DE69421582T2 - Verfahren für nicht-kompetitive Bindungsassays - Google Patents
Verfahren für nicht-kompetitive BindungsassaysInfo
- Publication number
- DE69421582T2 DE69421582T2 DE69421582T DE69421582T DE69421582T2 DE 69421582 T2 DE69421582 T2 DE 69421582T2 DE 69421582 T DE69421582 T DE 69421582T DE 69421582 T DE69421582 T DE 69421582T DE 69421582 T2 DE69421582 T2 DE 69421582T2
- Authority
- DE
- Germany
- Prior art keywords
- analyte
- binder
- bound
- labelled specific
- label
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000012875 competitive assay Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 230000036963 noncompetitive effect Effects 0.000 title 1
- 239000011230 binding agent Substances 0.000 abstract 12
- 239000012491 analyte Substances 0.000 abstract 8
- 239000002198 insoluble material Substances 0.000 abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract 2
- 238000005259 measurement Methods 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 239000000463 material Substances 0.000 abstract 1
- 230000003278 mimic effect Effects 0.000 abstract 1
- 238000000159 protein binding assay Methods 0.000 abstract 1
- 239000012088 reference solution Substances 0.000 abstract 1
- 239000007790 solid phase Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0078—Testing material properties on manufactured objects
- G01N33/008—Sport articles, e.g. balls, skis or rackets
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biophysics (AREA)
- Urology & Nephrology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Steroid Compounds (AREA)
- Drying Of Semiconductors (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Testing Of Optical Devices Or Fibers (AREA)
- Bridges Or Land Bridges (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/121,806 US5445936A (en) | 1993-09-15 | 1993-09-15 | Method for non-competitive binding assays |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69421582D1 DE69421582D1 (de) | 1999-12-16 |
DE69421582T2 true DE69421582T2 (de) | 2000-02-17 |
Family
ID=22398918
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69421582T Expired - Fee Related DE69421582T2 (de) | 1993-09-15 | 1994-09-13 | Verfahren für nicht-kompetitive Bindungsassays |
Country Status (9)
Country | Link |
---|---|
US (2) | US5445936A (de) |
EP (1) | EP0643306B1 (de) |
JP (1) | JP3364537B2 (de) |
KR (1) | KR950009259A (de) |
AT (1) | ATE186599T1 (de) |
AU (1) | AU680270B2 (de) |
CA (1) | CA2117597A1 (de) |
DE (1) | DE69421582T2 (de) |
PL (1) | PL304988A1 (de) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10200374A1 (de) * | 2002-01-08 | 2003-07-24 | Friz Biochem Gmbh | Verfahren zur qualitativ und quantitativ vergleichenden Detektion chemischer Substanzen |
Families Citing this family (38)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5445936A (en) * | 1993-09-15 | 1995-08-29 | Ciba Corning Diagnostics Corp. | Method for non-competitive binding assays |
ES2204960T3 (es) * | 1994-06-24 | 2004-05-01 | Dade Behring Marburg Gmbh | Procedimiento para la estabilizacion de moleculas sensibles a la hidrolisis. |
DE4440487A1 (de) * | 1994-11-12 | 1996-05-15 | Behringwerke Ag | Heterogener Immunoassay mittels präzipitierbarer Festphase |
DE19524414A1 (de) * | 1995-07-05 | 1997-01-09 | Behringwerke Ag | Antigenüberschußfreie nephelometrische und turbidimetrische Proteinbestimmungen |
US5817527A (en) * | 1995-11-06 | 1998-10-06 | Chiron Diagnostics Corporation | Conjugation of ligand to immobilized protein in organic solvent |
KR970025944A (ko) * | 1995-11-24 | 1997-06-24 | 김준웅 | 옥외광고판용 원단 |
KR970025947A (ko) * | 1995-11-30 | 1997-06-24 | 김준웅 | 옥외 광고판용 원단 |
US5856194A (en) | 1996-09-19 | 1999-01-05 | Abbott Laboratories | Method for determination of item of interest in a sample |
US5795784A (en) | 1996-09-19 | 1998-08-18 | Abbott Laboratories | Method of performing a process for determining an item of interest in a sample |
US6165800A (en) * | 1997-05-30 | 2000-12-26 | Bayer Corporation | Chemiluminescent energy transfer conjugates and their uses as labels in binding assays |
US6174667B1 (en) * | 1997-09-23 | 2001-01-16 | Cornell Research Foundation, Inc. | Bovine viral diarrhea virus serum antigen capture |
AU9673198A (en) * | 1997-10-02 | 1999-04-27 | Aclara Biosciences, Inc. | Capillary assays involving separation of free and bound species |
ATE286251T1 (de) | 1999-01-27 | 2005-01-15 | Bayer Ag | Immuntest unter verwendung von partikeln mit kaseinumhüllungen |
US6127140A (en) * | 1999-06-18 | 2000-10-03 | Abbott Laboratories | Assay for quantitative measurement of analytes in biological samples |
AU3986501A (en) * | 2000-02-23 | 2001-09-03 | Zyomyx Inc | Chips having elevated sample surfaces |
ATE335202T1 (de) * | 2000-03-16 | 2006-08-15 | Biacore Ab | Verfahren zur erfassung von analyten, die aus oberflächengebundenen liganden eluiert werden |
US6797481B1 (en) | 2000-10-17 | 2004-09-28 | Dade Behring Marburg Gmbh | Simultaneous screening of multiple analytes |
AU2002259117A1 (en) * | 2001-05-03 | 2002-11-18 | Immunetics, Inc. | Systems and methods for detection of analytes in biological fluids |
US7262019B2 (en) * | 2001-05-03 | 2007-08-28 | Immunetics, Inc. | System and methods for detection of Bacillus anthracis related analytes in biological fluids |
US7449288B2 (en) * | 2001-08-09 | 2008-11-11 | Cornell Research Foundation, Inc. | Detection of bovine viral diarrhea virus in tissue samples |
US7459269B2 (en) * | 2001-08-09 | 2008-12-02 | Cornell Research Foundation, Inc. | Detection of bovine viral diarrhea virus in hair samples |
US6723851B2 (en) * | 2001-10-31 | 2004-04-20 | Quest Diagnostics Investment Incorporated | Chemiluminescent compounds and use thereof |
US20030153011A1 (en) * | 2002-02-08 | 2003-08-14 | Bell Michael L. | Methods and reagents for conducting multiplexed assays of multiple analytes |
US20040053308A1 (en) * | 2002-06-28 | 2004-03-18 | Canon Kabushiki Kaisha | Probe immobilized substrate and method for manufacturing the same, and analytical method |
US7108993B2 (en) * | 2002-07-19 | 2006-09-19 | Bayer Healthcare Llc | Use of dual conjugated labels in the elimination of serum interference in immunochromatographic assays |
US7341837B2 (en) * | 2003-09-02 | 2008-03-11 | Lawton Robert L | Soluble analyte detection and amplification |
US20050112586A1 (en) * | 2003-11-24 | 2005-05-26 | Roland Janzen | Method and composition for stabilizing liquid reagents |
US7329391B2 (en) * | 2003-12-08 | 2008-02-12 | Applera Corporation | Microfluidic device and material manipulating method using same |
KR100639776B1 (ko) * | 2004-01-05 | 2006-10-27 | 바이오메드포토닉스 주식회사 | 측방 유동 정량 검정 방법 및 이를 위한 스트립과 레이저유발 표면형광 검출 장치 및 소형 스캐너 |
KR100572173B1 (ko) * | 2004-11-12 | 2006-04-18 | 한화폴리드리머 주식회사 | 방오성 및 내후성 향상용 천막지의 제조방법 |
US7172906B2 (en) * | 2004-11-16 | 2007-02-06 | Dade Behring Inc. | Reduction of non-specific binding in assays |
EP1849001B1 (de) * | 2005-02-18 | 2016-04-06 | Charm Sciences, Inc. | Seitenstrom-testsatz und verfahren zum nachweis eines analyten |
JP5055289B2 (ja) * | 2005-11-17 | 2012-10-24 | シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレイテッド | アッセイにおける非特異的結合の低減 |
US20090246795A1 (en) * | 2005-12-22 | 2009-10-01 | Rohm Co., Ltd. | Immunoassay device and method |
US20110262989A1 (en) * | 2010-04-21 | 2011-10-27 | Nanomr, Inc. | Isolating a target analyte from a body fluid |
US10465226B2 (en) | 2014-10-16 | 2019-11-05 | The General Hospital Corporation | Devices and methods for target analyte detection in liquid samples |
KR102614839B1 (ko) | 2016-04-15 | 2023-12-15 | 버클리 라잇츠, 인크. | 펜 내 분석들을 위한 방법들, 시스템들 및 키트들 |
EP3721209B1 (de) | 2017-10-15 | 2024-02-07 | Berkeley Lights, Inc. | Verfahren für in-pen-tests |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3654090A (en) * | 1968-09-24 | 1972-04-04 | Organon | Method for the determination of antigens and antibodies |
US4434236A (en) * | 1982-10-20 | 1984-02-28 | E. I. Du Pont De Nemours & Co. | Immunoassay wherein labeled antibody is displaced from immobilized analyte-analogue |
NO843838L (no) * | 1983-09-26 | 1985-03-27 | Ortho Diagnostic Systems Inc | Fremgangsmaate for detektering av nukleinsyre |
US4551426A (en) * | 1983-10-03 | 1985-11-05 | E. I. Du Pont De Nemours And Company | Heterogeneous immunoassay for digoxin using ouabain as a separation means |
DE3433652A1 (de) * | 1984-09-13 | 1986-03-20 | Boehringer Mannheim Gmbh, 6800 Mannheim | Immunchemisches messverfahren fuer haptene und proteine |
US4788136A (en) * | 1987-04-07 | 1988-11-29 | Abbott Laboratories | Diagnostic immunoassay by solid phase separation for digoxin |
US5236849A (en) * | 1987-08-11 | 1993-08-17 | Eiji Ishikawa | Method of high sensitivity immunoassay |
WO1991016116A1 (en) * | 1990-04-23 | 1991-10-31 | Cellpro Incorporated | Immunoselection device and method |
US5451504A (en) * | 1991-07-29 | 1995-09-19 | Serex, Inc. | Method and device for detecting the presence of analyte in a sample |
US5312730A (en) * | 1992-05-27 | 1994-05-17 | Ciba Corning Diagnostics Corp. | Immune complex transfer with lypophilic bridge |
US5445936A (en) * | 1993-09-15 | 1995-08-29 | Ciba Corning Diagnostics Corp. | Method for non-competitive binding assays |
-
1993
- 1993-09-15 US US08/121,806 patent/US5445936A/en not_active Expired - Lifetime
-
1994
- 1994-08-29 CA CA002117597A patent/CA2117597A1/en not_active Abandoned
- 1994-08-31 AU AU71576/94A patent/AU680270B2/en not_active Ceased
- 1994-09-09 PL PL94304988A patent/PL304988A1/xx unknown
- 1994-09-13 DE DE69421582T patent/DE69421582T2/de not_active Expired - Fee Related
- 1994-09-13 AT AT94306702T patent/ATE186599T1/de active
- 1994-09-13 EP EP94306702A patent/EP0643306B1/de not_active Expired - Lifetime
- 1994-09-14 KR KR1019940023263A patent/KR950009259A/ko not_active Application Discontinuation
- 1994-09-14 JP JP22018694A patent/JP3364537B2/ja not_active Expired - Fee Related
-
1995
- 1995-05-04 US US08/434,743 patent/US5705338A/en not_active Expired - Lifetime
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE10200374A1 (de) * | 2002-01-08 | 2003-07-24 | Friz Biochem Gmbh | Verfahren zur qualitativ und quantitativ vergleichenden Detektion chemischer Substanzen |
Also Published As
Publication number | Publication date |
---|---|
EP0643306A3 (de) | 1996-01-24 |
AU7157694A (en) | 1995-03-30 |
US5705338A (en) | 1998-01-06 |
PL304988A1 (en) | 1995-03-20 |
EP0643306B1 (de) | 1999-11-10 |
JP3364537B2 (ja) | 2003-01-08 |
AU680270B2 (en) | 1997-07-24 |
DE69421582D1 (de) | 1999-12-16 |
KR950009259A (ko) | 1995-04-21 |
EP0643306A2 (de) | 1995-03-15 |
US5445936A (en) | 1995-08-29 |
JPH07151757A (ja) | 1995-06-16 |
CA2117597A1 (en) | 1995-03-16 |
ATE186599T1 (de) | 1999-11-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |