DE69534941D1 - Herstellung einer multikombinatorischen bank von antikörpergene-exprimierende vektoren - Google Patents

Herstellung einer multikombinatorischen bank von antikörpergene-exprimierende vektoren

Info

Publication number
DE69534941D1
DE69534941D1 DE69534941T DE69534941T DE69534941D1 DE 69534941 D1 DE69534941 D1 DE 69534941D1 DE 69534941 T DE69534941 T DE 69534941T DE 69534941 T DE69534941 T DE 69534941T DE 69534941 D1 DE69534941 D1 DE 69534941D1
Authority
DE
Germany
Prior art keywords
antibody
repertoire
vector
genes
multicomponent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69534941T
Other languages
English (en)
Other versions
DE69534941T2 (de
Inventor
Regis Sodoyer
Luc Aujame
Frederique Geoffroy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sanofi Pasteur SA
Original Assignee
Sanofi Pasteur SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Pasteur SA filed Critical Sanofi Pasteur SA
Application granted granted Critical
Publication of DE69534941D1 publication Critical patent/DE69534941D1/de
Publication of DE69534941T2 publication Critical patent/DE69534941T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1036—Retroviridae, e.g. leukemia viruses
    • C07K16/1045—Lentiviridae, e.g. HIV, FIV, SIV
    • C07K16/1063—Lentiviridae, e.g. HIV, FIV, SIV env, e.g. gp41, gp110/120, gp160, V3, PND, CD4 binding site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • AIDS & HIV (AREA)
  • Hematology (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
DE69534941T 1994-02-10 1995-02-02 Herstellung einer multikombinatorischen bank von antikörpergene-exprimierende vektoren Expired - Lifetime DE69534941T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9401519 1994-02-10
FR9401519A FR2715940B1 (fr) 1994-02-10 1994-02-10 Procédé de préparation d'une banque multicombinatoire de vecteurs d'expression de gènes d'anticorps, banque et systèmes d'expression d'anticorps "coliclonaux" obtenus.
PCT/FR1995/000127 WO1995021914A1 (fr) 1994-02-10 1995-02-02 Procede de preparation d'une banque multicombinatoire de vecteurs d'expression de genes d'anticorps

Publications (2)

Publication Number Publication Date
DE69534941D1 true DE69534941D1 (de) 2006-05-24
DE69534941T2 DE69534941T2 (de) 2006-09-28

Family

ID=9459966

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69534941T Expired - Lifetime DE69534941T2 (de) 1994-02-10 1995-02-02 Herstellung einer multikombinatorischen bank von antikörpergene-exprimierende vektoren

Country Status (11)

Country Link
US (1) US6599697B1 (de)
EP (1) EP0742821B1 (de)
AT (1) ATE323762T1 (de)
AU (1) AU1666895A (de)
CA (1) CA2183079C (de)
DE (1) DE69534941T2 (de)
DK (1) DK0742821T3 (de)
ES (1) ES2257740T3 (de)
FR (1) FR2715940B1 (de)
PT (1) PT742821E (de)
WO (1) WO1995021914A1 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2731014B1 (fr) * 1995-02-23 1997-03-28 Rhone Poulenc Rorer Sa Molecules d'adn, preparation et utilisation en therapie genique
FR2741892B1 (fr) 1995-12-04 1998-02-13 Pasteur Merieux Serums Vacc Procede de preparation d'une banque multicombinatoire de vecteurs d'expression de genes d'anticorps, banque et systemes d'expression d'anticorps "coliclonaux" obtenus
ATE239077T1 (de) * 1997-01-31 2003-05-15 Cosmix Molecular Biolog Gmbh Erzeugung von diversität in kombinatorischen banken
US6310191B1 (en) 1998-02-02 2001-10-30 Cosmix Molecular Biologicals Gmbh Generation of diversity in combinatorial libraries
WO1999006587A2 (en) 1997-08-01 1999-02-11 Morphosys Ag Novel method and phage for the identification of nucleic acid sequences encoding members of a multimeric (poly)peptide complex
JP4303418B2 (ja) * 1997-10-24 2009-07-29 ライフ テクノロジーズ コーポレーション 組換え部位を有する核酸を使用する組換えクローニング
EP1194540A1 (de) * 1999-07-14 2002-04-10 Clontech Laboratories Inc. Rekombinase-basierende methoden zur herstellung von expressionsvektoren und zusammensetzungen für ihre verwendung
GB2375112A (en) * 2000-02-03 2002-11-06 Domantis Ltd Combinatorial protein domains
WO2002012277A2 (en) * 2000-08-07 2002-02-14 Domantis Limited Hybrid combinatorial proteins made from reshuffling of differently folded domains
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6610472B1 (en) * 2000-10-31 2003-08-26 Genetastix Corporation Assembly and screening of highly complex and fully human antibody repertoire in yeast
US20050196755A1 (en) * 2000-11-17 2005-09-08 Maurice Zauderer In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells
JP4368196B2 (ja) * 2000-11-17 2009-11-18 ユニバーシティー オブ ロチェスター 真核細胞において免疫グロブリン分子を製造および同定するインビトロにおける方法
US20020164703A1 (en) * 2000-12-21 2002-11-07 Krzysztof Pawlowski Card-domain containing polypeptides, encoding nucleic acids, and methods of use
WO2002086096A2 (en) * 2001-01-23 2002-10-31 University Of Rochester Medical Center Methods of producing or identifying intrabodies in eukaryotic cells
EP1697534B1 (de) 2003-12-01 2010-06-02 Life Technologies Corporation Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
US8921281B2 (en) 2009-05-20 2014-12-30 Novimmune S.A. Synthetic polypeptide libraries and methods for generating naturally diversified polypeptide variants
US9617329B2 (en) 2010-10-01 2017-04-11 Csl Limited Method of cloning nucleic acid
CA2989143A1 (en) 2015-06-12 2016-12-15 Axiomx, Inc. Methods and compositions for producing a chimeric polypeptide

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4673640A (en) * 1984-04-30 1987-06-16 Biotechnica International, Inc. Regulated protein production using site-specific recombination
WO1992020791A1 (en) * 1990-07-10 1992-11-26 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
EP0656941B1 (de) * 1992-03-24 2005-06-01 Cambridge Antibody Technology Limited Verfahren zur herstellung von gliedern von spezifischen bindungspaaren
FR2741892B1 (fr) * 1995-12-04 1998-02-13 Pasteur Merieux Serums Vacc Procede de preparation d'une banque multicombinatoire de vecteurs d'expression de genes d'anticorps, banque et systemes d'expression d'anticorps "coliclonaux" obtenus

Also Published As

Publication number Publication date
PT742821E (pt) 2006-06-30
JP3994291B2 (ja) 2007-10-17
DK0742821T3 (da) 2006-08-21
ATE323762T1 (de) 2006-05-15
FR2715940B1 (fr) 1996-04-26
DE69534941T2 (de) 2006-09-28
CA2183079A1 (fr) 1995-08-17
EP0742821B1 (de) 2006-04-19
AU1666895A (en) 1995-08-29
FR2715940A1 (fr) 1995-08-11
EP0742821A1 (de) 1996-11-20
US6599697B1 (en) 2003-07-29
JPH09509055A (ja) 1997-09-16
WO1995021914A1 (fr) 1995-08-17
ES2257740T3 (es) 2006-08-01
CA2183079C (fr) 2007-08-07

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Legal Events

Date Code Title Description
8328 Change in the person/name/address of the agent

Representative=s name: PFENNING MEINIG & PARTNER GBR, 80339 MUENCHEN

8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: MAIWALD PATENTANWALTSGESELLSCHAFT MBH, 80335 MUENC