DE69943292D1 - Verfahren zur Amplifikation und Sequenzierung von Nucleinsäuren und zur Herstellung von Nucleinsäuren mit verminderter thermodynamischer Stabilität - Google Patents

Verfahren zur Amplifikation und Sequenzierung von Nucleinsäuren und zur Herstellung von Nucleinsäuren mit verminderter thermodynamischer Stabilität

Info

Publication number
DE69943292D1
DE69943292D1 DE69943292T DE69943292T DE69943292D1 DE 69943292 D1 DE69943292 D1 DE 69943292D1 DE 69943292 T DE69943292 T DE 69943292T DE 69943292 T DE69943292 T DE 69943292T DE 69943292 D1 DE69943292 D1 DE 69943292D1
Authority
DE
Germany
Prior art keywords
nucleic acids
sequencing
amplification
production
thermodynamic stability
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69943292T
Other languages
German (de)
English (en)
Inventor
Elazar Rabbani
Jannis G Stavrianopolous
James J Donegan
Jack Coleman
Marleen Farmingdale Walner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Enzo Diagnostics Inc
Original Assignee
Enzo Diagnostics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22298492&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69943292(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Enzo Diagnostics Inc filed Critical Enzo Diagnostics Inc
Application granted granted Critical
Publication of DE69943292D1 publication Critical patent/DE69943292D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
DE69943292T 1998-06-24 1999-06-24 Verfahren zur Amplifikation und Sequenzierung von Nucleinsäuren und zur Herstellung von Nucleinsäuren mit verminderter thermodynamischer Stabilität Expired - Lifetime DE69943292D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/104,067 US6743605B1 (en) 1998-06-24 1998-06-24 Linear amplification of specific nucleic acid sequences

Publications (1)

Publication Number Publication Date
DE69943292D1 true DE69943292D1 (de) 2011-05-05

Family

ID=22298492

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69943292T Expired - Lifetime DE69943292D1 (de) 1998-06-24 1999-06-24 Verfahren zur Amplifikation und Sequenzierung von Nucleinsäuren und zur Herstellung von Nucleinsäuren mit verminderter thermodynamischer Stabilität

Country Status (6)

Country Link
US (11) US6743605B1 (https=)
EP (1) EP0971039B1 (https=)
JP (3) JP2000037194A (https=)
CA (1) CA2273064C (https=)
DE (1) DE69943292D1 (https=)
IL (1) IL130477A (https=)

Families Citing this family (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
US8445664B2 (en) 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
ATE374833T1 (de) 1998-11-09 2007-10-15 Eiken Chemical Verfahren zur synthese von nukleinsäuren
JP3974441B2 (ja) * 1998-11-09 2007-09-12 栄研化学株式会社 核酸の合成方法
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
HK1052203A1 (zh) * 1999-11-08 2003-09-05 荣研化学株式会社 检测突变和/或多态性的方法
EP1231281B1 (en) * 1999-11-08 2006-02-01 Eiken Kagaku Kabushiki Kaisha Method of detecting variation or polymorphism
ES2546450T3 (es) * 2000-04-07 2015-09-23 Eiken Kagaku Kabushiki Kaisha Método para amplificar y detectar un ácido nucleico usando ácido nucleico bicatenario como molde
JP2001309785A (ja) * 2000-05-01 2001-11-06 Eiken Chem Co Ltd 遺伝子配列間の相違検出方法
EP1356094B1 (en) 2000-06-26 2010-01-13 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
ATE437225T1 (de) * 2000-09-19 2009-08-15 Eiken Chemical Verfahren zur polynukleotidsynthese
WO2002048402A2 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
WO2002072773A2 (en) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
JP4542312B2 (ja) 2001-03-09 2010-09-15 ニューゲン テクノロジーズ， インコーポレイテッド Ｒｎａ配列の増幅のための方法および組成物
US9777312B2 (en) 2001-06-30 2017-10-03 Enzo Life Sciences, Inc. Dual polarity analysis of nucleic acids
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
JP2005052002A (ja) * 2001-07-06 2005-03-03 Wakunaga Pharmaceut Co Ltd 非環状核酸断片の増幅方法
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
AU2002366098A1 (en) 2001-11-19 2003-06-10 Parallele Bioscience Inc Multiplex pcr
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US6815167B2 (en) 2002-04-25 2004-11-09 Geneohm Sciences Amplification of DNA to produce single-stranded product of defined sequence and length
EP1513958B1 (en) 2002-06-14 2011-08-10 Gen-Probe Incorporated Compositions for detecting hepatitis b virus
GB0218215D0 (en) * 2002-08-06 2002-09-11 Tepnel Medical Ltd Amplification of nucleic acids
WO2004040019A1 (ja) * 2002-10-29 2004-05-13 Riken 核酸の増幅法
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
US8206902B2 (en) 2003-12-25 2012-06-26 Riken Method of amplifying nucleic acid and method of detecting mutated nucleic acid using the same
US7575863B2 (en) * 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
CA2476030A1 (en) * 2004-06-09 2005-12-09 Wilsun Xu A power signaling based technique for detecting islanding conditions in electric power distribution systems
US8124334B2 (en) * 2004-07-06 2012-02-28 Enzo Biochem, Inc. Selective detection of oncogenic HPV
WO2006034387A1 (en) * 2004-09-21 2006-03-30 Applera Corporation TWO-COLOR REAL-TIME/END-POINT QUANTITATION OF MICRORNAS (miRNAs)
EP1696040A1 (en) * 2005-02-28 2006-08-30 Eurofins Viralliance, Inc. Method of genotyping and phenotyping hepatitis B viruses resistant to antiviral molecules
EP1871897B1 (en) 2005-04-12 2010-09-29 Olink AB Methods for production of oligonucleotides
EP1871896B1 (en) * 2005-04-12 2015-06-03 Olink AB Circle probes and their use in the identification of biomolecules
KR101931899B1 (ko) 2005-05-09 2018-12-21 테라노스, 인코포레이티드 현장진료 유체 시스템 및 그 용도
AU2006287548A1 (en) * 2005-09-06 2007-03-15 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
WO2007030759A2 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
US9757455B2 (en) * 2005-11-28 2017-09-12 Johnson & Johnson Consumer Inc. Oral therapeutic compound delivery system
JP4580875B2 (ja) 2006-01-20 2010-11-17 株式会社東芝 核酸検出法のためのプライマーの設計方法
US11287421B2 (en) 2006-03-24 2022-03-29 Labrador Diagnostics Llc Systems and methods of sample processing and fluid control in a fluidic system
US8007999B2 (en) 2006-05-10 2011-08-30 Theranos, Inc. Real-time detection of influenza virus
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US7501254B2 (en) 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
US8012744B2 (en) 2006-10-13 2011-09-06 Theranos, Inc. Reducing optical interference in a fluidic device
US20080113391A1 (en) 2006-11-14 2008-05-15 Ian Gibbons Detection and quantification of analytes in bodily fluids
EP2108048A1 (en) * 2007-01-17 2009-10-14 Meridian Bioscience, Inc. Stable reagents and kits useful in loop-mediated isothermal amplification (lamp)
US8158430B1 (en) 2007-08-06 2012-04-17 Theranos, Inc. Systems and methods of fluidic sample processing
ES2447875T3 (es) 2007-10-02 2014-03-13 Theranos, Inc. Dispositivos modulares para punto de cuidados y usos de los mismos
US8420323B2 (en) 2007-11-14 2013-04-16 Fujifilm Corporation Nucleic acid amplification method
US20090162845A1 (en) 2007-12-20 2009-06-25 Elazar Rabbani Affinity tag nucleic acid and protein compositions, and processes for using same
US20090203531A1 (en) * 2008-02-12 2009-08-13 Nurith Kurn Method for Archiving and Clonal Expansion
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
JP5843614B2 (ja) * 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
WO2010114842A1 (en) 2009-03-30 2010-10-07 Ibis Biosciences, Inc. Bioagent detection systems, devices, and methods
EP2272980A1 (en) * 2009-07-09 2011-01-12 Bioesplora SRL Molecular probes for target nucleic acid detection and quantification
WO2011014811A1 (en) 2009-07-31 2011-02-03 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
CA3081708C (en) 2009-10-19 2023-10-03 Theranos Ip Company, Llc Integrated health data capture and analysis system
EP2504450B1 (en) * 2009-11-23 2016-07-06 Swift Biosciences, Inc. Devices to extend single stranded target molecules
EP4024029A3 (en) 2011-01-21 2022-09-14 Labrador Diagnostics LLC Systems and methods for sample use maximization
AU2012288629B2 (en) 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US9109226B2 (en) * 2011-09-01 2015-08-18 New England Biolabs, Inc. Synthetic nucleic acids for polymerization reactions
US9416153B2 (en) 2011-10-11 2016-08-16 Enzo Life Sciences, Inc. Fluorescent dyes
WO2013101935A1 (en) 2011-12-27 2013-07-04 Ibis Biosciences, Inc. Bioagent detection oligonucleotides
WO2013101783A2 (en) 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
US20130184165A1 (en) 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
JP6271505B2 (ja) 2012-04-10 2018-01-31 オックスフォード ナノポール テクノロジーズ リミテッド 変異体ライセニンポア
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
EP3511424B1 (en) 2012-08-03 2023-10-18 University Of Washington Through Its Center For Commercialization Compositions and methods for improving nanopore sequencing
US9617607B2 (en) 2013-01-08 2017-04-11 Enzo Biochem, Inc. Diagnosis and treatment of viral diseases
WO2014110127A1 (en) 2013-01-08 2014-07-17 Enzo Biochem, Inc. Diagnosis and treatment of viral diseases
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
KR102168813B1 (ko) 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
AU2014233145A1 (en) 2013-03-15 2015-09-17 Theranos Ip Company, Llc Nucleic acid amplification
JP2016521120A (ja) * 2013-03-15 2016-07-21 セラノス， インコーポレイテッド 核酸増幅
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
AU2014233152A1 (en) 2013-03-15 2015-09-17 Theranos Ip Company, Llc Nucleic acid amplification
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
WO2015035260A1 (en) 2013-09-06 2015-03-12 Theranos, Inc. Systems and methods for detecting infectious diseases
JP6749243B2 (ja) 2014-01-22 2020-09-02 オックスフォード ナノポール テクノロジーズ リミテッド １つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US10093918B2 (en) 2014-06-04 2018-10-09 Lucigen Corporation Sample collection and analysis devices
CN106661600B (zh) 2014-07-16 2021-04-06 唐恩生物科技股份有限公司 用于扩增核酸样品的等温方法
US10400014B2 (en) 2014-09-01 2019-09-03 Oxford Nanopore Technologies Ltd. Mutant CsgG pores
JP2017531424A (ja) * 2014-09-17 2017-10-26 セラノス， インコーポレイテッドＴｈｅｒａｎｏｓ， Ｉｎｃ． ハイブリッド多元ステップ核酸増幅
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
US11559801B2 (en) 2014-11-03 2023-01-24 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
KR102837999B1 (ko) 2015-12-08 2025-07-24 카트호리이케 유니버시타이트 로이펜 변형된 나노세공, 그를 포함하는 조성물, 및 그의 용도
WO2017142825A2 (en) * 2016-02-18 2017-08-24 Merck Sharp & Dohme Corp. N3-substituted iminopyrimidinones as antimalarial agents
AU2017225374B2 (en) 2016-03-02 2020-03-05 Oxford Nanopore Technologies Limited Mutant pore
WO2017174990A1 (en) 2016-04-06 2017-10-12 Oxford Nanopore Technologies Limited Mutant pore
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
PL3469365T3 (pl) 2016-06-09 2023-04-03 The Regents Of The University Of California Zatężanie biomarkera i amplifikacja sygnału do zastosowania w testach immunologicznych na bazie papieru
US10822648B1 (en) 2016-07-29 2020-11-03 Labrador Diagnostics Llc Hybrid multi-step nucleic acid amplification
US10851410B2 (en) 2017-01-04 2020-12-01 Mgi Tech Co., Ltd. Nucleic acid sequencing using affinity reagents
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
EP3642363B1 (en) * 2017-06-20 2025-12-24 Bio-Rad Laboratories, Inc. Mda using bead oligonucleotide
WO2019002893A1 (en) 2017-06-30 2019-01-03 Vib Vzw NEW PROTEIN PORES
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method

Family Cites Families (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE265429C (https=) 1911-12-15 1913-10-10
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5260433A (en) 1982-06-23 1993-11-09 Enzo Diagnostics, Inc. Saccharide specific binding system labeled nucleotides
US5241060A (en) 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US5171534A (en) 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
US4707440A (en) 1984-01-30 1987-11-17 Enzo Biochem, Inc. Nucleic acid hybridization assay and detectable molecules useful in such assay
US5346603A (en) 1984-03-29 1994-09-13 The Board Of Regents Of The University Of Nebraska DNA sequencing
US4729947A (en) 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
US5230781A (en) 1984-03-29 1993-07-27 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes
US5360523A (en) 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US4749647A (en) * 1984-06-22 1988-06-07 Genetic Systems Corporation Polymerization-induced separation assay using recognition pairs
GB8421967D0 (en) * 1984-08-30 1984-10-03 Hickory Springs Mfg Co Polyurethane foams
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5093232A (en) 1985-12-11 1992-03-03 Chiron Corporation Nucleic acid probes
JP3293820B2 (ja) 1985-12-13 2002-06-17 エンゾ− バイオケム インコ−ポレイテツド 標的ポリヌクレオチドにハイブリツド形成するための新規な一工程方法とポリヌクレオチド化合物
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5047519A (en) * 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
US5032570A (en) 1987-08-04 1991-07-16 Hitachi Metals, Ltd. Method for producing ceramic superconducting material using intermediate products
DD265429A1 (de) * 1987-09-30 1989-03-01 Adl Inst Phytopathologie Verfahren zur nichtradioaktiven markierung von polynukleotiden
AU622426B2 (en) 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
US5260428A (en) * 1988-07-11 1993-11-09 Boehringer Mannheim Gmbh Agent for the detection of substances with hydrolase activity
US5508178A (en) 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
US5503975A (en) * 1989-03-01 1996-04-02 City Of Hope Mechanism based inhibitors of DNA methyltransferase
JPH0750041Y2 (ja) 1990-01-17 1995-11-15 大同特殊鋼株式会社 連続鋳造設備におけるタンディッシュ交換装置
WO1992000989A1 (en) 1990-07-10 1992-01-23 Imperial Chemical Industries Plc Non-isotopic nucleic acid labelling method
US5595891A (en) 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
US5439793A (en) 1990-07-19 1995-08-08 Syntex (U.S.A.) Inc. Method for producing a polynucleotide having an intramolecularly base-paired structure
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US5569582A (en) 1991-07-15 1996-10-29 Institute Of Molecular Biology & Technology Rapid amplification and detection of nucleic acids
US5714323A (en) 1991-08-30 1998-02-03 The University Of Medecine And Dentistry Of New Jersey Over expression of single-stranded molecules
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US5612199A (en) 1991-10-11 1997-03-18 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
EP0656065A1 (en) 1991-10-31 1995-06-07 University Of Pittsburgh Reverse dot blot hybridization using tandem head-to-tail monomers containing probes synthesized by staggered complementary primers
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
ZA929351B (en) * 1991-12-11 1993-06-04 Igen Inc Electrochemiluminescent label for DNA assays.
FR2687679B1 (fr) 1992-02-05 1994-10-28 Centre Nat Rech Scient Oligothionucleotides.
US5462854A (en) 1993-04-19 1995-10-31 Beckman Instruments, Inc. Inverse linkage oligonucleotides for chemical and enzymatic processes
US5470723A (en) 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
WO1995002690A1 (en) 1993-07-13 1995-01-26 Abbott Laboratories Nucleotide sequences and process for amplifying and detection of hepatitis b virus
CA2105364A1 (en) * 1993-09-01 1995-03-02 Eric T. Kool Stem-loop oligonucleotides containing parallel and antiparallel binding domains
DE4331670A1 (de) 1993-09-17 1995-03-23 Hoechst Ag Neue Antisense-Oligonucleotide gegen HSV-1 sowie deren Herstellung
US5470184A (en) * 1994-01-14 1995-11-28 Chandler; Charles W. Screw hole plug
US5462584A (en) 1994-03-21 1995-10-31 Gas Research Institute Process for dehydrating natural gas
FR2721945B1 (fr) 1994-07-04 1996-10-18 David Fabrice Accroissement genique, un procede d'amplicication genique isotherme et ses applications
FR2726277B1 (fr) * 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
US5665545A (en) * 1994-11-28 1997-09-09 Akzo Nobel N.V. Terminal repeat amplification method
US5798276A (en) 1995-06-07 1998-08-25 Molecular Probes, Inc. Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability
AT402203B (de) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
WO1997004131A1 (en) * 1995-07-21 1997-02-06 Forsyth Dental Infirmary For Children Single primer amplification of polynucleotide hairpins
US6114150A (en) 1995-11-29 2000-09-05 Yale University Amplification of nucleic acids
GB9609441D0 (en) 1996-05-04 1996-07-10 Zeneca Ltd Process
US5821356A (en) 1996-08-12 1998-10-13 The Perkin Elmer Corporation Propargylethoxyamino nucleotides
JPH10312826A (ja) 1997-03-10 1998-11-24 Sanyo Electric Co Ltd 非水電解質電池及びその充電方法
EP0973788A1 (en) 1997-04-02 2000-01-26 Amersham Pharmacia Biotech UK Limited Tricyclic base analogues
GB9717061D0 (en) 1997-08-13 1997-10-15 Tepnel Medical Ltd Amplification of nucleic acids
US6087099A (en) * 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
GB9810865D0 (en) 1998-05-20 1998-07-22 Zeneca Ltd Nucleic acid sequence identification
US5991238A (en) 1998-06-09 1999-11-23 Western Atlas International, Inc. Weighted backus filter method of combining dual sensor traces
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
ATE374833T1 (de) 1998-11-09 2007-10-15 Eiken Chemical Verfahren zur synthese von nukleinsäuren
US6114123A (en) * 1999-06-14 2000-09-05 Incyte Pharmaceuticals, Inc. Lipocalin family protein
EP1231281B1 (en) 1999-11-08 2006-02-01 Eiken Kagaku Kabushiki Kaisha Method of detecting variation or polymorphism
WO2001034790A1 (en) 1999-11-08 2001-05-17 Eiken Kagaku Kabushiki Kaisha Method for synthesizing nucleic acid

Also Published As

Publication number Publication date
US20030170682A1 (en) 2003-09-11
JP4493330B2 (ja) 2010-06-30
US20050260573A1 (en) 2005-11-24
IL130477A (en) 2004-03-28
JP4675141B2 (ja) 2011-04-20
US6743605B1 (en) 2004-06-01
US7485417B2 (en) 2009-02-03
CA2273064A1 (en) 1999-12-24
US20030165939A1 (en) 2003-09-04
US8288092B2 (en) 2012-10-16
US20030165936A1 (en) 2003-09-04
JP2004141170A (ja) 2004-05-20
US20030104460A1 (en) 2003-06-05
EP0971039A3 (en) 2004-01-28
US20090286285A1 (en) 2009-11-19
IL130477A0 (en) 2000-06-01
US20030170681A1 (en) 2003-09-11
US20030165938A1 (en) 2003-09-04
US8133989B2 (en) 2012-03-13
US7264930B2 (en) 2007-09-04
US20090263789A1 (en) 2009-10-22
US6764821B1 (en) 2004-07-20
US7468245B2 (en) 2008-12-23
EP0971039B1 (en) 2011-03-23
JP2005270108A (ja) 2005-10-06
JP2000037194A (ja) 2000-02-08
EP0971039A2 (en) 2000-01-12
CA2273064C (en) 2008-04-01
US8486633B2 (en) 2013-07-16
US7713691B2 (en) 2010-05-11

Similar Documents

Publication Publication Date Title
DE69943292D1 (de) Verfahren zur Amplifikation und Sequenzierung von Nucleinsäuren und zur Herstellung von Nucleinsäuren mit verminderter thermodynamischer Stabilität
DE69928683D1 (de) Verfahren zur amplifikation und zur sequenzierung von nukleinsäuren
DE59912604D1 (de) Verfahren zur isolierung und aufreinigung von nucleinsäuren
DE60002793D1 (de) Verfahren zur Herstellung von Acrolein und Acrylsäure
DE69727613D1 (de) Verfahren zur Herstellung von porösem Material
DE69908998D1 (de) Verfahren zur Herstellung von Kohlenstoff
ATE220658T1 (de) Verbessertes verfahren zur gleichzeitigen herstellung von 6-aminocapronitril und hexamethylendiamin
DE69532462D1 (de) Verfahren zur Herstellung von aliphatischen Polyester
DE59812758D1 (de) Verfahren zur herstellung von 8-methoxy-chinoloncarbonsäuren
DE69809828D1 (de) Verfahren zur Herstellung von Nudeln
DE69909181D1 (de) Verfahren zur Herstellung von Acrolein und Acrylsäure
DE69940891D1 (de) Verfahren zur herstellung von transglutaminase
DE69727977D1 (de) Verfahren zur simultanen herstellung von acrylnitril und acrylsäure
DE69712157D1 (de) Verfahren zur Herstellung von Acrylnitril
DE69637616D1 (de) Verfahren zur herstellung von nukleinsäuren
DE59712415D1 (de) Verfahren zur amplifikation von nukleinsäure
ATE251112T1 (de) Verbessertes verfahren zur gleichzeitigen herstellung von 6-aminocapronitril und hexamethylendiamin
EP0981535A4 (en) Methods for production and purification of nucleic acid molecules
ATA78096A (de) Biologisches material frei von viralen und molekularen pathogenen und verfahren zur herstellung
DE59606220D1 (de) Verfahren zur Herstellung von Hohlkörpern
ATE291095T1 (de) Enzymatisches verfahren zur stereoselektiven herstellung von therapeutischen amiden
DE69805336D1 (de) Verfahren zur Herstellung von Heizkörpergliedern
DE59905990D1 (de) Verfahren zur Herstellung von Trifluorbenzoesäuren
DE69903398D1 (de) Verfahren zur Herstellung von 2-Alkyl-3-hydroxybenzoesäuren
DE69815476D1 (de) Verfahren zur Herstellung von Trehalose und Zuckeralkoholen