DE69517105D1 - In vitro transposition von artifiziellen transposons mit stumpfen enden - Google Patents

In vitro transposition von artifiziellen transposons mit stumpfen enden

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Publication number
DE69517105D1
DE69517105D1 DE69517105T DE69517105T DE69517105D1 DE 69517105 D1 DE69517105 D1 DE 69517105D1 DE 69517105 T DE69517105 T DE 69517105T DE 69517105 T DE69517105 T DE 69517105T DE 69517105 D1 DE69517105 D1 DE 69517105D1
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DE
Germany
Prior art keywords
vitro transposition
blunted ends
artificial transposons
transposons
artificial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69517105T
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English (en)
Other versions
DE69517105T2 (de
Inventor
Scott E Devine
Jef D Boeke
Lelita T Braiterman
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Johns Hopkins University
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Johns Hopkins University
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Publication of DE69517105D1 publication Critical patent/DE69517105D1/de
Publication of DE69517105T2 publication Critical patent/DE69517105T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
DE69517105T 1994-03-02 1995-03-02 In vitro transposition von artifiziellen transposons mit stumpfen enden Expired - Fee Related DE69517105T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/204,675 US5677170A (en) 1994-03-02 1994-03-02 In vitro transposition of artificial transposons
PCT/US1995/002520 WO1995023875A1 (en) 1994-03-02 1995-03-02 In vitro transposition of artificial transposons

Publications (2)

Publication Number Publication Date
DE69517105D1 true DE69517105D1 (de) 2000-06-29
DE69517105T2 DE69517105T2 (de) 2000-11-16

Family

ID=22758948

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69517105T Expired - Fee Related DE69517105T2 (de) 1994-03-02 1995-03-02 In vitro transposition von artifiziellen transposons mit stumpfen enden

Country Status (7)

Country Link
US (4) US5677170A (de)
EP (1) EP0698124B1 (de)
JP (1) JP3832851B2 (de)
AU (1) AU682588B2 (de)
CA (1) CA2161767A1 (de)
DE (1) DE69517105T2 (de)
WO (1) WO1995023875A1 (de)

Families Citing this family (170)

* Cited by examiner, † Cited by third party
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DE69517105T2 (de) 2000-11-16
US5728551A (en) 1998-03-17
EP0698124B1 (de) 2000-05-24
US5968785A (en) 1999-10-19
AU682588B2 (en) 1997-10-09
EP0698124A1 (de) 1996-02-28
JPH08510388A (ja) 1996-11-05
CA2161767A1 (en) 1995-09-08
US5843772A (en) 1998-12-01
AU1973995A (en) 1995-09-18

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