイスク・エー／エス | ダイアルアップ／ダイアルダウン投与機構を有するねじりバネ式ねじ巻き自動注射器 |
| WO2023200284A1 (ko) * | 2022-04-15 | 2023-10-19 | 재단법인대구경북과학기술원 | 생체 시료 내 지질 연관 단백질의 질량 분석을 위한 지질 연관 단백질의 정제 및 농축 방법 |
| CN119677762A (zh) * | 2022-08-10 | 2025-03-21 | 阿拉食品公司 | 使用多种蛋白质进料使能够在盐溶条件下结晶的蛋白质结晶 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3664926A (en) * | 1970-02-26 | 1972-05-23 | Merck & Co Inc | Crystallization of l-asparaginase |
| DE4001238A1 (de) * | 1990-01-18 | 1991-07-25 | Basf Ag | Verfahren zur anreicherung bzw. reinigung von biomolekuelen |
| DK115890D0 (da) * | 1990-05-09 | 1990-05-09 | Novo Nordisk As | Enzym |
| DK83093D0 (da) * | 1993-07-09 | 1993-07-09 | Novo Nordisk As | Fremgangsmaade |
| DK0894091T3 (da) * | 1996-03-15 | 2003-06-10 | Novozymes As | Fremgangsmåde til oprensning af et protein fra en proteinholdig opløsning |
-
1997
- 1997-03-14 DK DK97914167T patent/DK0894091T3/da active
- 1997-03-14 AU AU21519/97A patent/AU2151997A/en not_active Abandoned
- 1997-03-14 CN CN971930430A patent/CN1216899C/zh not_active Expired - Lifetime
- 1997-03-14 EP EP97914167A patent/EP0894091B1/en not_active Expired - Lifetime
- 1997-03-14 WO PCT/DK1997/000113 patent/WO1997034919A1/en not_active Ceased
- 1997-03-14 JP JP9533055A patent/JP2000506534A/ja active Pending
- 1997-03-14 DE DE69719497T patent/DE69719497T2/de not_active Expired - Lifetime
- 1997-03-14 AT AT97914167T patent/ATE233784T1/de not_active IP Right Cessation
- 1997-03-17 IN IN552MA1997 patent/IN190120B/en unknown
-
1998
- 1998-08-28 US US09/143,206 patent/US6087148A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CN1213377A (zh) | 1999-04-07 |
| EP0894091B1 (en) | 2003-03-05 |
| JP2000506534A (ja) | 2000-05-30 |
| DE69719497D1 (de) | 2003-04-10 |
| EP0894091A1 (en) | 1999-02-03 |
| DE69719497T2 (de) | 2003-10-30 |
| ATE233784T1 (de) | 2003-03-15 |
| US6087148A (en) | 2000-07-11 |
| IN190120B (cs) | 2003-06-14 |
| AU2151997A (en) | 1997-10-10 |
| CN1216899C (zh) | 2005-08-31 |
| WO1997034919A1 (en) | 1997-09-25 |
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