DE69531545D1 - Photolabile verbindungen und verfahren für deren verwendung - Google Patents

Photolabile verbindungen und verfahren für deren verwendung

Info

Publication number
DE69531545D1
DE69531545D1 DE69531545T DE69531545T DE69531545D1 DE 69531545 D1 DE69531545 D1 DE 69531545D1 DE 69531545 T DE69531545 T DE 69531545T DE 69531545 T DE69531545 T DE 69531545T DE 69531545 D1 DE69531545 D1 DE 69531545D1
Authority
DE
Germany
Prior art keywords
photolabile
connections
photolabile connections
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69531545T
Other languages
English (en)
Other versions
DE69531545T2 (de
Inventor
Christopher Holmes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Affymax Technologies NV
Original Assignee
Affymax Technologies NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/265,090 external-priority patent/US5549974A/en
Priority claimed from US08/374,492 external-priority patent/US5679773A/en
Application filed by Affymax Technologies NV filed Critical Affymax Technologies NV
Application granted granted Critical
Publication of DE69531545D1 publication Critical patent/DE69531545D1/de
Publication of DE69531545T2 publication Critical patent/DE69531545T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D403/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00
    • C07D403/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings
    • C07D403/04—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C233/00—Carboxylic acid amides
    • C07C233/01—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms
    • C07C233/16—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms
    • C07C233/17—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom
    • C07C233/18—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by singly-bound oxygen atoms with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom having the carbon atom of the carboxamide group bound to a hydrogen atom or to a carbon atom of an acyclic saturated carbon skeleton
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/02—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings
    • C07D277/08—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member
    • C07D277/12—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having one double bond between ring members or between a ring member and a non-ring member with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D277/14—Oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/02—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings
    • C07D277/20—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
    • C07D277/32—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D277/34—Oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D279/00—Heterocyclic compounds containing six-membered rings having one nitrogen atom and one sulfur atom as the only ring hetero atoms
    • C07D279/04—1,3-Thiazines; Hydrogenated 1,3-thiazines
    • C07D279/06—1,3-Thiazines; Hydrogenated 1,3-thiazines not condensed with other rings
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D317/00—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms
    • C07D317/08—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms having the hetero atoms in positions 1 and 3
    • C07D317/10—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms having the hetero atoms in positions 1 and 3 not condensed with other rings
    • C07D317/14—Heterocyclic compounds containing five-membered rings having two oxygen atoms as the only ring hetero atoms having the hetero atoms in positions 1 and 3 not condensed with other rings with substituted hydrocarbon radicals attached to ring carbon atoms
    • C07D317/28—Radicals substituted by nitrogen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/006—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length of peptides containing derivatised side chain amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/06—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents
    • C07K1/08—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using activating agents
    • C07K1/084—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using activating agents containing nitrogen
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06K—GRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
    • G06K19/00—Record carriers for use with machines and with at least a part designed to carry digital markings
    • G06K19/06—Record carriers for use with machines and with at least a part designed to carry digital markings characterised by the kind of the digital marking, e.g. shape, nature, code
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00709—Type of synthesis
    • B01J2219/00711—Light-directed synthesis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00725—Peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06K—GRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
    • G06K19/00—Record carriers for use with machines and with at least a part designed to carry digital markings
    • G06K19/06—Record carriers for use with machines and with at least a part designed to carry digital markings characterised by the kind of the digital marking, e.g. shape, nature, code
    • G06K2019/06215—Aspects not covered by other subgroups
    • G06K2019/06234—Aspects not covered by other subgroups miniature-code
DE69531545T 1994-06-23 1995-06-23 Photolabile verbindungen und verfahren für deren verwendung Expired - Fee Related DE69531545T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/265,090 US5549974A (en) 1994-06-23 1994-06-23 Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof
US08/374,492 US5679773A (en) 1995-01-17 1995-01-17 Reagants and methods for immobilized polymer synthesis and display
US374492 1995-01-17
PCT/US1995/007985 WO1996000378A1 (en) 1994-06-23 1995-06-23 Photolabile compounds and methods for their use
US265090 2002-10-04

Publications (2)

Publication Number Publication Date
DE69531545D1 true DE69531545D1 (de) 2003-09-25
DE69531545T2 DE69531545T2 (de) 2004-06-24

Family

ID=26950978

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69531545T Expired - Fee Related DE69531545T2 (de) 1994-06-23 1995-06-23 Photolabile verbindungen und verfahren für deren verwendung

Country Status (7)

Country Link
US (2) US5739386A (de)
EP (1) EP0776330B1 (de)
JP (1) JPH10507160A (de)
AU (1) AU689924B2 (de)
CA (1) CA2193228A1 (de)
DE (1) DE69531545T2 (de)
WO (1) WO1996000378A1 (de)

Families Citing this family (127)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6589726B1 (en) 1991-09-04 2003-07-08 Metrigen, Inc. Method and apparatus for in situ synthesis on a solid support
US6312893B1 (en) 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
US6027890A (en) 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US6613508B1 (en) 1996-01-23 2003-09-02 Qiagen Genomics, Inc. Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques
AU2411197A (en) * 1996-04-30 1997-11-19 Affymax Technologies N.V. Methods for the synthesis of fmoc protected amines
GB9621985D0 (en) 1996-10-22 1996-12-18 Peptide Therapeutics Ltd A solid-phase technology for the preparation of libraries of bi-directally functionalised drug-like molecules
US6281245B1 (en) 1996-10-28 2001-08-28 Versicor, Inc. Methods for solid-phase synthesis of hydroxylamine compounds and derivatives, and combinatorial libraries thereof
AU5426398A (en) 1996-10-28 1998-05-22 Versicor Inc Methods for solid-phase synthesis of hydroxylamine compounds and derivatives, and combinatorial libraries thereof
US6410342B1 (en) * 1997-08-19 2002-06-25 Pharmacopeia, Inc. Method and apparatus for controlled photoelution
US6511803B1 (en) * 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
CA2311490A1 (en) * 1997-11-26 1999-06-03 Tony Johnson A solid-phase technology for the preparation of combinatorial libraries through amide-bond anchoring
CA2319587C (en) * 1998-02-11 2004-09-21 University Of Houston Method and apparatus for chemical and biochemical reactions using photo-generated reagents
US6310244B1 (en) * 1998-07-23 2001-10-30 Pharmacopeia, Inc. Photocleavable and acid cleavable linkers for combinatorial chemistry
AUPP616598A0 (en) 1998-09-25 1998-10-15 University Of Queensland, The Auxiliary for amide bond formation
AUPP616498A0 (en) 1998-09-25 1998-10-15 University Of Queensland, The Synthesis of cyclic peptides
JP2002538790A (ja) * 1999-03-08 2002-11-19 プロトジーン・ラボラトリーズ・インコーポレーテッド 長いｄｎａ配列を経済的に合成し、そして組み立てるための方法および組成物
US6713309B1 (en) 1999-07-30 2004-03-30 Large Scale Proteomics Corporation Microarrays and their manufacture
US7179638B2 (en) 1999-07-30 2007-02-20 Large Scale Biology Corporation Microarrays and their manufacture by slicing
US6653151B2 (en) 1999-07-30 2003-11-25 Large Scale Proteomics Corporation Dry deposition of materials for microarrays using matrix displacement
EP1235932A2 (de) 1999-10-08 2002-09-04 Protogene Laboratories, Inc. Verfahren und vorrichtung zur durchführung von hohen zahlen von reaktionen unter benutzung von arrays
US7157458B2 (en) 2001-04-17 2007-01-02 Cryolife, Inc. Bifunctional energy-reversible acyl-compositions
US7183116B2 (en) 2001-05-14 2007-02-27 The Institute For Systems Biology Methods for isolation and labeling of sample molecules
EP1401850A1 (de) 2001-06-20 2004-03-31 Nuevolution A/S Nukleosidderivate zur herstellung einer verbindungsbibliothek
US8569516B2 (en) * 2001-09-07 2013-10-29 Elitech Holding B.V. Compounds and methods for fluorescent labeling
US6972339B2 (en) 2001-09-07 2005-12-06 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
WO2003078625A2 (en) * 2002-03-15 2003-09-25 Nuevolution A/S An improved method for synthesising templated molecules
AU2003247266A1 (en) 2002-08-01 2004-02-23 Nuevolution A/S Multi-step synthesis of templated molecules
US7473535B2 (en) * 2002-08-20 2009-01-06 The Institute For Systems Biology Chemical reagents and methods for detection and quantification of proteins in complex mixtures
ES2666694T3 (es) * 2002-09-06 2018-05-07 Cerulean Pharma Inc. Polímeros a base de ciclodextrina para el suministro de los agentes terapéuticos enlazados covalentemente a ellos
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
CA2539890A1 (en) * 2002-09-27 2004-04-08 Carlsberg A/S Spatially encoded polymer matrix
CN106337046B (zh) 2002-10-30 2021-03-23 纽韦卢森公司 合成双功能复合物的方法
DE60330406D1 (de) 2002-12-19 2010-01-14 Nuevolution As Durch quasizufallsstrukturen und funktionen geführte synthesemethode
WO2004068139A2 (en) * 2003-01-10 2004-08-12 The Regents Of The University Of California Improvement of catalytic efficiency and/or specificity of non-native substrates of enzymes
WO2004074429A2 (en) 2003-02-21 2004-09-02 Nuevolution A/S Method for producing second-generation library
EP2236606B1 (de) 2003-03-20 2013-11-06 Nuevolution A/S Ligationsvermittelnde Codierung von kleinen Molekülen
DE602004023960D1 (de) 2003-09-18 2009-12-17 Nuevolution As Methode zur Gewinnung struktureller Informationen kodierter Moleküle und zur Selektion von Verbindungen
US7541454B2 (en) * 2003-10-24 2009-06-02 Epoch Biosciences, Inc. Compounds and methods for fluorescent labeling
US20090239211A1 (en) * 2004-02-17 2009-09-24 Nuevolution A/S Method For Enrichment Involving Elimination By Mismatch Hybridisation
ATE447020T1 (de) 2004-03-22 2009-11-15 Nuevolution As Ligationscodierung unter verwendung von oligonukleotidbausteinen
WO2005108417A1 (ja) * 2004-05-07 2005-11-17 National Institute Of Advanced Industrial Science And Technology 糖ぺプチド合成用高分子プライマー
US20060141528A1 (en) 2004-05-21 2006-06-29 Aebersold Rudolf H Compositions and methods for quantification of serum glycoproteins
EP1828780B1 (de) 2004-12-22 2011-11-23 California Institute Of Technology Verfahren zur proteomischen profilierung mithilfe nichtnatürlicher aminosäuren
US8343710B1 (en) * 2005-03-11 2013-01-01 The Regents Of The University Of Colorado, A Body Corporate Photodegradable groups for tunable polymeric materials
US9180196B2 (en) 2005-03-11 2015-11-10 The Regents Of The University Of Colorado, A Body Corporate Photodegradable groups for tunable polymeric materials
ES2382876T3 (es) * 2005-05-31 2012-06-14 Pfizer, Inc. Compuestos de ariloxi-N-biciclometil-acetamida sustituidos como antagonistas de VR1
US20080312092A1 (en) * 2005-07-08 2008-12-18 The University Of Denver Photoinduced Signal Amplification Through Externally Sensitized Photofragmentation in Masked Photosensitizers
WO2007021016A1 (ja) * 2005-08-19 2007-02-22 Shimadzu Corporation 光制御ペプチド及び光制御ペプチドを用いたペプチド－蛋白質複合体形成の制御方法
ES2897529T3 (es) 2005-12-01 2022-03-01 Nuevolution As Métodos de codificación enzimática para la síntesis eficiente de bibliotecas grandes
WO2007070761A2 (en) * 2005-12-12 2007-06-21 Colorado Seminary, Which Owns And Operates The University Of Denver Method for encoding and screening combinatorial libraries
WO2008008481A2 (en) * 2006-07-12 2008-01-17 Georgia Tech Research Corporation Deprotection of functional groups by multi-photon induced electron transfer
WO2008027558A2 (en) 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
WO2008045380A2 (en) * 2006-10-04 2008-04-17 Codon Devices, Inc. Nucleic acid libraries and their design and assembly
US20080176958A1 (en) 2007-01-24 2008-07-24 Insert Therapeutics, Inc. Cyclodextrin-based polymers for therapeutics delivery
WO2009026313A1 (en) * 2007-08-20 2009-02-26 Colorado Seminary, Which Owns And Operates The University Of Denver Photoinduced signal amplification through externally sensitized photofragmentation in masked photosensitizers and photoamplified fluorescence turn-off system
DE102007062154A1 (de) * 2007-12-21 2009-06-25 Emc Microcollections Gmbh Verfahren zur Herstellung und Anwendung von stochastisch angeordneten Arrays von Testsubstanzen
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
CN104208716A (zh) * 2009-11-23 2014-12-17 天蓝制药公司 用于传递治疗剂的基于环糊精的聚合物
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US8951940B2 (en) 2010-04-01 2015-02-10 Illumina, Inc. Solid-phase clonal amplification and related methods
WO2011127933A1 (en) 2010-04-16 2011-10-20 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
WO2011143556A1 (en) 2010-05-13 2011-11-17 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
WO2011150168A1 (en) 2010-05-28 2011-12-01 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
US8865404B2 (en) 2010-11-05 2014-10-21 President And Fellows Of Harvard College Methods for sequencing nucleic acid molecules
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
LT3360963T (lt) 2010-11-12 2020-02-25 Gen9, Inc. Nukleorūgščių sintezės būdai ir įrenginiai
US20140017797A1 (en) 2010-12-13 2014-01-16 De Staadt der Nederlanden, vert doorde minister van VWS Protein affinity tag and uses thereof
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
US9056887B2 (en) 2011-07-26 2015-06-16 Elitech Holding B.V. Minor groove binder phosphoramidites and methods of use
US20130059296A1 (en) 2011-08-26 2013-03-07 Gen9, Inc. Compositions and Methods For High Fidelity Assembly of Nucleic Acids
JP2014531908A (ja) 2011-10-14 2014-12-04 プレジデント アンド フェローズ オブ ハーバード カレッジ 構造アッセンブリによる配列決定
WO2013057188A1 (en) 2011-10-19 2013-04-25 Danmarks Tekniske Universitet In-bead screening
WO2013057186A1 (en) 2011-10-19 2013-04-25 Danmarks Tekniske Universitet Photolabile linker for the synthesis of hydroxamic acids
WO2013123125A1 (en) 2012-02-17 2013-08-22 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US10081807B2 (en) 2012-04-24 2018-09-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
LT2864531T (lt) 2012-06-25 2019-03-12 Gen9, Inc. Nukleorūgšties konstravimo ir aukšto produktyvumo sekvenavimo būdai
WO2014055493A1 (en) 2012-10-02 2014-04-10 Cerulean Pharma Inc. Methods and systems for polymer precipitation and generation of particles
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
EP3030682B1 (de) 2013-08-05 2020-06-03 Twist Bioscience Corporation De-novo-synthetisierte genbanken
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
CN108368482A (zh) 2015-09-18 2018-08-03 特韦斯特生物科学公司 寡核酸变体文库及其合成
KR20180058772A (ko) 2015-09-22 2018-06-01 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
US9895673B2 (en) 2015-12-01 2018-02-20 Twist Bioscience Corporation Functionalized surfaces and preparation thereof
EP3500672A4 (de) 2016-08-22 2020-05-20 Twist Bioscience Corporation De-novo-synthetisierte nukleinsäure-bibliotheken
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
EP3595674A4 (de) 2017-03-15 2020-12-16 Twist Bioscience Corporation Variante bibliotheken der immunologischen synapse und synthese davon
KR102628876B1 (ko) 2017-06-12 2024-01-23 트위스트 바이오사이언스 코포레이션 심리스 핵산 어셈블리를 위한 방법
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
SG11202002194UA (en) 2017-09-11 2020-04-29 Twist Bioscience Corp Gpcr binding proteins and synthesis thereof
JP7066840B2 (ja) 2017-10-20 2022-05-13 ツイスト バイオサイエンス コーポレーション ポリヌクレオチド合成のための加熱されたナノウェル
CN112041438A (zh) 2018-01-04 2020-12-04 特韦斯特生物科学公司 基于dna的数字信息存储
SG11202011467RA (en) 2018-05-18 2020-12-30 Twist Bioscience Corp Polynucleotides, reagents, and methods for nucleic acid hybridization
AU2019311199A1 (en) 2018-07-23 2021-01-28 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
AU2019379287A1 (en) 2018-11-14 2021-06-03 Dna Script Terminal deoxynucleotidyl transferase variants and uses thereof
WO2020141143A1 (en) 2019-01-03 2020-07-09 Dna Script One pot synthesis of sets of oligonucleotides
KR20210143766A (ko) 2019-02-26 2021-11-29 트위스트 바이오사이언스 코포레이션 Glp1 수용체에 대한 변이체 핵산 라이브러리
AU2020227802A1 (en) 2019-02-26 2021-10-14 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
CN114729342A (zh) 2019-06-21 2022-07-08 特韦斯特生物科学公司 基于条形码的核酸序列装配
AU2020319829A1 (en) 2019-07-30 2022-02-24 Dna Script Template-free enzymatic synthesis of polynucleotides using poly(A) and poly(U) polymerases
WO2021018921A1 (en) 2019-08-01 2021-02-04 Dna Script Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides.
CA3151411A1 (en) 2019-09-23 2021-04-01 Adrian Horgan Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides
WO2021086982A2 (en) 2019-10-28 2021-05-06 Beckman Coulter, Inc. Compounds for the identification of microbial classes and uses thereof
EP4077703A1 (de) 2019-12-16 2022-10-26 DNA Script Schablonenfreie enzymatische polynukleotidsynthese unter verwendung von dismutationsfreien varianten der terminalen deoxynukleotidyl-transferase
WO2021170524A1 (en) 2020-02-25 2021-09-02 Dna Script Method and apparatus for enzymatic synthesis of polynucleotides
WO2021213903A1 (en) 2020-04-20 2021-10-28 Dna Script Terminal deoxynucleotidyl transferase variants and uses thereof
KR20230024399A (ko) 2020-06-16 2023-02-20 디엔에이 스크립트 효소적 폴리뉴클레오티드 합성을 위한 시스템, 장치 및 키트
US20230313255A1 (en) 2020-07-15 2023-10-05 Dna Script Massively Parallel Enzymatic Synthesis of Polynucleotides
US20240093256A1 (en) 2020-09-22 2024-03-21 Dna Script Stabilized N-Terminally Truncated Terminal Deoxynucleotidyl Transferase Variants and Uses Thereof
JP2024511874A (ja) 2021-04-02 2024-03-15 ディーエヌエー スクリプト Ｇ４を形成しやすいポリヌクレオチドの酵素合成のための方法及びキット
CN113504371A (zh) * 2021-07-09 2021-10-15 博以新(杭州)生物技术有限公司 基于环糊精包合的免分离化学发光免疫分析的方法
WO2023035003A1 (en) 2021-09-03 2023-03-09 Elegen Corp. Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources
CA3236657A1 (en) 2021-11-10 2023-05-19 Daniel MCHALE Novel terminal deoxynucleotidyl transferase (tdt) variant and uses thereof
WO2023083999A2 (en) 2021-11-10 2023-05-19 Dna Script Novel terminal deoxynucleotidyl transferase (tdt) variants
WO2023170266A1 (en) 2022-03-11 2023-09-14 Dna Script Automation station for enzymatic polynucleotide synthesis
WO2023170286A2 (en) 2022-03-11 2023-09-14 Dna Script Alignment post and secure mechanism for enzymatic polynucleotide synthesis
WO2023170259A1 (en) 2022-03-11 2023-09-14 Dna Script Modular accessory rack
WO2023170258A1 (en) 2022-03-11 2023-09-14 Dna Script Apparatus for enzymatic synthesis of a plurality of polynucleotides comprising a condensation trap
EP4311832A1 (de) * 2022-07-25 2024-01-31 Universität Zürich Photocaged orexin-b
WO2024083883A1 (en) 2022-10-19 2024-04-25 Dna Script Methods and products for removal of uracil containing polynucleotides

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1472937A (en) * 1974-08-12 1977-05-11 Biosedra Lab Benzyl alcohol derivatives
JPS5964635A (ja) * 1982-10-06 1984-04-12 Fujikura Kasei Kk 吸水性樹脂の製造方法
US4593029A (en) * 1984-02-15 1986-06-03 Syntex (U.S.A.) Inc. Novel ω-(N-imidazolyl)alkyl ethers of 1,2,3,5-tetrahydroimidazo[2,1-b]quinazolin-2-ones
DE3565986D1 (en) * 1984-05-02 1988-12-08 Brendan James Hamill An apparatus for the chemical synthesis of oligonucleotides
US4670434A (en) * 1985-11-14 1987-06-02 Syntex (U.S.A.) Inc. (2-oxo-3-methylene-1,2,3,5-tetrahydroimidazo[2,1-b]quinazolinyl)oxyalkylamides useful as cyclic AMP phosphodiesterase inhibitors
AU3869289A (en) * 1988-07-14 1990-02-05 Baylor College Of Medicine Solid phase assembly and reconstruction of biopolymers
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5424186A (en) * 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
AU663300B2 (en) * 1990-12-06 1995-10-05 Affymetrix, Inc. Very large scale immobilized polymer synthesis
DE69230587T2 (de) * 1991-11-15 2000-08-03 Ici Plc Polymerisierbare Zusammensetzung
US5610274A (en) * 1991-11-20 1997-03-11 Cpg, Inc. Production and use of magnetic porous inorganic materials
US5288514A (en) * 1992-09-14 1994-02-22 The Regents Of The University Of California Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
US5482719A (en) * 1992-10-30 1996-01-09 Guillet; James E. Drug delivery systems
GB9315847D0 (en) * 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5549974A (en) * 1994-06-23 1996-08-27 Affymax Technologies Nv Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof
US5679773A (en) * 1995-01-17 1997-10-21 Affymax Technologies N.V Reagants and methods for immobilized polymer synthesis and display

Also Published As

Publication number Publication date
AU689924B2 (en) 1998-04-09
AU2948395A (en) 1996-01-19
US5739386A (en) 1998-04-14
JPH10507160A (ja) 1998-07-14
DE69531545T2 (de) 2004-06-24
US5917016A (en) 1999-06-29
EP0776330A2 (de) 1997-06-04
EP0776330A4 (de) 1999-07-28
CA2193228A1 (en) 1996-01-04
EP0776330B1 (de) 2003-08-20
WO1996000378A1 (en) 1996-01-04

Similar Documents

Publication Publication Date Title
DE69531545D1 (de) Photolabile verbindungen und verfahren für deren verwendung
DE69627831D1 (de) Benzopyran-enthaltende verbindungen und verfahren zu deren verwendung
DE59603890D1 (de) Bürste und verfahren zu deren herstellung
DE69532895D1 (de) Biomaterial und verfahren zu dessen herstellung
DE69504892D1 (de) Mischelement und Verfahren zu dessen Herstellung
DE69511714D1 (de) Fahrzeugfenster und verfahren zu dessen herstellung
DE69705538D1 (de) Abrasive gegenstände und verfahren zu deren herstellung
DE69720523D1 (de) Polyolefin-polyethylenoxid-mischungen und verfahren zu deren herstellung
DE69527801D1 (de) Ultrahochfeste stähle und verfahren zu deren herstellung
DE69523596T2 (de) Verbesserte verbundwerkstoffe und verfahren zu deren herstellung
DE59502879D1 (de) Ausweiskarte und verfahren zu deren herstellung
DE69509814T2 (de) Anzüge und Verfahren zu deren Herstellung
DE69520286T2 (de) Deckschicht für absorbierende artikel und verfahren zu deren herstellung
DE69631354D1 (de) Kreuzströmwabenkörper und Verfahren zu dessen Herstellung
DE69524225D1 (de) Erinnerungsvorrichtung und verfahren zu deren betrieb
DE69525781D1 (de) Brezel und Verfahren zu deren Herstellung
DE69502301T2 (de) Ladesystem und Verfahren zu dessen Herstellung
DE69608359D1 (de) Hexaazaisowurtzitan-derivate und verfahren zu deren herstellung
DE69506678D1 (de) Lüftungsvorrichtung für Schuhe und Verfahren zu deren Herstellung
DE69716562T2 (de) Bürste und Verfahren zu deren Herstellung
DE69422709T2 (de) Längliche zinkoxydpartikel und verfahren zu deren herstellung
DE59502654D1 (de) Mikroelektronisches bauelement und verfahren zu dessen herstellung
DE69620593T2 (de) Hexacyclische camptothecinanaloga und verfahren zu deren herstellung
DE69531127D1 (de) Halbleitervorrichtung und Verfahren zu deren Herstellung
DE69615197D1 (de) Befestigungsvorrichtung und verfahren zu deren herstellung

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee