DE69432787D1 - Klonierung von Enterokinase und Verfahren zu deren Verwendung - Google Patents

Klonierung von Enterokinase und Verfahren zu deren Verwendung

Info

Publication number
DE69432787D1
DE69432787D1 DE69432787T DE69432787T DE69432787D1 DE 69432787 D1 DE69432787 D1 DE 69432787D1 DE 69432787 T DE69432787 T DE 69432787T DE 69432787 T DE69432787 T DE 69432787T DE 69432787 D1 DE69432787 D1 DE 69432787D1
Authority
DE
Germany
Prior art keywords
enterokinase
cloning
methods
nucleic acid
acid sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69432787T
Other languages
English (en)
Inventor
Edward R Lavallie
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetics Institute LLC
Original Assignee
Genetics Institute LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Institute LLC filed Critical Genetics Institute LLC
Application granted granted Critical
Publication of DE69432787D1 publication Critical patent/DE69432787D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Nutrition Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69432787T 1993-01-15 1994-01-13 Klonierung von Enterokinase und Verfahren zu deren Verwendung Expired - Lifetime DE69432787D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US594493A 1993-01-15 1993-01-15
PCT/US1994/000616 WO1994016083A1 (en) 1993-01-15 1994-01-13 Cloning of enterokinase and method of use

Publications (1)

Publication Number Publication Date
DE69432787D1 true DE69432787D1 (de) 2003-07-10

Family

ID=21718492

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69432787T Expired - Lifetime DE69432787D1 (de) 1993-01-15 1994-01-13 Klonierung von Enterokinase und Verfahren zu deren Verwendung

Country Status (8)

Country Link
US (1) US5665566A (de)
EP (1) EP0679189B1 (de)
JP (1) JP3149185B2 (de)
AT (1) ATE242330T1 (de)
AU (1) AU675506B2 (de)
CA (1) CA2153254C (de)
DE (1) DE69432787D1 (de)
WO (1) WO1994016083A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5968782A (en) * 1996-12-04 1999-10-19 Brigham And Womens's Hospital, Inc. Mast cell protease that cleaves fibrinogen
US5955431A (en) * 1997-02-05 1999-09-21 Brigham And Women's Hospital, Inc. Mast cell protease peptide inhibitors
WO1998049326A1 (en) * 1997-04-25 1998-11-05 Sembiosys Genetics Inc. Method for cleavage of fusion proteins
US7037667B1 (en) 1998-06-01 2006-05-02 Agensys, Inc. Tumor antigen useful in diagnosis and therapy of prostate and colon cancer
DE69943020D1 (de) * 1998-06-01 2011-01-20 Agensys Inc Tumorantigen verwendbar in der diagnose und therapie von prostata und dickdarm-krebs
US6461812B2 (en) 1998-09-09 2002-10-08 Agilent Technologies, Inc. Method and multiple reservoir apparatus for fabrication of biomolecular arrays
JP2003531567A (ja) * 1999-04-30 2003-10-28 オーソ−マクニール・フアーマシユーチカル・インコーポレーテツド チモーゲン活性化系
US6420157B1 (en) 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
US6485957B1 (en) 1999-04-30 2002-11-26 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease EOS
US6426199B1 (en) 1999-08-31 2002-07-30 Ortho-Mcneil Pharmaceutical, Inc. Dna
US6458564B1 (en) 1999-08-31 2002-10-01 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease T
US20050158838A1 (en) * 2000-06-19 2005-07-21 Dyax Corp., A Delaware Corporation Novel enterokinase cleavage sequences
CA2408630A1 (en) 2000-06-19 2001-12-27 Dyax Corp. Novel enterokinase cleavage sequences
US6750034B1 (en) 2000-06-30 2004-06-15 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding human serine protease D-G
US20050106700A1 (en) * 2001-10-11 2005-05-19 Tsuyoshi Nomura Method of purifying recombinant fused protein and method of producing protein using the same
KR100507980B1 (ko) * 2002-06-18 2005-08-17 프로테온 주식회사 엔테로키나제 경사슬의 아미노말단 또는 카르복실말단이 변형된 재조합 엔테로키나제
PL213309B1 (pl) 2006-02-09 2013-02-28 Polska Akademia Nauk Inst Biochemii I Biofizyki Sposób hydrolizy wiazania peptydowego
WO2008136014A1 (en) * 2007-05-07 2008-11-13 Usv Limited Cloning and expression of enterokinase and its process for production
EP2376619A4 (de) 2008-12-15 2012-07-04 Greenlight Biosciences Inc Verfahren zur flusssteuerung bei stoffwechselwegen
CN102822196B (zh) 2010-01-25 2014-12-17 阿勒根公司 将单链蛋白质细胞内转化成它们的双链形式的方法
JP2013526277A (ja) 2010-05-07 2013-06-24 グリーンライト バイオサイエンシーズ インコーポレーテッド 酵素リロケーションにより代謝経路のフラックスを制御する方法
EP3219796B1 (de) * 2010-08-31 2020-10-07 GreenLight Biosciences, Inc. Verfahren zur flusssteuerung in stoffwechselwegen durch proteasemanipulation
RU2495934C2 (ru) * 2011-05-30 2013-10-20 Общество с ограниченной ответственностью "Лаборатория медицинской биотехнологии" (ООО "ЛМБТ") ПЛАЗМИДА ДЛЯ ЭКСПРЕССИИ В КЛЕТКАХ БАКТЕРИИ РОДА Escherichia НЕАКТИВНОГО ПРЕДШЕСТВЕННИКА МУТЕИНА [C112S] ЛЕГКОЙ ЦЕПИ ЭНТЕРОКИНАЗЫ ЧЕЛОВЕКА, БАКТЕРИЯ, ПРИНАДЛЕЖАЩАЯ К РОДУ Escherichia, - ПРОДУЦЕНТ ПРЕДШЕСТВЕННИКА РЕКОМБИНАНТНОГО МУТЕИНА [C112S] ЛЕГКОЙ ЦЕПИ ЭНТЕРОКИНАЗЫ ЧЕЛОВЕКА, ПРЕДШЕСТВЕННИК РЕКОМБИНАНТНОГО МУТЕИНА [C112S] ЛЕГКОЙ ЦЕПИ ЭНТЕРОКИНАЗЫ ЧЕЛОВЕКА, СПОСОБ ПОЛУЧЕНИЯ РЕКОМБИНАНТНОГО МУТЕИНА [C112S] ЛЕГКОЙ ЦЕПИ ЭНТЕРОКИНАЗЫ ЧЕЛОВЕКА, РЕКОМБИНАНТНЫЙ МУТЕИН [C112S] ЛЕГКОЙ ЦЕПИ ЭНТЕРОКИНАЗЫ ЧЕЛОВЕКА
CA2846893A1 (en) 2011-09-09 2013-03-14 Greenlight Biosciences, Inc. Cell-free preparation of carbapenems
WO2013092855A1 (en) 2011-12-23 2013-06-27 Novo Nordisk A/S Modified enterokinase light chain
US9611487B2 (en) 2012-12-21 2017-04-04 Greenlight Biosciences, Inc. Cell-free system for converting methane into fuel and chemical compounds
US9688977B2 (en) 2013-08-05 2017-06-27 Greenlight Biosciences, Inc. Engineered phosphoglucose isomerase proteins with a protease cleavage site
MY193444A (en) 2015-03-30 2022-10-13 Greenlight Biosciences Inc Cell-free production of ribonucleic acid
CR20180525A (es) 2016-04-06 2019-02-14 Greenlight Biosciences Inc Producción de ácido ribonucleico libre de células
KR102571743B1 (ko) 2017-10-11 2023-08-29 그린라이트 바이오사이언시스, 아이엔씨. 뉴클레오시드 트리포스페이트 및 리보핵산 생산을 위한 방법 및 조성물

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA769697A (en) * 1967-10-17 Ercoli Nicholas Anti inflammation therapeutic composition
US4769326A (en) * 1980-02-29 1988-09-06 The Regents Of The University Of California Expression linkers
US4745069A (en) * 1982-05-25 1988-05-17 Eli Lilly And Company Cloning vectors for expression of exogenous protein
GB8312339D0 (en) * 1983-05-05 1983-06-08 Austen B M Peptides
US4870008A (en) * 1983-08-12 1989-09-26 Chiron Corporation Secretory expression in eukaryotes
DK122687A (da) * 1986-03-14 1987-09-15 Takeda Chemical Industries Ltd Polypeptid, dna og anvendelse deraf
US4828988A (en) * 1986-05-15 1989-05-09 Smith Kline - Rit Hybrid polypeptides comprising somatocrinine and alpha1 -antitrypsin, method for their production from bacterial clones and use thereof for the production of somatocrinine
NZ220917A (en) * 1986-07-11 1991-05-28 Merck & Co Inc Human and bovine acidic fibroblast growth factor vectors, hosts and compositions
GR871029B (en) * 1986-07-14 1987-11-02 Genetics Inst Novel osteoinductive factors
US5270181A (en) * 1991-02-06 1993-12-14 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin and thioredoxin-like molecules
US5292646A (en) * 1991-02-06 1994-03-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin and thioredoxin-like molecules

Also Published As

Publication number Publication date
CA2153254A1 (en) 1994-07-21
EP0679189A1 (de) 1995-11-02
CA2153254C (en) 2002-05-21
JP3149185B2 (ja) 2001-03-26
AU675506B2 (en) 1997-02-06
US5665566A (en) 1997-09-09
JPH08502176A (ja) 1996-03-12
ATE242330T1 (de) 2003-06-15
WO1994016083A1 (en) 1994-07-21
AU6089894A (en) 1994-08-15
EP0679189B1 (de) 2003-06-04

Similar Documents

Publication Publication Date Title
ATE242330T1 (de) Klonierung von enterokinase und verfahren zu deren verwendung
DE3381783D1 (de) Menschliches antithrombin iii, dns sequenzen dafuer, expressions- und klonierungsvektoren die solche sequenzen enthalten und damit transformierte zellkulturen, verfahren zur expression von menschlichem antithrombin iii und diese enthaltende pharmazeutische zusammensetzungen.
DE3587597D1 (de) Lymphotoxin, dafuer kodierende nukleinsaeure, die nukleinsaeure enthaltenden vektoren und damit transformierte zellen, verfahren zum erhalten von lymphotoxin und lymphotoxin neutralisierender antikoerper.
DE3582576D1 (de) Dithioketo-pyrrolo-pyrrole, verfahren zu deren herstellung und verwendung.
FI951612A (fi) Menetelmiä synteettisten oligonukleotidien sekvensoimiseksi, jotka sisältävät nukleotidien välisiä ei-fosfodiesterisidoksia
DE3878326D1 (de) Kunstlunge der membranart und verfahren zu ihrer verwendung.
DE69322007T2 (de) Carboxy substituierte phenylgruppe enthaltende carbapeneme; verfahren zu deren herstellung, zwischenprodukte und verwendung als antibiotika
DE3885440D1 (de) Haftgrundmittel für Proteinsubstrate, Verfahren zu deren Herstellung und Verwendung.
DE69232537T2 (de) Rezeptoren der steroid/thyroid superfamilie von rezeptoren
ATE223489T1 (de) Rekombinante hexose oxidase, verfahren zu deren herstellung und verwendung
DE69434614D1 (de) Cdn apoptosis modulierende proteine, sie kodierende dns und verfahren zu ihrer verwendung
DE3682928D1 (de) Verfahren zum polymerisieren von aethylen.
DE69019753T3 (de) Verfahren zur verwendung von gashilfe während des giessens von kunststoffgegenständen.
DE3773318D1 (de) Titanoxid-katalysator, verfahren zu dessen herstellung und dessen verwendung fuer die herstellung von linearen polyaethylenpolyaminen.
DE3878228D1 (de) Verfahren zur reinigung von stickstoff-fluorid.
DE69018625D1 (de) Verfahren zum aufkonzentrieren von chlorgas.
DE3580875D1 (de) Pyrimidin-derivate, verfahren zu deren herstellung und diese enthaltende zusammenstellungen.
DE3778148D1 (de) Zusammensetzungen mit discorhabdin, sowie verfahren zu deren verwendung.
DE3686969D1 (de) Haertbare zusammensetzung, verfahren zu ihrer herstellung und daraus gefertigte gegenstaende.
DE69423574T2 (de) Verfahren zum Lysieren von Mycobakterien
DE3676002D1 (de) Quaternaere oxalkylierte polykondensate, verfahren zu deren herstellung und deren verwendung.
DE3574701D1 (de) Pentaerythritdiphosphite, verfahren zu deren herstellung und deren verwendung als stabilisatoren.
DE3679028D1 (de) Benzylaminderivate, verfahren zu ihrer herstellung und ihre verwendung.
DE69202111D1 (de) Verfahren zum Lysieren von Mykobakterien.
DE3887369D1 (de) Wässriges Überzugsmittel, Verfahren zu dessen Herstellung und dessen Verwendung zum elektrophoretischen Beschichten.

Legal Events

Date Code Title Description
8332 No legal effect for de