DE69633171T2 - Mutierte luziferasen - Google Patents
Mutierte luziferasen Download PDFInfo
- Publication number
- DE69633171T2 DE69633171T2 DE69633171T DE69633171T DE69633171T2 DE 69633171 T2 DE69633171 T2 DE 69633171T2 DE 69633171 T DE69633171 T DE 69633171T DE 69633171 T DE69633171 T DE 69633171T DE 69633171 T2 DE69633171 T2 DE 69633171T2
- Authority
- DE
- Germany
- Prior art keywords
- amino acid
- luciola
- luciferase
- protein
- residue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
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- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9501172.2A GB9501172D0 (en) | 1995-01-20 | 1995-01-20 | Luciferases |
| GB9501172 | 1995-01-20 | ||
| GB9508301 | 1995-04-24 | ||
| GBGB9508301.0A GB9508301D0 (en) | 1995-04-24 | 1995-04-24 | Luciferases |
| PCT/GB1996/000099 WO1996022376A1 (en) | 1995-01-20 | 1996-01-19 | Mutant luciferases |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69633171D1 DE69633171D1 (de) | 2004-09-23 |
| DE69633171T2 true DE69633171T2 (de) | 2005-08-18 |
Family
ID=26306352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69633171T Expired - Lifetime DE69633171T2 (de) | 1995-01-20 | 1996-01-19 | Mutierte luziferasen |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6171808B1 (en:Method) |
| EP (1) | EP0804587B1 (en:Method) |
| JP (1) | JP4342608B2 (en:Method) |
| AU (1) | AU707243B2 (en:Method) |
| CA (1) | CA2210354C (en:Method) |
| DE (1) | DE69633171T2 (en:Method) |
| DK (1) | DK0804587T3 (en:Method) |
| IN (1) | IN186115B (en:Method) |
| NO (1) | NO322134B1 (en:Method) |
| WO (1) | WO1996022376A1 (en:Method) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9707486D0 (en) * | 1997-04-11 | 1997-05-28 | Secr Defence | Enzyme assays |
| DE69841292D1 (de) * | 1997-07-08 | 2009-12-31 | Kikkoman Corp | Mutiertes Biolumineszenz-Protein und Verfahren zu dessen Herstellung |
| EP1721969B1 (en) * | 1997-07-08 | 2010-10-13 | Kikkoman Corporation | Mutant bioluminescent protein, and process for producing the mutant bioluminescent protein |
| CA2301828A1 (en) * | 1997-09-19 | 1999-03-25 | Promega Corporation | Thermostable luciferases and methods of production |
| US6602677B1 (en) | 1997-09-19 | 2003-08-05 | Promega Corporation | Thermostable luciferases and methods of production |
| GB9722481D0 (en) * | 1997-10-25 | 1997-12-24 | Secr Defence | Expression system |
| GB9823468D0 (en) | 1998-10-28 | 1998-12-23 | Secr Defence | Novel enzyme |
| JP5154724B2 (ja) * | 1999-10-26 | 2013-02-27 | プロメガ コーポレイション | 変異型ルシフェラーゼ |
| GB9925161D0 (en) | 1999-10-26 | 1999-12-22 | Secr Defence | Novel enzyme |
| US7879540B1 (en) | 2000-08-24 | 2011-02-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
| GB0030727D0 (en) | 2000-12-15 | 2001-01-31 | Lumitech Uk Ltd | Methods and kits for detecting kinase activity |
| GB0103622D0 (en) * | 2001-02-14 | 2001-03-28 | Univ Cambridge Tech | Methods for detecting DNA polymerisation |
| CN1304426C (zh) * | 2001-11-14 | 2007-03-14 | 英国国防部 | 信号系统及其使用的元件 |
| US7413874B2 (en) | 2002-12-09 | 2008-08-19 | University Of Miami | Nucleic acid encoding fluorescent proteins from aquatic species |
| GB2400659B8 (en) | 2003-04-17 | 2007-07-09 | Cambrex Bio Science Nottingham | Assay methods and materials |
| GB0416944D0 (en) | 2004-07-29 | 2004-09-01 | Lumora Ltd | Method for determining the amount of template nucleic acid present in a sample |
| US7728118B2 (en) | 2004-09-17 | 2010-06-01 | Promega Corporation | Synthetic nucleic acid molecule compositions and methods of preparation |
| JP5188771B2 (ja) | 2007-09-26 | 2013-04-24 | 株式会社日立製作所 | 変異型ホタルルシフェラーゼ |
| WO2009066673A1 (ja) * | 2007-11-20 | 2009-05-28 | Kikkoman Corporation | 核酸分析用組成物 |
| US8183007B2 (en) * | 2008-07-22 | 2012-05-22 | Promega Corporation | ADP detection based methods using adenylate cyclase and bioluminescence |
| JP2011120559A (ja) | 2008-12-26 | 2011-06-23 | Kikkoman Corp | ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法 |
| CA2817102C (en) | 2010-11-02 | 2020-07-28 | Promega Corporation | Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use |
| SG189494A1 (en) | 2010-11-02 | 2013-05-31 | Promega Corp | Coelenterazine derivatives and methods of using same |
| WO2014130760A1 (en) | 2013-02-22 | 2014-08-28 | Promega Corporation | Stabilized formulation for luminescent detection of luciferase and nucleoside phosphates |
| WO2015116806A1 (en) | 2014-01-29 | 2015-08-06 | Promega Corporation | Pro-substrates for live cell applications |
| EP3099683B1 (en) | 2014-01-29 | 2020-08-05 | Promega Corporation | Quinone-masked probes as labeling reagents for cell uptake measurements |
| WO2016040788A1 (en) | 2014-09-11 | 2016-03-17 | Promega Corporation | Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence |
| US9677117B2 (en) | 2014-10-08 | 2017-06-13 | Promega Corporation | Bioluminescent succinate detection assay |
| DE102015215569A1 (de) | 2015-08-14 | 2017-02-16 | Robert Bosch Gmbh | Verfahren zur Generierung eines Geheimnisses zwischen Teilnehmern eines Netzwerkes sowie dazu eingerichtete Teilnehmer des Netzwerks |
| JPWO2021162123A1 (en:Method) | 2020-02-14 | 2021-08-19 | ||
| JPWO2024019165A1 (en:Method) | 2022-07-22 | 2024-01-25 | ||
| JPWO2024158050A1 (en:Method) | 2023-01-27 | 2024-08-02 | ||
| CN119931968A (zh) * | 2024-05-10 | 2025-05-06 | 中国人民解放军军事科学院军事医学研究院 | 一种荧光素酶突变体及其应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5219737A (en) | 1990-03-27 | 1993-06-15 | Kikkoman Corporation | Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase |
| US5229285A (en) | 1991-06-27 | 1993-07-20 | Kikkoman Corporation | Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly |
| DE4123672A1 (de) | 1991-07-17 | 1993-01-21 | Basf Ag | Siegelfaehige, peelbare kunststoffolie |
| ATE391769T1 (de) * | 1994-01-03 | 2008-04-15 | Promega Corp | Mutierte luziferasen |
| GB9501170D0 (en) * | 1994-03-23 | 1995-03-08 | Secr Defence | Luciferases |
-
1996
- 1996-01-19 DE DE69633171T patent/DE69633171T2/de not_active Expired - Lifetime
- 1996-01-19 EP EP96900380A patent/EP0804587B1/en not_active Expired - Lifetime
- 1996-01-19 IN IN122DE1996 patent/IN186115B/en unknown
- 1996-01-19 US US08/875,277 patent/US6171808B1/en not_active Expired - Lifetime
- 1996-01-19 JP JP52211996A patent/JP4342608B2/ja not_active Expired - Lifetime
- 1996-01-19 AU AU43973/96A patent/AU707243B2/en not_active Ceased
- 1996-01-19 CA CA002210354A patent/CA2210354C/en not_active Expired - Fee Related
- 1996-01-19 WO PCT/GB1996/000099 patent/WO1996022376A1/en active IP Right Grant
- 1996-01-19 DK DK96900380T patent/DK0804587T3/da active
-
1997
- 1997-07-18 NO NO19973349A patent/NO322134B1/no not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| AU4397396A (en) | 1996-08-07 |
| DK0804587T3 (da) | 2004-10-11 |
| NO973349L (no) | 1997-09-19 |
| CA2210354C (en) | 2008-01-08 |
| JPH10512750A (ja) | 1998-12-08 |
| IN186115B (en:Method) | 2001-06-23 |
| WO1996022376A1 (en) | 1996-07-25 |
| CA2210354A1 (en) | 1996-07-25 |
| US6171808B1 (en) | 2001-01-09 |
| DE69633171D1 (de) | 2004-09-23 |
| AU707243B2 (en) | 1999-07-08 |
| EP0804587A1 (en) | 1997-11-05 |
| NO973349D0 (no) | 1997-07-18 |
| JP4342608B2 (ja) | 2009-10-14 |
| EP0804587B1 (en) | 2004-08-18 |
| NO322134B1 (no) | 2006-08-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: 3M INNOVATIVE PROPERTIES CO., ST. PAUL, MINN., US |
|
| 8327 | Change in the person/name/address of the patent owner |
Owner name: PROMEGA CORP., MADISON, WIS., US |