DE69939829D1 - Stärke "debranching-enzyme" - Google Patents

Stärke "debranching-enzyme"

Info

Publication number
DE69939829D1
DE69939829D1 DE69939829T DE69939829T DE69939829D1 DE 69939829 D1 DE69939829 D1 DE 69939829D1 DE 69939829 T DE69939829 T DE 69939829T DE 69939829 T DE69939829 T DE 69939829T DE 69939829 D1 DE69939829 D1 DE 69939829D1
Authority
DE
Germany
Prior art keywords
enzyme
parent
variant
debranching enzyme
strength
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69939829T
Other languages
English (en)
Inventor
Henrik Bisgard-Frantzen
Allan Svendsen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes AS filed Critical Novozymes AS
Application granted granted Critical
Publication of DE69939829D1 publication Critical patent/DE69939829D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2451Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/2457Pullulanase (3.2.1.41)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2451Glucanases acting on alpha-1,6-glucosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2451Glucanases acting on alpha-1,6-glucosidic bonds
    • C12N9/246Isoamylase (3.2.1.68)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/16Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01041Pullulanase (3.2.1.41)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01068Isoamylase (3.2.1.68)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
DE69939829T 1998-07-02 1999-07-02 Stärke "debranching-enzyme" Expired - Lifetime DE69939829D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA199800868 1998-07-02
PCT/DK1999/000381 WO2000001796A2 (en) 1998-07-02 1999-07-02 Starch debranching enzymes

Publications (1)

Publication Number Publication Date
DE69939829D1 true DE69939829D1 (de) 2008-12-11

Family

ID=8098439

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69939829T Expired - Lifetime DE69939829D1 (de) 1998-07-02 1999-07-02 Stärke "debranching-enzyme"

Country Status (11)

Country Link
EP (2) EP2216401A1 (de)
JP (1) JP2002519054A (de)
KR (1) KR20010081985A (de)
CN (1) CN1309701A (de)
AT (1) ATE412739T1 (de)
AU (1) AU4897199A (de)
CA (1) CA2332697A1 (de)
DE (1) DE69939829D1 (de)
DK (1) DK1092014T3 (de)
MX (1) MXPA00012491A (de)
WO (1) WO2000001796A2 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6350599B1 (en) 2000-01-12 2002-02-26 Novozymes A/S Pullulanase variants and methods for preparing such variants with predetermined properties
US8765199B2 (en) 2006-06-15 2014-07-01 Novozymes A/S Mashing process
WO2008015861A1 (fr) * 2006-08-04 2008-02-07 Amano Enzyme Inc. Procédé de conception d'enzyme mutée, son procédé de préparation et enzyme mutée
US9969996B2 (en) 2006-08-04 2018-05-15 Amano Enzyme Inc. Method for making mutated pullulanase enzyme, mutated pullulanase enzyme, and microorganism expressing the same
US8021863B2 (en) 2007-02-19 2011-09-20 Novozymes A/S Polypeptides with starch debranching activity
MX2010006016A (es) * 2007-12-12 2010-07-01 Novozymes As Proceso de empastado de malta.
CN101952409B (zh) 2007-12-12 2014-12-10 诺维信公司 酿造方法
CN105623937A (zh) * 2007-12-12 2016-06-01 诺维信公司 糖化方法
CN102482658B (zh) * 2009-08-21 2015-07-15 诺维信公司 具有异淀粉酶活性的多肽和编码该多肽的多核苷酸
CN102791854A (zh) 2009-12-22 2012-11-21 诺维信公司 普鲁兰酶变体及其用途
EP2766490B1 (de) 2011-10-11 2017-04-12 Novozymes North America, Inc. Verflüssigungsverfahren zur herstellung von gärungsprodukten
CN102533701B (zh) * 2012-02-16 2015-01-14 江南大学 一种通过促进活性蛋白聚集体解聚提高淀粉脱支酶产量的方法
CN103484443B (zh) * 2012-07-23 2015-12-09 江南大学 一种普鲁兰酶突变体及其制备方法
CN102978191B (zh) * 2012-11-09 2015-07-22 江南大学 一种重组淀粉脱支酶发酵生产工艺
WO2015110473A2 (en) * 2014-01-22 2015-07-30 Novozymes A/S Pullulanase variants and polynucleotides encoding same
US10017751B2 (en) 2014-12-05 2018-07-10 Godo Shusei Co., Ltd. Heat-resistant isoamylase
US20190136277A1 (en) * 2015-12-31 2019-05-09 Cj Cheiljedang Corporation Method for preparing glucose from starch sugar by using acidothermus sp.-derived debranching enzyme having heat resistance and acid resistance, and glucose prepared thereby
WO2017204317A1 (ja) * 2016-05-27 2017-11-30 合同酒精株式会社 耐熱性イソアミラーゼ
CN107418988B (zh) * 2017-08-31 2021-02-19 华南理工大学 一种抗消化淀粉及其制备方法和应用
CN108546344A (zh) * 2018-04-20 2018-09-18 中国科学院长春应用化学研究所 一种淀粉胶的制备方法及淀粉基植物胶囊
CN108850837A (zh) * 2018-05-22 2018-11-23 贵州胖四娘食品有限公司 一种增强免疫力的保健粽子的生产方法
WO2020145288A1 (ja) * 2019-01-09 2020-07-16 合同酒精株式会社 耐熱性イソアミラーゼ
CN110184248B (zh) * 2019-05-27 2023-04-25 甘肃省商业科技研究所有限公司 一种嗜热淀粉分支酶诱导发酵的生产方法
CN111808836B (zh) * 2020-07-23 2021-12-07 中国农业科学院农产品加工研究所 耐热的i型普鲁兰酶的突变体酶及其制备方法与应用
CN114807099B (zh) * 2022-03-18 2024-05-03 浙江工业大学 普鲁兰酶突变体、工程菌及其应用
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN116064456B (zh) * 2022-11-28 2023-08-08 江南大学 一种低聚糖脱支酶突变体及其在葡萄糖母液中的应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0795012A1 (de) * 1994-03-04 1997-09-17 Novo Nordisk A/S AMYLASE UND PULLUANASE AUS $i(FERVIDOBACTERIUM)
EP0793716A1 (de) * 1994-03-04 1997-09-10 Novo Nordisk A/S AMYLASE UND PULLULANASE AUS $i(THERMOCOCCUS)
WO1995023853A1 (en) * 1994-03-04 1995-09-08 Novo Nordisk A/S Desulfurococcus amylase and pullulanase
WO1999035274A2 (en) * 1998-01-07 1999-07-15 Novo Nordisk A/S Nucleic acids encoding thermostable pullulanases
BR9909010A (pt) * 1998-03-23 2000-11-28 Novo Nordisk As Variante de fitase, polipeptìdeo de fitase, construção de dna, vetor recombinante de expressão, célula hospedeira, processos para preparar uma variante de fitase, ração ou alimento, para preparar uma ração ou alimento composição, e para reduzir os nìveis de fitato em estrume animal, uso da variante de fitase ou da composição, e planta transgênica ou parte de planta

Also Published As

Publication number Publication date
WO2000001796A3 (en) 2000-03-09
ATE412739T1 (de) 2008-11-15
DK1092014T3 (da) 2009-02-02
EP2216401A1 (de) 2010-08-11
CN1309701A (zh) 2001-08-22
EP1092014A2 (de) 2001-04-18
WO2000001796A2 (en) 2000-01-13
MXPA00012491A (es) 2002-07-02
KR20010081985A (ko) 2001-08-29
CA2332697A1 (en) 2000-01-13
EP1092014B1 (de) 2008-10-29
AU4897199A (en) 2000-01-24
JP2002519054A (ja) 2002-07-02

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