DK0453047T3 - Penicillin G acylase, et gen kodende herfor og en fremgangsmåde til fremstilling af dette enzym - Google Patents

Penicillin G acylase, et gen kodende herfor og en fremgangsmåde til fremstilling af dette enzym

Info

Publication number
DK0453047T3
DK0453047T3 DK91200929.7T DK91200929T DK0453047T3 DK 0453047 T3 DK0453047 T3 DK 0453047T3 DK 91200929 T DK91200929 T DK 91200929T DK 0453047 T3 DK0453047 T3 DK 0453047T3
Authority
DK
Denmark
Prior art keywords
enzyme
acylase
penicillin
producing
gene
Prior art date
Application number
DK91200929.7T
Other languages
Danish (da)
English (en)
Inventor
Wilhelmus Johannes Quax
Original Assignee
Gist Brocades Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Application granted granted Critical
Publication of DK0453047T3 publication Critical patent/DK0453047T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01011—Penicillin amidase (3.5.1.11), i.e. penicillin-amidohydrolase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/829—Alcaligenes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK91200929.7T 1990-04-18 1991-04-18 Penicillin G acylase, et gen kodende herfor og en fremgangsmåde til fremstilling af dette enzym DK0453047T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP90200962 1990-04-18
EP90203463 1990-12-20

Publications (1)

Publication Number Publication Date
DK0453047T3 true DK0453047T3 (da) 1995-09-11

Family

ID=26125836

Family Applications (1)

Application Number Title Priority Date Filing Date
DK91200929.7T DK0453047T3 (da) 1990-04-18 1991-04-18 Penicillin G acylase, et gen kodende herfor og en fremgangsmåde til fremstilling af dette enzym

Country Status (10)

Country Link
US (2) US5168048A (pt)
EP (1) EP0453047B1 (pt)
JP (1) JPH04228073A (pt)
KR (1) KR0152667B1 (pt)
AT (1) ATE124724T1 (pt)
DE (1) DE69110952T2 (pt)
DK (1) DK0453047T3 (pt)
ES (1) ES2077151T3 (pt)
IE (1) IE68078B1 (pt)
PT (1) PT97397B (pt)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT97409B (pt) * 1990-04-18 1998-08-31 Gist Brocades Nv Processo para a preparacao de mutantes beta-lactama-acilases por expressao genetica
EP0638649B1 (en) * 1993-08-05 1999-11-17 Dsm N.V. New use of alcaligenes faecalis penicillin G acylase
ATE189477T1 (de) * 1994-08-12 2000-02-15 Dsm Nv Mutierte penicillin-g-acylase gene
US5516679A (en) * 1994-12-23 1996-05-14 Bristol-Myers Squibb Company Penicillin V amidohydrolase gene from Fusarium oxysporum
WO1997020053A2 (en) * 1995-11-27 1997-06-05 Gist-Brocades B.V. Improved process for the production of semi-synthetic cephalosporins via expandase activity on penicillin g
IL129055A (en) 1996-11-05 2005-06-19 Bristol Myers Squibb Co Mutant penicillin g acylases
US6437113B1 (en) * 1998-03-05 2002-08-20 Tularik Inc. Suppressors of death domains
WO2000066751A1 (en) * 1999-04-29 2000-11-09 Dsm N.V. Expression cassette for efficient production of a protein
US20030044884A1 (en) * 2000-10-11 2003-03-06 Usher John J. Synthesis of beta-lactam antibacterials using soluble side chain esters and enzyme acylase
US6699699B2 (en) 2002-03-26 2004-03-02 Synmax Biochemical Co., Ltd. Mutated penicillin expandases
WO2003104446A1 (ja) 2002-06-06 2003-12-18 鐘淵化学工業株式会社 新規アシラーゼ遺伝子
KR100530299B1 (ko) * 2003-08-11 2005-11-22 산도즈 게엠베하 변이 세팔로스포린 ｃ 아실라제 및 이를 이용한 7-ａｃａ 제조방법
ITMI20040016A1 (it) * 2004-01-12 2004-04-12 Antibiotics S P A Cefalosporina c acilasi
CZ300467B6 (cs) * 2007-01-31 2009-05-27 Mikrobiologický ústav AV CR, v.v.i. Sekvence nukleotidu o velikosti 2646 bp, kódující penicilinacylázu, nové konstrukty rekombinantní nukleové kyseliny a rekombinantní mikroorganismy nesoucí tuto sekvenci
TW201022445A (en) * 2008-10-09 2010-06-16 Dsm Ip Assets Bv Method for preparing ε-caprolactam from N-acyl-6-aminocaproic acid
CA2894595A1 (en) 2012-12-14 2014-06-19 Saban Ventures Pty Limited Synergistic disinfection enhancement
US10206404B2 (en) 2012-12-14 2019-02-19 Saban Ventures Pty Limited Disinfectant
CN113913416B (zh) * 2021-10-14 2022-03-22 巢湖学院 一种粪产碱杆菌青霉素g酰化酶的制备工艺
CN115418331B (zh) * 2022-08-29 2024-09-10 新发药业有限公司 一种产青霉素g酰基转移酶自诱导培养基及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3239427A (en) * 1959-10-12 1966-03-08 Pfizer & Co C Production of 6-aminopenicillanic acid
US3272715A (en) * 1962-07-17 1966-09-13 American Home Prod Method for producing and recovering 6-aminopenicillanic acid
BE790075A (fr) * 1971-10-14 1973-04-13 Bayer Ag Procede de preparation de penicillinacylase cristalline pure etproduit obtenu
DE2732301C3 (de) * 1977-07-16 1980-12-11 Roehm Gmbh, 6100 Darmstadt Verfahren zur Herstellung von stabilisierten trägergebundenen Proteinen
DE3330003A1 (de) * 1982-10-21 1984-10-31 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Dna-sequenz und dna-strukturen und sie verwendendes verfahren zur herstellung von penicillin-acylase
JPS6066981A (ja) * 1983-09-12 1985-04-17 アメリカン・ホ−ム・プロダクツ・コ−ポレイシヨン 不溶化ペニシリンアミダ−ゼおよびその製法
ATE105860T1 (de) * 1988-04-08 1994-06-15 Biochemie Gmbh Gen und genprodukt zur herstellung von beta- lactamverbindungen.
DE3909018A1 (de) * 1989-03-18 1990-09-20 Roehm Gmbh Protein-reinigungsverfahren

Also Published As

Publication number Publication date
US5168048A (en) 1992-12-01
KR910018550A (ko) 1991-11-30
DE69110952T2 (de) 1995-11-16
IE911294A1 (en) 1991-10-23
PT97397B (pt) 1998-08-31
JPH04228073A (ja) 1992-08-18
ES2077151T3 (es) 1995-11-16
ATE124724T1 (de) 1995-07-15
US5695978A (en) 1997-12-09
EP0453047A1 (en) 1991-10-23
KR0152667B1 (ko) 1998-10-01
PT97397A (pt) 1992-01-31
DE69110952D1 (de) 1995-08-10
IE68078B1 (en) 1996-05-15
EP0453047B1 (en) 1995-07-05

Similar Documents

Publication Publication Date Title
DK0453047T3 (da) Penicillin G acylase, et gen kodende herfor og en fremgangsmåde til fremstilling af dette enzym
HUP9701652A2 (hu) Eljárás L-aminosav előállítására fermentáció útján
HUP0203704A2 (hu) L-Pankolakton hidroláz és eljárás D-pantolakton előállítására
DK1218530T3 (da) Hidtil ukendte enzymer, der dehydrerer glycerol
ATE417094T1 (de) Verfahren zur herstellung von l-aminosäure
ATE123533T1 (de) Enzymatisches verfahren zur herstellung von 7- aminocephalosporansäure.
DE68915692D1 (de) Fermentationsverfahren zur Herstellung von 2-Keto-L-Gulonsäure.
ATE112574T1 (de) Igg-bindendes protein h, kodierendes gen dafür und verfahren zu seiner herstellung.
ATE171728T1 (de) Trypsintyp-enzyme, dafür kodierende nukleinsäuresequenz und verfahren zur herstellung des enzymes
ES8602116A1 (es) Un procedimiento para hidrolizar almidon con una enzima de alfa-amilasa
ATE461997T1 (de) Neuartige glukose-6-phosphat dehydrogenase
ATE123304T1 (de) Durch rekombinante dns-technologie konstruierte amylolytische enzymproduzierende mikroorganismen und ihre verwendung in gärungsverfahren.
DE60142203D1 (de) Polyhydroxyalkanoat-Synthase und dieses Enzym kodierendes Gen
DK0731843T3 (da) Tyrosinaseaktivatorprotein-fusionsenzym
ATE103329T1 (de) Verfahren zur herstellung von hgiairestriktionsendonuklease und -methylase.
ATE265527T1 (de) Enzym mit pektin-esterase-aktivität
ATE274063T1 (de) Dns, die für ein rekombinantes enzym zur umwandlung von maltose in trehalose kodiert
ATE196927T1 (de) Verfahren zur herstellung von proteaseinhibitoren
ATE105860T1 (de) Gen und genprodukt zur herstellung von beta- lactamverbindungen.
ATE101647T1 (de) Verfahren zur herstellung von aseirestriktionsendonuklease und -methylase.
DE69731451D1 (de) Biotin-Biosynthese-Gene II
ES2108651B1 (es) Procedimiento para incrementar la produccion de penicilina g (bencilpenicilina) en penicillium chrysogenum mediante la expresion del gen pcl.
ATE155171T1 (de) Verfahren zur herstellung von ma
DE69231145D1 (de) Für eine Esterase kodierendes Gen und dieses Gen enthaltender Mikroorganismus
DK1282715T3 (da) Fremgangsmåde til mikrobiel fremstilling af difructoseanhydrid-III, mikroorganisme anvendelig dertil samt enzym med inulase-II-aktivetet og DNA-sekvenser kodende derfor