DE69511422T2 - Durchflussvorrichtung und verfahren zur elektroporation - Google Patents

Durchflussvorrichtung und verfahren zur elektroporation

Info

Publication number
DE69511422T2
DE69511422T2 DE69511422T DE69511422T DE69511422T2 DE 69511422 T2 DE69511422 T2 DE 69511422T2 DE 69511422 T DE69511422 T DE 69511422T DE 69511422 T DE69511422 T DE 69511422T DE 69511422 T2 DE69511422 T2 DE 69511422T2
Authority
DE
Germany
Prior art keywords
flow
blood
patient
cells
chamber
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69511422T
Other languages
English (en)
Other versions
DE69511422D1 (de
Inventor
Gunter Hofmann
Henry Kent
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetronics Inc
Original Assignee
Genetronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetronics Inc filed Critical Genetronics Inc
Application granted granted Critical
Publication of DE69511422D1 publication Critical patent/DE69511422D1/de
Publication of DE69511422T2 publication Critical patent/DE69511422T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/18—Erythrocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
DE69511422T 1994-10-12 1995-10-12 Durchflussvorrichtung und verfahren zur elektroporation Expired - Fee Related DE69511422T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/320,966 US5545130A (en) 1992-04-08 1994-10-12 Flow through electroporation method
PCT/US1995/014622 WO1996012006A2 (en) 1994-10-12 1995-10-12 Flow through electroporation apparatus and method

Publications (2)

Publication Number Publication Date
DE69511422D1 DE69511422D1 (de) 1999-09-16
DE69511422T2 true DE69511422T2 (de) 2000-02-03

Family

ID=23248604

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69511422T Expired - Fee Related DE69511422T2 (de) 1994-10-12 1995-10-12 Durchflussvorrichtung und verfahren zur elektroporation

Country Status (8)

Country Link
US (2) US5545130A (de)
EP (1) EP0785987B1 (de)
JP (1) JP3740664B2 (de)
AT (1) ATE183231T1 (de)
AU (1) AU691705B2 (de)
CA (1) CA2196610C (de)
DE (1) DE69511422T2 (de)
WO (1) WO1996012006A2 (de)

Families Citing this family (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545130A (en) * 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US6773669B1 (en) 1995-03-10 2004-08-10 Maxcyte, Inc. Flow electroporation chamber and method
US6103084A (en) * 1995-06-06 2000-08-15 Eppendorf Netheler-Hinz Gmbh Apparatus for electroporation
US6041252A (en) * 1995-06-07 2000-03-21 Ichor Medical Systems Inc. Drug delivery system and method
US5879317A (en) * 1996-04-30 1999-03-09 Medtronic, Inc. Electrostatic blood defoamer for heart-lung machines
US5869326A (en) * 1996-09-09 1999-02-09 Genetronics, Inc. Electroporation employing user-configured pulsing scheme
US6090617A (en) * 1996-12-05 2000-07-18 Entremed, Inc. Flow electroporation chamber with electrodes having a crystalline metal nitride coating
US5903211A (en) * 1997-02-07 1999-05-11 Althin Medical, Inc. Medical treatment device with a user interface adapted for home or limited care environments
US5873849A (en) * 1997-04-24 1999-02-23 Ichor Medical Systems, Inc. Electrodes and electrode arrays for generating electroporation inducing electrical fields
US6055453A (en) * 1997-08-01 2000-04-25 Genetronics, Inc. Apparatus for addressing needle array electrodes for electroporation therapy
EP1054694A2 (de) 1998-02-13 2000-11-29 Selective Genetics, Inc. Gleichzeitige strömungsdurchmischung zur herstellung von zusammensetzungen, die gentherapievektoren enthalten
HUP0101828A3 (en) 1998-05-04 2005-08-29 Fraunhofer Ges Forschung Electrical integrated nucleic acid isolation, purification and detection
US6027488A (en) 1998-06-03 2000-02-22 Genetronics, Inc. Flow-through electroporation system for ex vivo gene therapy
US20040229363A1 (en) * 1998-06-24 2004-11-18 Ed Nolan High efficiency transfection based on low electric field strength, long pulse length
US6150148A (en) * 1998-10-21 2000-11-21 Genetronics, Inc. Electroporation apparatus for control of temperature during the process
AU3804100A (en) 1999-02-15 2000-09-04 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Method and sample support system for separating and processing substances in situ
US6326177B1 (en) 1999-08-04 2001-12-04 Eastern Virginia Medical School Of The Medical College Of Hampton Roads Method and apparatus for intracellular electro-manipulation
US6586257B1 (en) 1999-10-12 2003-07-01 Vertex Pharmaceuticals Incorporated Multiwell scanner and scanning method
US6448089B1 (en) 1999-10-12 2002-09-10 Aurora Biosciences Corporation Multiwell scanner and scanning method
US6814933B2 (en) * 2000-09-19 2004-11-09 Aurora Biosciences Corporation Multiwell scanner and scanning method
CA2395492A1 (en) * 1999-12-22 2001-07-05 Diana Wang Method and apparatus for targeting localised electroporation
AU2001241958A1 (en) * 2000-03-03 2001-09-17 Valentis, Inc. Improved poloxamer and poloxamine compositions for nucleic acid delivery
AU6475901A (en) 2000-05-22 2001-12-03 Merck And Company Inc System and method for assessing the performance of a pharmaceutical agent delivery system
US7615356B2 (en) * 2000-07-10 2009-11-10 Vertex Pharmaceuticals (San Diego) Llc Ion channel assay methods
US6686193B2 (en) * 2000-07-10 2004-02-03 Vertex Pharmaceuticals, Inc. High throughput method and system for screening candidate compounds for activity against target ion channels
US20040009940A1 (en) * 2000-10-20 2004-01-15 Coleman Michael E. Gene delivery formulations and methods for treatment of ischemic conditions
US7029916B2 (en) * 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
US20040204669A1 (en) * 2001-07-05 2004-10-14 Hofmann Gunter A. Apparatus for electroporation mediated delivery for drugs and genes
WO2003018751A2 (en) 2001-08-22 2003-03-06 Maxcyte, Inc. Apparatus and method for electroporation of biological samples
WO2003026599A1 (en) * 2001-09-26 2003-04-03 The Procter & Gamble Company Personal cleansing compositions comprising silicone resin-containing adhesives
AU2002348417B9 (en) 2001-10-26 2010-02-04 Baylor College Of Medicine A composition and method to alter lean body mass and bone properties in a subject
AU2002360540A1 (en) * 2001-12-04 2003-06-17 University Of Southern California Method for intracellular modifications within living cells using pulsed electric fields
DE10208188B4 (de) * 2002-02-20 2006-05-24 Amaxa Gmbh Behälter mit zumindest einer Elektrode
CA2477872A1 (en) 2002-03-07 2003-09-18 Merck & Co., Inc. Clinical syringe with electrical stimulation aspects
US7245963B2 (en) * 2002-03-07 2007-07-17 Advisys, Inc. Electrode assembly for constant-current electroporation and use
US8209006B2 (en) * 2002-03-07 2012-06-26 Vgx Pharmaceuticals, Inc. Constant current electroporation device and methods of use
KR20050013531A (ko) * 2002-03-15 2005-02-04 디파트먼트 오브 베테랑스 어페어스 오피스 오브 더 제네럴 카운셀 (024) 세포의 아시알로결정자 및 당접합체를 이용하여 세포를조직 및 기관으로 표적화하는 방법 및 이를 위한 조성물
US20050233993A1 (en) * 2002-06-05 2005-10-20 Kadonaga James T Methods for promoting homologous recombination
US20040115784A1 (en) * 2002-09-30 2004-06-17 Maxcyte, Inc. Apparatus and method for streaming electroporation
JP2006508663A (ja) * 2002-12-03 2006-03-16 ジェネトロニクス，インコーポレイティド 大規模エレクトロポレーションプレート、システム及びその使用方法
US6969604B1 (en) 2003-06-20 2005-11-29 Yakovenko Sergey A Electroporation chamber
WO2005016964A2 (en) 2003-08-04 2005-02-24 Advisys, Inc. Canine specific growth hormone releasing hormone
US7521224B2 (en) * 2003-09-30 2009-04-21 The United States Of America As Represented By The Secretary Of The Navy Microelectronic cell electroporation array
WO2005065722A2 (en) 2003-12-31 2005-07-21 Advisys, Inc. Reducing arthritis and lameness in subjects by growth hormone releasing hormone (ghrh) supplementation
US7517863B2 (en) 2004-01-20 2009-04-14 Vgx Pharmaceuticals, Inc. Enhanced secretion/retention of growth hormone releasing hormone (GHRH) from muscle cells by species-specific signal peptide
US20050282283A1 (en) * 2004-04-19 2005-12-22 Laura Vozza-Brown Electroporation apparatus and methods
JP4937904B2 (ja) * 2004-05-12 2012-05-23 マックスサイト インコーポレーティッド 制御されたフローエレクトロポレーションチャンバーに関連した方法および装置
US20060165668A1 (en) * 2004-12-10 2006-07-27 Liu Linda N Genetically modified tumor cells as cancer vaccines
DE102005029414B4 (de) * 2005-06-24 2009-09-03 Forschungszentrum Karlsruhe Gmbh Verfahren zur Bestimmung des durch Elektroporation bewirkenden Aufschlussgrades biologischer Zellen
JP5826450B2 (ja) * 2005-07-22 2015-12-02 ザ ファウンドリー， エルエルシー 治療薬の送達のためのシステムおよび方法
US8058253B2 (en) 2006-07-06 2011-11-15 Vgx Pharmaceuticals, Inc. Growth hormone releasing hormone treatment to decrease cholesterol levels
DK2066399T3 (en) * 2006-10-17 2019-01-21 Inovio Pharmaceuticals Inc Electroporation devices and methods for using them for the electroporation of cells in mammals
US20120078163A1 (en) 2009-05-27 2012-03-29 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Method of generating connective tissue
EP2603517B1 (de) * 2010-05-12 2023-07-19 Cellectis Dynamische misch- und elektroporationskammer und system dafür
US20120076808A1 (en) 2010-09-27 2012-03-29 China Agricultural University Combined antigen and dna vaccine for preventing and treating autoimmune diseases
CN103239734B (zh) 2012-02-10 2016-02-24 北京艾棣维欣生物技术有限公司 用于预防和/或治疗呼吸道合胞病毒感染的疫苗
WO2014093897A1 (en) 2012-12-13 2014-06-19 The Trustees Of The University Of Pennsylvania Wt1 vaccine
US10364451B2 (en) 2013-05-30 2019-07-30 Duke University Polymer conjugates having reduced antigenicity and methods of using the same
EP3003342B1 (de) 2013-05-30 2022-01-26 Duke University Enzymkatalysierte synthese von ortsspezifischen und stöchiometrischen biomolekül-polymer-konjugaten
US10392611B2 (en) 2013-05-30 2019-08-27 Duke University Polymer conjugates having reduced antigenicity and methods of using the same
WO2015054012A1 (en) 2013-10-07 2015-04-16 The Trustees Of The University Of Pennsylvania Vaccines with interleukin-33 as an adjuvant
US10370664B2 (en) 2013-11-07 2019-08-06 University Of Southern California Use of IKK epsilon inhibitors to activate NFAT and T cell response
EP3068427A1 (de) 2013-11-14 2016-09-21 The Trustees Of The University Of Pennsylvania Hiv-1-env-dna-impfstoff plus proteinboost
KR20160091350A (ko) 2013-11-29 2016-08-02 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 중동 호흡기 증후군 코로나바이러스(MERS-CoV) 백신
KR102395498B1 (ko) 2014-01-06 2022-05-09 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 Pd1 및 pdl1 항체 및 백신 조합 및 면역요법을 위한 이들의 사용
KR20230145241A (ko) 2014-10-01 2023-10-17 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 항원 및 어쥬번트로서 인터류킨-21을 갖는 백신
JP6734283B2 (ja) 2015-01-21 2020-08-05 フレッド ハッチンソン キャンサー リサーチ センター 遺伝子治療用ポイントオブケア及び／又はポータブルプラットフォーム
CN107427567A (zh) 2015-01-29 2017-12-01 宾夕法尼亚大学理事会 检查点抑制剂和疫苗组合物及其用于免疫疗法的用途
US10385115B2 (en) 2015-03-26 2019-08-20 Duke University Fibronectin type III domain-based fusion proteins
CA2994279A1 (en) 2015-08-04 2017-02-09 Duke University Genetically encoded intrinsically disordered stealth polymers for delivery and methods of using same
US11752213B2 (en) 2015-12-21 2023-09-12 Duke University Surfaces having reduced non-specific binding and antigenicity
WO2017112825A2 (en) 2015-12-21 2017-06-29 Duke University Polymer conjugates having reduced antigenicity and methods of using the same
US11246924B2 (en) 2016-04-01 2022-02-15 Duke University Alpha-helical peptide nanofibers as a self-adjuvanting vaccine platform
KR102443064B1 (ko) 2016-04-29 2022-09-14 이노비오 파마수티컬즈, 인크. 제제의 전달을 향상시키기 위한 콘드로이티나제 및/또는 히알루로니다제의 생체내 용도
WO2017210476A1 (en) 2016-06-01 2017-12-07 Duke University Nonfouling biosensors
US10233419B2 (en) 2016-06-30 2019-03-19 Zymergen Inc. Apparatuses and methods for electroporation
EP3512868A4 (de) 2016-09-14 2020-04-15 Duke University Triblock-polypeptid-basierte nanopartikel zur abgabe von hydrophilen arzneimitteln
CN110023326A (zh) 2016-09-23 2019-07-16 杜克大学 具有lcst行为的非结构化无重复多肽
US10376495B2 (en) 2016-11-23 2019-08-13 University Of South Florida Small molecules that mimic or antagonize actions of granulocyte colony-stimulating-factor (G-CSF)
WO2018132732A1 (en) 2017-01-12 2018-07-19 Duke University Genetically encoded lipid-polypeptide hybrid biomaterials that exhibit temperature triggered hierarchical self-assembly
US10813935B2 (en) 2017-02-23 2020-10-27 Transgenex Nanobiotech, Inc. Methods and compositions for treating drug resistance in cancer
US10272052B2 (en) 2017-02-24 2019-04-30 University Of South Florida Compositions and methods for the treatment of tauopathies
WO2018156989A1 (en) 2017-02-24 2018-08-30 University Of South Florida The hsp90 activator aha1 drives production of pathological tau aggregates
WO2018213320A1 (en) 2017-05-15 2018-11-22 Duke University Recombinant production of hybrid lipid-biopolymer materials that self-assemble and encapsulate agents
WO2019006374A1 (en) 2017-06-30 2019-01-03 Duke University ORDER AND DISORDER AS A DESIGN PRINCIPLE FOR STIMULI-SENSITIVE BIOPOLYMER NETWORKS
GB201710973D0 (en) 2017-07-07 2017-08-23 Avacta Life Sciences Ltd Scaffold proteins
US11649275B2 (en) 2018-08-02 2023-05-16 Duke University Dual agonist fusion proteins
KR102232757B1 (ko) * 2018-11-22 2021-03-26 (주)엑솔런스바이오테크놀로지 체외충격파를 이용한 표적물질 전달 장치
EP3993900A4 (de) * 2019-07-02 2023-10-18 Kytopen Corporation Vorrichtungen, systeme und kits zur elektromechanischen abgabe und verwendungsverfahren dafür
US11512314B2 (en) 2019-07-12 2022-11-29 Duke University Amphiphilic polynucleotides
TW202128775A (zh) 2019-10-16 2021-08-01 英商阿法克塔生命科學有限公司 PD-L1抑制劑-TGFβ抑制劑雙特異性藥物部分
AU2020370481A1 (en) * 2019-10-25 2022-05-26 Life Technologies Corporation Systems and devices for electroporation of cell-containing fluid
EP4110468A1 (de) 2020-02-25 2023-01-04 Inovio Pharmaceuticals, Inc. Impfstoffe gegen coronavirus und verfahren zur verwendung
CA3177949A1 (en) 2020-05-14 2021-11-18 Stephanie RAMOS Vaccines for recurrent respiratory papillomatosis and methods of using the same
GB202101299D0 (en) 2020-06-09 2021-03-17 Avacta Life Sciences Ltd Diagnostic polypetides and methods
EP4165183A1 (de) 2020-06-12 2023-04-19 University of Rochester Codierung und expression von ace-trnas
USD965170S1 (en) 2020-10-23 2022-09-27 Life Technologies Corporation Electroporation device
WO2022234003A1 (en) 2021-05-07 2022-11-10 Avacta Life Sciences Limited Cd33 binding polypeptides with stefin a protein
TW202332694A (zh) 2021-10-07 2023-08-16 英商阿凡克塔生命科學公司 血清半衰期延長之ｐｄ－ｌ１結合多肽
WO2023057567A1 (en) 2021-10-07 2023-04-13 Avacta Life Sciences Limited Pd-l1 binding affimers
WO2023150753A1 (en) 2022-02-07 2023-08-10 University Of Rochester Optimized sequences for enhanced trna expression or/and nonsense mutation suppression

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4464166A (en) * 1981-06-12 1984-08-07 Frederic A. Bourke, Jr. Method for externally treating the blood
US4612007A (en) * 1981-06-16 1986-09-16 Edelson Richard Leslie Method and system for externally treating the blood
US4496443A (en) * 1982-06-08 1985-01-29 Mack Michael H Method for electrically introducing substances into liquid solution
DE3317415A1 (de) * 1983-05-13 1984-11-15 Kernforschungsanlage Jülich GmbH, 5170 Jülich Kammer zur behandlung von zellen im elektrischen feld
US4561961A (en) * 1984-07-27 1985-12-31 Biotronics Cooled microscope slide and electrode apparatus for use in live cell fusion system
US4849089A (en) * 1986-05-09 1989-07-18 Electropore, Inc. Disposable electromanipulation chamber
US5098843A (en) * 1987-06-04 1992-03-24 Calvin Noel M Apparatus for the high efficiency transformation of living cells
US5128257A (en) * 1987-08-31 1992-07-07 Baer Bradford W Electroporation apparatus and process
US4970154A (en) * 1987-10-09 1990-11-13 Baylor College Of Medicine Method for inserting foreign genes into cells using pulsed radiofrequency
US4832814A (en) * 1987-12-28 1989-05-23 E. I. Du Pont De Nemours And Company Electrofusion cell and method of making the same
US5545130A (en) * 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US5501662A (en) * 1992-05-22 1996-03-26 Genetronics, Inc. Implantable electroporation method and apparatus for drug and gene delivery
US5464386A (en) * 1992-08-17 1995-11-07 Genetronics, Inc. Transdermal drug delivery by electroincorporation of vesicles
DE69433933T2 (de) * 1993-03-23 2005-07-21 CBR Laboratories, Inc., Boston Verfahren und vorrichtung zur einkapselung biologisch aktiver substanzen in zellen

Also Published As

Publication number Publication date
WO1996012006A3 (en) 1996-08-01
US5545130A (en) 1996-08-13
EP0785987A2 (de) 1997-07-30
JP3740664B2 (ja) 2006-02-01
ATE183231T1 (de) 1999-08-15
EP0785987B1 (de) 1999-08-11
AU691705B2 (en) 1998-05-21
US5676646A (en) 1997-10-14
AU4153696A (en) 1996-05-06
WO1996012006A2 (en) 1996-04-25
CA2196610C (en) 2004-07-20
CA2196610A1 (en) 1996-04-25
DE69511422D1 (de) 1999-09-16
JPH10507364A (ja) 1998-07-21

Similar Documents

Publication Publication Date Title
DE69511422T2 (de) Durchflussvorrichtung und verfahren zur elektroporation
ES2117871T3 (es) Terapia electroporetica mediante genes y medicamentos, por campos electricos inducidos.
US6027488A (en) Flow-through electroporation system for ex vivo gene therapy
DE69530283T2 (de) Elektroporation und iontophorese vorrichtung für die abgabe von medikamenten und genen in zellen
US5135478A (en) Multi-signal electrical transdermal drug applicator
US20020147424A1 (en) Transdermal magnetic drug delivery system and method
JPS5573271A (en) Nonnpermeation type chemical sample injection method and its device
US6361665B1 (en) Device for electroactivating fluids and preparations consisting of electroactivated fluids
DE69929303D1 (de) Wegwerfset zum umlaufwaschen von blutzellen
CA2266347A1 (en) Electroporation and iontophoresis catheter with porous balloon
DE59914211D1 (de) Vorrichtung zur bestimmung des verteilungsvolumens eines blutinhaltsstoffes während einer blutbehandlung
WO2004076857A3 (en) Method and apparatus for low-cost electrokinetic pump manufacturing
ATE216155T1 (de) Elektroporationsgerät mit benutzerkonfigurierten impulsen
ATE176601T1 (de) Vorrichtung zur trennung einer mischung
ATE129570T1 (de) Vorrichtung zum feststellen der viskositätsänderung eines flüssigen elektrolyts mittels depolarisierungswirkung.
DE69018966D1 (de) Verfahren zum kontinuierlichen überwachen der arbeitsweise eines ausgabesystems und vorrichtung zur durchführung des verfahrens.
ATE38721T1 (de) Verfahren zum ueberwachen einer gaskonzentration in einer fluessigkeit.
DE60034757D1 (de) Verbesserte Vorrichtung zur ohmischen Heizung eines flüssigen Körpers, Behandlungsanlage die selbige benutzt, und Behandlungsverfahren eines flüssigen Körpers mittels ohmischer Heizung
DE69617908T2 (de) Methode und vorrichtung zur zuführung von elektromagnetischer energie in einer lösung
EP0569634A1 (de) Elektrisches System mit mehreren Signalen zur transdermalen Medikamentenverabreichung
DE60128144D1 (de) Vorrichtung und verfahren zur trennung von molekülen und bewegung von fluiden mittels elektrophorese
RU2008119500A (ru) Устройство мониторинга образования тромба и способ мониторинга образования тромба
DE60220289D1 (de) Verfahren und vorrichtung zur behandlung von flüssigkeiten
JPS5451980A (en) Device of regulating quantity of ultrafiltration
RU94043399A (ru) Способ электро-физико-химической обработки жидкости и/или жидкостно-газовой среды

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee