ATE323762T1 - Herstellung einer multikombinatorischen bank von antikörpergene-exprimierende vektoren - Google Patents

Herstellung einer multikombinatorischen bank von antikörpergene-exprimierende vektoren

Info

Publication number
ATE323762T1
ATE323762T1 AT95908302T AT95908302T ATE323762T1 AT E323762 T1 ATE323762 T1 AT E323762T1 AT 95908302 T AT95908302 T AT 95908302T AT 95908302 T AT95908302 T AT 95908302T AT E323762 T1 ATE323762 T1 AT E323762T1
Authority
AT
Austria
Prior art keywords
multicombinatory
bank
preparation
repertoire
vector
Prior art date
Application number
AT95908302T
Other languages
English (en)
Inventor
Regis Sodoyer
Luc Aujame
Frederique Geoffroy
Original Assignee
Sanofi Pasteur
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sanofi Pasteur filed Critical Sanofi Pasteur
Application granted granted Critical
Publication of ATE323762T1 publication Critical patent/ATE323762T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—RNA viruses
    • C07K16/112—Retroviridae (F), e.g. leukemia viruses
    • C07K16/114—Lentivirus (G), e.g. human immunodeficiency virus [HIV], feline immunodeficiency virus [FIV] or simian immunodeficiency virus [SIV]
    • C07K16/1145—Env proteins, e.g. gp41, gp110/120, gp160, V3, principal neutralising domain [PND] or CD4-binding site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Management, Administration, Business Operations System, And Electronic Commerce (AREA)
AT95908302T 1994-02-10 1995-02-02 Herstellung einer multikombinatorischen bank von antikörpergene-exprimierende vektoren ATE323762T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9401519A FR2715940B1 (fr) 1994-02-10 1994-02-10 Procédé de préparation d'une banque multicombinatoire de vecteurs d'expression de gènes d'anticorps, banque et systèmes d'expression d'anticorps "coliclonaux" obtenus.

Publications (1)

Publication Number Publication Date
ATE323762T1 true ATE323762T1 (de) 2006-05-15

Family

ID=9459966

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95908302T ATE323762T1 (de) 1994-02-10 1995-02-02 Herstellung einer multikombinatorischen bank von antikörpergene-exprimierende vektoren

Country Status (11)

Country Link
US (1) US6599697B1 (de)
EP (1) EP0742821B1 (de)
AT (1) ATE323762T1 (de)
AU (1) AU1666895A (de)
CA (1) CA2183079C (de)
DE (1) DE69534941T2 (de)
DK (1) DK0742821T3 (de)
ES (1) ES2257740T3 (de)
FR (1) FR2715940B1 (de)
PT (1) PT742821E (de)
WO (1) WO1995021914A1 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2731014B1 (fr) * 1995-02-23 1997-03-28 Rhone Poulenc Rorer Sa Molecules d'adn, preparation et utilisation en therapie genique
FR2741892B1 (fr) * 1995-12-04 1998-02-13 Pasteur Merieux Serums Vacc Procede de preparation d'une banque multicombinatoire de vecteurs d'expression de genes d'anticorps, banque et systemes d'expression d'anticorps "coliclonaux" obtenus
CN1246150A (zh) * 1997-01-31 2000-03-01 科斯米克斯分子生物有限公司 组合文库的多样性的产生
US6310191B1 (en) 1998-02-02 2001-10-30 Cosmix Molecular Biologicals Gmbh Generation of diversity in combinatorial libraries
WO1999006587A2 (en) 1997-08-01 1999-02-11 Morphosys Ag Novel method and phage for the identification of nucleic acid sequences encoding members of a multimeric (poly)peptide complex
DE69836092T2 (de) * 1997-10-24 2007-05-10 Invitrogen Corp., Carlsbad Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen
US6410317B1 (en) * 1999-07-14 2002-06-25 Clontech Laboratories, Inc. Recombinase-based methods for producing expression vectors and compositions for use in practicing the same
WO2001057065A2 (en) * 2000-02-03 2001-08-09 Domantis Limited Combinatorial protein domains
AU2001277614A1 (en) * 2000-08-07 2002-02-18 Domantis Limited Novel proteins
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6610472B1 (en) * 2000-10-31 2003-08-26 Genetastix Corporation Assembly and screening of highly complex and fully human antibody repertoire in yeast
US20050196755A1 (en) * 2000-11-17 2005-09-08 Maurice Zauderer In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells
CN1306272C (zh) * 2000-11-17 2007-03-21 罗切斯特大学 筛选编码抗原特异性免疫球蛋白分子或其抗原特异性片段的方法
US20020164703A1 (en) * 2000-12-21 2002-11-07 Krzysztof Pawlowski Card-domain containing polypeptides, encoding nucleic acids, and methods of use
US20030104402A1 (en) * 2001-01-23 2003-06-05 University Of Rochester Methods of producing or identifying intrabodies in eukaryotic cells
DE602004027538D1 (de) 2003-12-01 2010-07-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
AU2010249470B2 (en) * 2009-05-20 2015-06-25 Novimmune S.A. Synthetic Polypeptide Libraries And Methods For Generating Naturally Diversified Polypeptide Variants
US9617329B2 (en) 2010-10-01 2017-04-11 Csl Limited Method of cloning nucleic acid
CN113307864A (zh) 2015-06-12 2021-08-27 艾希奥美公司 用于产生嵌合多肽的方法和组合物

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4673640A (en) * 1984-04-30 1987-06-16 Biotechnica International, Inc. Regulated protein production using site-specific recombination
ES2139598T3 (es) * 1990-07-10 2000-02-16 Medical Res Council Procedimientos para la produccion de miembros de parejas de union especifica.
EP0656941B1 (de) * 1992-03-24 2005-06-01 Cambridge Antibody Technology Limited Verfahren zur herstellung von gliedern von spezifischen bindungspaaren
FR2741892B1 (fr) * 1995-12-04 1998-02-13 Pasteur Merieux Serums Vacc Procede de preparation d'une banque multicombinatoire de vecteurs d'expression de genes d'anticorps, banque et systemes d'expression d'anticorps "coliclonaux" obtenus

Also Published As

Publication number Publication date
WO1995021914A1 (fr) 1995-08-17
US6599697B1 (en) 2003-07-29
EP0742821B1 (de) 2006-04-19
JPH09509055A (ja) 1997-09-16
AU1666895A (en) 1995-08-29
ES2257740T3 (es) 2006-08-01
DK0742821T3 (da) 2006-08-21
CA2183079A1 (fr) 1995-08-17
EP0742821A1 (de) 1996-11-20
JP3994291B2 (ja) 2007-10-17
FR2715940B1 (fr) 1996-04-26
DE69534941T2 (de) 2006-09-28
PT742821E (pt) 2006-06-30
CA2183079C (fr) 2007-08-07
FR2715940A1 (fr) 1995-08-11
DE69534941D1 (de) 2006-05-24

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